RESUMO
HLA-DRB1*07:147Q differs from HLA-DRB1*07:01:01:01 by one nucleotide substitution leading to a premature stop codon in exon 4.
Assuntos
Transplante de Rim , Humanos , Cadeias HLA-DRB1/genética , Alelos , Éxons/genética , Sequenciamento de Nucleotídeos em Larga EscalaRESUMO
HLA-DPB1*1584:01 differs from HLA-DPB1*104:01:01:03 by one nucleotide substitution in exon 2.
Assuntos
Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Sequência de Bases , Alelos , Cadeias beta de HLA-DP/genéticaRESUMO
The novel HLA allele HLA-A*31:215 differs from HLA-A*31:01:02:01 by one nucleotide substitution c.G832A in exon 4.
Assuntos
Sequenciamento de Nucleotídeos em Larga Escala , Nucleotídeos , Humanos , Alelos , Éxons , Antígenos HLA-ARESUMO
The novel HLA-C*04:489 and -C*07:01:115 alleles were detected during routine HLA typing by next-generation sequencing.
Assuntos
Antígenos HLA , Antígenos HLA-C , Humanos , Antígenos HLA-C/genética , Alelos , Genes MHC Classe I , Sequenciamento de Nucleotídeos em Larga EscalaRESUMO
The novel HLA-allele B*14:110, differs from B*14:02:01:01, by one nucleotide substitution, c.T247A in exon 2.
Assuntos
Antígenos HLA-B , Sequenciamento de Nucleotídeos em Larga Escala , Alelos , Antígenos HLA-B/genética , Teste de Histocompatibilidade , Humanos , NucleotídeosRESUMO
Short-term allograft survival has improved among solid organ transplant (SOT) patients. An increasing number of SOT patients are prepared for re-transplantation because of chronic allograft failure. Lack of HLA typing or incomplete HLA typing of previous donors complicates pretransplant risk assessment, as repeated HLA mismatches may be missed. In addition, a complete HLA type of the donor is essential in the diagnosis of antibody-mediated rejection. We aimed to determine donor HLA types from allograft biopsies from kidney, heart and liver grafts. Graft biopsies were obtained from 13 kidney, heart and liver transplanted patients. HLA typing was performed using q-PCR. Alleles of both donor and recipient origin were detected, and donor HLA type was concluded by deducting known HLA types of the recipient. For all 13 patients, we were able to determine mismatched donor HLA alleles from graft material. These results are promising, because they enable better individualized risk assessment.