RESUMO
A 14-year-old girl presented to our hospital with food-dependent exercise-induced anaphylaxis (FDEIA), possibly caused by rice. Despite experiencing four previous episodes of suspected FDEIA, she did not seek medical attention at her own discretion. On the fifth occurrence of symptoms, the general practitioner suspected FDEIA and referred the patient to our hospital. The only common factor in all five episodes was the consumption of rice, leading to the examination of the patient under suspicion of FDEIA caused by rice. Skin prick test results were positive for bran and polished rice, and exercise after consumption of polished rice resulted in anaphylaxis. Therefore, we diagnosed FDEIA caused by polished rice. Immunoblotting confirmed the presence of immunoglobulin E reacting with 14-16kDa rice bran protein in the patient's serum. The immunoblot inhibition test suggested that the rice bran protein to which the patient's serum reacted was also present in polished rice and no wash rice. As the patient may experience FDEIA after ingestion of no wash rice or rice flour, she was advised to eliminate these from her diet, treating them similarly to brown rice or polished rice.
Assuntos
Anafilaxia , Exercício Físico , Hipersensibilidade Alimentar , Oryza , Humanos , Feminino , Oryza/imunologia , Oryza/efeitos adversos , Anafilaxia/etiologia , Anafilaxia/imunologia , Adolescente , Hipersensibilidade Alimentar/imunologia , Hipersensibilidade Alimentar/etiologia , Imunoglobulina E/imunologia , Alergias Induzidas por ExercícioRESUMO
BACKGROUND: The purpose of this study was to compare the antigenicity of Bonlact® i (BL) with that of defatted soy protein (SP) and soy protein isolate (SPI), which is the original source of BL, using sera from patients with soybean allergy. METHODS: Proteins were extracted from SP, SPI, and BL using PBS. Proteins in each sample were analyzed for antigenicity using inhibition ELISA with SP-specific IgE (sIgE), SDS-PAGE, and immunoblotting. Sere from patients with soybean allergy confirmed by an oral food challenge (OFC) (n=6, OFC+ Pt), and from patients who were positive for soy-sIgE without symptoms ( n = 7, sIgE+ Pt) were used for these assays. The cross-antigenicity of SP and BL with cow's milk (CM) proteins was also analyzed in the sera from patients with CM allergy using inhibition ELISA. RESULTS: SDS-PAGE showed that the proteins in BL produced a smear-like band in the low-molecular-weight region compared with that in SP and SPI. Inhibition ELISA against SP-sIgE showed that BL had a significantly lower inhibition rate than that of SP in both OFC+ Pt and sIgE+ Pt. Immunoblotting analysis showed that the bands of BL were thinner than those of SP and SPI. Additionally, SP and BL showed no cross-antigenicity with CM proteins. CONCLUSION: The proteins in BL was partially digested, and its antigenicity was lower than that of SP and SPI.
Assuntos
Hipersensibilidade a Leite , Proteínas de Soja , Animais , Bovinos , Feminino , Fórmulas Infantis , Cetonas , MagrezaRESUMO
BACKGROUND: We previously reported that the specific IgE levels to αs1-casein (CN) and ß-CN in patients with cow's milk allergy decreased with similar dynamics during oral immunotherapy. Therefore, we hypothesized that αs1- and ß-CN have strong cross-reactivity among CN components, despite the low similarity in the full-length amino acid sequences. METHODS: The αs1-, ß-, and κ-CN were purified from commercial cow's milk. We recruited 39 patients with cow's milk allergy, and the serum IgE levels for each CN component were measured by enzyme-linked immunosorbent assay (ELISA). Cross-reactivity between CN components was investigated by competitive ELISA against αs1-CN. Sequence homology between CN components at the peptide level was calculated using in silico analysis and quantified by the property distance (PD) value. RESULTS: The αs1-CN-specific IgE levels exhibited a strong positive correlation with the ß-CN-specific IgE (r = 0.945, P < .001). Complete competition was observed by ß-CN against αs1-CN, suggesting the presence of common epitopes between them. In silico analysis detected 24 peptide sets with PD values lower than 10 between αs1- and ß-CN, and 14 sets between αs1- and κ-CN. The amino acid sequences of αs1-CN (E61-E70) and ß-CN (I12-E21) that showed the lowest PD value (5.30) were present in the characteristic sequence known as casein phosphopeptide (CPP). CONCLUSION: We detected strong cross-reactivity between CN components. Furthermore, we found highly homologous sequences in the CPP region, which contains a core sequence of "SSSEE" with phosphorylated serine residues.
Assuntos
Caseínas , Hipersensibilidade a Leite , Animais , Bovinos , Simulação por Computador , Feminino , Humanos , Imunoglobulina E , LeiteRESUMO
BACKGROUND: The effect of oral immunotherapy (OIT) on wheat allergy is promising in terms of the potential to obtain desensitization; however, the frequency of exercise-induced allergic reactions on desensitization (EIARDs) and the associated risk factors remain to be determined. METHODS: Twenty-five patients underwent rush OIT for wheat allergy, and 21 achieved the full-dose intake of wheat products (5 g of wheat protein). Exercise-provocation tests were repeatedly performed after the ingestion of a full-dose wheat product. The time-course of the levels of the specific IgEs (sIgE) to wheat extract, total gliadin, deamidated gliadin, recombinant gliadin components (α/ß-, γ- and ω-5-), and glutenin (high and low molecular weight) components was analyzed using ImmunoCAP® , ELISA, or IgE immunoblotting. RESULTS: Fourteen patients (66.7%) were diagnosed as EIARD+, which remained 5 years after rush OIT in 11 patients (52.4%). There were no differences in the clinical backgrounds of the EIARD+ and EIARD- patients. However, EIARD+ patients showed significantly higher sIgE levels to all gliadin and glutenin components than EIARD- patients before OIT. The sIgE levels to each component decreased equally after 1 and 2 years of OIT. On IgE immunoblotting, sera from all patients reacted to the multiple gluten bands, and some reacted to the water-soluble bands. The intensity of all IgE-reactive bands also became equally lighter after OIT. CONCLUSIONS: EIARDs were frequently observed and remained for a long period after successful OIT for wheat allergy. None of the specific wheat components were found to contribute to EIARDs.
Assuntos
Exercício Físico , Imunoglobulina E , Imunoterapia , Hipersensibilidade a Trigo , Alérgenos , Dessensibilização Imunológica , Gliadina , Humanos , Hipersensibilidade a Trigo/diagnóstico , Hipersensibilidade a Trigo/terapiaAssuntos
Caseínas , Fosfopeptídeos , Humanos , Animais , Fosfosserina , Leite , Imunoglobulina E/metabolismoAssuntos
Caseínas , Hipersensibilidade a Leite , Animais , Caseínas/efeitos adversos , Bovinos , Feminino , Humanos , Imunoglobulina E , Lactente , Proteínas do Leite , Fosfopeptídeos , Testes CutâneosAssuntos
Caseínas/imunologia , Dessensibilização Imunológica/métodos , Isotipos de Imunoglobulinas/sangue , Hipersensibilidade a Leite/terapia , Administração Oral , Animais , Criança , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Isotipos de Imunoglobulinas/imunologia , Masculino , Leite/imunologia , Hipersensibilidade a Leite/sangue , Hipersensibilidade a Leite/imunologiaRESUMO
INTRODUCTION: Immune response to cow's milk allergen (CMA) has been analyzed mostly using crude milk antigen or a mixture of various caseins. This study aimed to assess the changes in the immunological response against αS1-casein during oral immunotherapy (OIT) and to investigate the mechanism of tolerance. METHODS: We have performed rush OIT to 39 patients with CMA and obtained the serum samples up to 3 years after OIT. Immunoglobulin E (IgE) and IgG4 antibodies specific to highly purified αS1-casein as well as passively-sensitized basophil activation were evaluated using the serial samples. Furthermore, we examined whether basophil activation led by the pre-OIT serum was suppressed by the post-OIT serum, or by the tolerant serum obtained from naturally outgrown patients. RESULTS: Specific IgE to αS1-casein was significantly reduced after OIT. Specific IgG4 (sIgG4) to αS1-casein was also detected in most of the pre-OIT sera, which was not significantly increased after OIT. Activation of passively-sensitized basophils to αS1-casein was significantly reduced after 2 years (14% ± 19%) and 3 years (19% ± 18%) post-OIT compared with pre-OIT (%CD63high basophils; 51% ± 27%). Furthermore, the addition of post-OIT or tolerant serum to pre-OIT serum significantly suppressed the basophil activation. This suppression was abrogated by washing the supernatant after passive sensitization, but not by depleting IgG antibodies from post-OIT or tolerant sera, nor by blocking FcγRIIb using an anti-FcγR antibody. CONCLUSIONS: αS1-casein-sIgG4 plays a minor role in tolerance mechanisms in cases of CMA; humoral factors other than antigen-sIgG4 may be involved.