Quantifying habitat loss and modification from recent expansion of energy infrastructure in an isolated, peripheral greater sage-grouse population.

New technologies and increasing energy demand have contributed to rapid expansion of unconventional oil and gas development in the U.S. in the past two decades. Quantifying the effects of energy infrastructure on land cover and wildlife habitat is essential for informing land-use policy, developing wildlife conservation strategies, and projecting impacts of future development. The greater sage-grouse (Centrocercus urophasianus; GrSG) is a species of concern in sagebrush ecosystems of the western U.S. and Canada and the focus of widespread conservation and management efforts. Increasing energy development within GrSG range has prompted the need to quantify and predict impacts of energy infrastructure on their habitat and populations. We mapped the annual distribution, surface type, and activity level of energy and non-energy infrastructure in the Parachute-Piceance-Roan (PPR), a small, peripheral greater sage-grouse population in Colorado with expanding oil and gas development, from 2005 to 2015. During that time, the footprint of energy infrastructure more than doubled to 3,275 ha (+108.6%), including 195 new well pads, 930 ha of new pipelines, and 230 km of new roads. In contrast, non-energy infrastructure decreased to 532 ha (-8.3%). The majority of energy infrastructure present each year (77-84%) was supporting infrastructure (i.e. facilities, roads, pipelines) rather than well pads, with an average of 2.24 ± 0.52 SE ha of supporting infrastructure per ha of well pad. Pipelines comprised 74-80% of reclaimed surface and roads comprised 54-69% of disturbed surface across years. By 2015, anthropogenic infrastructure covered 2.70% of occupied range and 2.93% of GrSG habitat, and energy infrastructure covered 2.50% and 10.79% of two priority habitat management area zones in the PPR. Three land cover classes most affected by energy infrastructure were also those strongly selected by GrSG. Topographic constraints appear to concentrate energy infrastructure in areas with gentler topography that also have the highest GrSG use. Together, these patterns suggest that future energy development will cause substantial additional loss and modification of GrSG habitat in the PPR. Our findings are valuable for assessing surface disturbance caps for land-use management and projections of energy infrastructure effects on wildlife habitat in this and other expanding oil and gas fields.

Sampling blood from big brown bats (Eptesicus fuscus) in the field with and without anesthesia: impacts on survival.

Blood was collected from wild big brown bats (Eptesicus fuscus) with and without anesthesia in Fort Collins, Colorado in 2004 to assess the impacts of these procedures on short-term survival and 1-yr return rates. Short-term survival and 1-yr return rates after release were passively monitored using PIT tag detection hoops placed at selected buildings. Comparison of 14-day maximum likelihood survival estimates from bats not bled (142 adult females, 62 volant juveniles), and bats sampled for blood with anesthesia (96 adult females, 23 volant juveniles) and without anesthesia (112 adult females, 22 volant juveniles) indicated no adverse effects of either treatment (juveniles: chi(2) = 53.38, df = 41, P = 0.09; adults: chi(2) = 39.09, df = 44, P = 0.68). Return rates of bats one year after sampling were similar among adult female controls (75.4%, n = 142, 95% CI = 67.4-82.2%), females sampled for blood with anesthesia (83.0%, n = 112, 95% CI = 74.8-89.5%), and females sampled without anesthesia (87.5%, n = 96, 95% CI = 79.2-93.4%). Lack of an effect was also noted in 1-yr return rates of juvenile females. These data suggest that the use of anesthesia during sampling of blood has no advantages in terms of enhancement of survival in big brown bats.

Prevalence of neutralizing antibodies to rabies virus in serum of seven species of insectivorous bats from Colorado and New Mexico, United States.

We determined the presence of rabies-virus-neutralizing antibodies (RVNA) in serum of 721 insectivorous bats of seven species captured, sampled, and released in Colorado and New Mexico, United States in 2003-2005. A subsample of 160 bats was tested for rabies-virus RNA in saliva. We sampled little brown bats (Myotis lucifugus) at two maternity roosts in Larimer County, Colorado; big brown bats (Eptesicus fuscus) at three maternity roosts in Morgan County, Colorado; and big brown bats at five maternity roosts in Larimer County. We also sampled hoary bats (Lasiurus cinereus) and silver-haired bats (Lasionycteris noctivagans) captured while drinking or foraging over water in Bernalillo County, New Mexico and at various locations in Larimer County. Big brown bats, little brown bats, long-legged myotis (Myotis volans), long-eared myotis (Myotis evotis), and fringed myotis (Myotis thysanodes) were also sampled over water in Larimer County. All species except long-eared myotis included individuals with RVNA, with prevalences ranging from 7% in adult female silver-haired bats to 32% in adult female hoary bats. None of the bats had detectable rabies-virus RNA in oropharyngeal swabs, including 51 bats of 5 species that had RVNA in serum. Antibody-positive bats were present in nine of the 10 maternity colonies sampled. These data suggest that wild bats are commonly exposed to rabies virus and develop a humoral immune response suggesting some degree of viral replication, but many infections fail to progress to clinical disease.

Development of 10 polymorphic microsatellite loci isolated from the mountain beaver, Aplodontia rufa rufa (Rafinesque).

We developed 10 microsatellite markers for the mountain beaver, Aplodontia rufa rufa. In three populations of A. r. rufa, the number of alleles for these loci ranged from monomorphic to nine. Average observed heterozygosities in these populations ranged from 0.29 to 0.60. We also tested previously published markers from the endangered subspecies A. r. nigra in A. r. rufa populations.

Landscape genetics of raccoons (Procyon lotor) associated with ridges and valleys of Pennsylvania: implications for oral rabies vaccination programs.

Raccoons are the reservoir for the raccoon rabies virus variant in the United States. To combat this threat, oral rabies vaccination (ORV) programs are conducted in many eastern states. To aid in these efforts, the genetic structure of raccoons (Procyon lotor) was assessed in southwestern Pennsylvania to determine if select geographic features (i.e., ridges and valleys) serve as corridors or hindrances to raccoon gene flow (e.g., movement) and, therefore, rabies virus trafficking in this physiographic region. Raccoon DNA samples (n = 185) were collected from one ridge site and two adjacent valleys in southwestern Pennsylvania (Westmoreland, Cambria, Fayette, and Somerset counties). Raccoon genetic structure within and among these study sites was characterized at nine microsatellite loci. Results indicated that there was little population subdivision among any sites sampled. Furthermore, analyses using a model-based clustering approach indicated one essentially panmictic population was present among all the raccoons sampled over a reasonably broad geographic area (e.g., sites up to 36 km apart). However, a signature of isolation by distance was detected, suggesting that widths of ORV zones are critical for success. Combined, these data indicate that geographic features within this landscape influence raccoon gene flow only to a limited extent, suggesting that ridges of this physiographic system will not provide substantial long-term natural barriers to rabies virus trafficking. These results may be of value for future ORV efforts in Pennsylvania and other eastern states with similar landscapes.