RESUMO
Porcine circovirus type 3 (PCV3) was first detected in 2016 and has been reported in many pig-producing countries around the world, including Vietnam. PCV3 has been found in complex cases with multiple clinical syndromes in swine. In this study, we investigated the genetic diversity of PCV3 strains circulating in Vietnam. A total of 249 samples were collected from swine farms located in eight provinces of Vietnam, and 11.65% (29/249) of these samples were found to contain PCV3. The ORF2 genes from the 29 PCV3-positive samples were amplified, purified, and sequenced. Phylogenetic analysis showed that 23 of these strains belonged to the PCV3b subtype, while the remaining six strains belonged to subtype c and subtype a (a-1 and a-2). Analysis of the ORF2 genes indicated that the 29 PCV3 strains had high sequence identity (96.90-100% at the genomic level and 96.19-100% at the amino acid level). Fifteen amino acid substitutions were found in predicted B-cell epitopes in the capsid proteins of the Vietnamese PCV3 strains.
Assuntos
Infecções por Circoviridae , Circovirus , Doenças dos Suínos , Suínos , Animais , Proteínas do Capsídeo/genética , Circovirus/genética , Filogenia , Vietnã/epidemiologia , Infecções por Circoviridae/veterinária , Doenças dos Suínos/epidemiologia , Variação GenéticaRESUMO
African swine fever virus (ASF) has circulated in Vietnam since 2018, causing significant losses to the pig industry. Quick, accurate diagnosis of African swine fever virus (ASFV) infection is crucial for controlling the disease. The detection of the virus in piglets with congenital tremors is described in this paper. ASFV was detected in brain tissues by polymerase chain reaction (PCR) and immunohistochemistry. Classical swine fever virus, porcine parvovirus, porcine reproductive and respiratory syndrome virus, and pseudorabies virus were not detected by PCR, suggesting that the ASFV was the cause of these neurological signs.
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Vírus da Febre Suína Africana , Febre Suína Africana , Vírus da Febre Suína Clássica , Vírus da Síndrome Respiratória e Reprodutiva Suína , Febre Suína Africana/diagnóstico , Vírus da Febre Suína Africana/genética , Animais , Suínos , TremorRESUMO
MAIN CONCLUSION: Riboside type cytokinins are key components in cytokinin metabolism, transport, and sensitivity, making them important functional signals in plant growth and development and environmental stress responses. Cytokinin (CKs) are phytohormones that regulate multiple processes in plants and are critical for agronomy, as they are involved in seed filling and plant responses to biotic and abiotic stress. Among the over 30 identified CKs, there is uncertainty about the roles of many of the individual CK structural forms. Cytokinin free bases (CKFBs), have been studied in great detail, but, by comparison, roles of riboside-type CKs (CKRs) in CK metabolism and associated signaling pathways and their distal impacts on plant physiology remain largely unknown. Here, recent findings on CKR abundance, transport and localization, are summarized, and their importance in planta is discussed. The history of CKR analyses is reviewed, in the context of the determination of CK metabolic pathways, and research on CKR affinity for CK receptors, all of which yield essential insights into their functions. Recent studies suggest that CKR forms are a lot more than a group of transport CKs and, beyond this, they play important roles in plant development and responses to environmental stress. In this context, this review discusses the involvement of CKRs in plant development, and highlight the less anticipated functions of CKRs in abiotic stress tolerance. Based on this, possible mechanisms for CKR modes of action are proposed and experimental approaches to further uncover their roles and future biotechnological applications are suggested.
Assuntos
Citocininas , Reguladores de Crescimento de Plantas , Desenvolvimento Vegetal , Plantas , Estresse FisiológicoRESUMO
Isopentenyltransferase (IPT) in plants regulates a rate-limiting step of cytokinin (CTK) biosynthesis. IPTs are recognized as key regulators of CTK homeostasis and phytohormone crosstalk in both biotic and abiotic stress responses. Recent research has revealed the regulatory function of IPTs in gene expression and metabolite profiles including source-sink modifications, energy metabolism, nutrient allocation and storage, stress defence and signalling pathways, protein synthesis and transport, and membrane transport. This suggests that IPTs play a crucial role in plant growth and adaptation. In planta studies of IPT-driven modifications indicate that, at a physiological level, IPTs improve stay-green characteristics, delay senescence, reduce stress-induced oxidative damage and protect photosynthetic machinery. Subsequently, these improvements often manifest as enhanced or stabilized crop yields and this is especially apparent under environmental stress. These mechanisms merit consideration of the IPTs as 'master regulators' of core cellular metabolic pathways, thus adjusting plant homeostasis/adaptive responses to altered environmental stresses, to maximize yield potential. If their expression can be adequately controlled, both spatially and temporally, IPTs can be a key driver for seed yield. In this review, we give a comprehensive overview of recent findings on how IPTs influence plant stress physiology and yield, and we highlight areas for future research.
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Alquil e Aril Transferases , Citocininas , Adaptação Fisiológica/genética , Alquil e Aril Transferases/genética , Reguladores de Crescimento de Plantas , Estresse Fisiológico/genéticaRESUMO
MAIN CONCLUSION: Early cytokinin activity and late abscisic acid dynamics during wheat kernel development correspond to cultivars with higher yield potential. Cytokinins represent prime targets for marker development for wheat breeding programs. Two major phytohormone groups, abscisic acid (ABA) and cytokinins (CKs), are of crucial importance for seed development. Wheat (Triticum aestivum L.) yield is, to a high degree, determined during the milk and dough stages of kernel development. Therefore, understanding the hormonal regulation of these early growth stages is fundamental for crop-improvement programs of this important cereal. Here, we profiled ABA and 25 CK metabolites (including active forms, precursors and inactive conjugates) during kernel development in five field-grown wheat cultivars. The levels of ABA and profiles of CK forms varied greatly among the tested cultivars and kernel stages suggesting that several types of CK metabolites are involved in spatiotemporal regulation of kernel development. The seed yield potential was associated with the elevated levels of active CK levels (tZ, cZ). Interestingly, the increased kernel cZ levels were followed by higher ABA production, suggesting there is an interaction between these two phytohormones. Furthermore, we analyzed the expression patterns of representatives of the four main CK metabolic gene families. The unique transcriptional patterns of the IPT (biosynthesis) and ZOG (reversible inactivation) gene family members (GFMs) in the high and low yield cultivars additionally indicate that there is a significant association between CK metabolism and yield potential in wheat. Based on these results, we suggest that both CK metabolites and their associated genes, can serve as important, early markers of yield performance in modern wheat breeding programs.
Assuntos
Ácido Abscísico , Citocininas , Sementes , Triticum , Ácido Abscísico/metabolismo , Produção Agrícola , Citocininas/metabolismo , Melhoramento Vegetal , Reguladores de Crescimento de Plantas , Sementes/crescimento & desenvolvimento , Sementes/metabolismo , Triticum/genética , Triticum/crescimento & desenvolvimentoRESUMO
PURPOSE: This study aimed to investigate the diversity of Balantioides coli (B. coli) based on the 18S rDNA of isolates collected from weaned pigs with diarrhea and then select a B. coli isolate to determine its pathogenicity in weaned pigs through experimental infection. METHODS: The genetic analysis of field-isolated B. coli strains was based on sequencing of the partial 18S rDNA genetic fragment and the pathogenicity of an isolate of B. coli (DN2018-2-DTD) was identified through an experimental infection. RESULTS: The fourteen B. coli isolates shared 98.47-100% nucleotide similarity and were divided into two clades in a phylogenetic tree. In an infection experiment, a slight increase in the body temperature was identified in two infected groups as compared to that in the control group (P < 0.05) from days post-infection (dpi) 12-18 and from dpi 20-27. In the group infected with 3000 CPG/TPG, 2/9 pigs started to develop diarrhea on day two. In the group infected with 500 CPG/TPG, 8/9 pigs had diarrhea on dpi 3. Trophozoites/cysts of B. coli were firstly detected in feces on dpi 3 in a group of pigs infected with a dose of 3000 CPG/TPG, and CPG/TPG reached its peak at dpi 5 in both groups of infected pigs (35,450 and 13,250 CPG/TPG). There was a significant difference in the bodyweight of the control group (noninfected) as compared to that of the groups of infected pigs on dpi 7 (P < 0.05), 14 (P < 0.001) and 28 (P < 0.01). Lesions were solely recorded in infected pigs, including white ulcerative nodules with necrosis scattered in the mucosa of the colon and cecum on dpi 7, 14, and 28. CONCLUSION: This is the first study to investigate the pathogenicity of B. coli in Vietnamese pigs to demonstrate that B. coli could induce necrotic ulcerative enteritis and diarrhea in weaned pigs.
Assuntos
Doenças dos Suínos , Animais , Povo Asiático , Diarreia/veterinária , Fezes , Humanos , Filogenia , Suínos , Virulência , DesmameRESUMO
Cytokinins (CKs) play a fundamental role in regulating dynamics of organ source/sink relationships during plant development, including flowering and seed formation stages. As a result, CKs are key drivers of seed yield. The cytokinin oxidase/dehydrogenase (CKX) is one of the critical enzymes responsible for regulating plant CK levels by causing their irreversible degradation. Variation of CKX activity is significantly correlated with seed yield in many crop species while in soybean (Glycine max L.), the possible associations between CKX gene family members (GFMs) and yield parameters have not yet been assessed. In this study, 17 GmCKX GFMs were identified, and natural variations among GmCKX genes were probed among soybean cultivars with varying yield characteristics. The key CKX genes responsible for regulating CK content during seed filling stages of reproductive development were highlighted using comparative phylogenetics, gene expression analysis and CK metabolite profiling. Five of the seventeen identified GmCKX GFMs, showed natural variations in the form of single nucleotide polymorphisms (SNPs). The gene GmCKX7-1, with high expression during critical seed filling stages, was found to have a non-synonymous mutation (H105Q), on one of the active site residues, Histidine 105, previously reported to be essential for co-factor binding to maintain structural integrity of the enzyme. Soybean lines with this mutation had higher CK content and desired yield characteristics. The potential for marker-assisted selection based on the identified natural variation within GmCKX7-1, is discussed in the context of hormonal control that can result in higher soybean yield.