Body temperature is a repeatable trait in a free-ranging passerine bird.

Body temperature (Tb) affects animal function through its influence on rates of biochemical and biophysical reactions, the molecular structures of proteins and tissues, and, ultimately, organismal performance. Despite its importance in driving physiological processes, there are few data on how much variation in Tb exists within populations of organisms, and whether this variation consistently differs among individuals over time (i.e. repeatability of a trait). Here, using thermal radio-frequency identification implants, we quantified the repeatability of Tb, both in the context of a fixed average environment (∼21°C) and across ambient temperatures (6-31°C), in a free-living population of tree swallows (Tachycineta bicolor, n=16). By experimentally trimming the ventral plumage of a subset of female swallows (n=8), we also asked whether the repeatability of Tb is influenced by the capacity to dissipate body heat. We found that both female and male tree swallow Tb was repeatable at 21°C (R=0.89-92), but female Tb was less repeatable than male Tb across ambient temperature (Rfemale=0.10, Rmale=0.58), which may be due to differences in parental investment. Trimmed birds had on average lower Tb than control birds (by ∼0.5°C), but the repeatability of female Tb did not differ as a function of heat dissipation capacity. This suggests that trimmed individuals adjusted their Tb to account for the effects of heat loss on Tb. Our study provides a first critical step toward understanding whether Tb is responsive to natural selection, and for predicting how animal populations will respond to climatic warming.

Heat dissipation capacity influences reproductive performance in an aerial insectivore.

Climatic warming is predicted to increase the frequency of extreme weather events, which may reduce an individual's capacity for sustained activity because of thermal limits. We tested whether the risk of overheating may limit parental provisioning of an aerial insectivorous bird in population decline. For many seasonally breeding birds, parents are thought to operate close to an energetic ceiling during the 2-3 week chick-rearing period. The factors determining the ceiling remain unknown, although it may be set by an individual's capacity to dissipate body heat (the heat dissipation limitation hypothesis). Over two breeding seasons we experimentally trimmed the ventral feathers of female tree swallows (Tachycineta bicolor) to provide a thermal window. We then monitored maternal and paternal provisioning rates, nestling growth rates and fledging success. We found the effect of our experimental treatment was context dependent. Females with an enhanced capacity to dissipate heat fed their nestlings at higher rates than controls when conditions were hot, but the reverse was true under cool conditions. Control females and their mates both reduced foraging under hot conditions. In contrast, male partners of trimmed females maintained a constant feeding rate across temperatures, suggesting attempts to match the feeding rate of their partners. On average, nestlings of trimmed females were heavier than controls, but did not have a higher probability of fledging. We suggest that removal of a thermal constraint allowed females to increase provisioning rates, but additionally provided nestlings with a thermal advantage via increased heat transfer during maternal brooding. Our data provide support for the heat dissipation limitation hypothesis and suggest that depending on temperature, heat dissipation capacity can influence reproductive success in aerial insectivores.

Ambient temperature preferences of chimney swifts (Chaetura pelagica) for Nest Site Selection.

Chimney swift (Chaetura pelagica) populations are declining rapidly, with no clear indication as to why. Reduced availability of nesting habitat (chimneys) was thought to be a limiting factor for this threatened species, but data from Ontario, Canada did not support this hypothesis. If availability is not limiting, then perhaps habitat quality may play a role. We examined the thermal aspect of chimneys that are used by nesting swifts. To do so, we identified upper and lower thermal limits influencing the selection of chimneys for nesting. Across Nova Scotia and New Brunswick, we deployed temperature loggers in 19 chimneys, 11 of which contained chimney swift nests and 8 that were deemed suitable for nesting but were not occupied. Temperature readings were recorded at 30-min intervals from 23 June to 15 September 2017. We found that chimney occupancy by swifts was negatively correlated with maximum and mean chimney temperature. We did not find a relationship between occupancy and minimum temperature, temperature fluctuations, or chimney material. These results indicate that chimney swifts are preferentially selecting cooler chimneys for nesting.

Population trends of grassland birds in North America are linked to the prevalence of an agricultural epizootic in Europe.

Globalization of trade has dramatic socioeconomic effects, and, intuitively, significant ecological effects should follow. However, few quantitative examples exist of the interrelationship of globalization, socioeconomics, and ecological patterns. We present a striking illustration of a cascade in which bovine spongiform encephalopathy (BSE; "mad cow disease") outbreaks in Europe exerted pressure on global beef markets, subsequently affecting North American hayfields and grassland bird populations. We examined competing models, which linked the prevalence of BSE in five focal countries, volume of beef exports to those countries from North America, and the amount of hayfield harvested and the abundance of grassland birds in North America. We found that (i) imports from North America increased 1 y after BSE outbreaks; (ii) probably because fewer cattle remained, the hay harvest in North America was reduced 2 y after the outbreak; (iii) the reduced hay harvest yielded a positive response in grassland bird populations 3 y after the outbreak.

Historical pesticide applications coincided with an altered diet of aerially foraging insectivorous chimney swifts.

Numerous environmental pressures have precipitated long-term population reductions of many insect species. Population declines in aerially foraging insectivorous birds have also been detected, but the cause remains unknown partly because of a dearth of long-term monitoring data on avian diets. Chimney swifts (Chaetura pelagica) are a model aerial insectivore to fill such information gaps because their roosting behaviour makes them easy to sample in large numbers over long time periods. We report a 48-year-long (1944-1992) dietary record for the chimney swift, determined from a well-preserved deposit of guano and egested insect remains in Ontario (Canada). This unique archive of palaeo-environmental data reflecting past chimney swift diets revealed a steep rise in dichlorodiphenyltrichloroethane (DDT) and metabolites, which were correlated with a decrease in Coleoptera remains and an increase in Hemiptera remains, indicating a significant change in chimney swift prey. We argue that DDT applications decimated Coleoptera populations and dramatically altered insect community structure by the 1960s, triggering nutritional consequences for swifts and other aerial insectivores.

Forest degradation drives widespread avian habitat and population declines.

In many regions of the world, forest management has reduced old forest and simplified forest structure and composition. We hypothesized that such forest degradation has resulted in long-term habitat loss for forest-associated bird species of eastern Canada (130,017 km2) which, in turn, has caused bird-population declines. Despite little change in overall forest cover, we found substantial reductions in old forest as a result of frequent clear-cutting and a broad-scale transformation to intensified forestry. Back-cast species distribution models revealed that breeding habitat loss occurred for 66% of the 54 most common species from 1985 to 2020 and was strongly associated with reduction in old age classes. Using a long-term, independent dataset, we found that habitat amount predicted population size for 94% of species, and habitat loss was associated with population declines for old-forest species. Forest degradation may therefore be a primary cause of biodiversity decline in managed forest landscapes.

Mitochondrial evidence for panmixia despite perceived barriers to gene flow in a widely distributed waterbird.

We examined the mitochondrial genetic structure of American white pelicans (Pelecanus erythrorhynchos) to: 1) verify or refute whether American white pelicans are panmictic and 2) understand if any lack of genetic structure is the result of contemporary processes or historical phenomena. Sequence analysis of mitochondrial DNA control region haplotypes of 367 individuals from 19 colonies located across their North American range revealed a lack of population genetic or phylogeographic structure. This lack of structure was unexpected because: 1) Major geographic barriers such as the North American Continental Divide are thought to limit dispersal; 2) Differences in migratory behavior are expected to promote population differentiation; and 3) Many widespread North American migratory bird species show historic patterns of differentiation resulting from having inhabited multiple glacial refugia. Further, high haplotype diversity and many rare haplotypes are maintained across the species' distribution, despite frequent local extinctions and recolonizations that are expected to decrease diversity. Our findings suggest that American white pelicans have a high effective population size and low natal philopatry. We suggest that the rangewide panmixia we observed in American white pelicans is due to high historical and contemporary gene flow, enabled by high mobility and a lack of effective physical or behavioral barriers.

Roost sites of chimney swift (Chaetura pelagica) form large-scale spatial networks.

Several biodiversity-centered metrics exist to quantify the importance of landscape and habitat features for conservation efforts. However, for species whose habitat use is not quantified by these metrics, such as those in urban areas, we need a method to best identify features for targeted conservation efforts. We investigated the use of social network analysis (SNA) to identify and quantify these critical habitat features. We used SNA to identify network existence in chimney swift (Chaetura pelagica) roost usage, quantify the importance of each roost site, and evaluate the impact of the loss of key sites. We identified a network consisting of ten chimney swift roosts in southern Nova Scotia, Canada, and found that 76% of swifts used more than one roost throughout the breeding season. We also isolated three key (most connected) roost sites. We evaluated the effect of loss of these key sites on the network by using a Wilcoxon-Pratt signed-rank test and by analyzing the structure of the subsequent network. We found that connections between roosts and the structure of the network were significantly affected by the loss of these key sites. Our results show that SNA is a valuable tool that can identify key sites for targeted conservation efforts for species that may not be included in conservation efforts focused purely on biodiversity.

Experimental evidence that hyperthermia limits offspring provisioning in a temperate-breeding bird.

In many vertebrates, parental care can require long bouts of daily exercise that can span several weeks. Exercise, especially in the heat, raises body temperature, and can lead to hyperthermia. Typical strategies for regulating body temperature during endurance exercise include modifying performance to avoid hyperthermia (anticipatory regulation) and allowing body temperature to rise above normothermic levels for brief periods of time (facultative hyperthermia). Facultative hyperthermia is commonly employed by desert birds to economize on water, but this strategy may also be important for chick-rearing birds to avoid reducing offspring provisioning when thermoregulatory demands are high. In this study, we tested how chick-rearing birds balance their own body temperature against the need to provision dependent offspring. We experimentally increased the heat dissipation capacity of breeding female tree swallows (Tachycineta bicolor) by trimming their ventral feathers and remotely monitored provisioning rates, body temperature and the probability of hyperthermia. Birds with an experimentally increased capacity to dissipate heat (i.e. trimmed treatment) maintained higher feeding rates than controls at high ambient temperatures (greater than or equal to 25°C), while maintaining lower body temperatures. However, at the highest temperatures (greater than or equal to 25°C), trimmed individuals became hyperthermic. These results provide evidence that chick-rearing tree swallows use both anticipatory regulation and facultative hyperthermia during endurance performance. With rising global temperatures, individuals may need to increase their frequency of facultative hyperthermia to maintain nestling provisioning, and thereby maximize reproductive success.

Social information trumps vegetation structure in breeding-site selection by a migrant songbird.

To maximize fitness, organisms must assess and select suitable habitat. Early research studying birds suggested that organisms consider primarily vegetation structural cues in their habitat choices. We show that experimental exposure to singing in the post-breeding period provides a social cue that is used for habitat selection the following year by a migrant songbird, the black-throated blue warbler (Dendroica caerulescens). Our experimental social cues coerced individuals to adopt territories in areas of very poor habitat quality where individuals typically do not occur. This indicates that social information can override typical associations with vegetation structure. We demonstrate that a strong settlement response was elicited because post-breeding song at a site is highly correlated with reproductive success. These results constitute a previously undocumented, but highly parsimonious mechanism for the inadvertent transfer of reproductive (public) information from successful breeders to dispersers. We hypothesize that post-breeding song is a pervasive and reliable cue for species that communicate vocally, inhabit temporally autocorrelated environments, produce young asynchronously and/or abandon territories after reproductive failure.

Nightjars may adjust breeding phenology to compensate for mismatches between moths and moonlight.

Phenology match-mismatch usually refers to the extent of an organism's ability to match reproduction with peaks in food availability, but when mismatch occurs, it may indicate a response to another selective pressure. We assess the value of matching reproductive timing to multiple selective pressures for a migratory lunarphilic aerial insectivore bird, the whip-poor-will (Antrostomus vociferus). We hypothesize that a whip-poor-will's response to shifts in local phenology may be constrained by long annual migrations and a foraging mode that is dependent on both benign weather and the availability of moonlight. To test this, we monitored daily nest survival and overall reproductive success relative to food availability and moon phase in the northern part of whip-poor-will's breeding range. We found that moth abundance, and potentially temperature and moonlight, may all have a positive influence on daily chick survival rates and that the lowest chick survival rates for the period between hatching and fledging occurred when hatch was mismatched with both moths and moonlight. However, rather than breeding too late for peak moth abundance, the average first brood hatch date actually preceded the peak moth abundance and occurred during a period with slightly higher available moonlight than the period of peak food abundance. As a result, a low individual survival rate was partially compensated for by initiating more nesting attempts. This suggests that nightjars were able to adjust their breeding phenology in such a way that the costs of mismatch with food supply were at least partially balanced by a longer breeding season.

THE OCCURRENCE OF PATHOGENS IN AN ENDANGERED POPULATION OF AMERICAN BADGERS (TAXIDEA TAXUS JACKSONI) IN ONTARIO, CANADA.

American badgers ( Taxidea taxus jacksoni) at the periphery of the species' range in Ontario, Canada, are listed as endangered because of an estimated population size of <200 mature individuals. The main threats faced by this population include habitat loss and road mortality. However, on 18 November 2013, a radio-implanted badger was found nonresponsive in an agricultural field with signs consistent with canine distemper virus infection, which was subsequently confirmed. This prompted our investigation into the occurrence of pathogens in this endangered carnivore to better quantify the level of risk infectious disease poses to population persistence. We examined serum samples from nine live-trapped individuals and 27 whole badger specimens submitted for postmortem examination. We found evidence of exposure to canine distemper virus, canine parvovirus, and leptospires. However, infection associated with disease was not the leading cause of mortality. Future research into the effects of disease on kit survival and a comprehensive understanding of disease severity and spread from reservoir populations (e.g., raccoons [ Procyon lotor ] and striped skunks [ Mephitis mephitis ]) to badgers will be of particular importance to the conservation of this endangered population.

Inadvertent social information in breeding site selection of natal dispersing birds.

Several species use the number of young produced as public information (PI) to assess breeding site quality. PI is inaccessible for synchronously breeding birds because nests are empty by the time the young can collect this information. We investigate if location cues are the next best source of inadvertent social information (ISI) used by young prospectors during breeding site choice. We experimentally deployed ISI as decoys and song playbacks of breeding males in suitable and sub-optimal habitats during pre- and post-breeding periods, and monitored territory establishment during the subsequent breeding season for a social, bobolink (Dolichonyx oryzivorus), and a more solitary species, Nelson's sharp-tailed sparrow (Ammodramus nelsoni). The sparrows did not respond to treatments, but bobolinks responded strongly to post-breeding location cues, irrespective of habitat quality. The following year, 17/20 sub-optimal plots to which bobolink males were recruited were defended for at least two weeks, indicating that song heard the previous year could exert a "carry-over attraction" effect on conspecifics the following year. Sixteen recruited males were natal dispersers, as expected when animals have little opportunity to directly sample their natal habitat quality. We suggest that differences in breeding synchronicity may induce an equivalent clinal distribution of ISI use.

Linking Isotopes and Panmixia: High Within-Colony Variation in Feather δ2H, δ13C, and δ15N across the Range of the American White Pelican.

Complete panmixia across the entire range of a species is a relatively rare phenomenon; however, this pattern may be found in species that have limited philopatry and frequent dispersal. American white pelicans (Pelecanus erythrorhyncos) provide a unique opportunity to examine the role of long-distance dispersal in facilitating gene flow in a species recently reported as panmictic across its broad breeding range. This species is also undergoing a range expansion, with new colonies arising hundreds of kilometers outside previous range boundaries. In this study, we use a multiple stable isotope (δ2H, δ13C, δ15N) approach to examine feather isotopic structuring at 19 pelican colonies across North America, with the goal of establishing an isotopic basemap that could be used for assigning individuals at newly established breeding sites to source colonies. Within-colony isotopic variation was extremely high, exceeding 100‰ in δ2H within some colonies (with relatively high variation also observed for δ13C and δ15N). The high degree of within-site variation greatly limited the utility of assignment-based approaches (42% cross-validation success rate; range: 0-90% success). Furthermore, clustering algorithms identified four likely isotopic clusters; however, those clusters were generally unrelated to geographic location. Taken together, the high degree of within-site isotopic variation and lack of geographically-defined isotopic clusters preclude the establishment of an isotopic basemap for American white pelicans, but may indicate that a high incidence of long-distance dispersal is facilitating gene flow, leading to genetic panmixia.

Spatial patterns of immunogenetic and neutral variation underscore the conservation value of small, isolated American badger populations.

Small and isolated populations often exhibit low genetic diversity due to drift and inbreeding, but may simultaneously harbour adaptive variation. We investigate spatial distributions of immunogenetic variation in American badger subspecies (Taxidea taxus), as a proxy for evaluating their evolutionary potential across the northern extent of the species' range. We compared genetic structure of 20 microsatellites and the major histocompatibility complex (MHC DRB exon 2) to evaluate whether small, isolated populations show low adaptive polymorphism relative to large and well-connected populations. Our results suggest that gene flow plays a prominent role in shaping MHC polymorphism across large spatial scales, while the interplay between gene flow and selection was stronger towards the northern peripheries. The similarity of MHC alleles within subspecies relative to their neutral genetic differentiation suggests that adaptive divergence among subspecies can be maintained despite ongoing gene flow along subspecies boundaries. Neutral genetic diversity was low in small relative to large populations, but MHC diversity within individuals was high in small populations. Despite reduced neutral genetic variation, small and isolated populations harbour functional variation that likely contribute to the species evolutionary potential at the northern range. Our findings suggest that conservation approaches should focus on managing adaptive variation across the species range rather than protecting subspecies per se.

Lack of Spatial Immunogenetic Structure among Wolverine (Gulo gulo) Populations Suggestive of Broad Scale Balancing Selection.

Elucidating the adaptive genetic potential of wildlife populations to environmental selective pressures is fundamental for species conservation. Genes of the major histocompatibility complex (MHC) are highly polymorphic, and play a key role in the adaptive immune response against pathogens. MHC polymorphism has been linked to balancing selection or heterogeneous selection promoting local adaptation. However, spatial patterns of MHC polymorphism are also influenced by gene flow and drift. Wolverines are highly vagile, inhabiting varied ecoregions that include boreal forest, taiga, tundra, and high alpine ecosystems. Here, we investigated the immunogenetic variation of wolverines in Canada as a surrogate for identifying local adaptation by contrasting the genetic structure at MHC relative to the structure at 11 neutral microsatellites to account for gene flow and drift. Evidence of historical positive selection was detected at MHC using maximum likelihood codon-based methods. Bayesian and multivariate cluster analyses revealed weaker population genetic differentiation at MHC relative to the increasing microsatellite genetic structure towards the eastern wolverine distribution. Mantel correlations of MHC against geographical distances showed no pattern of isolation by distance (IBD: r = -0.03, p = 0.9), whereas for microsatellites we found a relatively strong and significant IBD (r = 0.54, p = 0.01). Moreover, we found a significant correlation between microsatellite allelic richness and the mean number of MHC alleles, but we did not observe low MHC diversity in small populations. Overall these results suggest that MHC polymorphism has been influenced primarily by balancing selection and to a lesser extent by neutral processes such as genetic drift, with no clear evidence for local adaptation. This study contributes to our understanding of how vulnerable populations of wolverines may respond to selective pressures across their range.

Tracking animal movement by comparing trace element signatures in claws to spatial variability of elements in soils.

Biogeochemical markers in ecology have provided a useful means for indicating geographic origin and movement patterns of species on various temporal and spatial scales. We used trace element analysis to resolve spatial and habitat-specific environmental gradients in elemental distributions that could be used to infer geographic origin and habitat association in a model terrestrial carnivore: American badger (Taxidea taxus jacksoni). To accomplish this, we generated element base-maps using spatial principal component analysis, and assessed habitat-specific signatures using multivariate statistics from soil element concentrations in southwestern Ontario, Canada. Using canonical correlation analysis (CCA) we also test whether element variability in the claw keratin of a terrestrial carnivore could be explained by the chemical variability in the soils of the local environment. Results demonstrated that trace element signatures in soils vary locally with land use practices and soil texture type and broadly with the underlying geology. CCA results suggest that chemical profiles in claws can be linked to the surrounding chemical environment, providing evidence that geographic patterns in mammalian movement can be discerned on the basis of claw chemistry. From this, we conclude that geographic assignment of individuals based on element profiles in their tissues and referenced against soil elemental distributions would be coarse (at a spatial scale of 100-1000 km, depending on the chemical heterogeneity of the landscape), but could be used to assess origin of highly mobile animals or habitat association of individuals. Compared to stable isotope analysis, the assessment of trace elements can provide a much greater level of detail in backcasting animal movement pathways.