Repair of segmental mandibular bone defects in sheep using bone marrow stromal cells and autologous serum scaffold: a pilot study.

AIM: The aim of this study was to evaluate effective bone regeneration using an autologous serum scaffold (alone or seeded with autologous bone marrow-mesenchymal stem cells, BM-MSCs), when implanted in a 30 mm length segmental mandibular defect in sheep. MATERIALS AND METHODS: The bone defect was filled either with serum scaffold alone (control group; n = 5) or combined with BM-MSCs (experimental group; n = 10). Bone regeneration was determined at 12 (T12; 2 control sheep and 4 experimental sheep) and 32 weeks (T32; 3 control and 6 experimental sheep), as measured by computed and microcomputed tomography and histological examination. RESULTS: Two sheep of the Experimental group died after surgery. While complete bone union in the control group was only observed at T32, it was observed both at T12 (1/4 sheep) and T32 (3/4 sheep) in the experimental group. When properties/characteristics of new bone where compared, a better bone quality, similar to native bone, was observed in the scaffold combined with BM-MSCs. CONCLUSIONS: Based on these results, we conclude that the serum scaffold can promote efficient repair of large bone defects, but the combination with BM-MSCs accelerates this process, increasing significantly the amount and quality of bone formed.

3D photogrammetry as a tool for studying erosive processes at a Roman coastal site: the case of the Roman fish-salting plant at Sobreira (Vigo, Spain).

Rising sea levels, along with other biological and human factors, have increased erosion rates at a number of important sites located along the Atlantic coastline. Project GaltFish implemented a series of contingency measures to record some of these sites before they degraded further or totally disappeared. This process involved detailed photogrammetric recording of some of the sites under threat over a set period of time. One of the sites selected for this project was Sobreira (Vigo, Galicia): a Roman fish-salting factory which was partially destroyed by building activity in the 1980s and the remains of which are under threat from marine erosion and human action. In order to study the site, two photogrammetric models were created to examine the effect of erosive processes across the course of one year. The results illustrate that photogrammetry is an efficient tool for recording and analysing the issue of erosion. The data compiled helped in designing additional action in the factory, which was subject to a rescue excavation to record and help protect the site from further damage. This paper presents the results of this project, as well as the methodology used to produce the models, the data generated and their analysis. It is argued that the methodology can be used to collect and analyse data from other sites, and that this data could inform the political/administrative decision-making processes which concern the future management and preservation of archaeological sites under threat.