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Biochem Biophys Res Commun ; 529(4): 898-903, 2020 09 03.
Artigo em Inglês | MEDLINE | ID: mdl-32819596

RESUMO

Clock genes express circadian rhythms in most organs. These rhythms are organized throughout the whole body, regulated by the suprachiasmatic nucleus (SCN) in the brain. Disturbance of these clock gene expression rhythms is a risk factor for diseases such as obesity and cancer. To understand the mechanism of regulating clock gene expression rhythms in vivo, multiple real time recording systems are required. In the present study, we developed a double recording system of Period1 expression rhythm in peripheral tissue (liver) and the brain. In peripheral tissue, quantification of gene expression in a steadily moving target was achieved by using a photomultiplier tube (PMT) attached to a tissue contact optical sensor (TCS). Using this technique, we were able to analyze circadian rhythms of clock gene expression over a prolonged period in the liver and olfactory bub (OB) of the brain. The present double recording system has no effect on behavioral activity or rhythm. Our novel system thus successfully quantifies clock gene expression in deep areas of the body in freely moving mice for a period sufficient to analyze circadian dynamics. In addition, our double recording system can be widely applied to many areas of biomedical research, as well as applications beyond medicine.


Assuntos
Ritmo Circadiano/fisiologia , Transdução de Sinal Luminoso , Fígado/fisiologia , Bulbo Olfatório/fisiologia , Proteínas Circadianas Period/genética , Núcleo Supraquiasmático/fisiologia , Animais , Ritmo Circadiano/efeitos da radiação , Eletrodos Implantados , Regulação da Expressão Gênica , Genes Reporter , Luz , Fígado/efeitos da radiação , Luciferases/genética , Luciferases/metabolismo , Camundongos , Camundongos Transgênicos , Movimento/fisiologia , Bulbo Olfatório/efeitos da radiação , Optogenética , Proteínas Circadianas Period/metabolismo , Técnicas Estereotáxicas , Núcleo Supraquiasmático/efeitos da radiação
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