Community composition of invasive, outbreak, and non-pest snail species along a source spring-to-fishpond gradient in a spatially structured aquacultural region.

Agricultural lands are integrated into and interact with natural areas. Such is the case of Emek HaMa'ayanot, northern Israel, comprising a springs-rich area characterized by multiple land-uses, including spring-water-based aquaculture, recreational springs, and nature reserves. Aquacultural farms suffer from pest snails that carry fish disease; in the study region, these species are invasive (Thiara scabra, Tarebia granifera, Pseudosuccinea columella) and outbreak endemic (Melanoides tuberculata). Previous snail control efforts have focused on individual fishponds without considering management on larger environmental scales in the waterways from the source springs to the fish farms. To broaden our understanding of the status of the pest snail problem in the study area prior to suggesting environmental managerial solutions, we quantified changes in the community composition of snail species along the springs-to-fishponds gradients in a spatially explicit system. We found a remarkable increase in pest snail abundances along these gradients, indicating that pest snails might be invading upstream towards the springs. There were always nearly 100% pest snails in the endpoint sites for water tracks that ended in fishponds. Moreover, pest snails dominated the site when it was used as a fishpond, even though the site was also a spring. In contrast, in a water track that does not end in a fish farm, the relative abundances of non-pest snail species was similar between the source spring and the downstream endpoint, in spite of an increase in pest snail abundance at a midpoint site. These results suggest that invasive pest snails are actively moving upstream and that the fishponds have a marked upstream effect on the ability of non-pest snails to resist pest species invasions. We suggest further investigation of possible strategies for biocontrol of the observed invasion of the snails into natural areas as a basis for environmental management efforts. Finally, the observations made during this study could have practical global implications for snail management in aquaculture and agriculture, and for the control of snails and snail vectors implicated in animal and human diseases.

Mutant C. elegans mitofusin leads to selective removal of mtDNA heteroplasmic deletions across generations to maintain fitness.

BACKGROUND: Mitochondrial DNA (mtDNA) is present at high copy numbers in animal cells, and though characterized by a single haplotype in each individual due to maternal germline inheritance, deleterious mutations and intact mtDNA molecules frequently co-exist (heteroplasmy). A number of factors, such as replicative segregation, mitochondrial bottlenecks, and selection, may modulate the exitance of heteroplasmic mutations. Since such mutations may have pathological consequences, they likely survive and are inherited due to functional complementation via the intracellular mitochondrial network. Here, we hypothesized that compromised mitochondrial fusion would hamper such complementation, thereby affecting heteroplasmy inheritance. RESULTS: We assessed heteroplasmy levels in three Caenorhabditis elegans strains carrying different heteroplasmic mtDNA deletions (ΔmtDNA) in the background of mutant mitofusin (fzo-1). Animals displayed severe embryonic lethality and developmental delay. Strikingly, observed phenotypes were relieved during subsequent generations in association with complete loss of ΔmtDNA molecules. Moreover, deletion loss rates were negatively correlated with the size of mtDNA deletions, suggesting that mitochondrial fusion is essential and sensitive to the nature of the heteroplasmic mtDNA mutations. Introducing the ΔmtDNA into a fzo-1;pdr-1;+/ΔmtDNA (PARKIN ortholog) double mutant resulted in a skewed Mendelian progeny distribution, in contrast to the normal distribution in the fzo-1;+/ΔmtDNA mutant, and severely reduced brood size. Notably, the ΔmtDNA was lost across generations in association with improved phenotypes. CONCLUSIONS: Taken together, our findings show that when mitochondrial fusion is compromised, deleterious heteroplasmic mutations cannot evade natural selection while inherited through generations. Moreover, our findings underline the importance of cross-talk between mitochondrial fusion and mitophagy in modulating the inheritance of mtDNA heteroplasmy.

Optimal stopover model: A state-dependent habitat selection model for staging passerines.

During their seasonal migration, birds stage in areas comprising stopover sites of varying quality. Given that migrating birds have a limited information about their environment, they may land at a low-quality stopover site in which their fuel deposition rate (FDR) is low. Birds landing at such sites should decide either to extend their stopover duration or to quickly depart in search for a better site. These decisions, however, strongly depend on their body condition upon landing. To understand the decision-making process of passerines within a stopover area, comprising stopover sites of varying quality, prior to the crossing of a large ecological barrier, we constructed a state-dependent habitat selection model. The model assumes that even if migrating birds have an expectation of encountered area quality, they cannot control for their initial landing site. Once landing, movement between low- and high-quality stopover sites will occur only if the body condition of these birds is high to the extent that they can entail the energetic cost of movement. Birds in the model aim to maximize their fuel load at the end of the stopover period, to suffice for successfully crossing a large ecological barrier. The model is based on empirical data on autumn migrating Blackcaps Sylvia atricapilla, collected at two important stopover sites in the Negev desert of Israel. Migrating passerines staging at these two sites differ in their FDR and body condition. The model shows that the optimal behaviour when arriving at a low-quality stopover site is to abandon it quickly. However, as lean individuals cannot entail the costs of searching for an alternative site, they have no other choice but to stay there even if their chances to successfully cross the Sahara Desert ahead are low. Our model can be applied to other ecological systems. Proper use of this model may allow good assessment of stopover site quality, as indicated by the bird's FDR, regardless of specific site characteristics. Hence, it can help applying targeted management decisions regarding the maintenance of stopover sites or establishment of new ones.

High resilience of the mycorrhizal community to prescribed seasonal burnings in eastern Mediterranean woodlands.

Fire effects on ecosystems range from destruction of aboveground vegetation to direct and indirect effects on belowground microorganisms. Although variation in such effects is expected to be related to fire severity, another potentially important and poorly understood factor is the effect of fire seasonality on soil microorganisms. We carried out a large-scale field experiment examining the effects of spring (early-dry season) versus autumn (late-dry- season) burns on the community composition of soil fungi in a typical Mediterranean woodland. Although the intensity and severity of our prescribed burns were largely consistent between the two burning seasons, we detected differential fire season effects on the composition of the soil fungal community, driven by changes in the saprotrophic fungal guild. The community composition of ectomycorrhizal fungi, assayed both in pine seedling bioassays and from soil sequencing, appeared to be resilient to the variation inflicted by seasonal fires. Since changes in the soil saprotrophic fungal community can directly influence carbon emission and decomposition rates, we suggest that regardless of their intensity and severity, seasonal fires may cause changes in ecosystem functioning.

Small-scale spatial variability in the distribution of ectomycorrhizal fungi affects plant performance and fungal diversity.

The effects of spatial heterogeneity in negative biological interactions on individual performance and species diversity have been studied extensively. However, little is known about the respective effects involving positive biological interactions, including the symbiosis between plants and ectomycorrhizal (EM) fungi. Using a greenhouse bioassay, we explored how spatial heterogeneity of natural soil inoculum influences the performance of pine seedlings and composition of their root-associated EM fungi. When the inoculum was homogenously distributed, a single EM fungal taxon dominated the roots of most pine seedlings, reducing the diversity of EM fungi at the treatment level, while substantially improving pine seedling performance. In contrast, clumped inoculum allowed the proliferation of several different EM fungi, increasing the overall EM fungal diversity. The most dominant EM fungal taxon detected in the homogeneous treatment was also a highly beneficial mutualist, implying that the trade-off between competitive ability and mutualistic capacity does not always exist.

Modelling the effects of spatial heterogeneity and temporal variation in extinction probability on mosquito populations.

Spatial synchrony plays an important role in dictating the dynamics of spatial and stage-structured populations. Here we argue that, unlike the Moran effect where spatial synchrony is driven by exogenous factors, spatial correlation in intrinsic/local-scale processes can affect the level of spatial synchrony among distinct sub-populations, and therefore the persistence of the entire population. To explore this mechanism, we modelled the consequences of spatial heterogeneity in aquatic habitat quality, and that of temporal variation in local extinction probability, on the persistence of stage-structured mosquito populations. As a model system, we used two widely distributed mosquito species, Aedes albopictus and Culex pipiens, both key vectors of a range of infectious diseases. Spatial heterogeneity in aquatic habitat quality led to increased population persistence, and this pattern was more pronounced at intermediate dispersal rates, and in the long-dispersing species (C. pipiens). The highest regional persistence was obtained at high dispersal rates. This is probably because dispersal, in our model, did not carry any additional costs. Population persistence of both species was negatively correlated with increased temporal variation in local extinction probability. These differences were stronger in the short-dispersing species (A. albopictus), especially at intermediate dispersal rates. The dispersal of A. albopictus adults in each time step was limited to the nearest habitat patches, weakening the positive effect of spatial heterogeneity in aquatic habitat quality on population persistence. In contrast, C. pipiens adults could disperse into more remote sub-populations, resulting in much higher recolonization rates. Hence, the negative effect of temporal variation in local extinction probability on patch occupancy disappeared at intermediate dispersal rates. We suggest that effectively controlling these two mosquito species requires making few spatially synchronized control efforts (i.e., generating high temporal variation in local extinction probability), rather than many asynchronized local control efforts. Finally, our model can be easily fitted to other organisms characterized by complex life cycles, and it can be also used to examine alternative scenarios, including the effect of spatial configuration of local habitat patches and dispersal kernel shape on population persistence.

Wild boars as spore dispersal agents of ectomycorrhizal fungi: consequences for community composition at different habitat types.

The success of dispersal events depend on the organism's ability to reach and establish in a new habitat. In symbiotic organisms, establishment also depends on the presence of their symbiont partner in the new habitat. For instance, the establishment of obligate ectomycorrhizal (EM) trees outside the forest is largely limited by the presence of EM fungi in soil. Wild boars (Sus scrofa) are important dispersal agents of EM fungal spores, particularly in the moderately dry Mediterranean region. The aim of this study was to explore how EM fungal spores dispersed by wild boars influence the EM fungal community associated with the roots of Pinus halepensis seedlings at different habitat types. Using a greenhouse bioassay, we grew pine seedlings in two soil types: old-field and forest soils mixed with either natural or autoclaved wild boar feces. In both soils, we observed a community dominated by a few EM fungal species. Geopora (85 %) and Suillus (68 %) species dominated the forest and old-field soils, respectively. The addition of natural wild boar feces increased the abundance of Tuber species in both EM fungal communities. However, this effect was more pronounced in pots with old-field soil, leading to a more even community, equally dominated by both Tuber and Suillus species. In forest soil, Geopora maintained dominance, but decreased in abundance (67 %), due to the addition of Tuber species. Our findings indicate that wild boar feces can be an important source for EM inoculum, especially in habitats poor in EM fungi such as old-fields.

Foraging syndromes and trait variation in antlions along a climatic gradient.

Behavioral syndromes arise when individual behavior is correlated over time and/or across environmental contexts, often resulting in inter-population behavioral differences. Three main hypotheses have been suggested to explain the evolution of behavioral syndromes. The constraint hypothesis suggests that behaviors originate from a shared mechanism with a strong genetic or physiological basis. In contrast, according to the adaptive hypothesis, behavioral syndromes depend on specific selective pressures in each environment, and thus should evolve when specific behavioral combinations are advantageous. Finally, behavioral syndromes can also arise owing to neutral stochastic processes. We tested here for variation in the foraging syndromes of pit-building antlions originating from different populations along a climatic gradient. Although inter-population variation existed in some traits, foraging syndromes were similar across populations, supporting the constraint hypothesis. These findings suggest that stabilizing selection, acting on the foraging behavior of antlions during their larval phase, outweighs local selection pressures, resulting in "constraint syndromes." We also explored behavioral repeatability of foraging-related traits within and among habitats (natural, novel and disturbed habitats), and detected different levels of repeatability: pit diameter was more repeatable than response time to prey, followed by prey exploitation efficiency. Behavioral repeatability of the same trait differed according to context, suggesting that repeatability is a trait in itself and should not be considered identical even when studying the same behavioral trait.

Temporal force governs the microbial assembly associated with Ulva fasciata (Chlorophyta) from an integrated multi-trophic aquaculture system.

Ulva spp., one of the most important providers of marine ecosystem services, has gained substantial attention lately in both ecological and applicational aspects. It is known that macroalgae and their associated microbial community form an inseparable unit whose intimate relationship can affect the wellbeing of both. Different cultivation systems, such as integrated multi-trophic aquaculture (IMTA), are assumed to impact Ulva bacterial community significantly in terms of compositional guilds. However, in such a highly dynamic environment, it is crucial to determine how the community dynamics change over time. In the current study, we characterized the microbiota associated with Ulva fasciata grown as a biofilter in an IMTA system in the Gulf of Aqaba (Eilat, Israel) over a developmental period of 5 weeks. The Ulva-associated microbial community was identified using the 16S rRNA gene amplicon sequencing technique, and ecological indices were further analyzed. The Ulva-associated microbiome revealed a swift change in composition along the temporal succession, with clusters of distinct communities for each timepoint. Proteobacteria, Bacteroidetes, Planctomycetes, and Deinococcus-Thermus, the most abundant phyla that accounted for up to 95% of all the amplicon sequence variants (ASVs) found, appeared in all weeks. Further analyses highlighted microbial biomarkers representing each timepoint and their characteristics. Finally, the presence of highly abundant species in Ulva microbiota yet underestimated in previous research (such as phyla Deinococcus-Thermus, families Saprospiraceae, Thiohalorhabdaceae, and Pirellulaceae) suggests that more attention should be paid to the temporal succession of the assembly of microbes inhabiting macroalgae in aquaculture, in general, and IMTA, in particular. Characterizing bacterial communities associated with Ulva fasciata from an IMTA system provided a better understanding of their associated microbial dynamics and revealed this macroalgae's adaptation to such a habitat.

Ecological insights into the resilience of marine plastisphere throughout a storm disturbance.

Among numerous research about marine plastisphere, the community living on the surface of plastic debris, little attention was given to the ecological mechanisms governing prokaryotes compared to eukaryotes, and even less focused on their resilience in a changing climate with more storm prevalence. Our current research recruited an integrated approach involving community succession across temporal dimension, ecological mechanisms that govern the assembly, and resilience to environmental perturbations to highlight the ecology of different kingdoms in the plastisphere. Towards this goal, we examined the succession of the prokaryotic and eukaryotic communities on artificial plastic nets in a sidestream of seawater from the Gulf of Aqaba over 35 days. A robust local storm enabled investigation of the alterations before, during, and after this disturbance, aiming at the community's potential to recover. Data from 16S and 18S rRNA sequencing and microscopic analyses decrypted the plastisphere diversity, community assembly, and stochasticity, followed by further analyses of functional and co-occurrence networks for the prokaryotic group. Prokaryotic and eukaryotic communities underwent exact opposite ecological mechanisms. While determinism driven by a robust environmental selection dictated the prokaryotic community assembly, stochasticity prevailed when this condition was relaxed. Interestingly, resilience against disturbance was observed in prokaryotes but not in eukaryotes. The decrease in compositional, functional diversity and network complexity in the prokaryotic community was reversed, presumably due to the niche specification process and high dispersal. Niche specification following perturbation was evident in some bacteria by selected functions associated with plastic degradation, stress response, and antibiotic resistance. On the contrary, eukaryotes decreased in diversity and were dominated by the commonly found Chlorophyta towards the later successional period. Novel findings on the ecology of marine plastisphere during perturbation encourage the integration of this aspect into prediction research.

An experimental critique of the population carrying capacity concept.

Carrying capacity has multiple definitions, but nowadays, it is mainly referred to as the maximum number of individuals of a particular species sustained by the environment. We examined whether multiple populations of two flour beetle species grown under controlled laboratory conditions reach similar asymptotic population sizes when provided with similar amounts of food resources. We demonstrate that the variation in the asymptotic population sizes was considerably larger than that of the initial food resources and that the latter had no significant effect on the former. Our results experimentally contribute to past literature criticizing the carrying capacity concept, demonstrating that there is no single carrying capacity even under strict laboratory conditions. Therefore, we should not expect to often find "carrying capacities" in nature, where resources fluctuate over time, and interspecific interactions are ubiquitous. We suggest that the classic meaning of carrying capacity should be revisited or saved chiefly for didactic purposes.

Evidence for competition and cannibalism in wormlions.

Trap-building predators, such as web-building spiders and pit-building antlions, construct traps to capture their prey. These predators compete over sites that either enable the construction of suitable traps, are prey rich, or simply satisfy their abiotic requirements. We examined the effect of intraspecific competition over suitable space in pit-building wormlions. As expected, the ability of wormlions to select their favorable microhabitats-shaded or deep sand over lit or shallow sand-decreased with increasing density. Favorable microhabitats were populated more frequently by large than by small individuals and the density of individuals in the favorable microhabitat decreased with their increase in body mass. The advantage of large individuals in populating favorable microhabitats is nevertheless not absolute: both size categories constructed smaller pits when competing over a limited space compared to those constructed in isolation. The outcome of competition also depends on the type of habitat: deep sand is more important for large wormlions than small ones, while shade is similarly important for both size classes. Finally, in contrast to previous reports, cannibalism is shown here to be possible in wormlions. Its prevalence however is much lower compared to that documented in other trap-building predators. Our findings show that the advantage of large individuals over small ones should not be taken for granted, as it can depend on the environmental context. We present suggestions for the relative lack of competitive advantage of large wormlion individuals compared to other trap-building predators, which may stem from the absence of obvious weaponry, such as sharp mandibles.

Copy number variation of the SELENBP1 gene in schizophrenia.

BACKGROUND: Schizophrenia is associated with rare copy-number (CN) mutations. Screening for such alleles genome-wide, though comprehensive, cannot study in-depth the causality of particular loci, therefore cannot provide the functional interpretation for the disease etiology. We hypothesized that CN mutations in the SELENBP1 locus could associate with the disorder and that these mutations could alter the gene product's activity in patients. METHODS: We analyzed SELENBP1 CN variation (CNV) in blood DNA from 49 schizophrenia patients and 49 controls (cohort A). Since CN of genes may vary among tissues, we investigated SELENBP1 CN in age- sex- and postmortem interval-matched cerebellar DNA samples from 14 patients and 14 controls (cohort B). Since CNV may either be de-novo or inherited we analyzed CNV of the SELENBP1 locus in blood DNA from 26 trios of schizophrenia probands and their healthy parents (cohort C). SELENBP1 mRNA levels were measured by real-time PCR. RESULTS: In cohort A reduced CN of the SELENBP1 locus was found in four patients but in none of the controls. In cohort B we found reduced CN of the SELENBP1 locus in two patients but in none of the controls. In cohort C three patients exhibited drastic CN reduction, not present in their parents, indicating de-novo mutation. A reduction in SELENBP1 mRNA levels in the postmortem cerebellar samples of schizophrenia patients was found. CONCLUSIONS: We report a focused study of CN mutations in the selenium binding-protein1 (SELENBP1) locus previously linked with schizophrenia. We provide evidence for recurrence of decreased CN of the SELENBP1 locus in three unrelated patients' cohorts but not in controls, raising the possibility of functional involvement of these mutations in the etiology of the disease.

Conservation genetics of a rare Gerbil species: a comparison of the population genetic structures and demographic histories of the locally rare Pygmy Gerbil and the common Anderson's Gerbil.

BACKGROUND: One of the major challenges in evolutionary biology is identifying rare species and devising management plans to protect them while also sustaining their genetic diversity. However, in attempting a broad understanding of rarity, single-species studies provide limited insights because they do not reveal whether the factors that affect rare species differ from those that affect more common species. To illustrate this important concept and to arrive at a better understanding of the form of rarity characterizing the rare Gerbillus henleyi, we explored its population genetic structure alongside that of the locally common Gerbillus andersoni allenbyi. We trapped gerbils in several locations in Israel's western and inner Negev sand dunes. We then extracted DNA from ear samples, and amplified two mitochondrial sequences: the control region (CR) and the cytochrome oxidase 2 gene (CO2). RESULTS: Nucleotide diversity was low for all sequences, especially for the CR of G. a. allenbyi, which showed no diversity. We could not detect any significant population genetic structure in G. henleyi. In contrast, G. a. allenbyi's CO2 sequence showed significant population genetic structure. Pairwise PhiPT comparisons showed low values for G. henleyi but high values for G. a. allenbyi. Analysis of the species' demographic history indicated that G. henleyi's population size has not changed recently, and is under the influence of an ongoing bottleneck. The same analysis for G. a. allenbyi showed that this species has undergone a recent population expansion. CONCLUSIONS: Comparing the two species, the populations of G. a. allenbyi are more isolated from each other, likely due to the high habitat specificity characterizing this species. The bottleneck pattern found in G. henleyi may be the result of competition with larger gerbil species. This result, together with the broad habitat use and high turnover rate characterizing G. henleyi, may explain the low level of differentiation among its populations. The evidence for a recent population expansion of G. a. allenbyi fits well with known geomorphological data about the formation of the Negev sand dunes and paleontological data about this species' expansion throughout the Levant. In conclusion, we suggest that adopting a comparative approach as presented here can markedly improve our understanding of the causes and effects of rarity, which in turn can allow us to better protect biodiversity patterns.

Parental diabetes status reveals association of mitochondrial DNA haplogroup J1 with type 2 diabetes.

BACKGROUND: Although mitochondrial dysfunction is consistently manifested in patients with Type 2 Diabetes mellitus (T2DM), the association of mitochondrial DNA (mtDNA) sequence variants with T2DM varies among populations. These differences might stem from differing environmental influences among populations. However, other potentially important considerations emanate from the very nature of mitochondrial genetics, namely the notable high degree of partitioning in the distribution of human mtDNA variants among populations, as well as the interaction of mtDNA and nuclear DNA-encoded factors working in concert to govern mitochondrial function. We hypothesized that association of mtDNA genetic variants with T2DM could be revealed while controlling for the effect of additional inherited factors, reflected in family history information. METHODS: To test this hypothesis we set out to investigate whether mtDNA genetic variants will be differentially associated with T2DM depending on the diabetes status of the parents. To this end, association of mtDNA genetic backgrounds (haplogroups) with T2DM was assessed in 1055 Jewish patients with and without T2DM parents ('DP' and 'HP', respectively). RESULTS: Haplogroup J1 was found to be 2.4 fold under-represented in the 'HP' patients (p = 0.0035). These results are consistent with a previous observation made in Finnish T2DM patients. Moreover, assessing the haplogroup distribution in 'DP' versus 'HP' patients having diabetic siblings revealed that haplogroup J1 was virtually absent in the 'HP' group. CONCLUSION: These results imply the involvement of inherited factors, which modulate the susceptibility of haplogroup J1 to T2DM.

A morphological and life history comparison between desert populations of a sit-and-pursue antlion, in reference to a co-occurring pit-building antlion.

Although most antlion species do not construct pits, the vast majority of studies on antlions focused on pit-building species. We report here on a transplant experiment aiming to test for morphological and life history differences between two desert populations of a sit-and-pursue antlion species, Lopezus fedtschenkoi (Neuroptera: Myrmeleontidae), originating from habitats, which mainly differ in plant cover and productivity. We raised the antlion larvae in environmental chambers simulating either hyper-arid or Mediterranean climate. We found significant differences in the morphology and life history of L. fedtschenkoi larvae between the two populations. For example, the larvae originating from the more productive habitat pupated faster and had a higher growth rate. In agreement with the temperature-size rule, antlions reached higher final mass in the colder Mediterranean climate and exhibited a higher growth rate, but there was no difference in their developmental time. Observed differences in morphology between populations as well as those triggered by climate growing conditions could be explained by differences in size allometry. We also provide a quantitative description of the allometric growth axis, based on 12 morphological traits. Comparing the responses of L. fedtschenkoi with those observed in a co-occurring pit-building antlion indicated that there were neither shape differences that are independent of size nor was there a difference in the plasticity level between the two species.

A trade-off between growth and starvation endurance in a pit-building antlion.

Trade-offs have a central role in evolutionary ecology and life-history theory. Here, we present evidence for the existence of a rarely studied trade-off between growth rate and starvation endurance in larvae of a pit-building antlion. We first manipulated antlions' feeding regime and obtained a spectrum of growth rates. Next, we starved the antlions and documented their rate of mass loss. Antlions growing faster during the feeding phase also lost mass faster during the successive starvation period, implying the existence of an induced trade-off between fast growth and starvation endurance. Finally, we fed all antlions with prey items of similar mass and measured both the giving-up prey mass (i.e. the remaining body mass of the prey that was not converted into predator body mass), and growth efficiency of antlions (i.e. proportion of prey consumed, negatively correlated with giving-up prey mass). The giving-up mass was negatively correlated with the growth rate of the antlions during the feeding phase, and positively correlated with their growth rate during the starvation phase (the opposite pattern was evident when examining growth efficiency), incongruently with the common phenomenon of growth compensation (i.e. extracting more of the prey after a starvation period). We suggest that antlion larvae can adopt a physiological mode bounded by two extremes: one extreme is adapted to starvation, involving reduced metabolic rates but also reduced capability to exploit prey, while the other is adapted to fast growth, allowing an efficient exploitation of prey, but at the expense of lowered starvation endurance.

Phenotypic plasticity and variation in morphological and life-history traits of antlion adults across a climatic gradient.

We report here on two complementary experiments examining the effect of climate on morphological and life-history traits of antlion adults. We first examined whether body size and wing loading of emerging adults are plastic by raising larvae, collected from five antlion populations along Israel's sharp climatic gradient, in two environmental chambers simulating temperature and humidity of desert and Mediterranean climates. The variance in adult morphology was mostly related to body size, with adults of Mediterranean populations being larger than those of desert populations. Wing-to-thorax ratio was negatively correlated with temperature, compensating for the decrease in wing-beat frequency in colder environments. Differences between climatic treatments were significant for body size but not for the wing-to-thorax ratio, suggesting that body size is more plastic than the ratio between different body components. We next investigated how the exposure of antlion pupae to different climatic conditions influences the emerging adults. Adult body mass increased with final larval body mass at a faster rate when exposed to Mediterranean rather than desert conditions. Duration of the pupa stage was positively correlated with final larval mass, but only under Mediterranean conditions. Adult survival increased with initial mass (after eclosion), but was lower under desert conditions. Similarly, adults lost mass at a faster rate when exposed to desert conditions. Notably, the exposure of the pupae to varying climatic conditions had no effect on adult morphology. Climate is a major factor affecting insect life span and body size. Since body size is strongly linked to fecundity and survival, climate thus has a twofold effect on fitness: directly, and indirectly through body size.

Core gut microbial communities are maintained by beneficial interactions and strain variability in fish.

The term core microbiome describes microbes that are consistently present in a particular habitat. If the conditions in that habitat are highly variable, core microbes may also be considered to be ecological generalists. However, little is known about whether metabolic competition and microbial interactions influence the ability of some microbes to persist in the core microbiome while others cannot. We investigated microbial communities at three sites in the guts of European seabass under four dietary conditions. We identified generalist core microbial populations in each gut site that are shared across fish, present under multiple diets and persistent over time. We found that core microbes tend to show synergistic growth in co-culture, and low levels of predicted and validated metabolic competition. Within core microbial species, we found high levels of intraspecific variability and strain-specific habitat specialization. Thus, both intraspecific variability and interspecific facilitation may contribute to the ecological stability of the animal core microbiome.