RESUMO
Two new, i.e., 1 and 2, and 69 known phenolics were isolated from the aerial parts of Abies nephrolepis. These chemical constituents included 22 lignans, 30 flavonoids, and 19 other phenols. Their structures were determined mainly by analysis of the 1D- and 2D-NMR spectroscopic data. All the 71 isolates were evaluated for their inhibitory activities against lipopolysaccharide (LPS)-induced NO production in RAW 264.7 macrophages. Compound 1 exhibited a potent effect with an IC(50) value of 13.7 µg/ml.
Assuntos
Abies/química , Anti-Inflamatórios não Esteroides/isolamento & purificação , Óxido Nítrico/antagonistas & inibidores , Fenóis/isolamento & purificação , Extratos Vegetais/isolamento & purificação , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Anti-Inflamatórios não Esteroides/toxicidade , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Camundongos , Estrutura Molecular , Fenóis/farmacologia , Fenóis/toxicidade , Componentes Aéreos da Planta/química , Extratos Vegetais/farmacologiaRESUMO
Fourteen sesquiterpenoids including six new analogues (1-6) were isolated from the aerial parts of Inula lineariifolia.. The structures of the new sesquiterpenoids were determined by spectroscopic data analysis, and the structures of 1 and 6 were confirmed by single-crystal X-ray diffraction data and the modified Mosher method. All 14 compounds were examined for the inhibition of LPS-induced nitric oxide production in RAW264.7 macrophages. Compounds 6, 13, and 14 were found to inhibit nitric oxide production potently, with IC(50) values of 0.11, 0.25, and 0.10 microM, respectively.