RESUMO
PURPOSE: To explore the impact of UDP-glucuronosyltransferase polymorphisms (UGT1A9-118(dT) 9/10 , UGT1A9 CI399T, UGT1A9 C-440T and UGT2B7 G211T) on the pharmacokinetics of mycophenolic acid (MPA) in healthy Chinese volunteers. METHODS: We recruited ten healthy volunteers with no polymorphisms (control group), 11 homozygotes of mutants UGT1A9 CI399T and UGT1A9-118(dT) 9/10 , ten heterozygotes of UGT1A9 C440T and seven carriers of UGT2B7 211T from a total of 518 healthy Chinese volunteers. All the volunteers were orally administered a single dose of 1.5 g mycophenolate mofetil (MMF) after an overnight fast. Plasma was then collected 72 h after MMF administration. MPA, MPA-7-O-glucuronide (MPAG) and its acylglucuronide (AcMPAG) were detected by ultra-pressure liquid chromatography with UV detection. RESULTS: Compared with the control group, the UGT1A9 CI399T and UGT1A9-118(dT) 9/10 mutant homozygotes had higher MPAG plasma concentrations. Subjects with UGT1A9-440TC had enhanced MPA exposure while carriers of UGT2B7 211T had higher concentrations of the toxic metabolite, AcMPAG. CONCLUSIONS: The current results indicate that UGT1A9 and UGT2B7 genotypes could significantly alter MPA pharmacokinetics in healthy Chinese volunteers after a single oral dose of MMF.
Assuntos
Glucuronosiltransferase/genética , Imunossupressores/farmacocinética , Ácido Micofenólico/análogos & derivados , Polimorfismo Genético , Administração Oral , Povo Asiático , Frequência do Gene , Genótipo , Humanos , Imunossupressores/administração & dosagem , Imunossupressores/sangue , Desequilíbrio de Ligação , Masculino , Ácido Micofenólico/administração & dosagem , Ácido Micofenólico/sangue , Ácido Micofenólico/farmacocinética , UDP-Glucuronosiltransferase 1A , Adulto JovemRESUMO
Uridinediphosphoglucuronate-glucuronosyltransferase (UGT) is the most important enzyme of the body in phase II metabolism. UGT is widely distributed in multiple tissues, including liver, kidney and intestine, which metabolizes a large number of exogenous toxic substances and endogenous substances. Studies found that UGT1 A genetic polymorphism is one of the important reasons for the variability of glucuronic acid metabolism between individuals. This paper reviews the current concept and new advances on UGT gene mutation.