RESUMO
The pseudo-trisaccharide allosamidin 1 is a potent inhibitor of all family-18 chitinases, and it is confirmed to have insecticidal and antifungal activities. But the synthesis of allosamidins is very difficult, and it is a challengeable subject. Allosamidins were synthesized in solid-liquid phase, total solid-phase and total liquid-phase, respectively. Solid-liquid phase method realizes the partial solid-phase synthesis of allosamidins. Total solid-phase method greatly simplifies the purification process. Total liquid-phase method shortens the synthetic steps of allosamidins. The insecticidal and antifungal activities of allosamidins were also reported herein.
Assuntos
Acetilglucosamina/análogos & derivados , Antifúngicos/farmacologia , Inibidores Enzimáticos/farmacologia , Inseticidas/farmacologia , Trissacarídeos/síntese química , Trissacarídeos/farmacologia , Acetilglucosamina/síntese química , Acetilglucosamina/química , Acetilglucosamina/farmacologia , Animais , Antifúngicos/síntese química , Antifúngicos/química , Bombyx/efeitos dos fármacos , Configuração de Carboidratos , Quitinases/antagonistas & inibidores , Quitinases/metabolismo , Inibidores Enzimáticos/síntese química , Inibidores Enzimáticos/química , Fungos/efeitos dos fármacos , Inseticidas/síntese química , Inseticidas/química , Spodoptera/efeitos dos fármacos , Trissacarídeos/químicaRESUMO
Preparation of polysaccharides from the wax gourd was studied. The crude polysaccharides were extracted by ethanol precipitation, and deproteinized by the hydrochloric acid method. The deproteinized polysaccharides were separated by column chromatography to obtain the pure polysaccharides. The pure polysaccharides have a ß-D-pyranosidic bond, and their molecular weight distribution is about 22,500. It was indicated that the final product had much more purity by IR spectrum analysis, UV absorption spectrum analysis, and phenol-sulfuric acid method, respectively. It was proved that wax gourd polysaccharides were composed of rhamnose, xylose, arabinose, mannose, glucose, and galactose by thin layer chromatography.
Assuntos
Cucurbitaceae/química , Polissacarídeos/química , Proteínas de Plantas/químicaRESUMO
Polysaccharide is a kind of biological material, which has good biocompatibility, non-toxicity, and non-immunogenicity. So, the polysaccharide has widely been applied in drug delivery system. The applications of chitosan, hyaluronic acid and dextran in drug delivery have been summarized herein.
Assuntos
Antineoplásicos/administração & dosagem , Quitosana/química , Dextranos/química , Portadores de Fármacos/química , Ácido Hialurônico/química , Antineoplásicos/química , Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Interações Hidrofóbicas e Hidrofílicas , Estrutura Molecular , Nanopartículas/químicaRESUMO
Glycoarrays are very useful tools to investigate the carbohydrate-protein interactions in high-throughput manner. Carbohydrates can be immobilized on the support surface by covalent or noncovalent binding. The biological and medical uses of glycoarrays were summarized herein.
Assuntos
Carboidratos/química , Ensaios de Triagem em Larga Escala/métodos , Análise em Microsséries/métodos , Animais , Tecnologia Biomédica , Desenho de Equipamento , Ensaios de Triagem em Larga Escala/instrumentação , Humanos , Análise em Microsséries/instrumentaçãoRESUMO
Glyconanoparticles are very useful tools for proteomic and glycomics research. They mainly contain glycosylated gold nanoparticles, glycosylated quantum dots, and magnetic glyconanoparticles. This review summarized the glyconanoparticle progress on biolabeling, in vitro or in vivo imaging, biosensing, targeted drug delivery, and other biomedical applications in recent years. The core of glyconanoparticle applications is to study the carbohydrate-mediated interactions, which opens the new field in glycobiology.