Self-pulsing in kilowatt level narrow-linewidth fiber amplifier with WNS phase-modulation.

The self-pulsing phenomenon in kilowatt level narrow-linewidth fiber amplifiers with white noise source (WNS) phase-modulation is observed experimentally. It possesses the obvious threshold of the pump power and prevents the narrow-linewidth fiber lasers from further power scaling. The experimental study shows that known explanations are not applicable here and indicates that occurrence of self-pulsing is closely related to Stimulated Brillouin Scattering (SBS) process. The theoretical discussion reveals that the spikes in the modulated spectrum are the critical factor that SBS threshold is lower than the theoretical estimation. The 1+1 dimensional SBS model analysis predicts that self-pulsing originates from forward second order Stokes pulses, which is in good qualitative agreement with the experimental data.

6.5 GHz linearly polarized kilowatt fiber amplifier based on active polarization control.

The polarization-dependent transverse mode properties in a few-mode nonpolarization-maintaining (non-PM) fiber amplifier with PM fiber laser seeding are investigated. In addition, the effect of spectral broadening on degeneration of the degree of polarization (DOP) is studied. The experimental results show that a high DOP can be achieved if the output laser of the non-PM fiber amplifier has good beam quality and a narrow linewidth without any broadening. A kilowatt (kW) fiber laser system with PM fiber seed and a non-PM fiber amplifier is demonstrated with the DOP at around 90% by controlling the beam quality and spectrum broadening. With the active polarization control technique, the system can achieve a kW linearly polarized output with 6.5 GHz linewidth. The M2 is around 1.1, and the polarization extinction ratio is about 14.5 dB. Unfortunately, the random self-pulsing issue arose when the output power exceeded 1093 W. We believe that a higher power of linearly polarized laser output based on adaptive polarization control in a non-PM fiber amplifier will be obtained if the self-pulsing issue is solved.

110 W 1678 nm laser based on high-efficiency optical parametric interactions pumped by high-power slab laser.

This Letter presents a high-efficiency optical parametric amplifier pumped by a high-power slab laser with approximate uniform rectangular distribution. By optimizing the overlapping, spectrum matching, and pulse synchronization for the pump and signal lasers, output power of 110.8 W at 1678 nm with corresponding conversion efficiency of 32.3% was achieved in addition to sufficient usage of the effective area in MgO doped periodically poled lithium niobate crystal. It could also provide a designable and tunable wavelength of the amplified laser in a wide infrared region.

High-power and widely tunable mid-infrared optical parametric amplification based on PPMgLN.

We report on a high-power and widely tunable optical parametric amplifier (OPA) based on PPMgLN and pumped by a pulsed 1.064 µm MOPA laser. The operating wavelength of the OPA system is continuously tunable from 2.68 to 3.07 µm by adjusting the temperature of PPMgLN crystals, with average output power varying from 74.6 to 66.7 W for 310 W of pump power, corresponding to an optical-to-optical conversion efficiency of ∼22.8% at 2.68 µm and ∼20.5% at 3.07 µm, respectively. Output beam quality factor (M2) of the OPA was measured to be <4 over the whole tuning range.

High-power, narrow-bandwidth mid-infrared PPMgLN optical parametric oscillator with a volume Bragg grating.

We report on a high-power, narrow spectral bandwidth 2.907 µm PPMgLN optical parametric oscillator (OPO) pumped by a 1.064 µm pulsed Nd:YAG MOPA laser source. Free-running operation of the OPO exhibits maximum average output power of 71.6 W at 2.907 µm with a slope efficiency of 26.7%. Broad 2.907 µm spectral bandwidth of the free-running OPO was suppressed from ~9 nm to less than 0.7 nm by using a VBG as one cavity mirror. The maximum average power was 51.7 W at 2907.55 nm for the spectrum-narrowed OPO, corresponding to a slope efficiency of 22.5%. Continuously tunable ranges of ~8 nm around 2.907 µm had been achieved via adjusting the temperatures of the VBG and PPMgLN accordingly.

Transcriptome and metabolome analyses of anthocyanin biosynthesis in post-harvest fruits of a full red-type kiwifruit (Actinidia arguta) 'Jinhongguan'.

Anthocyanin is the main component of pigment in red-fleshed kiwifruit. 'Jinhongguan' is a new cultivar of Actinidia arguta with red peel and flesh after harvest. However, the specific types of anthocyanin in the 'Jinhongguan' fruit and its biosynthesis pathways remain largely unknown. Here, the total anthocyanin content in the fruit color conversion process was determined. The results showed that total anthocyanin content increased with the deepening color of the peel and flesh. To identify the genes related to anthocyanin biosynthesis and the types of anthocyanins in the 'Jinhongguan' fruit, a combined analysis of transcriptome and anthocyanin-targeted metabolome was carried out. A total of 5751 common differentially expressed genes (DEGs) at different stages of peel and flesh were identified, of which 2767 were common up-DEGs and 2976 were common down-DEGs. KEGG and GO enrichment analyses showed that the common up-DEGs were significantly enriched in anthocyanin synthesis-related pathways, suggesting some up-DEGs are involved in anthocyanin biosynthesis. In total, 29 metabolites were detected in the flesh by anthocyanin-targeted metabolome. Among these, nine were differential accumulation metabolites (DAMs) in comparison to red flesh vs green flesh. Six DAMs were up-regulated, with five of them were cyanidins. The content of cyanidin-3-O-galactoside was much higher than that of other DAMs, making it the main pigment in 'Jinhongguan'. Moreover, a total of 36 anthocyanin synthesis-related structural genes, 27 MYB transcription factors (TFs), 37 bHLH TFs and 9 WDR TFs were screened from the common DEGs. Correlation analysis of transcriptome and metabolome revealed that 9 structural genes, 6 MYB TFs, 6 bHLH TFs and 1 WDR TF were significantly associated with cyanidin-3-O-galactoside. Further, qRT-PCR analysis demonstrated that structural genes (AaPAL3, Aa4CL3, AaCHS2/3/8/9/11, AaDFR1/2, AaANR1, UFGT3a and UFGT6b) and TFs (MYB108, bHLH30, bHLH94-1 and WD43) play important roles in cyanidin biosynthesis. Overall, this study identified cyanidin-3-O-galactoside as the main anthocyanin type and revealed key candidate genes of red coloration of post-harvest fruit in Actinidia arguta. These findings provided new insights into the color formation mechanism of post-harvest fruit and offered a theoretical basis for color regulation in kiwifruit.

A Novel Distal Micromotor-Based Side-Looking Intravascular Ultrasound Transducer.

Cardiovascular disease has become one of the leading causes of death in China, accounting for 45.5% of all deaths in rural areas and 43.16% in urban areas. Hence, its early diagnosis is important. With the development of intravascular imaging technology, the intravascular ultrasound (IVUS) is widely used. The available commercial mechanical rotary side-looking IVUS (SL-IVUS) transducers are driven by external motors that use long flexible shafts to transmit the rotation. However, when the transducer passes through a long-curved blood vessel, it easily causes the nonuniform rotation distortion (NURD) of the image. A catheter which contains a distal motor and sodium chloride (NaCl) solution is presented in this study as an attempt to solve such issues. The NaCl solution is used to connect the transducer and micromotor so that the motor can directly drive the transducer to rotate and acquire the information of the blood vessel. The results showed that the center frequency and -6-dB fraction bandwidth of the single element were 47 MHz and 98%, respectively. The SL-IVUS catheter consists of a distal motor, with speed stability and high resolution, and has the potential to diagnose cardiovascular disease. This novel structure can decrease the dimension at the top of the catheter and reduce the risks of clinical diagnosis.

Development of Cardiac Phased Array With Large-Size PZN-5.5 %PT Single Crystals.

In recent years, the manufacturing process of lead zinc niobate-lead titanate [Pb(Zn1/3Nb2/3)O3-PbTiO3, also called PZN-PT], has been enhanced with improvements in size, consistency, and a suitable compromise between piezoelectric properties and phase transition temperature, which means that it is possible to obtain PZN-PT single crystals in sufficient size for performance characterization studies and batch manufacturing to produce high-performance medical ultrasonic transducers. This article mainly focuses on the development of the 64-element phased array ultrasonic transducer based on novel large-size PZN-PT piezoelectric single crystals. The composition of the single crystal was chosen as PZN-5.5 %PT. The designed center frequency of the phased array is 3.0 MHz, which is suitable for cardiac ultrasound imaging. The array elements were spaced at a 0.254-mm pitch, and interconnected through a custom-designed flexible circuit. Double matching layers with a light backing structure were applied in the transducer fabrication process to improve the performance of the array. The test results of the developed phased array showed a center frequency of 3.0 MHz, and an average -6 dB fractional bandwidth of 72%. In the vicinity of the center frequency, the two-way insertion loss (IL) was about -46 dB, while a crosstalk between the adjacent elements was less than -31 dB. The wire phantom can be distinctly imaged with the phased array, and the axial and lateral resolutions were measured to be 660 and [Formula: see text], respectively. The image of a standard phantom was acquired to present the imaging performance of the transducer. The final results indicate that the transducer arrays based on novel large-size PZN-PT single crystals are quite promising for use in medical ultrasound imaging applications.

Genome-wide analysis of MADS-box gene family in kiwifruit (Actinidia chinensis var. chinensis) and their potential role in floral sex differentiation.

Kiwifruit (Actinidia chinensis Planch.) is a functionally dioecious plant, which displays diverse morphology in male and female flowers. MADS-box is an ancient and huge gene family that plays a key role in plant floral organ differentiation. In this study, we have identified 89 MADS-box genes from A. chinensis Red 5 genome. These genes are distributed on 26 chromosomes and are classified into type I (21 genes) and type II (68 genes). Overall, type II AcMADS-box genes have more complex structures than type I with more exons, protein domains, and motifs, indicating that type II genes may have more diverse functions. Gene duplication analysis showed that most collinearity occurred in type II AcMADS-box genes, which was consistent with a large number of type II genes. Analysis of cis-acting elements in promoters showed that AcMADS-box genes are mainly associated with light and phytohormone responsiveness. The expression profile of AcMADS-box genes in different tissues showed that most genes were highly expressed in flowers. Further, the qRT-PCR analysis of the floral organ ABCDE model-related genes in male and female flowers revealed that AcMADS4, AcMADS56, and AcMADS70 were significantly expressed in female flowers. It indicated that those genes may play an important role in the sex differentiation of kiwifruit. This work provided a comprehensive analysis of the AcMADS-box genes and may help facilitate our understanding of the sex differentiation regulatory mechanism in kiwifruit.

A Novel Synchronous Micro Motor for Intravascular Ultrasound Imaging.

OBJECTIVE: Intravascular ultrasound (IVUS) is an important method for evaluating lumen dimensions and guiding intervention. However, the current IVUS catheter using a proximal motor and flexible drive shaft is easily rotated at an unstable speed when it passes through along bending vessel. One approach to solve this problem is to develop a catheter driven by a distal motor. METHODS: This paper presents a rotation device incorporating a high-frequency transducer as an attempt to facilitate this approach. A novel micro distal synchronous micro motor with 3.7 mm length and 1.2 mm outer diameter was proposed as an actuator for the IVUS catheter. A 0.5 mm × 0.5 mm Pb(Mg1/3Nb2/3)O3-PbTiO3 single crystal 1-3 composite single-element transducer was designed and manufactured. The probe is fixed to the front end of the catheter. The 45° reflector, which is opposite to the probe, was used to steer ultrasound to the tissue. RESULTS: The results showed that the maximum torque and rotation speed of the motor were 2.79 µNm and 275 revolutions per second, respectively, at a driving current of 0.34 A. The maximum angular error was 7° at 0.13 A and 30 Hz. The center frequency and -6 dB fractional bandwidth of single element were 34 MHz and 72%, respectively. At the center frequency, the two-way insertion loss was 14 dB. CONCLUSION: The integrated distal motor IVUS catheter, with small dimensions, a good torque, speed stability, and good ultrasound imaging performance, has tremendous potential in blood vessel imaging. SIGNIFICANCE: The novel structure of the catheter could facilitate endoluminal sonography, reducing risks of the clinical diagnosis.

Miniaturized High-Resolution Integrated 360° Electronic Radial Ultrasound Endoscope for Digestive Tract Imaging.

To improve the accuracy and operating flexibility of digestive diagnostic applications in situ, this paper demonstrates the design, fabrication, and evaluation of a miniaturized high-resolution integrated 360° electronic radial ultrasound endoscope. To improve the uniformity between elements of the radial array transducer, an optimal fabrication procedure based on a flexible matching layer was developed. The radial ultrasound endoscope has a 128.3° 5-million-pixel camera module integrated with an approximately 8-MHz 128-element radial ultrasonic array in a package with an outer diameter of 9.5 mm. The results showed that the array has a center frequency of 8.2 MHz, a -6-dB fractional bandwidth of 83.4%, and a two-way insertion loss of 39.62 dB at the center frequency. Annulus and wire phantoms can be distinctly imaged with the radial array. Printed eight-point font can be distinctly imaged with both a large view and a 3-18-mm depth of field in lumen short-range optical imaging. A porcine esophagus can be distinctly imaged in vitro with the integrated endoscope. The results indicate that the fabrication method based on a flexible matching layer can achieve high uniformity between elements of a radial array transducer. In addition, the proposed ultrasound endoscope demonstrates a good image resolution.

Fabrication and Performance of a Miniaturized and Integrated Endoscope Ultrasound Convex Array for Digestive Tract Imaging.

OBJECTIVE: this work presents the design, fabrication, and testing of a miniaturized and integrated ultrasound endoscope for use as an in situ digestive diagnostic device to facilitate real-time ultrasound guidance of intervention treatments. METHODS: we designed an optimal structure to integrate an auto-focus 5-megapixel camera module with an 8-MHz, 64-element curvilinear ultrasonic array in one miniaturized package. A novel three-axis auto-focusing voice coil motor (VCM) was designed and manufactured for the camera module to move the lens position for auto-focusing and to adjust the lens tilt. RESULTS: the results showed that the array had a center frequency of 8.09 MHz and a -6-dB fractional bandwidth of 83%. At the center frequency, the two-way insertion loss was 40.6 dB. Endoscopic ultrasound imaging demonstrated satisfactory performance for imaging an anthropomorphic phantom of the esophagus. By slightly adjusting the tilt angle of the optical axis of the lens, the optical image captured by the auto-focusing lens obtained improved definition regardless of changes in the view angle of the camera with respect to the objects being captured. CONCLUSION: the integrated convex ultrasound endoscope, possessing minimal size, improved optical imaging definition, and good ultrasound imaging performance, can become a useful tool in digestive tract imaging. SIGNIFICANCE: the miniaturized and integrated convex ultrasound endoscope can facilitate real-time ultrasound intervention guidance, reducing risks associated with the operation.