Therapeutic applicability of cannabidiol and other phytocannabinoids in epilepsy, multiple sclerosis and Parkinson's disease and in comorbidity with psychiatric disorders.

Studies have demonstrated the neuroprotective effect of cannabidiol (CBD) and other Cannabis sativa L. derivatives on diseases of the central nervous system caused by their direct or indirect interaction with endocannabinoid system-related receptors and other molecular targets, such as the 5-HT1A receptor, which is a potential pharmacological target of CBD. Interestingly, CBD binding with the 5-HT1A receptor may be suitable for the treatment of epilepsies, parkinsonian syndromes and amyotrophic lateral sclerosis, in which the 5-HT1A serotonergic receptor plays a key role. The aim of this review was to provide an overview of cannabinoid effects on neurological disorders, such as epilepsy, multiple sclerosis and Parkinson's diseases, and discuss their possible mechanism of action, highlighting interactions with molecular targets and the potential neuroprotective effects of phytocannabinoids. CBD has been shown to have significant therapeutic effects on epilepsy and Parkinson's disease, while nabiximols contribute to a reduction in spasticity and are a frequent option for the treatment of multiple sclerosis. Although there are multiple theories on the therapeutic potential of cannabinoids for neurological disorders, substantially greater progress in the search for strong scientific evidence of their pharmacological effectiveness is needed.

Exploring genetic diversity in inbred papaya lines for fruit quality in advanced stage of improvement.

Despite the relevance of the global scenario regarding the papaya (Carica papaya L.) trade, there is still a limited number of papaya cultivars with different fruit patterns. Therefore, it is essential to explore the genetic variability at all levels of the germplasm used in the development of new papaya cultivars to meet its marketing goal. Thus, this study measured and explored the potential of genetic variability based on related to fruit quality traits, of a population of papaya lines in the F5 generation through several statistical analyzes. For this, 97 inbred lines obtained using the Single Seed Descent method, resulting from a cross between the JS-12 and Sekati genotypes, both with Formosa fruit pattern, were evaluated. Results indicated there was genetic variability in the fruit quality. The traits that most contributed to the variability were related to the fruit shape. The diverse population of 97 inbred papaya lines in the F5 generation showed promise for producing commercial-sized fruits in Formosa, Intermediate, and Solo patterns. Additionally, the selection of inbred papaya lines based on fruit shape using morpho-anatomical traits does not compromise physical and chemical parameters related to fruit quality.

Additive and Non-Additive Effects on the Control of Key Agronomic Traits in Popcorn Lines under Contrasting Phosphorus Conditions.

Phosphorus is a non-renewable natural resource that will run out of reserves in the upcoming decades, making it essential to understanding the inheritance of nutrient use efficiency for selecting superior genotypes. This study investigated the additive and non-additive effects of commercially relevant traits for the popcorn crop (grain yield-GY, popping expansion-PE, and expanded popcorn volume per hectare-PV) in different conditions of phosphorus (P) availability in two locations in Rio de Janeiro State, Brazil. Six S7 lines previously selected for P use-L59, L70, and P7, efficient and responsive; and L54, L75, and L80, inefficient and non-responsive-were used as testers in crosses with 15 progenies from the fifth cycle of intrapopulation recurrent selection of UENF-14, with adaptation to the North and Northwest regions of Rio de Janeiro State. Using the Griffing diallel analysis, P use efficiency was predominantly additive in the expression of PE, and non-additive effects were prominent for GY and PV. For obtaining genotypes that are efficient for phosphorus use, it is recommended that heterosis with parents that provide additive gene accumulation for PE be explored.

Discovery of SNPs and InDels in papaya genotypes and its potential for marker assisted selection of fruit quality traits.

Papaya is a tropical and climacteric fruit that is recognized for its nutritional benefits and medicinal applications. Its fruits ripen quickly and show a drastic fruit softening, leading to great post-harvest losses. To overcome this scenario, breeding programs of papaya must invest in exploring the available genetic variation to continue developing superior cultivars with improved fruit quality traits. The objective of this study was to perform a whole-genome genotyping (WGG) of papaya, predict the effects of the identified variants, and develop a list of ripening-related genes (RRGs) with linked variants. The Formosa elite lines of papaya Sekati and JS-12 were submitted to WGG with an Illumina Miseq platform. The effects of variants were predicted using the snpEff program. A total of 28,451 SNPs having Ts/Tv (Transition/Transversion) ratio of 2.45 and 1,982 small insertions/deletions (InDels) were identified. Most variant effects were predicted in non-coding regions, with only 2,104 and 138 effects placed in exons and splice site regions, respectively. A total of 106 RRGs were found to be associated with 460 variants, which may be converted into PCR markers to facilitate genetic mapping and diversity studies and to apply marker-assisted selection (MAS) for specific traits in papaya breeding programs.

Effects of bone marrow mononuclear cells on induction of axonal sprouting in cortico-cortical and cortico-striatal pathways in an animal model of cortical ablation.

OBJECTIVES: Many therapies have been proposed in order to investigate the mechanisms of neural repair associated with neurological diseases, including bone marrow mononuclear cells (BMMC) transplantation. However, there is evidence that some encephalic injuries are less responsive to neural repair, such as, for example, cortical ablation. On the other hand, some models of cortical ablation have shown functional recovery after BMMC transplantation. Thus, it is relevant to expand the knowledge of BMMC transplantation-induced neuroplasticity in animal models, considering a promising approach for the rehabilitation of patients with neurological diseases. Using an experimental model of cerebral cortex ablation in adult male Wistar rats, which is known to be poorly responsive to neuroplasticity, the aim of this study was to investigate the effects of BMMC on axonal sprouting in cortico-cortical and cortico-striatal pathways synaptic fields. An anterograde neurotracer was used to evaluate the distribution of axonal fibres. RESULTS: The results showed that BMMC were not able to significantly induce axonal sprouting in the evaluated synaptic fields. Our results reinforced the idea that cortical ablation may be less responsive to neuroplasticity and the beneficial effects of BMMC therapy depend on the particularities of a neural microenvironment intrinsic to a given cortical lesion.

The genetic diversity and population structure of two endemic Amazonian quillwort (Isoetes L.) species.

BACKGROUND: Two endemic lycophyte species Isoetes cangae and Isoetes serracarajensis have been recently described in the State of Pará in the Amazon forest located in northern Brazil. Isoetes L. has survived through three mass extinctions. Plants are considered small-sized, heterosporous, and can display a great diversity of physiological adaptations to different environments. Thus, the current study aimed to estimate the genetic variation of the populations of I. cangae and I. serracarajensis to generate information about their different mechanisms for survival at the same geographical location that could point to different reproductive, adaptative and dispersal strategies and should be considered for effective conservation strategies. METHODS: The genetic diversity and population structure of I. cangae and I. serracarajensis were investigated using Inter Simple Sequence Repeat (ISSR) molecular markers. Total genomic DNA was isolated, and the genetic diversity parameters were calculated. RESULTS: The sixteen primers produced 115 reproducible bands, 87% of which were polymorphic. A high level of polymorphic loci (81.74% and 68.48%) and a high Shannon index (Sh = 0.376 and 0.289) were observed for I. cangae and I. serracarajensis, respectively. The coefficient of genetic differentiation between population areas (GST) showed a higher value in I. serracarajensis (0.5440). Gene flow was higher in I. cangae (1.715) and lower in I. serracarajensis populations (0.419). Overall, the results further show that I. serracarajensis and I. cangae are two species with considerable genetic variation and that these differences may reflect their habitats and modes of reproduction. These results should be considered in the development of effective conservation strategies for both species.

Reciprocal recurrent selection for obtaining water-deficit tolerant maize progeny / Seleção recorrente recíproca para obtenção de progênies de milho tolerantes ao déficit hídrico

In view of the need to increase genetic variability to obtain materials with a significant capacity to drought tolerance, this study conducted a cycle of a reciprocal recurrent selection of full-sib families of maize. To this end, 64 full-sib families of maize were evaluated in two environments according to their morpho-agronomic data in a randomized block design with two replicates. It were analyzed of Male flowering (MF); Female flowering (FF); Flowering interval (IF); days for flowering (DF); Plant height (PH); Ear height (EH); number of plants at the Stand (NPS); Number of broken plant (NBrP); Number of bedded plants (NBeP); Strawing (St); Ear length (EL); Ear diameter (ED); Ear number (EN); Prolificacy (Pr); Number of diseased ears (NDE); Number of ears attacked by pests (NEP); Ear weight (EW); Yield (YIE) and Total Chlorophyll Index (TCI). The analysis of variance was performed by the F test at 5% significance level, and also the evaluation of genetic parameters. Regarding morpho-agronomic data, the analysis of variance and the analysis of genetic parameters showed that there was no interaction genotype x environment with regard to the genetic variability among the families under study. Lastly, the final selection of the superior genotypes was made on the basis of the ranking of the 40 most productive families, from which, combined with the molecular data, the 30 most productive, most drought-tolerant, and most genetically diverse ones were selected to compose the next cycle of recurrent maize selection aiming water-stress tolerance.

Tendo em vista a necessidade de aumentar a variabilidade genética para obter materiais com significativa capacidade de tolerância à seca, este estudo conduziu um ciclo de seleção recorrente recíproca de famílias de irmãos completos de milho. Para tanto, 64 famílias de irmãos completos de milho foram avaliadas em dois ambientes de acordo com seus dados morfoagronômicos em um delineamento de blocos casualizados com duas repetições. Foram analisados o florescimento masculino (MF); florescimento feminino (FF); Intervalo de florescimento (IF); dias para florescimento (DF); Altura da planta (PH); Altura da espiga (EH); número de plantas na parcela (NPS); Número de planta quebrada (NBrP); Número de plantas com acamadas (NBeP); empalhamento (St); Comprimento da espiga (EL); Diâmetro da espiga (DE); Número de espigas (EN); Prolificidade (Pr); Número de espigas doentes (EQM); Número de espigas atacadas por pragas (NEP); Peso de espiga (EW); Rendimento de grãos (YIE) e Índice de clorofila total (TCI). A análise de variância foi realizada pelo teste F com nível de significância de 5% e também pela avaliação dos parâmetros genéticos. Em relação aos dados morfoagronômicos, a análise de variância e a análise dos parâmetros genéticos mostraram que não houve interação genótipo x ambiente no que diz respeito à variabilidade genética entre as famílias em estudo. Por fim, a seleção final dos genótipos superiores foi feita com base no ranking das 40 famílias mais produtivas, das quais, combinadas com os dados moleculares, foram selecionadas as 30 mais produtivas, mais tolerantes à seca e mais geneticamente diversificadas. para compor o próximo ciclo de seleção recorrente de milho visando tolerância ao estresse hídrico.

Genetic Relationships among Tall Coconut Palm (Cocos nucifera L.) Accessions of the International Coconut Genebank for Latin America and the Caribbean (ICG-LAC), Evaluated Using Microsatellite Markers (SSRs).

The diversity and genetic relationships among two accessions of tall coconut palms collected in Brazil and seven accessions introduced from different geographic regions of the world were analyzed using 25 microsatellite primers, 19 of which were polymorphic and detected between 4 and 10 alleles per locus, with an average of 6.57. The observed and expected heterozygosity ranged from 0.25 and 0.40 in the Rennell Islands Tall (RIT) accession to 0.54 and 0.62 in the Polynesian Tall (PYT) accession. The analysis of genetic structure resulted in the formation of five distinct groups. The first group was formed by the accessions Brazilian Tall-Praia do Forte (BRTPF), Brazilian Tall-Merepe (BRTMe) and West African Tall (WAT); the second group consisted of Malaysian Tall (MLT); the third group of RIT; the fourth group of Vanuatu Tall (VTT); and the fifth group of Rotuman Tall (RTMT), Tonga Tall (TONT) and PYT. The dendrogram based on the nearest-neighbor method detected the formation of two main groups and five subgroups, indicating that the genetic relationships of the accessions are based on their geographic regions of origin. The analyses revealed genetic relationships between the accessions collected in Brazil and the accession from Africa, and among palms from South East Asia and the South Pacific, confirming the common origin of these accessions. The information obtained in this study can guide decisions on germplasm conservation activities and the efficient selection of genetically divergent parents for use in coconut breeding programs in Brazil, which are attempting to select for disease resistance, mainly to lethal yellowing, among other characteristics.

Combining ability of recombined F4 papaya lines: a strategy to select hybrid combination

The growing demand for quality of papaya by the domestic and international markets requires the development of genotypes that are capable of incorporating traits, such as high yield and fruit quality. This study estimated the genetic parameters and the specific combining ability of F4 papaya lines crossed to the SS-72/12 tester to identify genotypes with higher genetic value for hybrid production. We evaluated 169 treatments consisting of 62 topcross hybrid combinations, 97 F5 lines, and 10 controls in a 13 × 13 lattice design with five replicates and two plants per plot. The following traits were evaluated: plant height (PH), stem diameter (SD), first-fruit insertion height (FFIH), number of marketable fruits (NMF), number of deformed fruits (NDF), number of fruitless nodes (NFN), average fruit weight (FW), and yield (YLD). Differences p < 0.05 were found in most traits evaluated. The specific combining ability estimates indicate that hybrids formed by the cross between SS-72/12 tester and the following lines are promising, considering the multiple traits: UCLA08-088, UCLA08-101, UCLA08-071, UCLA08-014, UCLA08-025, UCLA08-028, UCLA08-122, UCLA08-055, UCLA08-026, and UCLA08-092. These hybrids meet the demands for domestic and international markets and may be available to producers as new papaya cultivars.

Combining ability of recombined F4 papaya lines: a strategy to select hybrid combination

The growing demand for quality of papaya by the domestic and international markets requires the development of genotypes that are capable of incorporating traits, such as high yield and fruit quality. This study estimated the genetic parameters and the specific combining ability of F4 papaya lines crossed to the SS-72/12 tester to identify genotypes with higher genetic value for hybrid production. We evaluated 169 treatments consisting of 62 topcross hybrid combinations, 97 F5 lines, and 10 controls in a 13 × 13 lattice design with five replicates and two plants per plot. The following traits were evaluated: plant height (PH), stem diameter (SD), first-fruit insertion height (FFIH), number of marketable fruits (NMF), number of deformed fruits (NDF), number of fruitless nodes (NFN), average fruit weight (FW), and yield (YLD). Differences p < 0.05 were found in most traits evaluated. The specific combining ability estimates indicate that hybrids formed by the cross between SS-72/12 tester and the following lines are promising, considering the multiple traits: UCLA08-088, UCLA08-101, UCLA08-071, UCLA08-014, UCLA08-025, UCLA08-028, UCLA08-122, UCLA08-055, UCLA08-026, and UCLA08-092. These hybrids meet the demands for domestic and international markets and may be available to producers as new papaya cultivars.(AU)

A two-location trial for selecting corn silage hybrids for the humid tropic: forage and grain yields and in vitro fermentation characteristics

The goal of our study was to evaluate the nutritional potential of dented corn hybrids for silage production. We performed a two-location trial in which 19 dented corn hybrids and five corn controls grew in four randomized blocks within two experimental areas located in the Northern (Campos dos Goytacazes) and Northwestern (Itaocara) Rio de Janeiro State, Brazil. We recorded yields of fresh and dry forage matter and yields of fresh and dry grain matter, as well as chemical composition variables. We interpreted variables by assuming a Normal distribution for yield variables and a Beta distribution for chemical composition and ratios. The SAS GLIMMIX procedure fitted the linear model under those assumptions. Dual-pool models fitted the gas production profiles generated by in vitro anaerobic fermentations. We used the nlme of R software to fit the dual-pool models and the information-theoretic approach to evaluate their quality of fit. We did a cluster analysis (NbClust of R) to group corn hybrids based on fresh and DM yields and kinetic parameters of in vitro gas production. Three clusters of corn hybrids stood out, their basic differences relied on fresh and DM yields. Nonetheless, the least-squares means for gas production characteristics among groups did not present disjoint confidence intervals. Therefore, we can infer that dented corn hybrids rank by forage yield, but not by forage quality, and recommend the most productive ones that consistently outstand in both locations (hybrids UENF-2203, UENF-2192, UENF-2193, and UENF-506-11).

Development of a gene-centered ssr atlas as a resource for papaya (Carica papaya) marker-assisted selection and population genetic studies.

Carica papaya (papaya) is an economically important tropical fruit. Molecular marker-assisted selection is an inexpensive and reliable tool that has been widely used to improve fruit quality traits and resistance against diseases. In the present study we report the development and validation of an atlas of papaya simple sequence repeat (SSR) markers. We integrated gene predictions and functional annotations to provide a gene-centered perspective for marker-assisted selection studies. Our atlas comprises 160,318 SSRs, from which 21,231 were located in genic regions (i.e. inside exons, exon-intron junctions or introns). A total of 116,453 (72.6%) of all identified repeats were successfully mapped to one of the nine papaya linkage groups. Primer pairs were designed for markers from 9,594 genes (34.5% of the papaya gene complement). Using papaya-tomato orthology assessments, we assembled a list of 300 genes (comprising 785 SSRs) potentially involved in fruit ripening. We validated our atlas by screening 73 SSR markers (including 25 fruit ripening genes), achieving 100% amplification rate and uncovering 26% polymorphism rate between the parental genotypes (Sekati and JS12). The SSR atlas presented here is the first comprehensive gene-centered collection of annotated and genome positioned papaya SSRs. These features combined with thousands of high-quality primer pairs make the atlas an important resource for the papaya research community.

Phylogeographic distribution of asperisporium caricae isolates in brazilian southeastern and northeastern coastal regions / Distribuição filogeográfica de isolados de Asperisporium caricae no litoral do sudeste e nordeste brasileiro

Papaya (Carica papaya L.) is one of the main tropical fruits consumed in Brazil. The country is also one of the main papaya exporters, but one of the factors hindering its production lies on foliar diseases such as papaya black spot, which is caused by fungus Asperisporium caricae. This pathogen is widely distributed in the main producing regions of the Brazilian coastal area. Phylogeographic studies contribute to the knowledge about the genetic diversity and geographical distribution of genealogical lineages (haplotypes) and help better understanding the reproductive and evolutionary processes of closely related species or of a single species. Such information is useful in studies about phytopathogenic fungi because it enables identifying the most prevalent genealogical lineages in a given location, as well as inferring dispersal routes and providing information on the origin and frequency of exotic material introduction events. Results in this type of study can significantly help developing new disease control strategies. Literature still lacks studies on the Papaya x A. caricae pathosystem. Based on the phylogenetic and phylogeographic analysis applied to nucleotide sequences of the Internal transcribed spacer (ITS) gene, we herein address the genealogical and dispersal events recorded for this pathogen in order to better understand its evolution in, and adaptation to, Brazilian orchards. Three haplotypes were identified among the A. caricae isolates; their distribution was mostly related to the geographic distance between sample collection regions rather than to any reproductive or evolutionary processes presented by the species. The low variability among the herein studied isolates may result from the physiological specialization (survival exclusively associated with the host plant) and from the regional transport of contaminated fruits (with lesions and spores), besides the low contribution of reproductive events, which corroborate the lack of knowledge about the sexual stages of A. caricae.

O mamoeiro (Carica papaya L.), é uma das principais frutas tropicais consumidas no Brasil. O país é um dos principais exportadores de mamão e um dos gargalos da produção mundial está diretamente ligado à ocorrência de doenças foliares, podendo-se destacar a pinta-preta do mamoeiro, causada pelo fungo Asperisporium caricae. O patógeno está amplamente distribuído nas principais regiões produtoras, as quais englobam grande parte do litoral Brasileiro. Estudos filogeográficos contribuem não só para oconhecimento da diversidade genética e da distribuição geográfica das linhagens genealógicas (haplótipos), como também contribuem para o conhecimento dos processos reprodutivos e evolutivos de uma espécie ou de espécies estreitamente relacionadas. No estudo de fungos fitopatogênicos, tais informações são úteis para identificar linhagens genealógicas mais prevalentes em um determinado local, inferir as rotas de dispersão e fornecer informações sobre a origem e frequência dos eventos de introdução de material exótico. Este tipo de estudo produz resultados que podem colaborar significativamente na elaboração de novas estratégias de controle da doença. Não existe, até o presente momento, este tipo de estudo para o patossistema Mamoeiro x A. caricae. Neste trabalho, por meio da análise filogenética e filogeográfica, a partir de sequências nucleotídicas do gene Internal transcribed spacer (ITS), discutimos os eventos genealógicos e de dispersão deste patógeno com o intuito de compreender melhor sua evolução e adaptação nos pomares brasileiros. Dentre os isolados de A. caricae foram identificados 3 haplótipos, sendo sua distribuição relacionada mais ao distanciamento geográfico das regiões das coletas das amostras do que a eventuais processos reprodutivos ou evolutivos da espécie. Acredita-se que a baixa variabilidade dentre os isolados estudados seja explicada pela especialização fisiológica (sobrevivência exclusivamente associada à planta hospedeira) e ao transporte regional de frutos contaminados (com lesões e esporos), sendo baixa a contribuição de eventos reprodutivos, o que corrobora o desconhecimento de fase sexual de A. caricae.

Papaya recombinant inbred lines selection by image-based phenotyping

The selection of superior Carica papaya (L) genotypes depends on the availability of genetic variability and on the favorable and simultaneous response of the genotypes to those traits of most interest. However, manual phenotyping (MP) demands intensive labor, is time-consuming and expensive. The aim of the current study is to access the efficiency of image-based phenotyping (IBP) in estimating genetic parameters and in selecting F4 recombinant inbred lines. The genetic parameters and values were estimated in accordance with the REML/BLUB procedure and combined selection using the selection index based on standardized genetic values. The majority of traits accessed through IBP showed experimental coefficients of variation similar to those found through MP. Both methodologies showed genetic parameters of similar magnitude, indicating expressive genetic variability between lines in the traits accessed in this study. The same superior lines were indicated in both methodologies and expressive genetic gains obtained through the lines were selected for all traits. IBP performance was similar to that of MP with respect to the estimates of breeding-relevant traits such as commercial fruits and yield. Thus, IBP showed efficient phenotypic assessment, as well as selective accuracy in accessing genetic variability and genetic gains, when it was compared to MP. Since IBP is far less dependent on labor, it is expected to be incorporated into the routine of papaya breeding programs as a way of increasing the number of accessed lines and, consequently, increasing genetic gains.

Papaya recombinant inbred lines selection by image-based phenotyping

The selection of superior Carica papaya (L) genotypes depends on the availability of genetic variability and on the favorable and simultaneous response of the genotypes to those traits of most interest. However, manual phenotyping (MP) demands intensive labor, is time-consuming and expensive. The aim of the current study is to access the efficiency of image-based phenotyping (IBP) in estimating genetic parameters and in selecting F4 recombinant inbred lines. The genetic parameters and values were estimated in accordance with the REML/BLUB procedure and combined selection using the selection index based on standardized genetic values. The majority of traits accessed through IBP showed experimental coefficients of variation similar to those found through MP. Both methodologies showed genetic parameters of similar magnitude, indicating expressive genetic variability between lines in the traits accessed in this study. The same superior lines were indicated in both methodologies and expressive genetic gains obtained through the lines were selected for all traits. IBP performance was similar to that of MP with respect to the estimates of breeding-relevant traits such as commercial fruits and yield. Thus, IBP showed efficient phenotypic assessment, as well as selective accuracy in accessing genetic variability and genetic gains, when it was compared to MP. Since IBP is far less dependent on labor, it is expected to be incorporated into the routine of papaya breeding programs as a way of increasing the number of accessed lines and, consequently, increasing genetic gains.(AU)

Diallel mixed-model analysis of papaya fruit deformities / Análise dialélica via modelos mistos das deformidades em frutos de mamoeiro

Changes in the shape of fruits from hermaphrodite flowers due to floral anomalies are quite common. The aim of this study was to estimate the effects of the general and specific combining ability on the proportions of normal and deformed (carpelloid and pentandric) papaya fruits in hermaphrodite plants using mixed models. The JS12-N, Waimanalo and Golden parents showed the potential to decrease the number of deformed fruits. In contrast, the Maradol x JS12-N, Maradol x Waimanalo, Maradol x Golden, JS12-N x JS12-4, JS12-N x Sekati, JS12-N x Waimanalo, Waimanalo x JS12-N, Waimanalo x JS12-4, Waimanalo x Golden, Waimanalo x Sunrise Solo 72/12, Golden x Maradol, Golden x JS12-N, Golden x Sekati, Golden x Waimanalo and São Mateus x JS12-N hybrids have the potential to increase the number of marketable fruits while decreasing the number of carpelloid and pentandric fruits.(AU)

Alterações na forma dos frutos originários de flores hermafroditas são bastante comuns. Estas alterações são provocadas por anomalias florais. Este trabalho teve como objetivo obter via modelos mistos estimativas de capacidade geral e específica de combinação para proporção de frutos de mamoeiro normais e com deformidades: carpeloidia e pentandria, em plantas hermafroditas. Os genitores JS12-N, Waimanalo e Golden apresentam potencial para redução de frutos com deformidades. Os híbridos Maradol x JS12-N, Maradol x Waimanalo, Maradol x Golden, JS 12-N x JS12-4, JS 12-N x Sekati, JS 12-N x Waimanalo, Waimanalo x JS12-N, Waimanalo x JS12-4, Waimanalo x Golden, Waimanalo x Sunrise Solo 72/12, Golden x Maradol, Golden x JS12-N, Golden x Sekati, Golden x Waimanalo e São Mateus x JS12-N apresentaram potencial para aumento do número de frutos comerciais e redução de frutos carpelóides e pentândricos.(AU)

Diallel mixed-model analysis of papaya fruit deformities / Análise dialélica via modelos mistos das deformidades em frutos de mamoeiro

Changes in the shape of fruits from hermaphrodite flowers due to floral anomalies are quite common. The aim of this study was to estimate the effects of the general and specific combining ability on the proportions of normal and deformed (carpelloid and pentandric) papaya fruits in hermaphrodite plants using mixed models. The JS12-N, Waimanalo and Golden parents showed the potential to decrease the number of deformed fruits. In contrast, the Maradol x JS12-N, Maradol x Waimanalo, Maradol x Golden, JS12-N x JS12-4, JS12-N x Sekati, JS12-N x Waimanalo, Waimanalo x JS12-N, Waimanalo x JS12-4, Waimanalo x Golden, Waimanalo x Sunrise Solo 72/12, Golden x Maradol, Golden x JS12-N, Golden x Sekati, Golden x Waimanalo and São Mateus x JS12-N hybrids have the potential to increase the number of marketable fruits while decreasing the number of carpelloid and pentandric fruits.

Alterações na forma dos frutos originários de flores hermafroditas são bastante comuns. Estas alterações são provocadas por anomalias florais. Este trabalho teve como objetivo obter via modelos mistos estimativas de capacidade geral e específica de combinação para proporção de frutos de mamoeiro normais e com deformidades: carpeloidia e pentandria, em plantas hermafroditas. Os genitores JS12-N, Waimanalo e Golden apresentam potencial para redução de frutos com deformidades. Os híbridos Maradol x JS12-N, Maradol x Waimanalo, Maradol x Golden, JS 12-N x JS12-4, JS 12-N x Sekati, JS 12-N x Waimanalo, Waimanalo x JS12-N, Waimanalo x JS12-4, Waimanalo x Golden, Waimanalo x Sunrise Solo 72/12, Golden x Maradol, Golden x JS12-N, Golden x Sekati, Golden x Waimanalo e São Mateus x JS12-N apresentaram potencial para aumento do número de frutos comerciais e redução de frutos carpelóides e pentândricos.

Diallel mixed-model analysis of papaya fruit deformities / Análise dialélica via modelos mistos das deformidades em frutos de mamoeiro

ABSTRACT: Changes in the shape of fruits from hermaphrodite flowers due to floral anomalies are quite common. The aim of this study was to estimate the effects of the general and specific combining ability on the proportions of normal and deformed (carpelloid and pentandric) papaya fruits in hermaphrodite plants using mixed models. The ‘JS12-N’, ‘Waimanalo’ and ‘Golden’ parents showed the potential to decrease the number of deformed fruits. In contrast, the ‘Maradol x JS12-N’, ‘Maradol x Waimanalo’, ‘Maradol x Golden’, ‘JS12-N x JS12-4’, ‘JS12-N x Sekati’, ‘JS12-N x Waimanalo’, ‘Waimanalo x JS12-N’, ‘Waimanalo x JS12-4’, ‘Waimanalo x Golden’, ‘Waimanalo x Sunrise Solo 72/12’, ‘Golden x Maradol’, ‘Golden x JS12-N’, ‘Golden x Sekati’, ‘Golden x Waimanalo’ and ‘São Mateus x JS12-N’ hybrids have the potential to increase the number of marketable fruits while decreasing the number of carpelloid and pentandric fruits.

RESUMO: Alterações na forma dos frutos originários de flores hermafroditas são bastante comuns. Estas alterações são provocadas por anomalias florais. Este trabalho teve como objetivo obter via modelos mistos estimativas de capacidade geral e específica de combinação para proporção de frutos de mamoeiro normais e com deformidades: carpeloidia e pentandria, em plantas hermafroditas. Os genitores ‘JS12-N’, ‘Waimanalo’ e ‘Golden’ apresentam potencial para redução de frutos com deformidades. Os híbridos ‘Maradol x JS12-N’, ‘Maradol x Waimanalo’, ‘Maradol x Golden’, ‘JS 12-N x JS12-4’, ‘JS 12-N x Sekati’, ‘JS 12-N x Waimanalo’, ‘Waimanalo x JS12-N’, ‘Waimanalo x JS12-4’, ‘Waimanalo x Golden’, ‘Waimanalo x Sunrise Solo 72/12’, ‘Golden x Maradol’, ‘Golden x JS12-N’, ‘Golden x Sekati’, ‘Golden x Waimanalo’ e ‘São Mateus x JS12-N’ apresentaram potencial para aumento do número de frutos comerciais e redução de frutos carpelóides e pentândricos.

Model-assisted phenotyping by digital images in papaya breeding program

Manual phenotyping for papaya Carica papaya (L) breeding purposes limits the evaluation of a great number of plants and hampers selection of superior genotypes. This study aimed to validate two methodologies for the phenotyping of morpho-agronomic plant traits using image analysis and fruit traits through image processing. In plants of the THB variety and UENF/Caliman-01 hybrid two images (A and B) were analyzed to estimate commercial and irregularly shaped fruits. Image A was also used in the estimation of plant height, stem diameter and the first fruit insertion height. In THB fruits, largest and smallest diameters, length, and volume were estimated by using a caliper and image processing (IP). Volume was obtained by water column displacement (WCD) and by the expression of ellipsoid approximation (EA). Correlations above 0.85 between manual and image measurements were obtained for all traits. The averages of the morpho-agronomic traits, estimated by using images, were similar when compared to the averages measured manually. In addition, the errors of the proposed methodologies were low compared to manual phenotyping. Bland-Altman's approach indicated agreement between the volume estimated by WCD and EA using caliper and IP. The strong association obtained between volume and fruit weight suggests the use of regression to estimate this trait. Thus, the expectation is that image-based phenotyping can be used to expand the experiments, thereby maintaining accuracy and providing greater genetic gains in the selection of superior genotypes.

Model-assisted phenotyping by digital images in papaya breeding program

Manual phenotyping for papaya Carica papaya (L) breeding purposes limits the evaluation of a great number of plants and hampers selection of superior genotypes. This study aimed to validate two methodologies for the phenotyping of morpho-agronomic plant traits using image analysis and fruit traits through image processing. In plants of the THB variety and UENF/Caliman-01 hybrid two images (A and B) were analyzed to estimate commercial and irregularly shaped fruits. Image A was also used in the estimation of plant height, stem diameter and the first fruit insertion height. In THB fruits, largest and smallest diameters, length, and volume were estimated by using a caliper and image processing (IP). Volume was obtained by water column displacement (WCD) and by the expression of ellipsoid approximation (EA). Correlations above 0.85 between manual and image measurements were obtained for all traits. The averages of the morpho-agronomic traits, estimated by using images, were similar when compared to the averages measured manually. In addition, the errors of the proposed methodologies were low compared to manual phenotyping. Bland-Altman's approach indicated agreement between the volume estimated by WCD and EA using caliper and IP. The strong association obtained between volume and fruit weight suggests the use of regression to estimate this trait. Thus, the expectation is that image-based phenotyping can be used to expand the experiments, thereby maintaining accuracy and providing greater genetic gains in the selection of superior genotypes.(AU)