RESUMO
Previous studies of color matching found that Grassmann's laws are not obeyed in the short-wavelength region when the method of maximum saturation matching is compared with Maxwell matching. The first experiment evaluated whether the discrepancy might be due to a discrimination matching range asymmetry around either the saturated or desaturated matches and concluded that asymmetry is not the dominant factor. The second and third experiments were designed to evaluate postreceptoral mechanisms. The results pointed to the conjunction of three factors as being the principal cause of the failures of Grassmann's laws: the spatial inhomogeneity of the macular pigment distribution, the spatially dissimilar L/M-and S-cone distributions, and a change in the weightings of postreceptoral mechanisms mediating S-cone chromatic and L/M-cone luminance discriminations.
Assuntos
Testes de Percepção de Cores , Percepção de Cores/fisiologia , Modelos Biológicos , Calibragem , Cor , Discriminação Psicológica/fisiologia , Humanos , Masculino , Estimulação Luminosa , Retina/fisiologiaRESUMO
This study investigated how acute alcohol intake affects contrast processing mediated by inferred magnocellular (MC) and parvocellular (PC) pathways. Achromatic contrast discrimination thresholds were measured in 16 young healthy participants using a steady-pedestal, pulsed-pedestal or pedestal-Δ-pedestal paradigm designed to favor the inferred MC or the PC pathway. Each participant completed two randomized sessions that included consumption of either 0.8 g/kg alcohol or a placebo beverage, with each session consisting of contrast discrimination measurements at baseline and at 60 min following beverage consumption. The results showed that, compared to placebo, alcohol significantly reduced MC contrast sensitivity and PC contrast gain but had no effect on PC contrast sensitivity for the majority of the participants; and did not alter MC contrast gain consistently across participants. The decrease in contrast gain in the PC pathway can be interpreted as a degradation of the postretinal signal-to-noise ratio, whereas the decrease of sensitivity in the MC pathway likely results from a change of cortical processing.
Assuntos
Defeitos da Visão Cromática/induzido quimicamente , Sensibilidades de Contraste/efeitos dos fármacos , Etanol/farmacologia , Vias Visuais/fisiologia , Adulto , Depressores do Sistema Nervoso Central/farmacologia , Defeitos da Visão Cromática/fisiopatologia , Feminino , Humanos , Masculino , Estimulação Luminosa/métodos , Psicofísica/métodos , Valores de Referência , Vias Visuais/efeitos dos fármacosRESUMO
Human vision starts with the activation of rod photoreceptors in dim light and short (S)-, medium (M)-, and long (L)- wavelength-sensitive cone photoreceptors in daylight. Recently a parallel, non-rod, non-cone photoreceptive pathway, arising from a population of retinal ganglion cells, was discovered in nocturnal rodents. These ganglion cells express the putative photopigment melanopsin and by signalling gross changes in light intensity serve the subconscious, 'non-image-forming' functions of circadian photoentrainment and pupil constriction. Here we show an anatomically distinct population of 'giant', melanopsin-expressing ganglion cells in the primate retina that, in addition to being intrinsically photosensitive, are strongly activated by rods and cones, and display a rare, S-Off, (L + M)-On type of colour-opponent receptive field. The intrinsic, rod and (L + M) cone-derived light responses combine in these giant cells to signal irradiance over the full dynamic range of human vision. In accordance with cone-based colour opponency, the giant cells project to the lateral geniculate nucleus, the thalamic relay to primary visual cortex. Thus, in the diurnal trichromatic primate, 'non-image-forming' and conventional 'image-forming' retinal pathways are merged, and the melanopsin-based signal might contribute to conscious visual perception.
Assuntos
Percepção de Cores/fisiologia , Macaca/fisiologia , Células Ganglionares da Retina/fisiologia , Opsinas de Bastonetes/metabolismo , Núcleos Talâmicos/fisiologia , Animais , Células Cultivadas , Escuridão , Humanos , Técnicas In Vitro , Luz , Transdução de Sinal Luminoso/efeitos da radiação , Retina/citologia , Retina/fisiologia , Retina/efeitos da radiação , Células Fotorreceptoras Retinianas Cones/fisiologia , Células Fotorreceptoras Retinianas Cones/efeitos da radiação , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/efeitos da radiação , Células Fotorreceptoras Retinianas Bastonetes/fisiologia , Células Fotorreceptoras Retinianas Bastonetes/efeitos da radiação , Opsinas de Bastonetes/genética , Núcleos Talâmicos/efeitos da radiação , Vias Visuais/fisiologia , Vias Visuais/efeitos da radiaçãoRESUMO
The purpose of this study was to evaluate the feasibility of measuring rod and cone electroretinograms (ERGs) at a single mesopic adaptation level. To accomplish this, a four-primary photostimulator was implemented using a commercially available ERG system (Diagnosys ColorDome) to generate three types of stimuli that temporally modulated rods alone, cones alone, and rods and cones simultaneously. For each stimulus type, ERGs were recorded as a function of temporal frequency (2, 4, 8, or 16 Hz) and mesopic light levels (0.02, 0.16, or 1.26 cd/m(2)) in normal observers and patients with retinitis pigmentosa (RP) or cone-rod degeneration. The normal observers ERG waveforms showed a clear periodic pattern, mirroring the sinusoidal stimuli. At all light levels, rod responses were always higher than cone responses for temporal frequencies between 2 and 8 Hz, suggesting that rods dominated the responses. Cone responses were minimal at the lowest light level and increased with increases in light level. The amplitude of the response to the combined stimuli was intermediate between that of the isolated cone and the isolated rod stimuli for all light levels. Good receptoral isolation was confirmed by the results showing (1) minimal or no rod ERGs but recordable cone ERGs in the patients and (2) high correlation between the ERG amplitudes obtained from the four-primary method and those from the ISCEV standard clinical protocol in normal observers.
Assuntos
Eletrorretinografia/métodos , Visão Mesópica/fisiologia , Células Fotorreceptoras de Vertebrados/fisiologia , Adaptação Ocular/fisiologia , Adolescente , Adulto , Idoso , Estudos de Viabilidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Degeneração Retiniana/fisiopatologia , Retinose Pigmentar/fisiopatologia , Adulto JovemRESUMO
In the mid-1990s, the Pokorny and Smith research group began a series of psychophysical experiments with the aim of separately measuring magnocellular (MC)- and parvocellular (PC)-pathway mediated achromatic contrast discrimination. Three paradigms provide complementary information: The pulsed-pedestal paradigm reveals PC contrast gain, the steady-pedestal paradigm reveals steady-state MC-pathway sensitivity, and the pedestal-Δ-pedestal paradigm reveals MC contrast gain. Further studies investigated the temporal and spatial summation properties of the underlying mechanisms and extended the work to include measures of spatial resolution, chromatic contrast discrimination, the detection and identification of stimulus polarity, and the inferred retinal mechanisms mediating illusory distortions. Other laboratories have also applied the methods to the study of normal and clinically impaired vision. This review describes the pedestal methodologies, how they relate to physiology, and how they have been and should be employed.
Assuntos
Sensibilidades de Contraste , Discriminação Psicológica/fisiologia , Retina/fisiologia , Vias Visuais/fisiologia , Humanos , Estimulação Luminosa , PsicofísicaRESUMO
Contrast gain reflects the rapidity of response amplitude increase with increase in stimulus contrast. In physiology, contrast gain can be measured directly as the initial slope of cell contrast response function. In psychophysics, contrast gain estimation is not straightforward. Further, rod and cone contrast gains have not been measured psychophysically at mesopic light levels where both rods and cones are active, due to the difficulty in producing stimuli that excite rods and cones separately at the same adaptation level. Here, we estimated rod and contrast gains by fitting reaction time distributions measured at a light level in which rods alone (scotopic), rods and cones (mesopic), or cones alone (photopic) mediate vision. The reaction time distributions were modeled by two different strategies, a simplified diffusion model that assumes a stochastic accumulation process and a model we developed that begins with sensory input based on early visual processing impulse response functions and assumes the reaction time variability originates in the response criterion. Estimates of contrast gain from both models were comparable and consistent with primate physiology measurements.
Assuntos
Sensibilidades de Contraste/fisiologia , Modelos Neurológicos , Células Fotorreceptoras Retinianas Cones/fisiologia , Células Fotorreceptoras Retinianas Bastonetes/fisiologia , Adaptação Ocular/fisiologia , Animais , Visão de Cores/fisiologia , Adaptação à Escuridão/fisiologia , Estimulação Luminosa/métodos , Primatas , Psicofísica , Tempo de Reação/fisiologia , Limiar Sensorial/fisiologiaRESUMO
We as a couple spent 50 years working in visual psychophysics of color vision, temporal vision, and luminance adaptation. We sought collaborations with ophthalmologists, anatomists, physiologists, physicists, and psychologists, aiming to relate visual psychophysics to the underlying physiology of the primate retina. This review describes our journey and reflections in exploring the visual system.
Assuntos
Oftalmologia/história , História do Século XX , História do Século XXI , Visão OcularRESUMO
Signals from rods can alter chromatic discrimination. Here, chromatic discrimination ellipses were determined in the presence of rod incremental and decremental pedestals at mesopic light levels. The data were represented in a relative cone Troland space, normalized by discrimination thresholds measured along the cardinal axes without a rod pedestal. In the quadrant of cone space where L-cone relative to M-cone excitation increased, and S-cone excitation decreased, rod incremental pedestals degraded chromatic discrimination, and rod decremental pedestals improved chromatic discrimination. Discrimination in the other three quadrants of cone space was unaffected by the incremental or decremental rod pedestals. A second experiment measured chromatic discrimination under conditions where cone pedestals were matched to the appearances of the incremental and decremental rod pedestals. Based on the matching pedestal data, discrimination then could be measured independently along the cardinal axes using either chromatic [L/(L + M); S/(L + M)] or luminance (L + M) pedestal components. The discrimination data altered by the rod pedestals were similar to chromatic cone pedestals for L/M increment discrimination, but similar to luminance cone pedestals for S decrement discrimination. The results indicated that the rod and cone signals combined differently in determining chromatic discrimination for different post-receptoral pathways.
Assuntos
Percepção de Cores/fisiologia , Discriminação Psicológica/fisiologia , Células Fotorreceptoras Retinianas Bastonetes/fisiologia , Calibragem , Comportamento de Escolha , Fixação Ocular , Humanos , Luz , Masculino , Valores de Referência , Células Fotorreceptoras Retinianas Cones/fisiologiaRESUMO
In the natural environment, color discriminations are made within a rich context of spatial and temporal variation. In classical laboratory methods for studying chromatic discrimination, there is typically a border between the test and adapting fields that introduces a spatial chromatic contrast signal. Typically, the roles of spatial and temporal contrast on chromatic discrimination are not assessed in the laboratory approach. In this study, S-cone discrimination was measured using stimulus paradigms that controlled the level of spatio-temporal S-cone contrast between the tests and adapting fields. The results indicate that S-cone discrimination of chromaticity differences between a pedestal and adapting surround is equivalent for stimuli containing spatial, temporal or spatial-and-temporal chromatic contrast between the test field and the surround. For a stimulus condition that did not contain spatial or temporal contrast, the visual system adapted to the pedestal instead of the surround. The data are interpreted in terms of a model consistent with primate koniocellular pathway physiology. The paradigms provide an approach for studying the effects of spatial and temporal contrast on discrimination in natural scenes.
Assuntos
Discriminação Psicológica/fisiologia , Células Fotorreceptoras Retinianas Cones/fisiologia , Limiar Sensorial/fisiologia , Adulto , Animais , Percepção de Cores , Sensibilidades de Contraste , Humanos , Primatas , Percepção Espacial/fisiologia , Especificidade da EspécieRESUMO
People with normal trichromatic color vision experience variegated hue percepts under dim illuminations where only rod photoreceptors mediate vision. Here, hue perceptions were determined for persons with congenital color vision deficiencies over a wide range of light levels, including very low light levels where rods alone mediate vision. Deuteranomalous trichromats, deuteranopes and protanopes served as observers. The appearances of 24 paper color samples from the OSA Uniform Color Scales were gauged under successively dimmer illuminations from 10 to 0.0003 Lux (1.0 to -3.5 log Lux). Triads of samples were chosen representing each of eight basic color categories; "red," "pink," "orange," "yellow," "green," "blue," "purple," and "gray." Samples within each triad varied in lightness. Observers sorted samples into groups that they could categorize with specific color names. Above -0.5 log Lux, the dichromatic and anomalous trichromatic observers sorted the samples into the original representative color groups, with some exceptions. At light levels where rods alone mediate vision, the color names assigned by the deuteranomalous trichromats were similar to the color names used by color normals; higher scotopic reflectance samples were classified as blue-green-grey and lower reflectance samples as red-orange. Color names reported by the dichromats at the dimmest light levels had extensive overlap in their sample scotopic lightness distributions. Dichromats did not assign scotopic color names based on the sample scotopic lightness, as did deuteranomalous trichromats and colour-normals. We reasoned that the reduction in color gamut that a dichromat experiences at photopic light levels leads to a limited association of rod color perception with objects differing in scotopic reflectance.
Assuntos
Percepção de Cores/fisiologia , Defeitos da Visão Cromática/fisiopatologia , Escuridão , Luz , Estudos de Coortes , Defeitos da Visão Cromática/classificação , Humanos , Iluminação , Acuidade VisualRESUMO
PURPOSE: To measure achromatic spatial contrast sensitivity in patients with anisometropic amblyopia under conditions favoring inferred parvocellular (PC) or magnocellular (MC) pathway mediation. METHODS: Fourteen anisometropic amblyopes (VA amblyopic eye 6/12 or lower; better eye greater than 6/7.5) and 10 age-matched, nonamplyopic controls (VA 6/6) participated. Foveal spatial contrast sensitivity was measured using localized, spatially narrow band targets (0.25-8.0 cpd) presented in the center of a steady pedestal (favoring MC detection) or a pulsed pedestal (favoring PC detection) that was set within a uniform surround. RESULTS: Spatial contrast sensitivity functions were bandpass for the steady-pedestal and lowpass for the pulsed-pedestal. Under steady-pedestal adaptation, the amblyopes showed reduced spatial contrast sensitivity at intermediate frequencies (1-2 cpd), consistent with MC sensitivity loss. For the pulsed-pedestal condition, a generalized loss of sensitivity was observed across all spatial frequencies (0.5-4 cpd), consistent with PC sensitivity loss. The magnitudes of inferred MC and PC loss were similar. In the steady- and pulsed-pedestal paradigms, results for the better eye of greater than 75% of the amblyopes were normal or near normal at low and intermediate spatial frequencies. CONCLUSIONS: Anisometropic amblyopia produces spatial contrast sensitivity losses in inferred PC- and MC-mediated vision, suggesting there may be anomalous processing of MC and PC signals in higher visual areas, including those with orientation and spatial frequency selective cells in the visual cortex. With spatially localized stimuli and a paradigm designed to distinguish between MC and PC vision under conditions that differ only in the interstimulus adaptation, the better eye of the amblyopes was normal or near normal.
Assuntos
Ambliopia/fisiopatologia , Sensibilidades de Contraste , Percepção Espacial , Vias Visuais/fisiologia , Adolescente , Adulto , Criança , Feminino , Fóvea Central/fisiologia , Humanos , Masculino , Orientação/fisiologia , Estimulação Luminosa , PsicofísicaRESUMO
Reaction times for incremental and decremental stimuli were measured at five suprathreshold contrasts for six retinal illuminance levels where rods alone (0.002-0.2 Trolands), rods and cones (2-20 Trolands) or cones alone (200 Trolands) mediated detection. A 4-primary photostimulator allowed independent control of rod or cone excitations. This is the first report of reaction times to isolated rod or cone stimuli at mesopic light levels under the same adaptation conditions. The main findings are: (1) For rods, responses to decrements were faster than increments, but cone reaction times were closely similar. (2) At light levels where both systems were functional, rod reaction times were approximately 20 ms longer. The data were fitted with a computational model that incorporates rod and cone impulse response functions and a stimulus-dependent neural sensory component that triggers a motor response. Rod and cone impulse response functions were derived from published psychophysical two-pulse threshold data and temporal modulation transfer functions. The model fits were accomplished with a limited number of free parameters: two global parameters to estimate the irreducible minimum reaction time for each receptor type, and one local parameter for each reaction time versus contrast function. This is the first model to provide a neural basis for the variation in reaction time with retinal illuminance, stimulus contrast, stimulus polarity, and receptor class modulated.
Assuntos
Simulação por Computador , Modelos Psicológicos , Tempo de Reação/fisiologia , Células Fotorreceptoras Retinianas Cones/fisiologia , Células Fotorreceptoras Retinianas Bastonetes/fisiologia , Percepção Visual/fisiologia , Adaptação Ocular , Sensibilidades de Contraste , Humanos , Estimulação Luminosa , PsicofísicaRESUMO
PURPOSE: To compare reaction time (RT) to rod incremental and decremental stimuli expressed in physical contrast units or psychophysical threshold units. METHODS: Rod contrast detection thresholds and suprathreshold RTs were measured for Rapid-On and Rapid-Off ramp stimuli. RESULTS: Threshold sensitivity to Rapid-Off stimuli was higher than to Rapid-On stimuli. Suprathreshold RTs specified in Weber contrast for Rapid-Off stimuli were shorter than for Rapid-On stimuli. Reaction time data expressed in multiples of threshold reversed the outcomes: Reaction times for Rapid-On stimuli were shorter than those for Rapid-Off stimuli. The use of alternative contrast metrics also failed to equate RTs. CONCLUSIONS: A case is made that the interpretation of RT data may be confounded when expressed in threshold units. Stimulus energy or contrast is the only metric common to the response characteristics of the cells underlying speeded responses. The use of threshold metrics for RT can confuse the interpretation of an underlying physiological process.
Assuntos
Sensibilidades de Contraste/fisiologia , Células Fotorreceptoras Retinianas Bastonetes/fisiologia , Humanos , Masculino , Estimulação Luminosa/métodos , Psicofísica , Tempo de Reação , Limiar Sensorial/fisiologiaRESUMO
Melanopsin, a novel photopigment, has recently been localized to a population of retinal ganglion cells that display inherent photosensitivity. During continuous light and following light offset, primates are known to exhibit sustained pupilloconstriction responses that resemble closely the photoresponses of intrinsically-photoreceptive ganglion cells. We report that, in the behaving macaque, following pharmacological blockade of conventional photoreceptor signals, significant pupillary responses persist during continuous light and following light offset. These pupil responses display the unique spectral tuning, slow kinetics, and irradiance coding of the sustained, melanopsin-derived ganglion cell photoresponses. We extended our observations to humans by using the sustained pupil response following light offset to document the contribution of these novel ganglion cells to human pupillary responses. Our results indicate that the intrinsic photoresponses of intrinsically-photoreceptive retinal ganglion cells play an important role in the pupillary light reflex and are primarily responsible for the sustained pupilloconstriction that occurs following light offset.
Assuntos
Reflexo Pupilar/fisiologia , Células Ganglionares da Retina/fisiologia , Opsinas de Bastonetes/fisiologia , Animais , Eletrorretinografia/métodos , Humanos , Luz , Transdução de Sinal Luminoso/fisiologia , Macaca mulatta , Células Fotorreceptoras de Vertebrados/efeitos dos fármacos , Células Fotorreceptoras de Vertebrados/fisiologia , Células Fotorreceptoras Retinianas Cones/efeitos dos fármacos , Células Fotorreceptoras Retinianas Cones/fisiologia , Células Fotorreceptoras Retinianas Bastonetes/efeitos dos fármacos , Células Fotorreceptoras Retinianas Bastonetes/fisiologiaRESUMO
PURPOSE: Imaging studies from anesthetized feline, primate, and human retinas have revealed near-infrared fundus reflectance changes induced by visible light stimulation. In the present study, the spatial and temporal properties of similar changes were characterized in normal, awake humans. METHODS: Five normal human subjects were studied. A modified fundus camera was used to image changes in retinal reflectance of 780-nm near-infrared light imaged onto a 12-bit charge-coupled device (CCD) camera in response to a green (540 nm) visual stimulus. During 60 seconds of recording (frame rate, 3 Hz) 10 cycles were recorded, during each of which 3 seconds of blank and then 3 seconds of either vertical bar or blank stimulus was projected. The change in the average near-infrared reflectance of the stimulated retinal region relative to an equal-sized nonstimulated region (r is the ratio of reflectance between the two retinal areas) was analyzed with a mixed model for repeated measures. RESULTS: The mixed model showed a significant average decrease in r of 0.14% (95% CI, -0.25 to -0.03) over all subjects induced by bar stimulus cycles, with a gradual return to baseline after stimulus offset, compared with only a 0.04% (95% CI, -0.11-+0.20) decrease in r induced by blank, nonstimulated cycles. The mixed model for individuals showed a decreasing linear trend in r over time during bar stimulation, but no decrease for blank cycles in three of five subjects. CONCLUSIONS: There was a localized decrease in reflectance in response to 780-nm near-infrared light in the retinal region exposed to a visual stimulus, which was significant in three of five subjects. It is presumed that the reflectance change represents the functional activity of the retina in response to a visual stimulus.
Assuntos
Fundo de Olho , Raios Infravermelhos , Retina/fisiologia , Retina/efeitos da radiação , Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estimulação Luminosa , FotografaçãoRESUMO
The design objective was to develop an inexpensive digital to analog (D/A) converter for use in vision science. Soundcards are hardware units that can be integral or can be added to a computer to add sound capability. A soundcard contains D/A converters designed to work in the audio frequency range, typically 20-20,000 Hz. Soundcard outputs are high-pass filtered and thus do not convey sub-audio frequency or dc information. It is possible to circumvent this design feature by programming the desired output waveform as an amplitude modulation of a high frequency carrier, and then demodulating the soundcard output. The circuit, using a 20 kHz carrier, provides precise D/A conversion for the frequency range relevant for vision experiments, dc to 100 Hz, using inexpensive readily available components. The specific application was for 8 channels of D/A conversion using a Macintosh computer running under OS X. The software needed to program stimuli was created using CoreAudio, a library for programming sounds in OS X. Using soundcards on other platforms would not be a problem, as long as there exists a low level library that would enable the wave table to be filled.
Assuntos
Periféricos de Computador/normas , Eletrofisiologia/instrumentação , Neurofisiologia/instrumentação , Estimulação Luminosa/instrumentação , Processamento de Sinais Assistido por Computador/instrumentação , Encéfalo/fisiologia , Periféricos de Computador/economia , Eletrônica/instrumentação , Eletrônica/métodos , Eletrofisiologia/métodos , Neurofisiologia/métodos , Estimulação Luminosa/métodos , Software/normas , Software/tendências , Som , Percepção Visual/fisiologiaRESUMO
Traditional methods for studying the effects of rod activity on color vision make it hard to assess the underlying physiological mechanisms. In this study, rod-mediated changes in color appearance were assessed by matching them with cone-mediated color changes. A four-primary photostimulator allowed independent control of rod and cone stimulation and identification of the cone types that generate color sensations equivalent to rod color sensations. The results showed that increases in rod stimulation required matches with cone stimuli that excited M-cones more than L-cones for all conditions. Matches for low-luminance conditions also required some S-cone stimulation. A subsidiary experiment showed that increases in rod modulation of an inducing field produced chromatic contrast effects like those produced by the M-cone system. The data are consistent with a hypothesis of perceptual normalization of scotopic vision to the chromatic appearance of objects under photopic conditions.
Assuntos
Células Fotorreceptoras Retinianas Cones/fisiologia , Células Fotorreceptoras Retinianas Bastonetes/fisiologia , Percepção de Cores/fisiologia , Colorimetria/instrumentação , Adaptação à Escuridão , Humanos , Estimulação Luminosa , PsicofísicaRESUMO
A cone chromaticity space, a transform of a colorimetric specification system into coordinates that represent cone excitations, does not provide color appearance information. Boynton and Olson (Color Research and Application 12, 94-105, 1987) gathered color naming for the 424 Optical Society of America Uniform Color Scales (OSA-UCS) color samples. Here, a computational algorithm was developed that converts OSA-UCS sample values into L, M, S cone excitations based on the 1964 CIE 10 degrees Standard Observer. This makes it possible to plot the cone chromaticities associated with the eight color names used by Boynton and Olsen's observers to describe the non-dark appearing colors.
Assuntos
Testes de Percepção de Cores/métodos , Percepção de Cores/fisiologia , Células Fotorreceptoras Retinianas Cones/fisiologia , Algoritmos , Discriminação Psicológica , Humanos , Estimulação Luminosa/métodos , PsicofísicaRESUMO
PURPOSE: Anatomical and physiological studies show that in primates, visual information is conveyed through two parallel pathways, including the magnocellular (MC-) and parvocellular (PC-) pathways. However, the functional separation between the two pathways remains controversial and challenging. To resolve this, we show a psychophysical approach to desensitize the inferred MC-pathway of human observers independently of the inferred PC-pathway. METHODS: The steady-pedestal and pulsed-pedestal paradigms that allow detection and discrimination to be mediated by only the inferred MC- or PC-pathway were used. Three observers (one male, aged 43 years, and two females, aged 33 and 62 years) adapted to either a steadily presented pedestal or a 2- or 10-Hz 50% contrast square-wave modulated luminance flicker. Contrast discrimination thresholds were measured following the flicker adaptation. RESULTS: Flicker adaptation reduces contrast detection and discrimination of the MC-pathway but not the PC-pathway, with larger MC losses from 10-Hz (â¼ 0.28 log unit loss, P < 0.05 for all observers) than 2-Hz flicker (â¼ 0.13 log unit loss, P < 0.05 for one or two observers depending on stimulus size). Further, our results show that the PC-pathway does not mediate the contrast detection threshold at the background luminance following MC-pathway desensitization. CONCLUSIONS: This study demonstrates the feasibility of independently manipulating sensitivity of the MC-pathway in human observers. Our paradigms provide powerful tools to independently investigate the perceptual functions in the MC- and PC-pathways. This could lead to a better understanding of the perceptual functions of these pathways.
Assuntos
Adaptação Fisiológica/fisiologia , Vias Visuais/fisiologia , Adulto , Sensibilidades de Contraste/fisiologia , Discriminação Psicológica/fisiologia , Feminino , Corpos Geniculados/citologia , Corpos Geniculados/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Estimulação Luminosa/métodos , Psicofísica , Limiar Sensorial/fisiologiaRESUMO
PURPOSE: To define the contrast sensitivity deficits of patients with retinitis pigmentosa (RP) under testing conditions designed to emphasize threshold mediation by either the magnocellular (MC) or parvocellular (PC) pathway. METHOD: Contrast sensitivity was measured with spatially localized, narrow-band test patterns at peak spatial frequencies ranging from 0.25 to 8 cycles per degree (cpd), using a steady-pedestal paradigm (brief presentation of the test stimulus against a continuously presented luminance pedestal) and a pulsed-pedestal paradigm (simultaneous brief presentation of the test stimulus and luminance pedestal) to favor the MC and PC pathways, respectively. The contrast sensitivity functions of 12 patients with RP who had visual acuities ranging between 20/12.5 and 20/40 were compared to those of 10 visually normal, age-equivalent control observers. RESULTS: Five of the patients with RP who had Snellen visual acuities better than 20/25 had contrast sensitivity functions that were within the normal limits at all spatial frequencies for both testing paradigms. The other seven patients with RP had reduced contrast sensitivities for both paradigms, with the greatest reduction in sensitivity occurring at the highest spatial frequency. Their contrast sensitivity deficits were equivalent for the steady- and pulsed-pedestal paradigms. CONCLUSIONS: As observed in previous studies, the degree of contrast sensitivity loss shown by the patients with RP was greatest at the highest stimulus spatial frequency. However, in comparison to prior studies of contrast discrimination in patients with RP, there was no evidence of a preferential contrast sensitivity loss within the MC pathway. This apparent discrepancy is attributed to differences in the test targets and psychophysical judgments that were used in the studies, which emphasizes the importance of task characteristics in evaluating relative deficits within the MC and PC processing streams in visual disorders.