RESUMO
A cell line, TCCSUP, derived from an undifferentiated, Grade IV transitional cell carcinoma is described. The karyotype showed an abnormal distribution of chromosomes, with no obvious modal number. Distinct marker chromosomes were observed in both early and late in vitro passages. These cells have been subcultured over 50 times during a 20-month period. TCCSUP differs in certain morphological and immunological features from other cell lines from transitional cell carcinomas.
Assuntos
Carcinoma de Células de Transição/genética , Linhagem Celular , Neoplasias da Bexiga Urinária/genética , Carcinoma de Células de Transição/imunologia , Carcinoma de Células de Transição/ultraestrutura , Testes Imunológicos de Citotoxicidade , Humanos , Cariotipagem , Linfócitos/imunologia , Microscopia Eletrônica , Neoplasias da Bexiga Urinária/imunologia , Neoplasias da Bexiga Urinária/ultraestruturaRESUMO
A cell line (J82) was derived from a poorly differentiated, invasive, transitional-cell carcinoma, Stage T3. The cells have been propagated in vitro for 5 years and showed 100% aneuploidy and a mixed epithelial-fibroblastic morphology. The majority of cells contained 2Y chromosomes and several distinctive markers. Peripheral-blood lymphocytes from the donor of the J82 cells were tested sequentially for cytotoxicity toward autologous and allogeneic tumour cells. Autologous cytotoxicity was detected against J82 cells in early in vitro passage. Allogeneic lymphocytes from some patients with transitional-cell carcinoma were also cytotoxic to J82 cells in primary culture. However, selective cytotoxicity by lymphoid cells from bladder-carcinoma patients was not detected against J82 cells in long-term tissue culture.