Potential of biological sulphur recovery from thiosulphate by haloalkaliphilic Thioalkalivibrio denitrificans.

The aim of this study was to investigate the potential for elemental sulphur recovery from sulphurous solutions under aerobic and anoxic conditions by haloalkalophilic Thioalkalivibrio denitrificans at 0.8-19.6 g S2O32--S L-1 and 0.2-0.58 g NO2 L-1, respectively. The experiments were conducted as batch assays with haloalkaline (pH 10 and ≥ 14 g Na+ L-1) thiosulphate solution. Aerobically, the highest biotransformation rate of thiosulphate obtained was 0.03 h-1 at 8.5 g L S2O32--S. Based on Monod model, the maximum substrate utilisation rate (qm) was 0.024 h-1 with half saturation constant (Ks) 0.42 g S2O32--S L-1 at initial [S2O32--S] of 14 g L-1. S0 accumulated at [S2O32--S] ≥ 1.5 g L-1 (10% yield at initial 9.5 g S2O32--S L-1) and the highest S0 yield estimated with the model was 61% with initial [S2O32--S] of 16.5 g L-1. Anoxically, the maximum nitrite removal rate based on Monod modelling was 0.011 h-1 with Ks = 0.84 g NO2- L-1. Aerobically and anoxically the maximum specific growth rates (µm) were 0.046 and 0.022 h-1, respectively. In summary, high-rate aerobic biotransformation kinetics of thiosulphate were demonstrated, whereas the rates were slower and no S0 accumulated under anoxic conditions. Thus, future developments of biotechnical applications for the recovery of S0 from haloalkaline streams from the process industry should focus on aerobic treatment.HighlightsHaloalkaline S2O32- biotransformations kinetics by Thioalkalivibrio denitrificansAerobic thiosulphate-S bioconversion up to 0.024 h-1 with Ks = 0.42 g S2O32--S L-110% S0 yield with initial 9.5 g S2O32--S L-1 in aerobic conditionAnoxic NO2 removal up to 0.01 h-1 with Ks = 0.84 g NO2- L-1.

Effects of inorganic ions on autotrophic denitrification by Thiobacillus denitrificans and on heterotrophic denitrification by an enrichment culture.

Salinity of nitrate-laden wastewaters, such as those produced by metal industries, tanneries, and wet flue gas cleaning systems may affect their treatment by denitrification. Salt inhibition of denitrification has been reported, while impacts of individual ions remain poorly understood whilst being relevant for wastewaters where often the concentration of a single ion rather than the salts varies. The aim of this study was to determine the inhibition by inorganic ions (Na+, Cl-, SO42- and K+) commonly present in saline wastewaters on denitrification and reveal its potential for the treatment of such waste streams, like those produced by NOx-SOx removal scrubbers. The inhibitory effects were investigated for both heterotrophic (enrichment culture) and autotrophic (T. denitrificans) denitrification in batch assays, by using NaCl, Na2SO4, KCl and K2SO4 salts at increasing concentrations. The half inhibition concentrations (IC50) of Na+ (as NaCl), Na+ (as Na2SO4) and Cl- (as KCl) were: 4.3 ± 0.3, 7.9 ± 0.5 and 5.2 ± 0.3 g/L for heterotrophic, and 1-2.5, 2.5-5 and 4.1 ± 0.3 g/L for autotrophic denitrification, respectively. Heterotrophic denitrification was completely inhibited at 20 g/L Na+ (as NaCl), 30 g/L Na+ (as Na2SO4) and 30 g/L Cl- (as KCl), while autotrophic at 8 g/L Na+ (as NaCl), 10 g/L Na+ (as Na2SO4) and 15 g/L Cl- (as KCl). In both cases, Cl- addition had the most important role in decreasing denitrification rate, while Na+ at 1 g/L stimulated autotrophic denitrification but rapidly inhibited the rate at higher concentrations. Nitrite reduction was less inhibited by the ions than nitrate reduction and both the osmotic pressure and the toxicity of the single ions played key roles in the overall inhibition of denitrification. Eventually, both autotrophic and heterotrophic denitrification showed potential for the treatment of a saline wastewater from a NOx-SO2 removal scrubber from a pulp mill.

Removal of arsenic from acidic liquors using chemical and autotrophic and mixed heterotrophic bacteria-produced biogenic schwertmannites.

Arsenic penetrates human society through a variety of geological and anthropogenic processes, posing significant health hazards. Acid mine drainage, which contains high concentrations of heavy metals and sulfate, is formed by the biological oxidation of pyrite and other metal-containing sulfidic minerals and is a significant environmental hazard. Adsorption is a simple and effective method for removing arsenic from water. In this study, co-precipitation and adsorption of arsenic with biogenic and chemically produced iron-containing settleable precipitates, i.e. schwertmannites were studied. Autotrophic Leptospirillum ferrooxidans and heterotrophic mixed culture of Alicyclobacillus tolerans and Acidiphilium cryptum oxidized iron at rates from 18 to 23 mg/(L.h) in the presence of 5 and 10 mg/L As3+, and both cultures tolerated up to 100 mg As3+/L although Fe2+ oxidation rates decreased to 3-4 mg/(L.h). At Fe/As ratios of ≥20, As removal efficiencies of ≥95% were obtained by co-precipitation with Fe3+ at pH 3.5-4.5. Because schwertmannite precipitates produced by the heterotrophic culture formed crystals, it was studied for adsorptive removals of As3+ and As5+ and compared with chemically synthesized schwertmannites. As3+ (100 mg/L) adsorption onto biogenic and chemical schwertmannite were 25 and 44%, respectively, at pH 4. At 100 mg As5+/L, adsorption capacity and efficiency onto biogenic schwertmannite were 47 mg/g and 50%, respectively. At 300 mg As5+/L, adsorption capacity and efficiency onto chemical schwertmannite were 169 mg/g and 56%, respectively. In summary, biogenic schwertmannite has potential for As removal via co-precipitation with Fe3+ at pH 3.5-4.5 and Fe/As ratios of ≥20 due to low production cost from acidic mine drainage. In contrast to the schwertmannite generation methods, which are usually performed with autotrophic acidophilic bacteria in the literature, this efficient and modular schwertmannite production process and its evaluation on arsenic adsorption is an important potential in acidic mine drainage treatment containing arsenic.

Growth of Dunaliella tertiolecta and associated bacteria in photobioreactors.

The aim of this study was to test three flat-plate photobioreactor configurations for cultivation of marine green alga Dunaliella tertiolecta under non-axenic growth conditions and to characterize and quantify the associated bacteria. The photobioreactor cultivations were conducted using tap water-based media. Static mixers intended to enhance mixing and light utilization did not generally increase algal growth at the low light intensities used. The maximum biomass concentration (measured as volatile suspended solids) and maximum specific growth rate achieved in the flat plate with no mixer were 2.9 g l⁻¹ and 1.3 day⁻¹, respectively. Based on quantitative polymerase chain reaction, bacterial growth followed the growth of D. tertiolecta. Based on 16S rDNA amplification and denaturing gradient gel electrophoresis profiling, heterotrophic bacteria in the D. tertiolecta cultures mainly originated from the non-axenic algal inocula, and tap water heterotrophs were not enriched in high chloride media (3 % salinity). Bacterial communities were relatively stable and reproducible in all flat-plate cultivations and were dominated by Gammaproteobacteria, Flavobacteria, and Alphaproteobacteria.

Leaching of rare earth elements and base metals from spent NiMH batteries using gluconate and its potential bio-oxidation products.

Gluconate is known to mediate metal leaching. However, during bioleaching by e.g., Gluconobacter oxydans, gluconate can be oxidized to 2-ketogluconate and 5-ketogluconate. The impact of bio-oxidation of gluconate on metal leaching has not been investigated. Therefore, the aim of this study was to investigate leaching of rare earth elements (REEs) and base metals from spent nickel-metal-hydride (NiMH) batteries using gluconate, 2-ketogluconate and 5-ketogluconate. Batch leaching assays were conducted under controlled and uncontrolled pH conditions for 14 days using 60 mM of either the individual leaching agents or their various combinations. At target pH of 6.0 ± 0.1 and 9.0 ± 0.1 and without pH control, complexolysis was the dominating leaching mechanism and higher REE leaching efficiency was obtained with gluconate, while 5-ketogluconate enabled more efficient base metal leaching. At target pH of 3.0 ± 0.1, acidolysis dominated, and the base metal and REE leaching yields with all the tested leaching agents were higher than under the other studied pH conditions. The highest base metal and REE leaching yields (%) were obtained using gluconate at target pH of 3.0 ± 0.1 being 100.0 Mn, 90.3 Fe, 89.5 Co, 58.5 Ni, 24.0 Cu, 29.3 Zn and 56.1 total REEs. The obtained results are useful in optimization of heterotrophic bioleaching.

Simulated acid mine drainage treatment in iron oxidizing ceramic membrane bioreactor with subsequent co-precipitation of iron and arsenic.

Acid mine drainage (AMD), generated in the active and abandoned mine sites, is characterized by low pH and high metal concentrations. One AMD treatment possibility is biologically oxidizing Fe2+ followed by precipitation through pH control. As compared to autotrophic iron oxidizing microbial community, a microbial community enriched in the presence of organic nutrients was hypothesized to yield higher biomass during commissioning the bioreactor. In this study, the treatment of Fe, Cu, Co, Mn, Zn, Ni, and As containing simulated AMD was studied using an iron-oxidizing ceramic membrane bioreactor (CMBR) at varying hydraulic retention times (HRTs) (6-24 h) and two different feed Fe2+ concentrations (250 and 750 mg/L). The impact of tryptone soya broth (TSB) on the CMBR performance was also investigated. Almost complete Fe2+ oxidation and sustainable flux at around 5.0 L/(m2.h) were obtained in the CMBR with the Alicyclobacillus tolerans and Acidiphilium cryptum dominated enrichment culture. The Fe2+ oxidation rate, as assessed in batch operation cycles of CMBR, increased significantly with increasing Fe2+ loading to the bioreactor. The iron oxidation rate decreased by the elimination of organic matter from the feed. The increase of the CMBR permeate pH to 3.5-4.0 resulted in selective co-precipitation of As and Fe (over 99%) with the generation of biogenic schwertmannite.

Increase in sedimentary organic carbon with a change from hypoxic to oxic conditions.

In the Seto Inland Sea, Japan, chemical oxygen demand has increased over recent decades, while average dissolved oxygen concentrations in the bottom water have increased. In this study, we investigated responses of organic carbon (OC) in hypoxic sediment to changes of redox conditions using experimental columns containing sediment and overlying water. Surface sediment showed an increase in OC along with the change to an aerobic condition. Microbial community analysis showed a predominance of sulfur-oxidizing bacteria (SOB) such as Sulfurovum sp. in the sediment. This dominance could account for the increased OC. Additionally, the dissolved organic carbon (DOC) concentration in the overlying water increased. Further experiments using sandy sediment showed that biodegradation of Sulfurimonas denitrificans was associated with DOC release. These results show that a change in the sedimentary environment (increase in dissolved oxygen) increased the sedimentary OC and DOC of overlying water by stimulating certain autotrophic bacteria, especially the SOB.

Predictive modelling of Fe(III) precipitation in iron removal process for bioleaching circuits.

In this study, the applicability of three modelling approaches was determined in an effort to describe complex relationships between process parameters and to predict the performance of an integrated process, which consisted of a fluidized bed bioreactor for Fe(3+) regeneration and a gravity settler for precipitative iron removal. Self-organizing maps were used to visually evaluate the associations between variables prior to the comparison of two different modelling methods, the multiple regression modelling and artificial neural network (ANN) modelling, for predicting Fe(III) precipitation. With the ANN model, an excellent match between the predicted and measured data was obtained (R (2) = 0.97). The best-fitting regression model also gave a good fit (R (2) = 0.87). This study demonstrates that ANNs and regression models are robust tools for predicting iron precipitation in the integrated process and can thus be used in the management of such systems.

Hydrogenotrophic sulfate reduction in a gas-lift bioreactor operated at 9 degrees C.

The viability of low temperature sulfate reduction with hydrogen as electron donor was studied with a bench-scale gas-lift bioreactor (GLB) operated at 9 degrees C. Prior to the GLB experiment, the temperature range of sulfate reduction of the inoculum was assayed. The results of the temperature gradient assay indicated that the inoculum was a psychrotolerant mesophilic enrichment culture that had an optimal temperature for sulfate reduction of 31 degrees C, and minimum and maximum temperatures of 7 degrees C and 41 degrees C, respectively. In the GLB experiment at 9 degrees C, a sulfate reduction rate of 500-600 mg L(-1) d(-1), corresponding to a specific activity of 173 mg SO(4)(2-) g VSS(-1) d(-1), was obtained. The electron flow from the consumed H(2)-gas to sulfate reduction varied between 27% and 52%, while the electron flow to acetate production decreased steadily from 15% to 5%. No methane was produced. Acetate was produced from CO(2) and H(2) by homoacetogenic bacteria. Acetate supported the growth of some heterotrophic sulfate-reducing bacteria. The sulfate reduction rate in the GLB was limited by the slow biomass growth rate at 9 degrees C and low biomass retention in the reactor. Nevertheless, this study demonstrated the potential sulfate reduction rate of psychrotolerant sulfate-reducing mesophiles at sub-optimal temperature.

Formation and use of biogenic jarosite carrier for high-rate iron oxidising biofilms.

Jarosite precipitates formed in iron oxidising bioreactors have been shown to harbour iron-oxidisers. The aim of this study was to develop an iron oxidising bioprocess where microorganisms are retained solely on biogenic jarosite particles. Based on preliminary experiments using a fluidised-bed bioreactor (FBR), the formed jarosite particles started to disintegrate and wash out at upflow velocities of ≥0.21 cm/s. Therefore, the generation and use of biogenic jarosite carrier was studied in an expanded-bed bioreactor (J-EBR) with an upflow velocity of 0.19 cm/s. Inside J-EBR, the jarosite particles formed granules of 0.5-3 mm containing 200-460 mg/g of attached biomass. The performance of J-EBR was compared with an activated carbon biofilm FBR at 0.82 cm/s upflow velocity (AC-FBR). At 35 ± 2 °C with a feed ferrous iron concentration of 10 g/l, the highest obtained iron oxidation rate of J-EBR (6.8 g/l/h) was 33% lower than that of AC-FBR (10.1 g/l/h). This was likely due to the 80% lower recirculation rate and subsequently higher oxygen mass transfer limitation in J-EBR compared to AC-FBR. The present study demonstrates that biogenic jarosite can be used for retainment of iron oxidising biofilms in expanded-bed bioreactors that oxidise iron at high rates.

The effect of start-up on energy recovery and compositional changes in brewery wastewater in bioelectrochemical systems.

Start-up of bioelectrochemical systems (BESs) fed with brewery wastewater was compared at different adjusted anode potentials (-200 and 0 mV vs. Ag/AgCl) and external resistances (50 and 1000 Ω). Current generation stabilized faster with the external resistances (9 ± 3 and 1.70 ± 0.04 A/m3 with 50 and 1000 Ω, respectively), whilst significantly higher current densities of 76 ± 39 and 44 ± 9 A/m3 were obtained with the adjusted anode potentials of -200 and 0 mV vs. Ag/AgCl, respectively. After start-up, when operated using 47 Ω external resistance, the current densities and Coulombic efficiencies of all BESs stabilized to 9.5 ± 2.9 A/m3 and 12 ± 2%, respectively, demonstrating that the start-up protocols were not critical for long-term BES operation in microbial fuel cell mode. With adjusted anode potentials, two times more biofilm biomass (measured as protein) was formed by the end of the experiment as compared to start-up with the fixed external resistances. After start-up, the organics in the brewery wastewater, mainly sugars and alcohols, were transformed to acetate (1360 ± 250 mg/L) and propionate (610 ± 190 mg/L). Optimized start-up is required for prompt BES recovery, for example, after process disturbances. Based on the results of this study, adjustment of anode potential to -200 mV vs. Ag/AgCl is recommended for fast BES start-up.

Low-temperature (9 degrees C) AMD treatment in a sulfidogenic bioreactor dominated by a mesophilic Desulfomicrobium species.

The possibilities for the treatment of low-temperature mine waste waters have not been widely studied. The amenability of low-temperature sulfate reduction for mine waste water treatment at 9 degrees C was studied in a bench-scale fluidized-bed bioreactor (FBR). Formate was used as the electron and carbon source. The first influent for the FBR was acidic, synthetic waste water containing iron, nutrients, and sulfate, followed by diluted barren bioleaching solution (DBBS). The average sulfate reduction rates were 8 mmol L(-1) day(-1) and 6 mmol L(-1) day(-1) with synthetic waste water and DBBS, respectively. The corresponding specific activities were 2.4 and 1.6 mmol SO(4)(2-) g VSS(-1) day(-1), respectively. The composition of the microbial community and the active species of the FBR was analyzed by extracting the DNA and RNA, followed by PCR-DGGE with the universal bacterial 16S rRNA gene primers and dsrB-primers specific for sulfate-reducing bacteria. The FBR microbial community was simple and stable and the dominant and active species belonged to the genus Desulfomicrobium. In summary, long-term operation of a low-temperature bioreactor resulted in enrichment of formate-utilizing, psychrotolerant mesophilic sulfate reducing bacteria.

Thermovorax subterraneus, gen. nov., sp. nov., a thermophilic hydrogen-producing bacterium isolated from geothermally active underground mine.

A thermophilic, rod-shaped, motile, Gram-positive, spore-forming bacterium strain 70B(T) was isolated from a geothermally active underground mine in Japan. The temperature and pH range for growth was 50-81 degrees C (optimum 71 degrees C) and 6.2-9.8 (optimum pH 7-7.5), respectively. Growth occurred in the presence 0-2% NaCl (optimum 1% NaCl). Strain 70B(T) could utilize glucose, fructose, mannose, mannitol, pyruvate, cellobiose and tryptone as substrates. Thiosulfate was used as electron acceptor. Major whole-cell fatty acids were iso-C(15:0), C(16:0) DMA (dimethyl acetal), C(16:0) and anteiso-C(15:0). The G+C mol% of the DNA was 44.2%. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that the closest relatives of strain 70B(T) were Thermosediminibacter oceani DSM 16646(T) (94% similarity) and Thermosediminibacter litoriperuensis DSM 16647 (93% similarity). The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain 70B(T) represents a novel species in a new genus, for which the name Thermovorax subterraneus gen. nov., sp. nov. is proposed. The type strain of Thermovorax subterraneus is 70B(T) (=DSM 21563 = JCM 15541).

Extracellular enzyme activities and nutrient availability during artificial groundwater recharge.

Natural organic matter (NOM) removal is the main objective of artificial groundwater recharge (AGR) for drinking water production and biodegradation plays a substantial role in this process. This study focused on the biodegradation of NOM and nutrient availability for microorganisms in AGR by the determination of extracellular enzyme activities (EEAs) and nutrient concentrations along a flow path in an AGR aquifer (Tuusula Water Works, Finland). Natural groundwater in the same area but outside the influence of recharge was used as a reference. Determination of the specific alpha-d-glucosidase (alpha-Glu), beta-d-glucosidase (beta-Glu), phosphomonoesterase (PME), leucine aminopeptidase (LAP) and acetate esterase (AEST) activities by fluorogenic model substrates revealed major increases in the enzymatic hydrolysis rates in the aquifer within a 10m distance from the basin. The changes in the EEAs along the flow path occurred simultaneously with decreases in nutrient concentrations. The results support the assumption that the synthesis of extracellular enzymes in aquatic environments is up and down regulated by nutrient availability. The EEAs in the basin sediment and pore water samples (down to 10cm) were in the same order of magnitude as in the basin water, suggesting similar nutritional conditions. Phosphorus was likely to be the limiting nutrient at this particular AGR site. Furthermore, the extracellular enzymes functioned in a synergistic and cooperative way.

Biodegradation of natural organic matter in long-term, continuous-flow experiments simulating artificial ground water recharge for drinking water production.

The role of biodegradation in the attenuation of natural organic matter (NOM) was investigated in long-term experiments that simulate artificial ground water recharge (AGR) for drinking water production. Lake water containing 5.8 mg L(-1) total organic carbon (TOC) was continuously fed into an 18.5-m-long sand column. During the 941 d of operation, on average 76 and 81% of TOC was removed within the first 0.6 m and the entire column length, respectively. Large molecular size fractions (approximately 1800-2200 Da) of NOM were removed more efficiently than smaller ones (approximately 250-1400 Da). The biodegradation of dissolved organic carbon (DOC) within the first 0.6 m, measured by the stable inorganic carbon isotope (delta13C) method, depended on temperature and hydraulic load: The extent of mineralization was 32% at 6 degrees C (Day 442) and 38% at 23 degrees C (Day 708) with a 0.3 m3 (m2d)(-1) hydraulic load and 52% at 5.5 degrees C (Day 883) with a 3.1 m3 (m2d) (-1) hydraulic load. The rest of the DOC removal was likely due to entrapment or sorption onto the sand particles. Decreases in DOC and the total cell counts in the water along the column were positively correlated (r = 0.99; P = 0.001). The accumulation of biomass was minor, with the highest concentration amounting to 7.2 mg g(-1) dw of sand. In summary, this study demonstrated that biodegradation has a key role in NOM removal in AGR and is dependent on temperature.

Fluidized bed bioreactor for multiple environmental engineering solutions.

Fluidized bed bioreactors (FBR) are characterized by two-phase mixture of fluid and solid, in which the bed of solid particles is fluidized by means of downward or upward recirculation stream. FBRs are widely used for multiple environmental engineering solutions, such as wastewater treatment, as well as some industrial applications. FBR offers many benefits such as compact bioreactor size due to short hydraulic retention time, long biomass retention on the carrier, high conversion rates due to fully mixed conditions and consequently high mass transfer rates, no channelling of flow, dilution of influent concentrations due to recycle flow, suitability for enrichment of microbes with low Km values. The disadvantages of FBRs include bioreactor size limitations due to the height-to-diameter ratio, high-energy requirements due to high recycle ratios, and long start-up period for biofilm formation. This paper critically reviews some of the key studies on biomass enrichment via immobilisation of low growth yield microorganisms, high-rates via fully mixed conditions, technical developments in FBRs and ways of overcoming toxic effects via solution recycling. This technology has many potential new uses as well as hydrodynamic characteristics, which enable high-rate environmental engineering and industrial applications.

Storing of exoelectrogenic anolyte for efficient microbial fuel cell recovery.

Starting up a microbial fuel cell (MFC) requires often a long-term culture enrichment period, which is a challenge after process upsets. The purpose of this study was to develop low-cost storage for MFC enrichment culture to enable prompt process recovery after upsets. Anolyte of an operating xylose-fed MFC was stored at different temperatures and for different time periods. Storing the anolyte for 1 week or 1 month at +4°C did not significantly affect power production, but the lag time for power production was increased from 2 days to 3 or 5 days, respectively. One month storing at -20°C increased the lag time to 7 days. The average power density in these MFCs varied between 1.2 and 1.7 W/m3. The share of dead cells (measured by live/dead staining) increased with storing time. After 6-month storage, the power production was insignificant. However, xylose removal remained similar in all cultures (99-100%) while volatile fatty acids production varied. The results indicate that fermentative organisms tolerated the long storage better than the exoelectrogens. As storing at +4°C is less energy intensive compared to freezing, anolyte storage at +4°C for a maximum of 1 month is recommended as start-up seed for MFC after process failure to enable efficient process recovery.

Ethanol and hydrogen production by two thermophilic, anaerobic bacteria isolated from Icelandic geothermal areas.

Microbial fermentations are potential producers of sustainable energy carriers. In this study, ethanol and hydrogen production was studied by two thermophilic bacteria (strain AK15 and AK17) isolated from geothermal springs in Iceland. Strain AK15 was affiliated with Clostridium uzonii (98.8%), while AK17 was affiliated with Thermoanaerobacterium aciditolerans (99.2%) based on the 16S rRNA gene sequence analysis. Both strains fermented a wide variety of sugar residues typically found in lignocellulosic materials, and some polysaccharides. In the batch cultivations, strain AK17 produced ethanol from glucose and xylose fermentations of up to 1.6 mol-EtOH/mol-glucose (80% of the theoretical maximum) and 1.1 mol-EtOH/mol-xylose (66%), respectively. The hydrogen yields by AK17 were up to 1.2 mol-H2/ mol-glucose (30% of the theoretical maximum) and 1.0 mol-H2/mol-xylose (30%). The strain AK15 produced hydrogen as the main fermentation product from glucose (up to 1.9 mol-H2/mol-glucose [48%]) and xylose (1.1 mol-H2/mol-xylose [33%]). The strain AK17 tolerated exogenously added ethanol up to 4% (v/v). The ethanol and hydrogen production performance from glucose by a co-culture of the strains AK15 and AK17 was studied in a continuous-flow bioreactor at 60 degrees C. Stable and continuous ethanol and hydrogen co-production was achieved with ethanol yield of 1.35 mol-EtOH/mol-glucose, and with the hydrogen production rate of 6.1 mmol/h/L (H2 yield of 0.80 mol-H2/mol-glucose). PCR-DGGE analysis revealed that the AK17 became the dominant bacterium in the bioreactor. In conclusion, strain AK17 is a promising strain for the co-production of ethanol and hydrogen with a wide substrate utilization spectrum, relatively high ethanol tolerance, and ethanol yields among the highest reported for thermoanaerobes.

Silicate mineral dissolution during heap bioleaching.

Silicate minerals are present in association with metal sulfides in ores and their dissolution occurs when the sulfide minerals are bioleached in heaps for metal recovery. It has previously been suggested that silicate mineral dissolution can affect mineral bioleaching by acid consumption, release of trace elements, and increasing the viscosity of the leach solution. In this study, the effect of silicates present in three separate samples in conjunction with chalcopyrite and a complex multi-metal sulfide ore on heap bioleaching was evaluated in column bioreactors. Fe(2+) oxidation was inhibited in columns containing chalcopyrite samples A and C that leached 1.79 and 1.11 mM fluoride, respectively but not in sample B that contained 0.14 mM fluoride. Microbial Fe(2+) oxidation inhibition experiments containing elevated fluoride concentrations and measurements of fluoride release from the chalcopyrite ores supported that inhibition of Fe(2+) oxidation during column leaching of two of the chalcopyrite ores was due to fluoride toxicity. Column bioleaching of the complex sulfide ore was carried out at various temperatures (7-50 degrees C) and pH values (1.5-3.0). Column leaching at pH 1.5 and 2.0 resulted in increased acid consumption rates and silicate dissolution such that it became difficult to filter the leach solutions and for the leach liquor to percolate through the column. However, column temperature (at pH 2.5) only had a minor effect on the acid consumption and silicate dissolution rates. This study demonstrates the potential negative impact of silicate mineral dissolution on heap bioleaching by microbial inhibition and liquid flow.

High-efficiency hydrogen production by an anaerobic, thermophilic enrichment culture from an Icelandic hot spring.

Dark fermentative hydrogen production from glucose by a thermophilic culture (33HL), enriched from an Icelandic hot spring sediment sample, was studied in two continuous-flow, completely stirred tank reactors (CSTR1, CSTR2) and in one semi-continuous, anaerobic sequencing batch reactor (ASBR) at 58 degrees C. The 33HL produced H2 yield (HY) of up to 3.2 mol-H2/mol-glucose along with acetate in batch assay. In the CSTR1 with 33HL inoculum, H2 production was unstable. In the ASBR, maintained with 33HL, the H2 production enhanced after the addition of 6 mg/L of FeSO4 x H2O resulting in HY up to 2.51 mol-H2/mol-glucose (H2 production rate (HPR) of 7.85 mmol/h/L). The H2 production increase was associated with an increase in butyrate production. In the CSTR2, with ASBR inoculum and FeSO4 supplementation, stable, high-rate H2 production was obtained with HPR up to 45.8 mmol/h/L (1.1 L/h/L) and HY of 1.54 mol-H2/mol-glucose. The 33HL batch enrichment was dominated by bacterial strains closely affiliated with Thermobrachium celere (99.8-100%). T. celere affiliated strains, however, did not thrive in the three open system bioreactors. Instead, Thermoanaerobacterium aotearoense (98.5-99.6%) affiliated strains, producing H2 along with butyrate and acetate, dominated the reactor cultures. This culture had higher H2 production efficiency (HY and specific HPR) than reported for mesophilic mixed cultures. Further, the thermophilic culture readily formed granules in CSTR and ASBR systems. In summary, the thermophilic culture as characterized by high H2 production efficiency and ready granulation is considered very promising for H2 fermentation from carbohydrates.