Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 10 de 10
Filtrar
1.
J Neurochem ; 124(5): 590-601, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23016960

RESUMO

High levels of resistance to spinosad, a macrocyclic lactone insecticide, have been reported previously in western flower thrips, Frankliniella occidentalis, an economically important insect pest of vegetables, fruit and ornamental crops. We have cloned the nicotinic acetylcholine receptor (nAChR) α6 subunit from F. occidentalis (Foα6) and compared the nucleotide sequence of Foα6 from susceptible and spinosad-resistant insect populations (MLFOM and R1S respectively). A single nucleotide change has been identified in Foα6, resulting in the replacement of a glycine (G) residue in susceptible insects with a glutamic acid (E) in resistant insects. The resistance-associated mutation (G275E) is predicted to lie at the top of the third α-helical transmembrane domain of Foα6. Although there is no direct evidence identifying the location of the spinosad binding site, the analogous amino acid in the C. elegans glutamate-gated chloride channel lies in close proximity (4.4 Å) to the known binding site of ivermectin, another macrocyclic lactone pesticide. The functional consequences of the resistance-associated mutation have been examined in the human nAChR α7 subunit. Introduction of an analogous (A272E) mutation in α7 abolishes the modulatory effects of spinosad whilst having no significant effect upon activation by acetylcholine, consistent with spinosad having an allosteric mechanism of action.


Assuntos
Resistência a Inseticidas/genética , Inseticidas/farmacologia , Macrolídeos/farmacologia , Mutação Puntual , Receptores Nicotínicos/genética , Tisanópteros/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Membrana Celular , Combinação de Medicamentos , Humanos , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Técnicas de Patch-Clamp
2.
PLoS Genet ; 6(6): e1000999, 2010 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-20585623

RESUMO

The aphid Myzus persicae is a globally significant crop pest that has evolved high levels of resistance to almost all classes of insecticide. To date, the neonicotinoids, an economically important class of insecticides that target nicotinic acetylcholine receptors (nAChRs), have remained an effective control measure; however, recent reports of resistance in M. persicae represent a threat to the long-term efficacy of this chemical class. In this study, the mechanisms underlying resistance to the neonicotinoid insecticides were investigated using biological, biochemical, and genomic approaches. Bioassays on a resistant M. persicae clone (5191A) suggested that P450-mediated detoxification plays a primary role in resistance, although additional mechanism(s) may also contribute. Microarray analysis, using an array populated with probes corresponding to all known detoxification genes in M. persicae, revealed constitutive over-expression (22-fold) of a single P450 gene (CYP6CY3); and quantitative PCR showed that the over-expression is due, at least in part, to gene amplification. This is the first report of a P450 gene amplification event associated with insecticide resistance in an agriculturally important insect pest. The microarray analysis also showed over-expression of several gene sequences that encode cuticular proteins (2-16-fold), and artificial feeding assays and in vivo penetration assays using radiolabeled insecticide provided direct evidence of a role for reduced cuticular penetration in neonicotinoid resistance. Conversely, receptor radioligand binding studies and nucleotide sequencing of nAChR subunit genes suggest that target-site changes are unlikely to contribute to resistance to neonicotinoid insecticides in M. persicae.


Assuntos
Afídeos/genética , Sistema Enzimático do Citocromo P-450/genética , Amplificação de Genes/efeitos dos fármacos , Proteínas de Insetos/genética , Resistência a Inseticidas , Inseticidas/farmacologia , Nicotina/farmacologia , Animais , Afídeos/química , Afídeos/efeitos dos fármacos , Sequência de Bases , Sistema Enzimático do Citocromo P-450/química , Dosagem de Genes , Proteínas de Insetos/química , Dados de Sequência Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Alinhamento de Sequência
3.
BMC Neurosci ; 12: 51, 2011 May 31.
Artigo em Inglês | MEDLINE | ID: mdl-21627790

RESUMO

BACKGROUND: Myzus persicae is a globally important aphid pest with a history of developing resistance to insecticides. Unusually, neonicotinoids have remained highly effective as control agents despite nearly two decades of steadily increasing use. In this study, a clone of M. persicae collected from southern France was found, for the first time, to exhibit sufficiently strong resistance to result in loss of the field effectiveness of neonicotinoids. RESULTS: Bioassays, metabolism and gene expression studies implied the presence of two resistance mechanisms in the resistant clone, one based on enhanced detoxification by cytochrome P450 monooxygenases, and another unaffected by a synergist that inhibits detoxifying enzymes. Binding of radiolabeled imidacloprid (a neonicotinoid) to whole body membrane preparations showed that the high affinity [3H]-imidacloprid binding site present in susceptible M. persicae is lost in the resistant clone and the remaining lower affinity site is altered compared to susceptible clones. This confers a significant overall reduction in binding affinity to the neonicotinoid target: the nicotinic acetylcholine receptor (nAChR). Comparison of the nucleotide sequence of six nAChR subunit (Mpα1-5 and Mpß1) genes from resistant and susceptible aphid clones revealed a single point mutation in the loop D region of the nAChR ß1 subunit of the resistant clone, causing an arginine to threonine substitution (R81T). CONCLUSION: Previous studies have shown that the amino acid at this position within loop D is a key determinant of neonicotinoid binding to nAChRs and this amino acid change confers a vertebrate-like character to the insect nAChR receptor and results in reduced sensitivity to neonicotinoids. The discovery of the mutation at this position and its association with the reduced affinity of the nAChR for imidacloprid is the first example of field-evolved target-site resistance to neonicotinoid insecticides and also provides further validation of exisiting models of neonicotinoid binding and selectivity for insect nAChRs.


Assuntos
Afídeos/genética , Colinérgicos/farmacologia , Imidazóis/farmacologia , Inseticidas/farmacologia , Nitrocompostos/farmacologia , Receptores Nicotínicos/genética , Animais , Afídeos/metabolismo , Resistência a Inseticidas/genética , Mutação , Neonicotinoides , Receptores Nicotínicos/metabolismo
4.
PLoS One ; 16(7): e0254251, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34234379

RESUMO

Pentameric ligand-gated ion channels (pLGICs) activated by the inhibitory neurotransmitter γ-aminobutyric acid (GABA) are expressed widely in both vertebrate and invertebrate species. One of the best characterised insect GABA-gated chloride channels is RDL, an abbreviation of 'resistance to dieldrin', that was originally identified by genetic screening in Drosophila melanogaster. Here we have cloned the analogous gene from the bumblebee Bombus terrestris audax (BtRDL) and examined its pharmacological properties by functional expression in Xenopus oocytes. Somewhat unexpectedly, the sensitivity of BtRDL to GABA, as measured by its apparent affinity (EC50), was influenced by heterologous expression conditions. This phenomenon was observed in response to alterations in the amount of cRNA injected; the length of time that oocytes were incubated before functional analysis; and by the presence or absence of a 3' untranslated region. In contrast, similar changes in expression conditions were not associated with changes in apparent affinity with RDL cloned from D. melanogaster (DmRDL). Changes in apparent affinity with BtRDL were also observed following co-expression of a chaperone protein (NACHO). Similar changes in apparent affinity were observed with an allosteric agonist (propofol) and a non-competitive antagonist (picrotoxinin), indicating that expression-depended changes are not restricted to the orthosteric agonist binding site. Interestingly, instances of expression-dependent changes in apparent affinity have been reported previously for vertebrate glycine receptors, which are also members of the pLGIC super-family. Our observations with BtRDL are consistent with previous data obtained with vertebrate glycine receptors and indicates that agonist and antagonist apparent affinity can be influenced by the level of functional expression in a variety of pLGICs.


Assuntos
Canais de Cloreto/antagonistas & inibidores , Canais de Cloreto/metabolismo , Drosophila melanogaster/metabolismo , Ácido gama-Aminobutírico/metabolismo , Regiões 3' não Traduzidas/efeitos dos fármacos , Sequência de Aminoácidos , Animais , Abelhas/metabolismo , Agonistas dos Canais de Cloreto/farmacologia , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/efeitos dos fármacos , Feminino , Picrotoxina/análogos & derivados , Picrotoxina/farmacologia , Propofol/farmacologia , Receptores de Glicina/metabolismo , Sesterterpenos , Xenopus laevis/metabolismo
5.
Insect Biochem Mol Biol ; 104: 58-64, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30550974

RESUMO

Glutamate-gated chloride channels (GluCls) are found only in invertebrates and mediate fast inhibitory neurotransmission. The structural and functional diversity of GluCls are produced through assembly of multiple subunits and via posttranscriptional alternations. Alternative splicing is the most common way to achieve this in insect GluCls and splicing occurs primarily at exons 3 and 9. As expression pattern and pharmacological properties of exon 9 alternative splices in invertebrate GluCls remain poorly understood, the cDNAs encoding three alternative splice variants (9a, 9b and 9c) of the PxGluCl gene from the diamondback moth Plutella xylostella were constructed and their pharmacological characterizations were examined using electrophysiological studies. Alternative splicing of exon 9 had little to no impact on PxGluCl sensitivity towards the agonist glutamate when subunits were singly or co-expressed in Xenopus oocytes. In contrast, the allosteric modulator abamectin and the chloride channel blocker fipronil had differing effects on PxGluCl splice variants. PxGluCl9c channels were more resistant to abamectin and PxGluCl9b channels were more sensitive to fipronil than other homomeric channels. In addition, heteromeric channels containing different splice variants showed similar sensitivity to abamectin (except for 9c) and reduced sensitivity to fipronil than homomeric channels. These findings suggest that functionally indistinguishable but pharmacologically distinct GluCls could be formed in P. xylostella and that the upregulated constitutive expression of the specific variants may contribute to the evolution of insecticide resistance in P. xylostella and other arthropods.


Assuntos
Processamento Alternativo/efeitos dos fármacos , Canais de Cloreto , Éxons , Proteínas de Insetos , Resistência a Inseticidas , Ivermectina/análogos & derivados , Pirazóis/farmacologia , Animais , Canais de Cloreto/antagonistas & inibidores , Canais de Cloreto/biossíntese , Canais de Cloreto/genética , Proteínas de Insetos/antagonistas & inibidores , Proteínas de Insetos/biossíntese , Proteínas de Insetos/genética , Resistência a Inseticidas/efeitos dos fármacos , Resistência a Inseticidas/genética , Ivermectina/farmacologia , Mariposas/genética , Mariposas/metabolismo
6.
Insect Biochem Mol Biol ; 86: 50-57, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28576654

RESUMO

Abamectin is one of the most widely used avermectins for agricultural pests control, but the emergence of resistance around the world is proving a major threat to its sustained application. Abamectin acts by directly activating glutamate-gated chloride channels (GluCls) and modulating other Cys-loop ion channels. To date, three mutations occurring in the transmembrane domain of arthropod GluCls are associated with target-site resistance to abamectin: A309V in Plutella xylostella GluCl (PxGluCl), G323D in Tetranychus urticae GluCl1 (TuGluCl1) and G326E in TuGluCl3. To compare the effects of these mutations in a single system, A309V/I/G and G315E (corresponding to G323 in TuGluCl1 and G326 in TuGluCl3) substitutions were introduced individually into the PxGluCl channel. Functional analysis using Xenopus oocytes showed that the A309V and G315E mutations reduced the sensitivity to abamectin by 4.8- and 493-fold, respectively. In contrast, the substitutions A309I/G show no significant effects on the response to abamectin. Interestingly, the A309I substitution increased the channel sensitivity to glutamate by one order of magnitude (∼12-fold). Analysis of PxGluCl homology models indicates that the G315E mutation interferes with abamectin binding through a steric hindrance mechanism. In contrast, the structural consequences of the A309 mutations are not so clear and an allosteric modification of the binding site is the most likely mechanism. Overall the results show that both A309V and G315E mutations may contribute to target-site resistance to abamectin and may be important for the future prediction and monitoring of abamectin resistance in P. xylostella and other arthropod pests.


Assuntos
Canais de Cloreto/genética , Inseticidas , Ivermectina/análogos & derivados , Mariposas/genética , Sequência de Aminoácidos , Animais , Canais de Cloreto/metabolismo , Ácido Glutâmico/metabolismo , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Resistência a Inseticidas/genética , Dados de Sequência Molecular , Mariposas/metabolismo , Mutação , Xenopus laevis
7.
FEBS Lett ; 589(5): 598-607, 2015 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-25637326

RESUMO

We describe the identification in aphids of a unique heterodimeric voltage-gated sodium channel which has an atypical ion selectivity filter and, unusually for insect channels, is highly insensitive to tetrodotoxin. We demonstrate that this channel has most likely arisen by adaptation (gene fission or duplication) of an invertebrate ancestral mono(hetero)meric channel. This is the only identifiable voltage-gated sodium channel homologue in the aphid genome(s), and the channel's novel selectivity filter motif (DENS instead of the usual DEKA found in other eukaryotes) may result in a loss of sodium selectivity, as indicated experimentally in mutagenised Drosophila channels.


Assuntos
Afídeos/metabolismo , Canais de Sódio Disparados por Voltagem/química , Canais de Sódio Disparados por Voltagem/metabolismo , Animais , Evolução Molecular , Tetrodotoxina/metabolismo
8.
Insect Biochem Mol Biol ; 51: 41-51, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24855024

RESUMO

The peach potato aphid, Myzus persicae is a globally distributed crop pest with a host range of over 400 species including many economically important crop plants. The intensive use of insecticides to control this species over many years has led to populations that are now resistant to several classes of insecticide. Work spanning over 40 years has shown that M. persicae has a remarkable ability to evolve mechanisms that avoid or overcome the toxic effect of insecticides with at least seven independent mechanisms of resistance described in this species to date. The array of novel resistance mechanisms, including several 'first examples', that have evolved in this species represents an important case study for the evolution of insecticide resistance and also rapid adaptive change in insects more generally. In this review we summarise the biochemical and molecular mechanisms underlying resistance in M. persicae and the insights study of this topic has provided on how resistance evolves, the selectivity of insecticides, and the link between resistance and host plant adaptation.


Assuntos
Afídeos/efeitos dos fármacos , Afídeos/genética , Evolução Biológica , Resistência a Inseticidas/genética , Inseticidas/farmacologia , Animais
9.
Pest Manag Sci ; 69(2): 195-9, 2013 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22528746

RESUMO

BACKGROUND: Myzus persicae is a globally important aphid pest that is mainly controlled through the application of chemical insecticides. Recently, a clone of M. persicae exhibiting control-compromising levels of resistance to neonicotinoid insecticides was described. The resistance of this clone was associated with reduced affinity of imidacloprid for the target site (the nicotinic acetylcholine receptor) as a result of mutation of a key amino acid residue (R81T) in the loop D region of a nAChR ß1 subunit. The potent levels of resistance conferred by this mechanism are cause for considerable concern, and the frequency and distribution of the mutation in worldwide populations of M. persicae require careful monitoring. In this study, a high-throughput assay has been developed that allows detection of the mutation in individual aphids. RESULTS: A real-time TaqMan assay to detect the R81T substitution was developed that proved to be sensitive and specific in tests of analytical sensitivity and in a blind genotyping trial of DNA extracted from individual aphids comprising the three possible genotypes. The assay was then used to examine the frequency of the R81T mutation in aphids collected and stored in ethanol from peach orchards in southern France. The R81T frequency varied from 33 to 100% in seven populations from the department of Gard, France. CONCLUSIONS: This study describes a rapid and sensitive assay that very effectively detects the R81T mutation in individual aphids. The results also have practical significance for the control of M. persicae in southern France and provide contemporary data to inform current resistance management strategies.


Assuntos
Afídeos/genética , Imidazóis/farmacologia , Proteínas de Insetos/genética , Resistência a Inseticidas , Inseticidas/farmacologia , Mutação de Sentido Incorreto , Nitrocompostos/farmacologia , Receptores Nicotínicos/genética , Animais , Afídeos/efeitos dos fármacos , Afídeos/metabolismo , Proteínas de Insetos/metabolismo , Neonicotinoides , Antagonistas Nicotínicos/farmacologia , Reação em Cadeia da Polimerase em Tempo Real , Receptores Nicotínicos/metabolismo
10.
Environ Pollut ; 158(9): 2977-84, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20615598

RESUMO

Mytilus edulis were exposed to 17beta-estradiol (E2) and the synthetic estrogens ethinyl estradiol (EE2) and estradiol benzoate (EB) for 10 days. Two exposures were performed to determine their effect on vitellogenin (VTG) and estrogen receptor 2 (ER2) mRNA expression at different stages of the reproductive cycle. Significant natural variation was not observed in VTG mRNA expression, though ER2 mRNA expression displayed significantly lower values during January, February and July compared with other times of the year. A significant increase in VTG and ER2 mRNA expression was observed in mussels exposed to estrogens at the early stage of gametogenesis. In contrast, mature mussels displayed no statistically significant change in the VTG or ER2 mRNA expression. The data presented suggests that the reproductive physiology of molluscs, in terms of VTG and ER2 mRNA expression, may be susceptible to damage by environmental estrogens at certain points in their gametogenesis process.


Assuntos
Disruptores Endócrinos/toxicidade , Estrogênios/toxicidade , Gametogênese/efeitos dos fármacos , Mytilus edulis/efeitos dos fármacos , Poluentes Químicos da Água/toxicidade , Animais , Receptor beta de Estrogênio/genética , Receptor beta de Estrogênio/metabolismo , Feminino , Masculino , Mytilus edulis/genética , Mytilus edulis/metabolismo , RNA Mensageiro/metabolismo , Vitelogeninas/genética , Vitelogeninas/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA