RESUMO
Most plant viruses require insect vectors for transmission. One of the key steps for the transmission of persistent-circulative plant viruses is overcoming the gut barrier to enter epithelial cells. To date, little has been known about viral cofactors in gut epithelial cells of insect vectors. Here, we identified flotillin 2 as a plasma membrane protein that facilitates the infection of rice stripe virus (RSV) in its vector, the small brown planthopper. Flotillin 2 displayed a prominent plasma membrane location in midgut epithelial cells. The nucleocapsid protein of RSV and flotillin 2 colocalized on gut microvilli, and a nanomolar affinity existed between the two proteins. Knockout of flotillin 2 impeded the entry of virions into epithelial cells, resulting in a 57% reduction of RSV levels in planthoppers. The knockout of flotillin 2 decreased disease incidence in rice plants fed by viruliferous planthoppers from 40% to 11.7%. Furthermore, flotillin 2 mediated the infection of southern rice black-streaked dwarf virus in its vector, the white-backed planthopper. This work implies the potential of flotillin 2 as a target for controlling the transmission of rice stripe disease. IMPORTANCE Plant viral diseases are a major threat to world agriculture. The transmission of 80% of plant viruses requires vector insects, and 54% of vector-borne plant viruses are persistent-circulative viruses, which must overcome the barriers of gut cells with the help of proteins on the cell surface. Here, we identified flotillin 2 as a membrane protein that mediates the cell entry of rice stripe virus in its vector insect, small brown planthopper. Flotillin 2 displays a prominent cellular membrane location in midgut cells and can specifically bind to virions. The loss of flotillin 2 impedes the entry of virions into the midgut cells of vector insects and substantially suppresses viral transmission to rice. Therefore, flotillin 2 may be a promising target gene for manipulation in vector insects to control the transmission of rice stripe disease and perhaps that of other rice virus diseases in the future.
Assuntos
Proteínas de Insetos , Proteínas de Membrana , Oryza , Vírus de Plantas , Tenuivirus , Animais , Hemípteros/virologia , Proteínas de Insetos/genética , Proteínas de Insetos/metabolismo , Insetos Vetores/virologia , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Oryza/virologia , Doenças das Plantas/virologia , Vírus de Plantas/fisiologia , Tenuivirus/genética , Tenuivirus/metabolismoRESUMO
BACKGROUND: Pine wilt disease as a devastating forest disaster result from Bursaphelenchus xylophilus that spread by stem-borers Monochamus alternatus feeding on pine leaves, which has brought inestimable economic losses to the world's forestry due to lack of effective prevention and control measures. In this paper, we put forward a proposal for utilizing nanoHKUST-1 to encapusulate the Pyrethrins II that a nerve agent extracted from plant to control M. alternatus, including toxicity mechanism research, traceable biopesticide monitoring, and environment assessment for the first time. The highly biocompatible nanoHKUST-1 can solve the problems of poor water solubility, easy degradation and low control efficiency of Pyrethrins II. RESULTS: The results illustrated the biopesticide loading efficiency of PthII@HKUST-1 reached 85% and the cumulative release of pH-dependent PthII@HKUST-1 was up to 15 days (90%), and also effectively avoid photodegradation (pH 7.0, retention 60.9%). 50 nm PthII@HKUST-1 made it easily penetrate the body wall of MA larvae and transmit to tissue cells through contact and diffusion. Moreover, PthII@HKUST-1 can effectively enhance the cytotoxicity and utilization of Pyrethrins II, which will provide valuable research value for the application of typical plant-derived nerve agents in the prevention and control of forestry pests. PthII@HKUST-1 as an environmentally friendly nano-pesticide can efficiently deliver Pyrethrins II to the larval intestines and absorbed by the larvae. PthII@HKUST-1 could also be transmitted to the epidemic wood and dead wood at a low concentration (10 mg/L). CONCLUSION: Here we speculate that nanoHKUST-1 will bring new opportunity to research biopesticide inhibition mechanism of different agricultural and forestry pests, which will break through the existing research limitations on development, utilization and traceable monitoring of biopesticide, especially for the study of targeting specific proteins.
Assuntos
Besouros , Praguicidas , Pinus , Piretrinas , Animais , Agentes de Controle Biológico/farmacologia , Larva , Praguicidas/farmacologia , Piretrinas/farmacologiaRESUMO
Sulfonic acid-functionalized covalent organic frameworks (COF-SO3) as a coating of stir bar sorptive extraction (SBSE) for capturing three fluoroquinolones from milk have been developed. The COF-SO3 material was characterized by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, and infrared spectroscopy. Milk without any typical treatments like protein precipitation and defatting was only diluted five times with water for test. Combined with high-performance liquid chromatography (HPLC), a SBSE-HPLC method was established for detecting fluoroquinolones in milk samples. The corresponding wide linear ranges (4.00-500.0 µg L-1), low detection limits (1.20-2.62 µg L-1), good test repeatability (RSD < 5.2%), and acceptable enrichment factors (56.2-61.5) were implemented for three fluoroquinolones. The analytical method was applied to determine trace targets and provided satisfactory results. Furthermore, the research displayed satisfied reproducibility for bar-to-bar (RSD < 6.5%) and batch-to-batch (RSD < 8.6%) tests.
Assuntos
Fluoroquinolonas , Estruturas Metalorgânicas , Animais , Fluoroquinolonas/análise , Leite/química , Ácidos Sulfônicos , Reprodutibilidade dos TestesRESUMO
Microporous organic networks (MONs) are highly porous materials that are particularly useful in analytical chemistry. However, the use of these materials is often limited by the functional groups available on their surface. Here, we described the polymerization of a sea urchin-like structure material at ambient temperature, that was functionalized with hydroxyl, carboxyl, and triazine groups and denoted as OH-COOH-MON-TEPT. A substantial proportion of OH-COOH-MON-TEPT was intricately decorated EDA-Fe3O4, creating a well-designed configuration (EDA-Fe3O4 @OH-COOH-MON-TEPT-EDC) for superior adsorption of the target analytes phenylurea herbicides (PUHs) via magnetic solid-phase extraction (MSPE). The proposed method showed remarkably low limits of detection ranging from 0.03 to 0.22 ng·L-1. Experimental investigations and theoretical analyses unveiled the adsorption mode between EDA-Fe3O4 @OH-COOH-MON-TEPT-EDC and PUHs. These findings establish a robust foundation for potential applications of EDA-Fe3O4 @OH-COOH-MON-TEPT-EDC in the analysis of various polar contaminants.
RESUMO
Pine wilt disease is a devastating forest disaster caused by Bursaphelenchus xylophilus, which has brought inestimable economic losses to the world's forestry due to lack of effective prevention and control measures. In this paper, a porous structure CuBTC was designed to deliver avermectin (AM) and a control vector insect Japanese pine sawyer (JPS) of B. xylophilus, which can improve the biocompatibility, anti-photolysis and delivery efficacy of AM. The results illustrated the cumulative release of pH-dependent AM@CuBTC was up to 12 days (91.9%), and also effectively avoided photodegradation (pH 9.0, 120 h, retention 69.4%). From the traceable monitoring experiment, the AM@CuBTC easily penetrated the body wall of the JPS larvae and was transmitted to tissue cells though contact and diffusion. Furthermore, AM@CuBTC can effectively enhance the cytotoxicity and utilization of AM, which provides valuable research value for the application of typical plant-derived nerve agents in the prevention and control of forestry pests. AM@CuBTC as an environmentally friendly nanopesticide can efficiently deliver AM to the larval intestines where it is absorbed by the larvae. AM@CuBTC can be transmitted to the epidemic wood and dead wood at a low concentration (10 mg/L).
RESUMO
Contamination of perfluoroalkyl substances (PFASs) in agricultural products have attracted more and more attentions recently. In this review, relationship between PFASs and vegetables is summarized comprehensively. PFASs could transfer to cultivation soils by irrigation water, bio-amended soil, and atmospheric deposition mainly from industrial emissions. Carbon chain length of PFASs, species of vegetables and so on are key factors for PFASs migration and bioaccumulation in soils, plants and vegetables. Studies on food risk assessment of PFOA and PFOS show low consumption risk for most vegetables, however researches on other substances are lacking. In the future, we need to pay more attention on novel pollution pathway in cultivation, traceability research for considerable contamination, dietary exposure levels for different vegetables and more substances, as well as more exact and scientific food risk assessments. Additionally, effective means for PFASs adsorption in soil and removal from soil are also expected.
Assuntos
Bioacumulação , Fluorocarbonos/metabolismo , Contaminação de Alimentos/análise , Verduras/metabolismo , Alquilação , Fluorocarbonos/química , Medição de RiscoRESUMO
Novel core shell structured magnetic covalent organic frameworks were synthesized at room temperature and first applied in water samples for the enrichment of trace polybrominated diphenyl ethers (PBDEs) through magnetic solid-phase extraction. The prepared materials were characterized through transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, vibrating sample magnetometer and X-ray photoelectron spectroscopy. During adsorption, the parameters affecting extraction and desorption efficiency were further optimized. Combined gas chromatography and mass spectrometry (GC/MS) revealed that high enrichment factors (275-292), low limits of detection (0.12-0.38 ng·L-1), wide linear ranges (0.5-1000 ng·L-1), and good reproducibility (intra-day 1.40%-4.31% and inter-day 5.14%-9.12%) were obtained under optimal conditions. The method successfully detected PBDEs in different water samples.
RESUMO
Angiotensin-converting enzyme (ACE) plays diverse roles in the animal kingdom. However, whether ACE plays an immune function against viral infection in vector insects is unclear. In this study, an ACE gene (LsACE) from the small brown planthopper was found to respond to Rice stripe virus (RSV) infection. The enzymatic activities of LsACE were characterized at different pH and temperature. Twenty planthopper proteins were found to interact with LsACE. RSV infection significantly upregulated LsACE expression in the testicle and fat body. When the expression of LsACE in viruliferous planthoppers was inhibited, the RNA level of the RSV SP gene was upregulated 2-fold in planthoppers, and all RSV genes showed higher RNA levels in the rice plants consumed by these planthoppers, leading to a higher viral infection rate and disease rating index. These results indicate that LsACE plays a role in the immune response against RSV transmission by planthoppers.
Assuntos
Hemípteros/imunologia , Hemípteros/virologia , Proteínas de Insetos/imunologia , Insetos Vetores/imunologia , Insetos Vetores/virologia , Peptidil Dipeptidase A/imunologia , Tenuivirus/fisiologia , Sequência de Aminoácidos , Animais , Hemípteros/genética , Hemípteros/fisiologia , Proteínas de Insetos/genética , Insetos Vetores/genética , Insetos Vetores/fisiologia , Oryza/virologia , Peptidil Dipeptidase A/genética , Filogenia , Doenças das Plantas/virologia , Tenuivirus/classificação , Tenuivirus/genética , Tenuivirus/isolamento & purificaçãoRESUMO
The vertical and horizontal distributions of Pseudorobitis gibbus adults on Lagerstroemia indica were analyzed by using the aggregation indices and geostatistics. The results from aggregation index methods indicated that its vertical distribution was an aggregated distribution, and the aggregation average (lambda) was above two, showing that the aggregation resulted from its bionomics and behaviors, not from the environmental factors. The results of geostatistic analysis showed that the best fitting models for the five different sampling data in June 4, June 16, June 29, July 25 and August 22 were Gaussian, Gaussian, Linear, Gaussian and Linear models, respectively, but random models in July 12 and August 7. It was concluded that the horizontal distribution of the pest was aggregated on the whole, and there existed a spatial correlation ranging from 1.68 to 9.79 m on the different dates.
Assuntos
Besouros , Animais , China , Lagerstroemia , Modelos Lineares , Distribuição Normal , Densidade Demográfica , Dinâmica Populacional , Análise EspacialRESUMO
Based on a modified quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation method with Fe3O4 magnetic nanoparticles (MNPs) as the adsorbing material and gas chromatography-tandem mass spectrometry (GC-MS/MS) determination in multiple reaction monitoring (MRM) mode, we established a new method for the determination of multiple pesticides in vegetables and fruits. It was determined that bare MNPs have excellent function as adsorbent when purified, and it is better to be separated from the extract. The amount of MNPs influenced the clean-up performance and recoveries. To achieve the optimum performance of modified QuEChERS towards the target analytes, several parameters including the amount of the adsorbents and purification time were investigated. Under the optimum conditions, recoveries were evaluated in four representative matrices (tomato, cucumber, orange and apple) with the spiked concentrations of 10 µg kg(-1), 50 µg kg(-1)and 200 µg kg(-1) in all cases. The results showed that the recovery of 101 pesticides ranged between 71.5 and 111.7%, and the relative standard deviation was less than 10.5%. The optimum clean-up system improved the purification efficiency and simultaneously obtained satisfactory recoveries of multiple pesticides, including planar-ring pesticides. In short, the modified QuEChERS method in addition to MNPs used for removing impurities improved the speed of sample pre-treatment and exhibited an enhanced performance and purifying effect.
Assuntos
Compostos Férricos/química , Frutas/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Nanopartículas de Magnetita/química , Resíduos de Praguicidas/análise , Espectrometria de Massas em Tandem/métodos , Verduras/genética , Cromatografia Gasosa-Espectrometria de Massas/economia , Nanopartículas de Magnetita/ultraestrutura , Microscopia Eletrônica de Transmissão , Espectrometria de Massas em Tandem/economia , Fatores de Tempo , Verduras/químicaRESUMO
A method for analyzing volatile constituents from plant leaves with gas chromatography/mass spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) was developed. The volatile compounds from the plant leaves inside a sealed flask maintained at 45 degrees C in a water bath were efficiently extracted with Polyacrylate (85 microm) SPME fibers prior to perform GC/MS analysis. The GC/MS analysis indicated that the volatile compounds from the plant leaves which is easy to be damaged by Tetraychus vienneis include relative large amounts of cis-3-hexenyl ester acetic acid, cis-3-hexenyl ester butanoic acid and alpha-famesene. These compounds were preliminarily confirmed to be accountable for attracting Tetraychus vienneis. This finding may lead to identify biological species for preventing and treating Tetraychus vienneis.