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OBJECTIVE: To evaluate the artifact expression of a new material-polylactic acid (PLA)/hydroxyapatite (HA)/graphene oxide (GO) nanocomposite-and compare it with that of commonly used dental materials, using cone-beam computed tomography. MATERIALS AND METHODS: Cylinders of amalgam alloy, metal alloy, titanium, gutta-percha, and PLA/HA/GO were individually placed in the center of an acrylic phantom. Three images of each phantom + cylinder set were acquired using a Picasso Trio unit (Vatech, Hwaseong, South Korea) set at 90 kVp, 3.7 mA, 0.2 mm3 voxel size, and 12 × 8.5 cm FOV. Three images of a control group (sound phantom) were also obtained. Eight ROIs were established in each image to evaluate the standard deviation (S.D.) of gray values in the ImageJ Software. The Kruskal-Wallis test with the Student-Newman-Keuls post hoc was employed, considering a significance level of 5%. RESULTS: There were no significant differences in S.D. between the control and PLA/HA/GO images (p = 0.712). Both control and PLA/HA/GO showed fewer image artifacts than the other materials (p < 0.05). The images of the amalgam alloy did not differ from the metal alloy images (p = 0.691), showing more artifacts than the gutta-percha (p = 0.028) and titanium (p = 0.051). CONCLUSIONS: The PLA/HA/GO produced fewer artifacts and a better-quality image than the other tested materials. CLINICAL RELEVANCE: The PLA/HA/GO has physical and biological properties similar to those of dental materials. Since it has produced fewer image artifacts, this new nanocomposite may be employed in the near future, enhancing the diagnosis in CBCT.
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Materiais Dentários/química , Durapatita/química , Grafite/química , Nanocompostos/química , Poliésteres/química , Artefatos , Tomografia Computadorizada de Feixe Cônico , Imagens de FantasmasRESUMO
OBJECTIVE: The aim of this study is to evaluate the efficacy of metal artefact reduction (MAR) in different dental materials with Picasso Trio cone-beam computed tomography (CBCT) scanner. MATERIALS AND METHODS: Three imaging phantoms were custom-made of acrylic resin. Each phantom presented three cylinders of the same material: dental amalgam alloy, gutta-percha or aluminium-copper alloy. CBCT scans were performed on Picasso Trio unit with and without MAR, and artefact expression (standard deviation of grey values) was obtained and compared by Kruskal-Wallis and Student-Newman-Keuls (post hoc) (α = 0.05). RESULTS: Significant reduction of artefact expression (p < 0.05) was observed with MAR on areas around dental alloys. No significant difference (p > 0.05) was observed with or without MAR when gutta-percha was scanned. CONCLUSION: MAR was effective in reducing artefacts arising from dental alloys on CBCT images. CLINICAL RELEVANCE: Dental materials of high atomic number and density are widely used in dentistry and can produce artefact that compromise CBCT image. The present study demonstrated that metal artefact reduction algorithm is an effective tool to improve image quality.
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Artefatos , Tomografia Computadorizada de Feixe Cônico/métodos , Ligas Dentárias , Amálgama Dentário , Materiais Dentários , Guta-Percha , Alumínio , Cobre , Imagens de FantasmasRESUMO
Objectives: The aim of this study was to evaluate the influence of peak kilovoltage (kVp) and a metal artifact reduction (MAR) tool on image quality and the diagnosis of vertical root fracture (VRF) in cone-beam computed tomography (CBCT). Materials and Methods: Twenty single-rooted human teeth filled with an intracanal metal post were divided into 2 groups: control (n = 10) and VRF (n = 10). Each tooth was placed into the socket of a dry mandible, and CBCT scans were acquired using a Picasso Trio varying the kVp (70, 80, 90, or 99), and the use of MAR (with or without). The examinations were assessed by 5 examiners for the diagnosis of VRF using a 5-point scale. A subjective evaluation of the expression of artifacts was done by comparing random axial images of the studied protocols. The results of the diagnoses were analyzed using 2-way analysis of variance and the Tukey post hoc test, the subjective evaluations were compared using the Friedman test, and intra-examiner reproducibility was evaluated using the weighted kappa test (α = 5%). Results: The kVp and MAR did not influence the diagnosis of VRF (p > 0.05). According to the subjective classification, the 99 kVp protocol with MAR demonstrated the least expression of artifacts, while the 70 kVp protocol without MAR led to the most artifacts. Conclusions: Protocols with higher kVp combined with MAR improved the image quality of CBCT examinations. However, those factors did not lead to an improvement in the diagnosis of VRF.
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Purpose: The aim of this study was to evaluate changes in the trabecular bone through texture analysis and compare the texture analysis characteristics of different areas in patients with medication-related osteonecrosis of the jaw (MRONJ). Materials and Methods: Cone-beam computed tomographic images of 16 patients diagnosed with MRONJ were used. In sagittal images, 3 regions were chosen: active osteonecrosis (AO); intermediate tissue (IT), which presented a zone of apparently healthy tissue adjacent to the AO area; and healthy bone tissue (HT) (control area). Texture analysis was performed evaluating 7 parameters: secondary angular momentum, contrast, correlation, sum of squares, inverse moment of difference, sum of entropies, and entropy. Data were analyzed using the Kruskal-Wallis test with a significance level of 5%. Results: Comparing the areas of AO, IT, and HT, significant differences (P<0.05) were observed. The IT and AO area images showed higher values for parameters such as contrast, entropy, and secondary angular momentum than the HT area, indicating greater disorder in these tissues. Conclusion: Through texture analysis, changes in the bone pattern could be observed in areas of osteonecrosis. The texture analysis demonstrated that areas visually identified and classified as IT still had necrotic tissue, thereby increasing the accuracy of delimiting the real extension of MRONJ.
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OBJECTIVE: This study was developed to evaluate the influence of voxel size on bone measurements for implant planning. MATERIALS AND METHODS: The research was performed by using edentulous synthetic human mandibles with different levels of bone resorption. For each mandible, height and bone thickness were measured with a digital caliper. The PaX-i3d device was used to acquire the volumes of the five mandibles, with 50kVp, 4 mA, and a voxel size of 0.08 mm. After the acquisition, the images were reconstructed in the software CS three-dimensional Imaging, with four different sizes of voxels: 0.1, 0.2, 0.3, and 0.4 mm. All volumes were analyzed by a single evaluator who performed measurements to obtain bone height and thickness, using the reference points that were considered in obtaining the gold standard. The data were analyzed by ANOVA with a significance level of 5%. RESULTS: There was no significant difference in the measurements obtained with different voxel sizes, both for bone height measurements and bone thickness. There was no statistically significant difference in measurements in thickness in comparison to the gold standard. CONCLUSION: When necessary, to measure height and bone thickness, it is possible to recommend voxel images of larger size (0.40 mm) without compromising the quality of the patient's clinical planning.
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The aim of this study was to evaluate and compare the mechanical resistance of platelet-rich fibrin (PRF) membrane when submitted to resistance traction on longitudinal axis. Blood collection of a healthy individual was collected with an amount of 300 mL and divided into 30 tubes containing 10 mL each one. The samples were divided into three groups, according to the g-force protocols: (1) F200 g: (2) F400 g; (3) F800 g. Membranes of each g-force group were divided into subgroups, according to waiting time after centrifugation: (T0) immediate use and (T30) use after 30 min. Considering these intervals of time, the concentrate was removed from tubes and inserted in a PRF metallic box to confection of the membrane. The PRF membranes were submitted to mechanical tension on a universal testing machine and obtained to a resistance force of each membrane. The centrifugation time showed no statistically significant difference for membrane resistance for any force applied (P > 0.05; Student's t-test). For T0 group results demonstrated no influence for membrane resistance (P = 0.357; Student's t-test), therefore T30 showed statistically significant difference (P = 0.040; Student's t-test) for membrane resistance for centrifugation forces applied, with highest value when applied greatest force. The findings suggest that the waiting time for centrifugation could be determined according to demand of application, and for immediate use of the membrane, the centrifugation did not influence the resistance, on the other hand, after 30 min, the application of higher force resulted in a membrane with considerable resistance.
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PURPOSE: To evaluate the influence of low-dose protocols, with different numbers of basis images, on the precision of linear bone measurements in CBCT images. MATERIALS AND METHODS: Five polyurethane mandibles, with different levels of bone resorption, were used in this in vitro study. To obtain the reference standard, landmarks were made in the mandibular superior and buccal cortices, and then a digital caliper was used to measure the bone height and thickness at these regions. CBCT images were obtained with the PaX-i3D (Vatech) unit set at 50 kV, 4 mA, 0.2-mm voxel size and 50 × 50-mm field of view. Keeping these parameters fixed, each mandible was scanned twice, with different protocols: Low dose (L) had an acquisition time of 24 seconds and 720 basis images, and ultralow dose (UL) had an acquisition time of 15 seconds and 450 basis images. Then, measurements of bone height and thickness were performed on the images, using the previously determined landmarks as reference. The obtained data were submitted to statistical analysis, with a significance level of .05. Analysis of variance, Student t test, and intraclass correlation coefficient were employed. RESULTS: Regarding bone height, there were no significant differences between the measurements obtained with the L and UL protocols (P = .8648). Additionally, the L and UL protocols did not differ in relation to the reference standard (P = .8717 and P = .9928, respectively). Likewise, there were no significant differences between the measurements obtained with the L and UL protocols (P = .7969) for bone thickness, nor between these protocols and the gold standard (P = .7455). CONCLUSION: Considering the great demand for precise measurements in implantology, protocols of low-dose radiation can be used without compromising clinical planning.
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Implantes Dentários , Tomografia Computadorizada de Feixe Cônico Espiral , Tomografia Computadorizada de Feixe Cônico , Humanos , Mandíbula/diagnóstico por imagem , Reprodutibilidade dos TestesRESUMO
In some clinical situations, the installation of four or more dental implants may be infeasible. Therefore, the installation of protocol-type prostheses with only three implants may be necessary. The mechanical failures can hinder the success of the rehabilitation treatment. OBJECTIVE: To evaluate and compare the mechanical stress of the metallic prosthetic bar when installed over protocol-type prostheses of three and four implants, and to evaluate the stress on the dental implants. METHODOLOGY: Two three-dimensional human jaw models were created. In one model, four dental implants (P4) were designed and in the other model three dental implants (P3) were designed. In both models, according to the arrangement of the dental implants, prosthetic bars were designed. In a finite element analysis software, compressive forces were applied to the ends of both prosthetic bars, simulating the force application during mastication. Tension and stress dissipation were analyzed on the prosthetic bar and dental implants. RESULTS: Both P4 and P3 protocol-type prostheses supported the force efficiently without breaking the bar or the implants. Force dissipation along the bar was more homogeneous in the P3 prosthetic bar than in P4. In addition, P3 implants received a higher concentration of stress in relation to P4 implants. CONCLUSIONS: Prosthetic bars and dental implants withstand the stress of load application in both four and three implant protocol-type prostheses. Three-implants protocol showed more satisfactory force dissipation than the four-implant protocol.
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OBJECTIVE: The aim of this study was to quantitatively assess the performance of metal artifact reduction (MAR) algorithms on the volume of metal cylinders, considering the influence of materials, positions, and fields of view (FOVs), by using 3 cone beam computed tomography (CBCT) devices (NewTom VGi evo, Picasso Trio, and ProMax 3-D Max). STUDY DESIGN: Nine phantoms containing cylinders of amalgam, copper-aluminum (CuAl) metal alloy, and titanium, combined in up to 3 positions, were scanned by using 2 different FOVs. MATLAB software was used to evaluate the differences between volumes before and after MAR application, and the possible interference of materials, positions, and FOVs. Wilcoxon's test and the Kruskal-Wallis test were used at a level of significance of 5%. RESULTS: In general, images containing amalgam and CuAl showed a significant difference in volume before and after MAR application. However, no significant difference after MAR was observed (P > .05) relative to positions and FOVs. MAR had an impact on the cylinder volumes only in the NewTom VGi evo and ProMax 3-D Max scanners. CONCLUSIONS: The performance of MAR algorithms in volume correction of metal objects is dependent on the materials and the CBCT unit.
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Artefatos , Tomografia Computadorizada de Feixe Cônico Espiral , Algoritmos , Tomografia Computadorizada de Feixe Cônico , Metais , Imagens de FantasmasRESUMO
PURPOSE: The purpose of this study was to evaluate the image quality, diagnostic efficacy, and radiation dose associated with the use of a cadmium telluride (CdTe) detector, compared to charge-coupled device (CCD) and complementary metal oxide semiconductor (CMOS) detectors. MATERIALS AND METHODS: Lateral cephalographs of a phantom (type 1) composed of synthetic polymer filled with water and another phantom (type 2) composed of human skull macerated with polymer coating were obtained with CdTe, CCD, and CMOS detectors. Dosimeters placed on the type 2 phantom were used to measure radiation. Noise levels from each image were also measured. McNamara cephalometric analysis was conducted, the dentoskeletal configurations were assessed, and a subjective evaluation of image quality was conducted. Parametric data were compared via 1-way analysis of variance with the Tukey post-hoc test, with a significance level of 5%. Subjective image quality and dentoskeletal configuration were described qualitatively. RESULTS: A statistically significant difference was found among the images obtained with the 3 detectors (P<0.05), with the lowest noise level observed among the images obtained with the CdTe detector and a higher subjective preference demonstrated for those images. For the cephalometric analyses, no significant difference (P>0.05) was observed, and perfect agreement was seen with regard to the classifications obtained from the images acquired using the 3 detectors. The radiation dose associated with the CMOS detector was higher than the doses associated with the CCD (P<0.05) and CdTe detectors (P<0.05). CONCLUSION: Considering the evaluated parameters, the CdTe detector is recommended for use in clinical practice.
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OBJECTIVES: To create a new phantom design to evaluate the real impact of artefacts caused by titanium on bone structures in cone beam CT images considering different positions and quantity of metals in the dental arch, with and without metal artefact reduction (MAR). METHODS: A three cylindrical polymethyl methacrylate (PMMA) plate phantom was designed containing eight perforations arranged to simulate the lower dental arch in the intermediate plate. Three titanium cylinders were positioned in different locations and quantities to test different clinical conditions and to quantify the impact of the metal artefact around five bone cylinders. Scans were carried out in seven different protocols (Control, A-F) in two cone beam CT devices (OP300 Maxio and Picasso Trio). Eight regions of interest around each cortical and trabecular bone were used to measure the grey value standard deviation corresponding the artefact expression in the Image J software. Both the artefact expression and the MAR effect were assessed using the Wilcoxon, Friedman (Dunn) and Kruskal-Wallis tests (significance level of 5%). RESULTS: For both devices, MAR was statistically efficient only for the protocols E, and F. Protocol F (three metals on the adjacent area of the analysis region) showed higher artefact expression when compared to the others. CONCLUSION: In conclusion, the new phantom design allowed the quantification of the metal artefact expression caused by titanium. The metal artefact expression is higher when more metal objects are positioned in the adjacent bone structures. MAR may not be effective to reduce artefact expression on the adjacencies of those objects for the devices studied.
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Artefatos , Tomografia Computadorizada de Feixe Cônico Espiral , Tomografia Computadorizada de Feixe Cônico , Arco Dental , Metais , Imagens de FantasmasRESUMO
OBJECTIVES: To assess the effect of CBCT detector position (aligned/lateral-offset) on image technical parameters (mean voxel value - MVV, standard deviation of voxel value (SDVV) distribution), comparing peripheral regions of interest (ROIs) to the central ROI in CBCT volumes. METHODS: 40 CBCT volumes of a wax phantom were acquired in six units with aligned and/or lateral-offset detectors: Cranex 3Dx (CRA), Ortophos SL (ORT), Picasso Trio (PIC), Promax 3D Mid (PRO), Scanora 3D (SCA), and X1. Four image-acquisition protocols used an aligned detector, and four a lateral-offset detector. In each volume, 13 ROIs (12 peripheral and 1 central) were evaluated. MVV and SDVV of the peripheral ROI were compared to those of the central ROI in the volume. MVVD (the difference in percentage, between the MVV of a peripheral and the central ROI) was calculated. RESULTS: For aligned-detectors, MVV increased (ORT and PRO) or decreased (CRA and X1) in the ROIs farther from the centre. For lateral-offset detectors, ROIs farther from the centre showed increased MVV. SDVV for most aligned detectors was lower, the nearer the ROI was to the centre. For lateral-offset detectors, it was lower for the peripheral ROIs, except with PIC. Range for MVVD was -32.8% to 22.8% for units with aligned detectors, and -20.7% to 69.5% for lateral-offset detectors. CONCLUSION: Lateral-offset detectors to acquire CBCT images significantly change SDVV distribution within the field-of-view, and lead to MVVD with increased range, compared to aligned detectors. This must be taken in consideration in the clinic, if voxel-value dependent measurements are to be performed.
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Tomografia Computadorizada de Feixe Cônico , Radiografia Dentária Digital , Tomografia Computadorizada de Feixe Cônico Espiral , Humanos , Imagens de FantasmasRESUMO
OBJECTIVE: This survey aimed to assess the effects of coronavirus disease 2019 (COVID-19) on elective and urgency/emergency dental care and dentists concerned. MATERIALS AND METHODS: A web-based survey was performed using Google forms questionnaire sent to dentists in Brazil. Questions included: personal information, type of dental care provided during quarantine, if emergencies increased, the dental office biosafety routine, among others. The levels of concern about the impact of quarantine on dental care and patient oral health conditions and the economic impact on dental practices were evaluated using a 0- to 10-point scale. Statistical analysis included descriptive, percentages, one-way ANOVA, Tukey, and chi-square tests. RESULTS: During quarantine, 64.6% of the dentists attended only urgency/emergency treatments, while 26.1% maintained routine appointments, and 9.3% closed the dental offices. A higher percentage of dentists from the least affected states continued routine dental treatment; dentists were younger and presented a significantly lower level of concern about dental treatments and oral health conditions of their patients. An increase in urgency/emergency procedures was reported by 44.1% of the dentists, mostly due to the unavailability of routine/elective dental care and increased patient anxiety and stress. The main causes of urgency/emergency appointments were toothache, dental trauma, and broken restorations, besides the breakage of orthodontic appliances and temporomandibular disorders. Dentists reported a high level of concern about the economic impact caused by quarantine. CONCLUSIONS: The pandemic/quarantine has negatively affected the clinical routine. Personal protection/hygiene care must be adopted and reinforced by dental professionals/staff to make dental procedures safer.
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OBJECTIVE: To evaluate the effect of Beam Hardening Correction tool (BHC) on Micro-CT images used in the diagnosis of incipient caries lesions. METHODS: Six posterior teeth, with 6 sound proximal surfaces and 6 natural white spot lesions, were scanned using a SkyScan1174 device. Each tooth was imaged 16 times in various acquisition parameters, and reconstructed 3 times applying different levels of BHC (0, 15 and 30%). The 288 datasets were analyzed by three examiners. Gold-standard was obtained by microscopic analyses under polarized light. Area under the ROC curve was obtained for each protocol and the BHC parameter was isolated to individually observe the influence of such parameters of reconstruction. RESULTS: Microtomographic images were accurate for the diagnosis of incipient caries lesions even when the BHC tool was not applied. Accuracy was perfect in 50 % of the protocols without BHC. When adding the artefact correction, the number of protocols that reached perfect accuracy increased to 81.25%. No difference was found between the protocols that used 15 or 30% BHC. Statistically significant differences were found only in four comparisons, and on those the images that had BHC were more accurate. Two non-BHC protocols reached accuracy scores of 0.653 and 0.881, and were improved to perfect accuracy with BHC application. CONCLUSION: The use of the BHC tool should be encouraged when using Micro-CT to detect incipient caries lesions, especially when low-quality parameters aiming less acquisition and processing time are used.
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Cárie Dentária , Artefatos , Cárie Dentária/diagnóstico por imagem , Humanos , Curva ROC , Microtomografia por Raio-XRESUMO
PURPOSE: This study was conducted to evaluate the effect of different spatial resolutions of a photostimulable phosphor plate (PSP) radiography system on the detection of proximal caries lesions. MATERIALS AND METHODS: Forty-five extracted human permanent teeth were radiographed using a PSP system (VistaScan Perio Plus) and scanned at the 4 resolutions (10 lp/mm, 20 lp/mm, 25 lp/mm, and 40 lp/mm) available in the system. Three independent examiners scored the images for the presence and absence of proximal caries lesions using a 5-point scale. The presence or absence of caries was confirmed by histological sections of the examined teeth (defined as the gold standard). Intra- and inter-observer reproducibility was calculated by the weighted kappa test. One-way analysis of variance with the post hoc Tukey test was used to compare the area under the receiver operating characteristic curve for the classifications made with each resolution. RESULTS: For the detection of enamel lesions, the spatial resolution of 10 lp/mm was significantly superior to the other resolutions. However, the spatial resolution did not affect the detection of caries lesions in dentin (P>0.05). CONCLUSION: Spatial resolution may influence the accuracy of the detection of incipient caries lesions in radiographs with PSP plates. Images with low spatial resolution seem to be more appropriate for this purpose.
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OBJECTIVE: To evaluate the influence of rotation degree and field of view (FOV) size on the amount of artefacts produced in cone beam CT (CBCT) images. METHODS: A cylindrical wax utility phantom, with a metallic sample in its interior, was scanned with two FOV sizes (100 x 100 and 40 x 40 mm) and in full (360°) and partial (~180°) rotations. After the acquisitions, images were objectively assessed in the ImageJ software, obtaining the standard deviation in areas around the metal sample. The influence of artefacts produced by the several FOVs and rotation degrees was compared by two-way analysis of variance (α = 0.05). RESULTS: The images obtained with a large FOV presented a higher amount of noise compared to a restricted FOV, both for partial (p = 0.0037) and full (p = 0.0023) rotation degrees. For images obtained with a restricted FOV, full rotation resulted in images with less noise (p = 0.0259). For images obtained with large FOV, there was no statistically significant difference (p = 0.1494) in noise for both rotation protocols. CONCLUSIONS: As there were no significant differences in the amount of artefacts in rotation protocols for large FOVs, the partial rotation can be indicated due to its lower exposure to radiation. For acquiring images with restricted FOVs, the full rotation is recommended.
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Artefatos , Tomografia Computadorizada de Feixe Cônico , Imagens de Fantasmas , RotaçãoRESUMO
OBJECTIVE: The aim of this study was to assess the performance of 2 metal artifact reduction (MAR) algorithms in cone beam computed tomography (CBCT) imaging, considering different materials, metal positions, and fields of view (FOVs). STUDY DESIGN: Nine phantoms containing cylinders of amalgam, copper-aluminum (Cu-Al) alloy, and titanium were scanned by using Picasso Trio and ProMax 3D CBCT units with small and medium FOVs. Scans were made with and without MAR algorithms. The standard deviation (SD) of voxel gray values was measured in the neighborhood of the cylinders. Differences in SD were statistically evaluated for effects of MAR and the other parameters, with a significance level of 5%. RESULTS: Significant differences between images with MAR and those without MAR for both devices (P ≤ .0001) were observed. Amalgam showed the largest artifact expression, followed by Cu-Al and titanium. After correction, differences remained only in Picasso Trio images (Pâ¯=â¯.002). Considering positions, no significant difference in the performance of the MAR algorithm was observed in either device. Considering FOVs, significant differences were observed for ProMax 3D (Pâ¯=â¯.005), with less artifact expression in the medium FOV after MAR correction. CONCLUSIONS: MAR algorithms were effective for artifact reduction despite variation in performance according to device, FOV, and material properties. The position of the metal cylinder within the FOV had no significant effect.
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Artefatos , Tomografia Computadorizada de Feixe Cônico , Materiais Dentários , Algoritmos , Metais , Imagens de FantasmasRESUMO
PURPOSE: To evaluate the effect of different numbers of basis images and the use of metal artifact reduction (MAR) on the production and reduction of artifacts in cone-beam computed tomography images. MATERIALS AND METHODS: An acrylic resin phantom with a metal alloy sample was scanned, with 450 or 720 basis images and with or without MAR. Standard deviation values for the test areas (around the metal object) were obtained as a way of measuring artifact production. Two-way analysis of variance was used with a 5% significance level. RESULTS: There was no significant difference in artifact production among the images obtained with different numbers of basis images without MAR (P=.985). MAR significantly reduced artifact production in the test areas only in the protocol using 720 basis images (P=.017). The protocol using 450 basis images with MAR showed no significant difference in artifact production when compared to the protocol using 720 basis images with MAR (P=.579). CONCLUSION: Protocols with a smaller number of basis images and with MAR activated are preferable for minimizing artifact production in tomographic images without exposing the patient to a greater radiation dose.
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INTRODUCTION: This study evaluated the image quality and the diagnosis of root fractures when using the Zoom Reconstruction tool (J Morita, Kyoto, Japan). METHODS: A utility wax phantom with a metal sample inside was used for objective evaluation, and a mandible with 27 single-rooted teeth (with and without obturation and with and without vertical or horizontal fractures) was used for diagnostic evaluation. The images were acquired in 3 protocols: protocol 1, field of view (FOV) of 4 × 4 cm and a voxel size of 0.08 mm; protocol 2, FOV of 10 × 10 cm and a voxel size of 0.2 mm; and protocol 3, Zoom Reconstruction of images from protocol 2 (FOV of 4 × 4 cm and a voxel size of 0.08 mm). The objective evaluation was achieved by measuring the image noise, and the diagnosis of fractures was performed by 3 evaluators. The area under the receiver operating characteristic curve was used to calculate accuracy, and analysis of variance compared the accuracy and image quality of the protocols. RESULTS: Regarding quality, protocol 1 was superior to protocol 2 (P < .0001) and Zoom Reconstruction (P < .0001). Additionally, images of protocol 2 presented less noise than the Zoom Reconstruction image (P < .0001); however, for diagnosis, Zoom Reconstruction was superior in relation to protocol 2 (P = .011) and did not differ from protocol 1 (P = .228) for the diagnosis of a vertical root fracture in filled teeth. CONCLUSIONS: The Zoom Reconstruction tool allows better accuracy for vertical root fracture detection in filled teeth, making it possible to obtain a higher-resolution image from a lower-resolution examination without having to expose the patient to more radiation.
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Fraturas dos Dentes/diagnóstico por imagem , Raiz Dentária/lesões , Humanos , Processamento de Imagem Assistida por Computador/métodos , Técnicas In Vitro , Intensificação de Imagem Radiográfica/métodos , Radiografia Dentária/métodos , Reprodutibilidade dos Testes , Fraturas dos Dentes/diagnóstico , Raiz Dentária/diagnóstico por imagemRESUMO
X-ray microtomography (microCT) is a nondestructive technique used to assess bone morphometry. For an accurate analysis, it is necessary to segment the bone tissue from the background images, avoiding under- or overestimation of the real bone volume. Thus, segmentation methods for microCT can influence the accuracy of bone morphometry analysis. The purpose of this study was to compare two different image segmentation methods available on microCT software (subjective and objective) regarding to the human bone morphometric analysis. Sixteen samples containing a fixation screws covered by 0.5-1mm of bone were scanned using the SkyScan 1173 scanner. Three examiners segmented the microCT images subjectively and recorded the threshold values. Subsequently, an objective segmentation was also done. The 3D analysis was performed for both images using the valuesâ previously determined in CTAn software. Five bone morphometric parameters were calculated (BV/TV, Tb.Th, Tb.N, Tb.Sp, Conn.Den) and used as dependent variables. ANOVA showed no significant differences between the methods concerning BV/TV (p=0.424), Tb.N (p=0.672), Tb.Th (p=0.183), Tb.Sp (p=0.973) and Conn.Den (p=0.204). Intra- and interobserver agreement ranged from satisfactory to excellent (0.55-1 and 0.546-0.991, respectively). Therefore, results obtained with subjective threshorlding were similar to those obtained with objective segmentation. Since objective segmentation does not have human input and it is a truly objective method, it should be the first choice in microCT studies that concern homogeneity and high resolution human bone sample.