A Pleistocene legacy structures variation in modern seagrass ecosystems.

Distribution of Earth's biomes is structured by the match between climate and plant traits, which in turn shape associated communities and ecosystem processes and services. However, that climate-trait match can be disrupted by historical events, with lasting ecosystem impacts. As Earth's environment changes faster than at any time in human history, critical questions are whether and how organismal traits and ecosystems can adjust to altered conditions. We quantified the relative importance of current environmental forcing versus evolutionary history in shaping the growth form (stature and biomass) and associated community of eelgrass (Zostera marina), a widespread foundation plant of marine ecosystems along Northern Hemisphere coastlines, which experienced major shifts in distribution and genetic composition during the Pleistocene. We found that eelgrass stature and biomass retain a legacy of the Pleistocene colonization of the Atlantic from the ancestral Pacific range and of more recent within-basin bottlenecks and genetic differentiation. This evolutionary legacy in turn influences the biomass of associated algae and invertebrates that fuel coastal food webs, with effects comparable to or stronger than effects of current environmental forcing. Such historical lags in phenotypic acclimatization may constrain ecosystem adjustments to rapid anthropogenic climate change, thus altering predictions about the future functioning of ecosystems.

Ocean current patterns drive the worldwide colonization of eelgrass (Zostera marina).

Currents are unique drivers of oceanic phylogeography and thus determine the distribution of marine coastal species, along with past glaciations and sea-level changes. Here we reconstruct the worldwide colonization history of eelgrass (Zostera marina L.), the most widely distributed marine flowering plant or seagrass from its origin in the Northwest Pacific, based on nuclear and chloroplast genomes. We identified two divergent Pacific clades with evidence for admixture along the East Pacific coast. Two west-to-east (trans-Pacific) colonization events support the key role of the North Pacific Current. Time-calibrated nuclear and chloroplast phylogenies yielded concordant estimates of the arrival of Z. marina in the Atlantic through the Canadian Arctic, suggesting that eelgrass-based ecosystems, hotspots of biodiversity and carbon sequestration, have only been present there for ~243 ky (thousand years). Mediterranean populations were founded ~44 kya, while extant distributions along western and eastern Atlantic shores were founded at the end of the Last Glacial Maximum (~19 kya), with at least one major refuge being the North Carolina region. The recent colonization and five- to sevenfold lower genomic diversity of the Atlantic compared to the Pacific populations raises concern and opportunity about how Atlantic eelgrass might respond to rapidly warming coastal oceans.

Impact of aquaculture and agriculture nutrient sources on macroalgae in a bioassay study.

Eutrophication is a major threat to aquatic ecosystems, because excessive nutrient enrichment may result in the loss of ecosystem services. Fjord systems are specifically under pressure due to nutrient input from land (agriculture) and sea (aquaculture). In this bioassay study, we have analyzed the effect of different nutrient sources, as well as their combination, on growth, nutrient composition and recruitment of habitat-forming and ephemeral macrophytes. We found that agricultural fertilizer increased growth for all algae (except Fucus), while the fish farm effluents mainly increased growth of Ulva. The C:N ratio was hardly affected by the fish farm, but decreased significantly in all algae when agriculture fertilizer was added. Most interestingly, however, distance to the fish farm modulated the algal response to the fertilizer. Our results demonstrate the importance of studying effects of multiple stressors in aquatic ecosystems to sustainably manage the consequences of anthropogenic impacts.

No support for cryptic choice by ovarian fluid in an external fertilizer.

Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior-the Arctic charr (Salvelinus alpinus). We physically separated the OF from the eggs of reproductively active females and reintroduced either their own OF or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split-brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual OFs. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of OF treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that OF plays an important role as medium for cryptic female choice in charr. Power analyses revealed that our sample size is large enough to detect medium-sized changes in relative paternity (medium-sized effect sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about OF's potential influence on paternity under sperm competition-even in charr.

Effects of aquaculture fallowing on the recovery of macrofauna communities.

The fallowing period is a management measure in aquaculture where the production is paused for a few months to reduce the impact on the benthic environment. We studied the effects of different fallowing periods on the recovery of macrofauna at two salmon farms in Norway. The macrofauna at the farm stations were characterised by high abundances of opportunistic taxa (e.g. Capitella spp.), low diversity and significantly different community structure compared to reference sites. The fallowing initiated macrofauna recovery at both farm stations, indicated by a decline of dominant opportunistic taxa after 2months. Significant changes in taxa composition occurred only after 6months, although indications of disturbance were still evident. Surprisingly, no corresponding spatial or temporal differences were found in the sediment parameters such as redox, TOC and pH. The results suggest that macrofauna is a more sensitive indicator and that the seasonal timing of fallowing may affect recovery dynamics.