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1.
Int J Food Sci Nutr ; 66(2): 222-9, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25657100

RESUMO

Lutein and docosahexaenoic acid (DHA) are associated with the prevention of age-related macular degeneration (AMD). Since microalgae are potent natural sources of these nutrients, their nutritional value should be evaluated based on the bioavailability of lutein and DHA for the retina via the plasmatic compartment. In this study, quail were fed for 5 months either with a diet supplemented or deprived with microalgae rich in lutein and DHA. In the microalgae-fed group, the retinal concentrations of lutein and zeaxanthin gradually increased whereas in plasma, these compounds started to increase from the first month of supplementation. We also observed a significant increase in retinal and plasmatic levels of DHA in the microalgae-fed group. In conclusion, the plasmatic and retinal contents of lutein and DHA were significantly increased in quail fed with lutein- and DHA-rich microalgae. Food fortification with microalgae may be an innovative way to increase lutein and DHA consumption in humans.


Assuntos
Suplementos Nutricionais , Ácidos Docosa-Hexaenoicos/metabolismo , Luteína/metabolismo , Degeneração Macular , Microalgas/química , Retina/metabolismo , Animais , Disponibilidade Biológica , Dieta , Ácidos Docosa-Hexaenoicos/sangue , Ácidos Docosa-Hexaenoicos/farmacocinética , Humanos , Luteína/sangue , Luteína/farmacocinética , Degeneração Macular/metabolismo , Degeneração Macular/prevenção & controle , Modelos Animais , Codorniz , Zeaxantinas/metabolismo
2.
Sci Rep ; 14(1): 9365, 2024 04 23.
Artigo em Inglês | MEDLINE | ID: mdl-38654026

RESUMO

Strategies against the opportunistic fungal pathogen Candida albicans based on probiotic microorganisms represent a promising alternative to traditional antifungals. Here, we investigated the effects of Lactobacillaceae isolates from fermented foods or the human vagina, alone or in combination with the probiotic yeast Saccharomyces cerevisiae CNCM I-3856, against C. albicans in vitro. Nine out of nineteen tested strains of Lactobacillaceae inhibited growth of C. albicans with inhibition zones of 1-3 mm in spot assays. Five out of nineteen lactobacilli tested as such or in combination with S. cerevisiae CNCM I-3856 also significantly inhibited C. albicans hyphae formation, including Limosilactobacillus fermentum LS4 and L. fermentum LS5 resulting in respectively 62% and 78% hyphae inhibition compared to the control. Thirteen of the tested nineteen lactobacilli aggregated with the yeast form of C. albicans, with Lactiplantibacillus carotarum AMBF275 showing the strongest aggregation. The aggregation was enhanced when lactobacilli were combined with S. cerevisiae CNCM I-3856. No significant antagonistic effects were observed between the tested lactobacilli and S. cerevisiae CNCM I-3856. The multifactorial activity of Lactobacillaceae strains alone or combined with the probiotic S. cerevisiae CNCM I-3856 against C. albicans without antagonistic effects between the beneficial strains, paves the way for developing consortium probiotics for in vivo applications.


Assuntos
Candida albicans , Lactobacillus , Probióticos , Saccharomyces cerevisiae , Candida albicans/efeitos dos fármacos , Saccharomyces cerevisiae/crescimento & desenvolvimento , Saccharomyces cerevisiae/efeitos dos fármacos , Probióticos/farmacologia , Lactobacillus/fisiologia , Humanos , Hifas/efeitos dos fármacos , Hifas/crescimento & desenvolvimento , Antibiose , Feminino , Vagina/microbiologia
3.
Appl Environ Microbiol ; 76(9): 2846-55, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20208019

RESUMO

The beneficial effects of Bifidobacterium are partly due to its immunostimulatory properties. These immunostimulatory properties may be linked to the presence of unmethylated CpG motifs specific to bacterial DNA, which may induce a TH1 response by activating Toll-like receptors (TLR). Using in silico analyses, PCR amplification, and dot blotting, we characterized the CpG content of various bifidobacterial strains and evaluated the immunostimulatory properties and genomic heterogeneity of these motifs in the genus. Our in silico study, based on entire genome sequences from five bifidobacterial strains, showed that Bifidobacterium genomes contain numerous CpG motifs, including 5'-purine-purine-CG-pyrimidine-pyrimidine-3' and 5'-purine-TCG-pyrimidine-pyrimidine-3' motifs, and biologically active sequences previously identified in lactic acid bacteria. We identified four CpG-rich sequences with Bifidobacterium longum NCC2705. Two sequences with a percent G+C of about 68% included 14 and 16 CpG motifs. Two sequences with a percent G+C of about 60% included 16 and 6 CpG motifs. These sequences induce the production of monocyte chemoattractant protein 1 (MCP-1) and tumor necrosis factor alpha (TNF-alpha) through a pattern of TLR9 stimulation on RAW 264.7 macrophages. No link could be established between their immunostimulatory properties, the number of CpG motifs, and percent G+C. We investigated inter- and intraspecies heterogeneity in 71 strains of various origins. These sequences were highly conserved in the genus. No link was found between the presence of the CpG-rich sequence and the origin of the strains (healthy, allergic, or preterm infants). The high frequency of CpG motifs in the DNA of Bifidobacterium may play an important role in the immunostimulatory properties of commensal or probiotic bifidobacterial strains.


Assuntos
Adjuvantes Imunológicos/farmacologia , Bifidobacterium/genética , Ilhas de CpG , DNA Bacteriano/farmacologia , Fosfatos de Dinucleosídeos/farmacologia , Adjuvantes Imunológicos/genética , Sequência de Bases , DNA Bacteriano/química , Humanos , Lactente , Receptores Toll-Like/metabolismo , Fator de Necrose Tumoral alfa/farmacologia
4.
Nutrients ; 12(8)2020 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-32722250

RESUMO

Bacterial vaginosis and vulvovaginal candidiasis are common causes of impaired health and quality of life for women. Although antimicrobial agents remain the main strategy for the treatment of vaginal infections, their repeated use involves high rates of resistance and recurrence. Alternative approaches such as probiotics are studied. Saccharomyces cerevisiae CNCM I-3856 already demonstrated beneficial effects in experimental models of vaginal infections. This randomized, double-blind, placebo-controlled clinical study was performed to evaluate the recovery of S. cerevisiae CNCM I-3856 in vaginal samples in healthy women after oral consumption. Sixty healthy women were randomized to receive a daily dose of S. cerevisiae CNCM I-3856 or a placebo for 4 weeks. Subcultures and quantitative polymerase chain reaction (qPCR) were used to detect the strain in vaginal and stool samples. A safety assessment was carried out throughout the study. Fifty-seven women completed the study. Over the 4-week supplementation phase, S. cerevisiae CNCM I-3856 has been detected in the vaginal samples of 21% of women (n = 4/19) in the 500 mg Probiotic group and 16% of women (n = 3/19) in the 1000 mg Probiotic group. The strain was detected in the faeces of 90% of women consuming the probiotic. This is the first clinical study demonstrating the migration of yeast from intestine to vagina where it may exert its benefits.


Assuntos
Candidíase Vulvovaginal/prevenção & controle , Probióticos/administração & dosagem , Saccharomyces cerevisiae , Vaginose Bacteriana/prevenção & controle , Administração Oral , Adulto , Candidíase Vulvovaginal/microbiologia , Método Duplo-Cego , Fezes/microbiologia , Feminino , Voluntários Saudáveis , Humanos , Vagina/microbiologia , Vaginose Bacteriana/microbiologia
5.
Front Microbiol ; 9: 1537, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30042756

RESUMO

Preventive actions of probiotics as antidiarrheal agents are well documented, but their mechanisms are poorly understood. Two selected probiotics, Bacillus subtilis CU1 and Lactobacillus plantarum CNCM I-4547, were tested in mouse experimental models of diarrhea and the possible mechanisms of action were investigated. Diarrhea was induced in mice by oral castor oil administration or by i.v. injection of lipopolysaccharide (LPS) of Salmonella enteritis. The antidiarrheal drug loperamide was used as control. Fecal water excretion was quantified for 2 h and paracellular permeability and electrical parameters of the colon were assessed in Ussing chambers. The expression of colonic exchangers or channels and of Toll-like receptor 4 (TLR4) was assessed by immunohistochemistry. Prophylactic treatment with B. subtilis CU1 or with L. plantarum CNCM I-4547 reduced LPS-induced diarrhea. The reduction of water excretion was in the same range as those induced by loperamide. In the castor oil model, this effect was only observed with B. subtilis CU1. The two probiotic treatments abolished the increase in paracellular permeability induced by LPS, but not by castor oil. However, only L. plantarum CNCM I-4547 treatment decreased the colonic expression of TLR-4. After B. subtilis CU1, colonic expression of cystic fibrosis transmembrane conductance regulator (CFTR) was reduced and that of Na+/H+ exchanger 3 (NHE3) increased. B. subtilis CU1 may increase the capacity of the colon to absorb excess of water in diarrheic conditions by acting on CFTR and NHE3 expression. The two probiotics strains showed an impact on diarrhea through limitation of water excretion that may involve paracellular permeability or electrolyte transport for L. plantarum CNCM I-4547 and B. subtilis CU1 respectively.

6.
FEMS Microbiol Ecol ; 79(1): 192-202, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22029421

RESUMO

Faecal microbiota of healthy infant displays a large abundance of Bifidobacterium spp. and Bacteroides spp. Although some studies have reported an association between these two genera and allergy, these findings remain a subject of debate. Using a gnotobiotic mouse model of cow's milk allergy, we investigated the impact of an infant gut microbiota ­ mainly composed of Bifidobacterium and Bacteroides spp. ­ on immune activation and allergic manifestations. The transplanted microbiota failed to restore an ileal T-cell response similar to the one observed in conventional mice. This may be due to the low bacterial translocation into Peyer's patches in gnotobiotic mice. The allergic response was then monitored in germ-free, gnotobiotic, and conventional mice after repeated oral sensitization with whey proteins and cholera toxin. Colonized mice displayed a lower drop of rectal temperature upon oral challenge with b-lactoglobulin, lower plasma mMCP-1, and lower anti-BLG IgG1 than germ-free mice. The foxp3 gene was highly expressed in the ileum of both colonized mice that were protected against allergy. This study is the first demonstration that a transplanted healthy infant microbiota mainly composed of Bifidobacterium and Bacteroides had a protective impact on sensitization and food allergy in mice despite altered T-cell response in the ileum.


Assuntos
Íleo/microbiologia , Imunidade Celular , Metagenoma/fisiologia , Hipersensibilidade a Leite/microbiologia , Leite/efeitos adversos , Animais , Bacteroides/fisiologia , Bifidobacterium/fisiologia , Modelos Animais de Doenças , Fezes/microbiologia , Trato Gastrointestinal , Vida Livre de Germes , Humanos , Íleo/imunologia , Imunoglobulina G/sangue , Lactente , Camundongos , Camundongos Endogâmicos C3H , Hipersensibilidade a Leite/imunologia , Hipersensibilidade a Leite/prevenção & controle , Linfócitos T/imunologia , Linfócitos T Reguladores
7.
FEMS Microbiol Ecol ; 76(1): 133-44, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21223329

RESUMO

Studies suggesting that the development of atopy is linked to gut microbiota composition are inconclusive on whether dysbiosis precedes or arises from allergic symptoms. Using a mouse model of cow's milk allergy, we aimed at investigating the link between the intestinal microbiota, allergic sensitization, and the severity of symptoms. Germ-free and conventional mice were orally sensitized with whey proteins and cholera toxin, and then orally challenged with ß-lactoglobulin (BLG). Allergic responses were monitored with clinical symptoms, plasma markers of sensitization, and the T-helper Th1/Th2/regulatory-T-cell balance. Microbiota compositions were analysed using denaturing gradient gel electrophoresis and culture methods. Germ-free mice were found to be more responsive than conventional mice to sensitization, displaying a greater reduction of rectal temperature upon challenge, higher levels of blood mouse mast cell protease-1 (mMCP-1) and BLG-specific immunoglobulin G1 (IgG1), and a systemic Th2-skewed response. This may be explained by a high susceptibility to release mMCP-1 even in the presence of low levels of IgE. Sensitization did not alter the microbiota composition. However, the absence of or low Staphylococcus colonization in the caecum was associated with high allergic manifestations. This work demonstrates that intestinal colonization protects against oral sensitization and allergic response. This is the first study to show a relationship between alterations within the subdominant microbiota and severity of food allergy.


Assuntos
Ceco/microbiologia , Metagenoma/imunologia , Hipersensibilidade a Leite/imunologia , Proteínas do Leite/imunologia , Animais , Bovinos , Ceco/imunologia , Quimases/sangue , Quimases/imunologia , DNA Bacteriano/isolamento & purificação , Modelos Animais de Doenças , Fezes/microbiologia , Vida Livre de Germes , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Lactoglobulinas/farmacologia , Camundongos , Camundongos Endogâmicos C3H , Hipersensibilidade a Leite/sangue , Baço/citologia , Baço/imunologia , Proteínas do Soro do Leite
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