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1.
Appl Microbiol Biotechnol ; 107(7-8): 2027-2037, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36820899

RESUMO

The different organisms, ranging from plants to bacteria, and viruses that dwell on built cultural heritage can be passive or active participants in conservation processes. For the active participants, particular attention is generally given to organisms that play a positive role in bioprotection, bioprecipitation, bioconsolidation, bioremediation, biocleaning, and biological control and to those involved in providing ecosystem services, such as reducing temperature, pollution, and noise in urban areas. The organisms can also evolve or mutate in response to changes, becoming tolerant and resistant to biocidal treatments or acquiring certain capacities, such as water repellency or resistance to ultraviolet radiation. Our understanding of the capacities and roles of these active organisms is constantly evolving as bioprotection/biodeterioration, and biotreatment studies are conducted and new techniques for characterizing species are developed. This brief review article aims to shed light on interesting research that has been abandoned as well as on recent (some ongoing) studies opening up new scopes of research involving a wide variety of organisms and viruses, which are likely to receive more attention in the coming years. KEY POINTS: • Organisms and viruses can be active or passive players in heritage conservation • Biotreatment and ecosystem service studies involving organisms and viruses are shown • Green deal, health, ecosystem services, and global change may shape future research.


Assuntos
Ecossistema , Vírus , Humanos , Raios Ultravioleta , Plantas , Bactérias
2.
Appl Microbiol Biotechnol ; 106(11): 4297-4314, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35596787

RESUMO

The irregular damp dark staining on the stonework of a salt-contaminated twelfth century granite-built chapel is thought to be related to a non-homogeneous distribution of salts and microbial communities. To enhance understanding of the role of microorganisms in the presence of salt and damp stains, we determined the salt content and identified the microbial ecosystem in several paving slabs and inner wall slabs (untreated and previously bio-desalinated) and in the exterior surrounding soil. Soluble salt analysis and culture-dependent approaches combined with archaeal and bacterial 16S rRNA and fungal ITS fragment as well as with the functional genes nirK, dsr, and soxB long-amplicon MinION-based sequencing were performed. State-of-the-art technology was used for microbial identification, providing information about the microbial diversity and phylogenetic groups present and enabling us to gain some insight into the biological cycles occurring in the community key genes involved in the different geomicrobiological cycles. A well-defined relationship between microbial data and soluble salts was identified, suggesting that poorly soluble salts (CaSO4) could fill the pores in the stone and lead to condensation and dissolution of highly soluble salts (Ca(NO3)2 and Mg(NO3)2) in the thin layer of water formed on the stonework. By contrast, no direct relationship between the damp staining and the salt content or related microbiota was established. Further analysis regarding organic matter and recalcitrant elements in the stonework should be carried out. KEY POINTS : • Poorly (CaSO4) and highly (Ca(NO3)2, Mg(NO3)2) soluble salts were detected • Halophilic and mineral weathering microorganisms reveal ecological impacts of salts • Microbial communities involved in nitrate and sulfate cycles were detected.


Assuntos
Bactérias , Microbiota , Bactérias/genética , Filogenia , RNA Ribossômico 16S/genética , Sais , Dióxido de Silício , Solo , Microbiologia do Solo
3.
Adicciones ; 34(3): 227-234, 2022 Jul 01.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-33768256

RESUMO

Substance use disorders (SUD) treatment centers are an optimal setting for delivering smoking cessation interventions (SCI). This study aimed to examine the adoption of SCI in SUD treatment centers in Catalonia (Spain) as well as to assess their managers' views on the appropriateness and feasibility of providing SCI. Managers directly in charge of SUD treatment centers (n = 57) answered a 30-item on-line questionnaire. Data was obtained of 50 centers (87.7% response rate). Forty-six per cent of the centers provided some kind of SCI, but only 4.8% of the new patients were treated for smoking cessation. Managers reported that 73.3% of mental health professionals working in SUD centers had not been trained in SCI. Sixty-four per cent of managers agreed that all health professionals should deliver SCI. Those centers offering SCI attended more patients and were more likely to have professionals trained in SCI than those not offering SCI. The implementation of SCI in SUD treatment centers in Catalonia was suboptimal. Continuing education and training should be provided for all health professionals working in SUD centers. Not systematically delivering SCI to patients in treatment for other SUD means missing opportunities to reduce health and economic costs while perpetuating a smoking culture.


Los centros de tratamiento de drogodependencias son un recurso óptimo para realizar intervenciones para la cesación tabáquica (ICT). El objetivo de este estudio fue examinar la implementación de ICT en la Red de centros de Atención a las Drogodependencias (CAS) de Cataluña, así como evaluar las opiniones sobre la adecuación y viabilidad de la provisión de ICT. Los responsables de los CAS (n = 57) contestaron un cuestionario on-line compuesto por 30 ítems. Se obtuvieron datos de 50 centros (87,7% tasa de respuesta). El 46% de los CAS ofrecía algún tipo de ICT, pero sólo un 4,8% de los nuevos pacientes eran tratados para dejar de fumar. Además, los responsables informaron que el 73,3% de los profesionales que trabajaban en los CAS no había recibido formación en ICT. El 64% de los responsables estaba de acuerdo que todos los profesionales deberían realizar ICT. Aquellos centros que ofrecían ICT visitaban más pacientes y era más probable que tuviesen profesionales formados en ICT, comparado con los centros que no ofrecían ICT. La implementación de ICT en los CAS de Cataluña era subóptima. Se debería facilitar formación continuada a los profesionales de los CAS. No intervenir sobre el consumo de tabaco en pacientes en tratamiento por otras drogodependencias significa perder oportunidades para reducir costes en salud y económicos mientras perpetuamos una cultura fumadora.


Assuntos
Comportamento Aditivo , Abandono do Hábito de Fumar , Transtornos Relacionados ao Uso de Substâncias , Terapia Comportamental , Humanos , Espanha , Transtornos Relacionados ao Uso de Substâncias/terapia
4.
Salud Colect ; 20: e4665, 2024 02 29.
Artigo em Inglês, Espanhol | MEDLINE | ID: mdl-38427347

RESUMO

This research aims to analyze the breastfeeding experiences of incarcerated mothers in the prisons of the Spanish penitentiary system. Additionally, it explores whether these mothers have perceived practices related to obstetric violence during pregnancy, childbirth, and the postpartum period. An exploratory-descriptive study was conducted using a qualitative approach and a critical ethnographic method. Fieldwork, including participant observation and semi-structured interviews, was carried out between December 2021 and April 2022. The study involved 30 adult women from Africa, Europe, Eastern Europe, and Latin America, all serving sentences with their infants in Mother Units located in the Spanish cities of Alicante, Barcelona, Madrid, and Seville. The main findings highlight the need for penitentiary policies with a gender and feminist perspective. These policies should aim to eliminate severe inequalities and discriminations faced by incarcerated women while protecting the basic rights of both mothers and infants.


Esta investigación tiene como objetivo analizar la experiencia con respecto a la lactancia materna de las madres encarceladas en las prisiones del sistema penitenciario español, así como estudiar si han percibido prácticas que aludan a la violencia obstetricia durante la gestación, el parto y el puerperio. Se realizó un estudio exploratorio-descriptivo con abordaje cualitativo y método etnográfico crítico. Entre diciembre de 2021 y abril de 2022, se efectuó el trabajo de campo con observación participante y entrevistas semiestructuradas a 30 de las mujeres mayores de edad procedentes de África, Europa, Europa del Este y Latinoamérica, que se encontraban cumpliendo condena junto a sus criaturas en las Unidades de Madres de las ciudades españolas de Alicante, Barcelona, Madrid y Sevilla. Las principales conclusiones señalan la necesidad de aplicar políticas penitenciarias con perspectiva de género y feminista, que consigan erradicar las graves desigualdades y discriminaciones que sufren las mujeres encarceladas y que sirvan para proteger los derechos básicos de madres y criaturas.


Assuntos
Prisioneiros , Prisões , Adulto , Gravidez , Feminino , Humanos , Mães , Aleitamento Materno , Parto , Pesquisa Qualitativa
5.
Microb Cell ; 8(5): 91-105, 2021 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-33981761

RESUMO

An innovative methodology is proposed, based on applied biotechnology to the recovery of altered stonework: the "dry biocleaning", which envisages the use of dehydrated microbial cells without the use of free water or gel-based matrices. This methodology can be particularly useful for the recovery of highly-ornamented stoneworks, which cannot be treated using the conventional cleaning techniques. The experimental plan included initial laboratory tests on Carrara marble samples, inoculated with dehydrated Saccharomyces cerevisiae yeast cells, followed by on-site tests performed on "Quattro Fontane" (The Four Fountains), a travertine monumental complex in Rome (Italy), on altered highly ornamented areas of about 1,000 cm2. The mechanism is based on the spontaneous re-hydration process due to the environmental humidity and on the metabolic fermentative activity of the yeast cells. Evaluation by physical-chemical analyses, after 18 hours of the biocleaning, confirmed a better removal of salts and pollutants, compared to both nebulization treatment and control tests (without cells). The new proposed on-site dry biocleaning technique, adopting viable yeast cells, represents a promising method that can be further investigated and optimized for recovering specific altered Cultural Heritage stoneworks.

6.
Salud colect ; 20: 4665-4665, 2024. tab
Artigo em Espanhol | LILACS-Express | LILACS | ID: biblio-1560485

RESUMO

RESUMEN Esta investigación tiene como objetivo analizar la experiencia con respecto a la lactancia materna de las madres encarceladas en las prisiones del sistema penitenciario español, así como estudiar si han percibido prácticas que aludan a la violencia obstetricia durante la gestación, el parto y el puerperio. Se realizó un estudio exploratorio-descriptivo con abordaje cualitativo y método etnográfico crítico. Entre diciembre de 2021 y abril de 2022, se efectuó el trabajo de campo con observación participante y entrevistas semiestructuradas a 30 de las mujeres mayores de edad procedentes de África, Europa, Europa del Este y Latinoamérica, que se encontraban cumpliendo condena junto a sus criaturas en las Unidades de Madres de las ciudades españolas de Alicante, Barcelona, Madrid y Sevilla. Las principales conclusiones señalan la necesidad de aplicar políticas penitenciarias con perspectiva de género y feminista, que consigan erradicar las graves desigualdades y discriminaciones que sufren las mujeres encarceladas y que sirvan para proteger los derechos básicos de madres y criaturas.


ABSTRACT This research aims to analyze the breastfeeding experiences of incarcerated mothers in the prisons of the Spanish penitentiary system. Additionally, it explores whether these mothers have perceived practices related to obstetric violence during pregnancy, childbirth, and the postpartum period. An exploratory-descriptive study was conducted using a qualitative approach and a critical ethnographic method. Fieldwork, including participant observation and semi-structured interviews, was carried out between December 2021 and April 2022. The study involved 30 adult women from Africa, Europe, Eastern Europe, and Latin America, all serving sentences with their infants in Mother Units located in the Spanish cities of Alicante, Barcelona, Madrid, and Seville. The main findings highlight the need for penitentiary policies with a gender and feminist perspective. These policies should aim to eliminate severe inequalities and discriminations faced by incarcerated women while protecting the basic rights of both mothers and infants.

7.
Artigo em Inglês, Espanhol | IBECS (Espanha) | ID: ibc-206332

RESUMO

Los centros de tratamiento de drogodependencias son un recurso óptimo para realizar intervenciones para la cesación tabáquica (ICT). Elobjetivo de este estudio fue examinar la implementación de ICT en laRed de centros de Atención a las Drogodependencias (CAS) de Cataluña, así como evaluar las opiniones sobre la adecuación y viabilidad de laprovisión de ICT. Los responsables de los CAS (n = 57) contestaron uncuestionario on-line compuesto por 30 ítems. Se obtuvieron datos de 50centros (87,7% tasa de respuesta). El 46% de los CAS ofrecía algún tipode ICT, pero sólo un 4,8% de los nuevos pacientes eran tratados paradejar de fumar. Además, los responsables informaron que el 73,3% delos profesionales que trabajaban en los CAS no había recibido formación en ICT. El 64% de los responsables estaba de acuerdo que todos losprofesionales deberían realizar ICT. Aquellos centros que ofrecían ICTvisitaban más pacientes y era más probable que tuviesen profesionalesformados en ICT, comparado con los centros que no ofrecían ICT. La implementación de ICT en los CAS de Cataluña era subóptima. Se deberíafacilitar formación continuada a los profesionales de los CAS. No venir sobre el consumo de tabaco en pacientes en tratamiento por otrasdrogodependencias significa perder oportunidades para reducir costesen salud y económicos mientras perpetuamos una cultura fumadora. (AU)


Substance use disorders (SUD) treatment centers are an optimal setting for delivering smoking cessation interventions (SCI). This studyaimed to examine the adoption of SCI in SUD treatment centers inCatalonia (Spain) as well as to assess their managers’ views on theappropriateness and feasibility of providing SCI. Managers directly incharge of SUD treatment centers (n = 57) answered a 30-item on-linequestionnaire. Data was obtained of 50 centers (87.7% response rate).Forty-six per cent of the centers provided some kind of SCI, but only4.8% of the new patients were treated for smoking cessation. Managers reported that 73.3% of mental health professionals working inSUD centers had not been trained in SCI. Sixty-four per cent of managers agreed that all health professionals should deliver SCI. Thosecenters offering SCI attended more patients and were more likely tohave professionals trained in SCI than those not offering SCI. Theimplementation of SCI in SUD treatment centers in Catalonia wassuboptimal. Continuing education and training should be providedfor all health professionals working in SUD centers. Not systematically delivering SCI to patients in treatment for other SUD means missingopportunities to reduce health and economic costs while perpetuating a smoking culture. (AU)


Assuntos
Humanos , Abandono do Uso de Tabaco/métodos , Centros de Tratamento de Abuso de Substâncias/estatística & dados numéricos , Inquéritos e Questionários/estatística & dados numéricos , Estudos Transversais
8.
Res Microbiol ; 167(9-10): 757-765, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27639669

RESUMO

Research on biotechnology applications for cultural heritage restoration has shown how microorganisms can be efficient at cleaning particularly complex or ingrained substances through the process called "biocleaning". Bacteria are able to synthesize groups of specific enzymes for the degradation of complex materials present on artwork. Biocleaning has been shown to be less hazardous than some traditional mechanical or chemical techniques for the artwork, to be environmentally-friendly and safe for restorers to use. In order to improve our knowledge of the metabolic mechanisms involved in biocleaning, we analyzed the relationship between the genome and phenome of Pseudomonas stutzeri 5190 in order to identify and confirm the benefits and drawbacks of this bacterium used on on-site artwork as a biocleaning agent. Main phenotype microarray (PM) assays showed that P. stutzeri 5190 was able to use: i) 51 of the 190 carbon sources tested, where 32 were used efficiently, among which there were six amino acids (l-proline, l-alanine, d-alanine, l-glutamic acid, l-asparagine and l-glutamine); ii) 74 of the 95 nitrogen sources tested, where 50 compounds were used efficiently, among which were 28 amino acids and the inorganic nitrate and nitrite compounds, supporting the hypothesis of the strain's ability to remove nitrate salt efflorescence from frescoes. Furthermore, high tolerance to osmotic stress, to basic pH and to toxic compounds was revealed by PM. Putative genes compatible with these phenotypes are described.


Assuntos
Poluentes Ambientais/metabolismo , Genótipo , Análise em Microsséries , Fenótipo , Pseudomonas stutzeri/genética , Pseudomonas stutzeri/metabolismo , Biotransformação , Carbono/metabolismo , Tolerância a Medicamentos , Concentração de Íons de Hidrogênio , Nitrogênio/metabolismo
9.
Clín. salud ; 28(2): 65-70, jul. 2017. tab, graf
Artigo em Espanhol | IBECS (Espanha) | ID: ibc-163959

RESUMO

En el presente trabajo se estudian y analizan variables sociodemográficas, clínicas y asistenciales incluidas en los partes de interconsulta (PIC) que traen los pacientes adultos durante un periodo de 15 días recogidos de forma consecutiva en los meses de marzo a junio del 2015 en tres centros de Salud Mental de Madrid (CSM Colmenar Viejo, CSM Vallecas Villa y CSM Villaverde). El estudio estadístico mediante el análisis de variables descriptivas y variables dicotómicas muestra los siguientes resultados: dos tercios de las derivaciones a salud mental son a psiquiatría y un tercio están destinadas a psicología clínica; el tiempo medio de espera para la primera consulta en psicología es de 74 días frente a 38 días en psiquiatría; el 31% de las derivaciones son con carácter preferente con un tiempo medio de demora de 28 días y la mayor parte de las mismas se dirigen a la especialidad de psiquiatría; las principales patologías derivadas son trastornos de ansiedad, cuadros adaptativos y depresivos; el 46.7% de los pacientes derivados a salud mental toman ya algún psicofármaco; las derivaciones a psicología clínica se realizan con pacientes más jóvenes y menos medicados, con menor carácter de urgencia y con menos especificaciones sobre información diagnóstica y farmacológica en los PIC que las derivaciones a psiquiatría. Estos resultados son en su mayoría coherentes con los encontrados en estudios previos, aunque faltan investigaciones que incluyan varios CSM o analicen la información cualitativa de los PIC de derivación


In this paper, we analyze the sociodemographic, clinical, and health care variables included in the interconsultation reports (PIC) that bring adult patients over a period of 15 days, collected consecutively in the months of March through June 2015 from three mental health centers in Madrid (Colmenar Viejo CSM, Villaverde CSM, and Vallecas Villa CSM). The statistical analysis of data, including analysis of descriptive variables and dichotomous variables, shows the following results: two thirds of referrals are transfers to psychiatry and one third are transfers to clinical psychology; mean time in the waiting list is 74 days for clinical psychology consultation and 38 days for psychiatry consultation; preferential referrals are 31%, with 28 days of mean time in waiting list, being mostly intended for psychiatry; main referred pathologies are anxiety and adaptive and depressive disorders; 46.7% of patients transferred to mental health were already taking some psychiatric medication; clinical psychology referrals involve significantly younger and less medicated patients, significantly less preferential, and with fewer diagnostic and pharmacologic specifications in the PIC than psychiatry referrals. These results are consistent with previous studies, although we have not found other studies that include more than one mental health center, or analyze qualitative information contained in the inter-consultation reports


Assuntos
Humanos , Adulto , Adulto Jovem , Atenção Primária à Saúde/organização & administração , Hospitais Psiquiátricos/organização & administração , Encaminhamento e Consulta/organização & administração , Transtornos Mentais/epidemiologia , Prontuários Médicos/estatística & dados numéricos , Tempo para o Tratamento/estatística & dados numéricos , Psicotrópicos/uso terapêutico
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