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1.
Euro Surveill ; 21(15)2016 04 14.
Artigo em Inglês | MEDLINE | ID: mdl-27105043

RESUMO

An approach is proposed for environmental surveillance of poliovirus by concentrating sewage samples with tangential flow filtration (TFF) followed by deep sequencing of viral RNA. Subsequent to testing the method with samples from Finland, samples from Pakistan, a country endemic for poliovirus, were investigated. Genomic sequencing was either performed directly, for unbiased identification of viruses regardless of their ability to grow in cell cultures, or after virus enrichment by cell culture or immunoprecipitation. Bioinformatics enabled separation and determination of individual consensus sequences. Overall, deep sequencing of the entire viral population identified polioviruses, non-polio enteroviruses, and other viruses. In Pakistani sewage samples, adeno-associated virus, unable to replicate autonomously in cell cultures, was the most abundant human virus. The presence of recombinants of wild polioviruses of serotype 1 (WPV1) was also inferred, whereby currently circulating WPV1 of south-Asian (SOAS) lineage comprised two sub-lineages depending on their non-capsid region origin. Complete genome analyses additionally identified point mutants and intertypic recombinants between attenuated Sabin strains in the Pakistani samples, and in one Finnish sample. The approach could allow rapid environmental surveillance of viruses causing human infections. It creates a permanent digital repository of the entire virome potentially useful for retrospective screening of future discovered viruses.


Assuntos
Citometria de Fluxo/métodos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Poliovirus/genética , Poliovirus/isolamento & purificação , Vigilância da População/métodos , Esgotos/virologia , Monitoramento Ambiental/métodos , Finlândia , Humanos , Metagenômica/métodos , Técnicas Microbiológicas/métodos , Paquistão , RNA Viral/genética , Ultrafiltração/métodos
2.
Duodecim ; 132(2): 165-71, 2016.
Artigo em Fi | MEDLINE | ID: mdl-26939490

RESUMO

BACKGROUND: Recurrent cases of gastroenteritis occurred in a small hotel. The causative agent of disease could not be detected. MATERIAL AND METHODS: The cause and the source of the disease were established through epidemiological investigations and laboratory diagnosis. RESULTS: The causative agent of the disease was norovirus GI.3. Norovirus GI was detected in the water from the well and on surfaces at the hotel. CONCLUSIONS: Both epidemiological investigations and laboratory diagnostics are needed in resolving epidemics. Continuous development of laboratory methods is important.


Assuntos
Infecções por Caliciviridae/epidemiologia , Infecções por Caliciviridae/virologia , Surtos de Doenças , Gastroenterite/epidemiologia , Gastroenterite/virologia , Habitação , Norovirus/isolamento & purificação , Microbiologia da Água , Humanos , Recidiva
3.
J Infect Dis ; 210 Suppl 1: S347-52, 2014 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-25316854

RESUMO

BACKGROUND: Inactivated poliovirus vaccine (IPV) is rarely used in tropical developing countries. To generate additional scientific information, especially on the possible emergence of vaccine-derived polioviruses (VDPVs) in an IPV-only environment, we initiated an IPV introduction project in Yogyakarta, an Indonesian province. In this report, we present the coverage, immunity, and VDPV surveillance results. METHODS: In Yogyakarta, we established environmental surveillance starting in 2004; and conducted routine immunization coverage and seroprevalence surveys before and after a September 2007 switch from oral poliovirus vaccine (OPV) to IPV, using standard coverage and serosurvey methods. Rates and types of polioviruses found in sewage samples were analyzed, and all poliovirus isolates after the switch were sequenced. RESULTS: Vaccination coverage (>95%) and immunity (approximately 100%) did not change substantially before and after the IPV switch. No VDPVs were detected. Before the switch, 58% of environmental samples contained Sabin poliovirus; starting 6 weeks after the switch, Sabin polioviruses were rarely isolated, and if they were, genetic sequencing suggested recent introductions. CONCLUSIONS: This project demonstrated that under almost ideal conditions (good hygiene, maintenance of universally high IPV coverage, and corresponding high immunity against polioviruses), no emergence and circulation of VDPV could be detected in a tropical developing country setting.


Assuntos
Monitoramento Ambiental , Poliomielite/prevenção & controle , Vacina Antipólio de Vírus Inativado/administração & dosagem , Vacina Antipólio de Vírus Inativado/imunologia , Poliovirus/isolamento & purificação , Esgotos/virologia , Vacinação/métodos , Animais , Anticorpos Antivirais/sangue , Pré-Escolar , Feminino , Humanos , Indonésia , Lactente , Masculino , Poliovirus/classificação , Poliovirus/genética , Vacinação/estatística & dados numéricos
5.
J Virol ; 87(23): 13076-80, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24049178

RESUMO

Highly divergent vaccine-derived polioviruses (VDPVs) have been isolated from sewage in Tallinn, Estonia, since 2002. Sequence analysis of VDPVs of serotypes 2 and 3 showed that they shared common noncapsid region recombination sites, indicating origination from a single trivalent oral polio vaccine dose, estimated to have been given between 1986 and 1998. The sewage isolates closely resemble VDPVs chronically excreted by persons with common variable immunodeficiency, but no chronic excretors have yet been identified in Estonia.


Assuntos
Evolução Molecular , Poliomielite/virologia , Vacinas contra Poliovirus/genética , Poliovirus/genética , Esgotos/virologia , Sequência de Bases , Proteínas do Capsídeo/química , Proteínas do Capsídeo/genética , Estônia , Humanos , Dados de Sequência Molecular , Filogenia , Poliomielite/prevenção & controle , Poliovirus/química , Poliovirus/classificação , Poliovirus/isolamento & purificação , Vacinas contra Poliovirus/química , Vacinas contra Poliovirus/classificação , Vacinas contra Poliovirus/isolamento & purificação , Recombinação Genética , Alinhamento de Sequência
6.
J Virol ; 86(10): 5959-62, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22398295

RESUMO

At 37°C, the structure of poliovirus is dynamic, and internal polypeptides VP4 and N terminus of VP1 (residues 1 to 53) externalize reversibly. An Fab fragment of a monospecific antibody, which binds to residues 39 to 55 of VP1, was utilized to locate the N termini of VP1 in native (160S) particles in this "breathing" state. Fab and virus were mixed and imaged via cryogenic electron microscopy. The resulting reconstruction showed the capsid expands similarly to the irreversibly altered cell entry intermediate (135S) particle, but the N terminus of VP1 is located near the 2-fold axes, instead of the "propeller tip" as in 135S particles.


Assuntos
Proteínas do Capsídeo/química , Fragmentos Fab das Imunoglobulinas/análise , Poliovirus/química , Capsídeo/química , Microscopia Crioeletrônica , Humanos , Modelos Moleculares , Poliomielite/virologia , Poliovirus/metabolismo
7.
J Virol ; 86(20): 11163-70, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22855495

RESUMO

Coxsackievirus A9 (CAV9), a member of the Picornaviridae family, uses an RGD motif in the VP1 capsid protein to bind to integrin αvß6 during cell entry. Here we report that two CAV9 isolates can bind to the heparan sulfate/heparin class of proteoglycans (HSPG). Sequence analysis identified an arginine (R) at position 132 in VP1 in these two isolates, rather than a threonine (T) as seen in the nonbinding strains tested. We introduced a T132R substitution into the HSPG-nonbinding strain Griggs and recovered infectious virus capable of binding to immobilized heparin, unlike the parental Griggs strain. The known CAV9 structure was used to identify the location of VP1 position 132, 5 copies of which were found to cluster around the 5-fold axis of symmetry, presumably producing a region of positive charge which can interact with the negatively charged HSPG. Analysis of several enteroviruses of the same species as CAV9, Human enterovirus B (HEV-B), identified examples from 5 types in which blocking of infection by heparin was coincident with an arginine (or another basic amino acid, lysine) at a position corresponding to 132 in VP1 in CAV9. Together, these data show that membrane-associated HSPG can serve as a (co)receptor for some CAV9 and other HEV-B strains and identify symmetry-related clustering of positive charges as one mechanism by which HSPG binding can be achieved. This is a potentially powerful mechanism by which a single amino acid change could generate novel receptor binding capabilities, underscoring the plasticity of host-cell interactions in enteroviruses.


Assuntos
Proteínas do Capsídeo/metabolismo , Enterovirus Humano B/genética , Enterovirus Humano B/metabolismo , Proteoglicanas de Heparan Sulfato/metabolismo , Proteínas Virais/metabolismo , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Antígenos de Neoplasias/metabolismo , Sítios de Ligação , Linhagem Celular , Chlorocebus aethiops , Infecções por Enterovirus/metabolismo , Integrinas/metabolismo , Dados de Sequência Molecular , Ligação Proteica , Receptores Virais/metabolismo , Análise de Sequência de RNA , Eletricidade Estática
8.
PLoS Pathog ; 7(9): e1002267, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21977009

RESUMO

The rise in type 1 diabetes (T1D) incidence in recent decades is probably related to modifications in environmental factors. Viruses are among the putative environmental triggers of T1D. The mechanisms regulating beta cell responses to viruses, however, remain to be defined. We have presently clarified the signaling pathways leading to beta cell apoptosis following exposure to the viral mimetic double-stranded RNA (dsRNA) and a diabetogenic enterovirus (Coxsackievirus B5). Internal dsRNA induces cell death via the intrinsic mitochondrial pathway. In this process, activation of the dsRNA-dependent protein kinase (PKR) promotes eIF2α phosphorylation and protein synthesis inhibition, leading to downregulation of the antiapoptotic Bcl-2 protein myeloid cell leukemia sequence 1 (Mcl-1). Mcl-1 decrease results in the release of the BH3-only protein Bim, which activates the mitochondrial pathway of apoptosis. Indeed, Bim knockdown prevented both dsRNA- and Coxsackievirus B5-induced beta cell death, and counteracted the proapoptotic effects of Mcl-1 silencing. These observations indicate that the balance between Mcl-1 and Bim is a key factor regulating beta cell survival during diabetogenic viral infections.


Assuntos
Proteínas Reguladoras de Apoptose/metabolismo , Apoptose , Infecções por Coxsackievirus/metabolismo , Enterovirus Humano B/metabolismo , Células Secretoras de Insulina/metabolismo , Proteínas de Membrana/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , RNA de Cadeia Dupla/metabolismo , RNA Viral/metabolismo , Animais , Proteína 11 Semelhante a Bcl-2 , Linhagem Celular , Sobrevivência Celular , Infecções por Coxsackievirus/patologia , Diabetes Mellitus Tipo 1/etiologia , Diabetes Mellitus Tipo 1/metabolismo , Diabetes Mellitus Tipo 1/patologia , Diabetes Mellitus Tipo 1/virologia , Fator de Iniciação 2 em Eucariotos/metabolismo , Humanos , Células Secretoras de Insulina/patologia , Células Secretoras de Insulina/virologia , Masculino , Mitocôndrias/metabolismo , Proteína de Sequência 1 de Leucemia de Células Mieloides , Fosforilação , Ratos , Ratos Wistar
9.
Appl Environ Microbiol ; 79(12): 3534-43, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23524678

RESUMO

Bacillus cereus, aseptically isolated from potato tubers, were screened for cereulide production and for toxicity on human and other mammalian cells. The cereulide-producing isolates grew slowly, the colonies remained small (~1 mm), tested negative for starch hydrolysis, and varied in productivity from 1 to 100 ng of cereulide mg (wet weight)(-1) (~0.01 to 1 ng per 10(5) CFU). By DNA-fingerprint analysis, the isolates matched B. cereus F5881/94, connected to human food-borne illness, but were distinct from cereulide-producing endophytes of spruce tree (Picea abies). Exposure to cell extracts (1 to 10 µg of bacterial biomass ml(-1)) and to purified cereulide (0.4 to 7 ng ml(-1)) from the potato isolates caused mitochondrial depolarization (loss of ΔΨm) in human peripheral blood mononuclear cells (PBMC) and keratinocytes (HaCaT), porcine spermatozoa and kidney tubular epithelial cells (PK-15), murine fibroblasts (L-929), and pancreatic insulin-producing cells (MIN-6). Cereulide (10 to 20 ng ml(-1)) exposed pancreatic islets (MIN-6) disintegrated into small pyknotic cells, followed by necrotic death. Necrotic death in other test cells was observed only after a 2-log-higher exposure. Exposure to 30 to 60 ng of cereulide ml(-1) induced K(+) translocation in intact, live PBMC, keratinocytes, and sperm cells within seconds of exposure, depleting 2 to 10% of the cellular K(+) stores within 10 min. The ability of cereulide to transfer K(+) ions across biological membranes may benefit the producer bacterium in K(+)-deficient environments such as extracellular spaces inside plant tissue but is a pathogenic trait when in contact with mammalian cells.


Assuntos
Bacillus cereus/química , Depsipeptídeos/metabolismo , Eméticos/metabolismo , Solanum tuberosum/microbiologia , Animais , Depsipeptídeos/toxicidade , Eméticos/toxicidade , Células Epiteliais/efeitos dos fármacos , Humanos , Células Secretoras de Insulina/efeitos dos fármacos , Queratinócitos/efeitos dos fármacos , Túbulos Renais/citologia , Leucócitos Mononucleares/efeitos dos fármacos , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Solanum tuberosum/crescimento & desenvolvimento , Espermatozoides/efeitos dos fármacos , Suínos , Fatores de Tempo , Testes de Toxicidade
10.
J Med Virol ; 85(8): 1466-72, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23765783

RESUMO

The significance of human rhinoviruses (HRV) as prevailing respiratory pathogens has sharpened during the recent years followed by implementation of molecular methods in detection. Rhinoviruses are detected exceedingly in hospitalized cases of respiratory infection with varying severity, in addition to being frequent in cases of common cold. The aim of this study was to evaluate occurrence of HRV in a prospective study material. The prospective INDIS material comprises nasopharyngeal (N=429) and fecal (N=425) specimens from children under 11 years of age collected during any clinical infection. Validated real-time RT-PCR assays were applied for the detection of HRV. HRV were detected numerously not only in the nasopharyngeal specimens, but a myriad also in fecal specimens, 236 (55.0%) and 149 (35.1%), respectively, fecal findings actually beyond anticipation. A total of 13 of HRV-positive fecal specimens were selected for genetic typing in the VP4/VP2 coding region. HRV-A strains were detected in seven specimens: HRV-A9, -A10, -A24, -A49, -A56 and -A82. HRV-B-strains were detected three times: HRV-B42 and -B79, and HRV-C twice: HRV-C12 and HRV-Cpat4. HRV-B42 also showed cytopathic effect in cell culture, confirmed by real-time RT-PCR and VP4/VP2 sequencing, suggesting presence of viable HRV in fecal specimens.


Assuntos
Fezes/virologia , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/virologia , Rhinovirus/isolamento & purificação , Criança , Pré-Escolar , Feminino , Genótipo , Humanos , Lactente , Recém-Nascido , Masculino , Dados de Sequência Molecular , Nasofaringe/virologia , Prevalência , Estudos Prospectivos , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rhinovirus/genética , Análise de Sequência de DNA
11.
J Med Virol ; 85(9): 1632-8, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23794458

RESUMO

Limited data are available on the effects of probiotics on the nasopharyngeal presence of respiratory viruses in children attending day care. In this substudy of a randomized, double-blinded, placebo-controlled 28-week intervention study, nasopharyngeal swab samples were collected, on visits to a physician due to symptoms of infection, from children receiving control milk (N = 97) and children receiving the same milk supplemented with probiotic Lactobacillus rhamnosus GG (N = 97). The presence of 14 respiratory viruses was assessed by PCR methods, and viral findings were compared with symptom prevalences in the intervention groups. Rhinovirus was identified in 28.6% of 315 swab samples, followed by respiratory syncytial virus (12.4%), parainfluenza virus 1 (12.1%), enterovirus (8.9%), influenza A(H1N1)pdm09 (7.9%), human bocavirus 1 (3.8%), parainfluenza virus 2 (3.2%), adenovirus (2.9%), and influenza A(H3N2) (0.6%). The children in the probiotic group had less days with respiratory symptoms per month than the children in the control group (6.48 [95% CI 6.28-6.68] vs. 7.19 [95% CI 6.98-7.41], P < 0.001). Probiotic intervention did not reduce significantly the occurrence of the examined respiratory viruses, or have an effect on the number of respiratory symptoms observed at the time of a viral finding. Rhinovirus, respiratory syncytial virus, and parainfluenza virus 1 were the most common respiratory viruses in symptomatic children. Children receiving Lactobacillus rhamnosus GG had fewer days with respiratory symptoms than children in the control group, although probiotic intervention was not effective in reducing the amount of viral findings or the respiratory symptoms associated with viral findings.


Assuntos
Lacticaseibacillus rhamnosus/imunologia , Nasofaringe/virologia , Probióticos/administração & dosagem , Viroses/prevenção & controle , Vírus/isolamento & purificação , Criança , Creches , Pré-Escolar , Método Duplo-Cego , Feminino , Humanos , Masculino , Placebos/administração & dosagem , Prevalência , Resultado do Tratamento , Viroses/epidemiologia , Viroses/patologia , Viroses/virologia , Vírus/classificação
12.
J Med Virol ; 85(9): 1678-84, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23794481

RESUMO

The frequencies of early childhood infections were studied in healthy children with increased genetic risk for type 1 diabetes participating in the ongoing prospective high intensive infection follow-up Study, INDIS, started in 2009 in Turku, Finland. Here the results obtained from 160 stool to 160 nasal swab specimens collected in parallel at times of infectious symptoms in 2009-2010 from 45 children at the age of 24 months or younger are reported. The specimens were analyzed for enteric (human enterovirus, norovirus, rotavirus, sapovirus, astrovirus) and respiratory RNA viruses (human enterovirus and rhinovirus) common in early childhood, respectively, using highly validated virus-specific real-time PCR methods. According to the results 96% of the children had at least one virus infection during the study period and one or several viral agents were detected in 76% of sample sets. The most prevalent viral agents were human rhinovirus, enterovirus, parechovirus, and norovirus (genotype GII) with positive specimens 57.5%, 28.8%, 19.4%, and 6.9%, respectively. Other intestinal viruses were found in less than 2% of stool specimens. Single infections covered 40.0% of the specimens while multiple infections with two or more infectious agents were detected in 36.3% of specimens and altogether 11 combinations of viruses were included in the mixed infections. Although human enterovirus is known to be a frequent finding in stool specimens, especially during early childhood, it was found in this study more frequently in nasal swab specimens. Whether this is true, more general, in countries with the high hygiene level remains to be shown.


Assuntos
Viroses/epidemiologia , Viroses/virologia , Vírus/classificação , Vírus/isolamento & purificação , Doenças Assintomáticas , Pré-Escolar , Estudos de Coortes , Coinfecção/epidemiologia , Coinfecção/virologia , Fezes/virologia , Feminino , Finlândia/epidemiologia , Humanos , Lactente , Recém-Nascido , Masculino , Mucosa Nasal/virologia , Reação em Cadeia da Polimerase , Prevalência , Estudos Prospectivos
13.
Diabetes Metab Res Rev ; 29(7): 557-67, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23674484

RESUMO

BACKGROUND: The vast array and quantity of longitudinal samples collected in The Environmental Determinants of Diabetes in the Young study present a series of challenges in terms of quality control procedures and data validity. To address this, pilot studies have been conducted to standardize and enhance both biospecimen collection and sample obtainment in terms of autoantibody collection, stool sample preservation, RNA, biomarker stability, metabolic biomarkers and T-cell viability. RESEARCH DESIGN AND METHODS: The Environmental Determinants of Diabetes in the Young is a multicentre, international prospective study (n = 8677) designed to identify environmental triggers of type 1 diabetes (T1D) in genetically at-risk children from ages 3 months until 15 years. The study is conducted through six primary clinical centres located in four countries. RESULTS: As of May 2012, over three million biological samples and 250 million total data points have been collected, which will be analysed to assess autoimmunity status, presence of inflammatory biomarkers, genetic factors, exposure to infectious agents, dietary biomarkers and other potentially important environmental exposures in relation to autoimmunity and progression to T1D. CONCLUSIONS: Detailed procedures were utilized to standardize both data harmonization and management when handling a large quantity of longitudinal samples obtained from multiple locations. In addition, a description of the available specimens is provided that serve as an invaluable repository for the elucidation of determinants in T1D focusing on autoantibody concordance and harmonization, transglutaminase autoantibody, inflammatory biomarkers (T-cells), genetic proficiency testing, RNA lab internal quality control testing, infectious agents (monitoring cross-contamination, virus preservation and nasal swab collection validity) and HbA1c testing.


Assuntos
Bancos de Espécimes Biológicos/organização & administração , Bancos de Espécimes Biológicos/normas , Diabetes Mellitus Tipo 1/patologia , Manejo de Espécimes/métodos , Manejo de Espécimes/normas , Adolescente , Autoanticorpos/sangue , Criança , Pré-Escolar , Diabetes Mellitus Tipo 1/genética , Fezes/virologia , Humanos , Lactente , Recém-Nascido , Estudos Longitudinais , Controle de Qualidade , RNA Mensageiro/análise
15.
Appl Environ Microbiol ; 78(15): 5406-9, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22582070

RESUMO

Systematic environmental surveillance for poliovirus circulation has been conducted in Egypt since 2000. The surveillance has revealed three independent importations of wild-type poliovirus. In addition, several vaccine-derived polioviruses have been detected in various locations in Egypt. In addition to acute flaccid paralysis (AFP) surveillance, environmental surveillance can be used to monitor the wild poliovirus and vaccine-derived poliovirus circulation in populations in support of polio eradication initiatives.


Assuntos
Microbiologia Ambiental , Paralisia/epidemiologia , Poliovirus/genética , Vigilância em Saúde Pública/métodos , Esgotos/virologia , Sequência de Bases , Egito/epidemiologia , Humanos , Dados de Sequência Molecular , Paralisia/virologia , Filogenia , Poliomielite/epidemiologia , Dinâmica Populacional , Análise de Sequência de DNA
16.
J Med Virol ; 84(9): 1497-500, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22825830

RESUMO

Saffold viruses (SAFV) have been discovered recently and they are classified into Theilovirus species in genus Cardiovirus in the Picornaviridae family. SAFV, especially those belonging to the genotype 2, have been difficult to propagate in laboratory cell lines. This study describes the successful isolation of an efficiently growing SAFV-2 strain directly from a stool specimen by standard virological methods. The availability of SAFV isolates that can be propagated to high titers is crucial to the future studies on pathogenesis and epidemiology of these novel human viruses.


Assuntos
Infecções por Cardiovirus/veterinária , Chlorocebus aethiops/virologia , Doenças dos Macacos/virologia , Theilovirus/isolamento & purificação , Animais , Infecções por Cardiovirus/virologia , Fezes/virologia , Humanos , Rim/citologia , Filogeografia , Análise de Sequência de DNA , Theilovirus/genética , Células Vero , Proteínas Virais/genética
17.
J Med Virol ; 84(2): 345-7, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-22170557

RESUMO

The causes of exacerbations of inflammatory bowel disease (IBD) are unknown. The presence of RNA of an enterovirus, norovirus GI, norovirus GII, rotavirus, astrovirus, and sapovirus was sought in stool samples of 50 children (median age 12.9 years) undergoing gastrointestinal endoscopies for IBD or its exclusion (Crohn's disease n = 18, ulcerative colitis n = 13, indeterminate colitis n = 2, non-IBD n = 17). Viral RNA was found in three fecal samples (norovirus GII n = 2, sapovirus n = 1), all in children without IBD. Therefore, enteral viruses may play only a minor role in IBD.


Assuntos
Colite Ulcerativa/epidemiologia , Colite Ulcerativa/virologia , Doença de Crohn/virologia , Fezes/virologia , RNA Viral/isolamento & purificação , Adolescente , Astroviridae/genética , Criança , Pré-Escolar , Doença de Crohn/epidemiologia , Enterovirus/genética , Feminino , Humanos , Masculino , Norovirus/genética , Rotavirus/genética , Sapovirus/genética
18.
J Med Virol ; 84(7): 1080-3, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22585725

RESUMO

Human enteroviruses are the most common cause of viral meningitis. Viral-bacterial interaction may affect the clinical course and outcome of bacterial meningitis. In Africa, viruses might be responsible for 14-25% of all meningitis cases. However, only few studies from Africa have reported detection of viruses in the cerebrospinal fluid (CSF) or mixed viral-bacterial infections of the central nervous system (CNS). The aim of the present study was to investigate the presence of picornaviruses in the CSF of children suffering from meningitis in Luanda, Angola. The study included 142 consecutive children enrolled in a prospective study of bacterial meningitis in Luanda between 2005 and 2006, from whom a CSF sample was available. CSF samples were obtained at hospital admission, stored in a deep-freeze, and transported to Finland for testing by real-time PCR for picornaviruses. Enteroviruses were detected in 4 (3%) of 142 children with presumed bacterial meningitis. A 5-month-old girl with rhinovirus and Haemophilus influenzae meningitis recovered uneventfully. An 8-year-old girl with human enterovirus and pneumococcal meningitis developed no sequelae. A 2-month-old girl with human enterovirus and malaria recovered quickly. A 7-month-old girl with human enterovirus was treated for presumed tuberculous meningitis and survived with severe sequelae. Mixed infections of the CNS with picornaviruses and bacteria are rare. Detection of an enterovirus does not affect the clinical picture and outcome of bacterial meningitis.


Assuntos
Líquido Cefalorraquidiano/virologia , Meningite Viral/epidemiologia , Meningite Viral/virologia , Infecções por Picornaviridae/epidemiologia , Infecções por Picornaviridae/virologia , Picornaviridae/isolamento & purificação , Angola/epidemiologia , Criança , Coinfecção/epidemiologia , Coinfecção/virologia , Feminino , Humanos , Lactente , Estudos Prospectivos
19.
J Med Virol ; 84(3): 543-7, 2012 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-22246844

RESUMO

Ethanol-containing hand rubs are used frequently as a substitute for hand washing with water and soap. However, not all viruses are inactivated by a short term rubbing with alcohol. The capacity of a single round of instructed and controlled hand cleaning with water and soap or ethanol-containing hand rub, respectively, was tested for removal of human rhinovirus administered onto the skin of healthy volunteers on the back of the hands. Hand washing with soap and water appeared to be much more efficient for removing rhinoviruses from skin than rubbing hands with an ethanol-containing disinfectant. After washing with soap and water the virus was detected in 3/9 (33.3%) test persons from the left hand and 1/9 (11.1%) cases from the right hand, whereas the virus was detected invariably by real-time RT-PCR from both hands after cleaning with alcohol hand rub (P-value <0.01). Both substances evaluated clinically were also tested in vitro for virucidal efficacy against Human rhinovirus2 (HRV2) using a standardized assay. Both tested substances were poor within the contact time used in the hand-cleaning test. In conclusion, thorough and conventional hand washing with water and soap can clean efficiently hands contaminated with the virus responsible for an extensive share of common cold episodes.


Assuntos
Anti-Infecciosos Locais/farmacologia , Desinfetantes/farmacologia , Etanol/farmacologia , Desinfecção das Mãos/métodos , Rhinovirus/efeitos dos fármacos , Sabões/farmacologia , Humanos , RNA Viral , Reação em Cadeia da Polimerase em Tempo Real , Rhinovirus/genética , Rhinovirus/isolamento & purificação , Higiene da Pele/métodos , Inativação de Vírus/efeitos dos fármacos
20.
J Med Virol ; 84(6): 923-30, 2012 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-22499016

RESUMO

Human enteroviruses (HEV) are common, especially in childhood and during the enterovirus season, causing mainly asymptomatic infections but also mild and severe illnesses. Numerous studies have shown the association between HEV infections and type 1 diabetes. Here, the prevalence of HEV infections was studied in healthy Swedish children with increased HLA-associated risk for type 1 diabetes participating in the PRODIA pilot study in which children were randomized to receive probiotics or placebo during the first 6 months of life. Stool specimens collected from 197 children in every 3 months from the age of 3 to 24 months were screened for HEV using traditional viral culturing method and identified with reverse transcriptase polymerase chain reaction (RT-PCR) and sequencing of the partial VP1 coding part of the viral genome. Altogether 4.8% (52/1,094) of the specimens were HEV-positive and 22.3% (44/197) of the children excreted HEV during the follow-up. HEV-A and HEV-B were present in 2.1 and 2.7% of the specimens, respectively. HEV-C and HEV-D viruses were not detected. In total, 17 different HEV serotypes were detected and the most common findings were CV-A9 (13.5%), CV-A16 (11.5%), and CV-A2 (9.6%). The majority of the infections (92.3%) were during the enterovirus season extending from July to December. Probiotic treatment did not affect significantly the risk of HEV infections during the 2-year follow-up although a trend for transient decrease for HEV positivity (HEV-A and/or HEV-B) by the age of 12 months was observed in children who received probiotics [OR 0.40; 95% confidence interval 0.15 to 1.08; P-value 0.071, generalized estimating (GEE) analysis]. According to the results, HEV-A findings were nearly as common as HEV-B findings among the healthy children participating in this study. Also it was shown that serotypes belonging to HEV-A species can be detected by means of viral culturing.


Assuntos
Infecções por Enterovirus/epidemiologia , Enterovirus/isolamento & purificação , Pré-Escolar , Enterovirus/genética , Fezes/virologia , Humanos , Lactente , Recém-Nascido , Dados de Sequência Molecular , Projetos Piloto , Placebos/administração & dosagem , Prevalência , Probióticos/administração & dosagem , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA , Suécia/epidemiologia , Cultura de Vírus
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