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1.
Hum Mol Genet ; 25(19): 4227-4243, 2016 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-27506979

RESUMO

Mutations that cause increased and/or inappropriate activation of FGFR3 are responsible for a collection of short-limbed chondrodysplasias. These mutations can alter receptor trafficking and enhance receptor stability, leading to increased receptor accumulation and activity. Here, we show that wildtype and mutant activated forms of FGFR3 increase expression of the cytoplasmic deacetylase HDAC6 (Histone Deacetylase 6) and that FGFR3 accumulation is compromised in cells lacking HDAC6 or following treatment of fibroblasts or chondrocytes with small molecule inhibitors of HDAC6. The reduced accumulation of FGFR3 was linked to increased FGFR3 degradation that occurred through a lysosome-dependent mechanism. Using a mouse model of Thanatophoric Dysplasia Type II (TDII) we show that both HDAC6 deletion and treatment with the small molecule HDAC6 inhibitor tubacin reduced FGFR3 accumulation in the growth plate and improved endochondral bone growth. Defective endochondral growth in TDII is associated with reduced proliferation and poor hypertrophic differentiation and the improved bone growth was associated with increased chondrocyte proliferation and expansion of the differentiation compartment within the growth plate. These findings further define the mechanisms that control FGFR3 accumulation and contribute to skeletal pathology caused by mutations in FGFR3.


Assuntos
Acondroplasia/genética , Histona Desacetilases/genética , Receptor Tipo 3 de Fator de Crescimento de Fibroblastos/metabolismo , Crânio/anormalidades , Displasia Tanatofórica/genética , Acondroplasia/tratamento farmacológico , Acondroplasia/metabolismo , Acondroplasia/patologia , Anilidas/administração & dosagem , Animais , Desenvolvimento Ósseo/genética , Diferenciação Celular/genética , Proliferação de Células/genética , Condrócitos/efeitos dos fármacos , Condrócitos/patologia , Modelos Animais de Doenças , Inibidores Enzimáticos/administração & dosagem , Fibroblastos/efeitos dos fármacos , Fibroblastos/patologia , Desacetilase 6 de Histona , Inibidores de Histona Desacetilases/administração & dosagem , Humanos , Ácidos Hidroxâmicos/administração & dosagem , Camundongos , Mutação , Receptor Tipo 3 de Fator de Crescimento de Fibroblastos/genética , Crânio/metabolismo , Crânio/patologia , Displasia Tanatofórica/metabolismo , Displasia Tanatofórica/patologia
3.
J Chem Neuroanat ; 89: 11-20, 2018 04.
Artigo em Inglês | MEDLINE | ID: mdl-29481900

RESUMO

Tics and compulsions in comorbid Tourette's syndrome (TS) and obsessive-compulsive disorder (OCD) are associated with chronic hyperactivity of parallel cortico/amygdalo-striato-thalamo-cortical (CSTC) loop circuits. Comorbid TS- & OCD-like behaviors have likewise been observed in D1CT-7 mice, in which an artificial neuropotentiating transgene encoding the cAMP-elevating intracellular subunit of cholera toxin (CT) is chronically expressed selectively in somatosensory cortical & amygdalar dopamine (DA) D1 receptor-expressing neurons that activate cortico/amygdalo-striatal glutamate (GLU) output. We've now examined in D1CT-7 mice whether the chronic GLU output from their potentiated cortical/limbic CSTC subcircuit afferents associated with TS- & OCD-like behaviors elicits desensitizing neurochemical changes in the striatum (STR). Microdialysis-capillary electrophoresis and in situ hybridization reveal that the mice's chronic GLU-excited STR exhibits pharmacodynamic changes in three independently GLU-regulated measures of output neuron activation, co-excitation, and desensitization, signifying hyperactive striatal CSTC output and compensatory striatal glial and neuronal desensitization: 1) Striatal GABA, an output neurotransmitter induced by afferent GLU, is increased. 2) Striatal d-serine, a glial excitatory co-transmitter inhibited by afferent GLU, is decreased. 3) Striatal Period1 (Per1), which plays a non-circadian role in the STR as a GLU + DA D1- (cAMP-) dependent repressor thought to feedback-inhibit GLU + DA- triggered ultradian urges and motions, is transcriptionally abolished. These data imply that chronic cortical/limbic GLU excitation of the STR desensitizes its co-excitatory d-serine & DA inputs while freezing its GABA output in an active state to mediate chronic tics and compulsions - possibly in part by abolishing striatal Per1-dependent ultradian extinction of urges and motions.


Assuntos
Biomarcadores/análise , Encéfalo/fisiopatologia , Transtorno Obsessivo-Compulsivo/fisiopatologia , Síndrome de Tourette/fisiopatologia , Animais , Encéfalo/metabolismo , Modelos Animais de Doenças , Glutamina/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Transgênicos , Transtorno Obsessivo-Compulsivo/metabolismo , Síndrome de Tourette/metabolismo
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