RESUMO
To examine the action mechanism that mediates the anti-fertility effect of Costus speciosus extract, research was conducted on male Sprague-Dawley rats. Costus extract was given to male rats for 14 days at various doses, namely 275, 550 and 1,100 mg kg-1 day-1 in 0.5% sodium CMC. The results showed that Costus extract with doses ranging from 275 to 1,100 mg kg-1 day-1 was able to inhibit pregnancy among female rats by 10-70%. No obstacles in terms of sexual behavior were identified among male rats. The anti-fertility effect of Costus extract kicked in without involving a decreased level of male reproductive hormones.
Assuntos
Anticoncepcionais Masculinos/farmacologia , Costus/química , Extratos Vegetais/farmacologia , Comportamento Sexual Animal/efeitos dos fármacos , Animais , Anticoncepcionais Masculinos/química , Relação Dose-Resposta a Droga , Feminino , Hormônio Foliculoestimulante/metabolismo , Hormônio Luteinizante/metabolismo , Masculino , Extratos Vegetais/química , Folhas de Planta/química , Ratos , Testículo/metabolismo , Testosterona/metabolismoRESUMO
OBJECTIVE: Gonadotropin-inhibitory hormone (GnIH)-3 is a neuropeptide that plays a major role in the regulation of reproduction and feeding in mammals. MATERIALS AND METHODS: We measured endocrine and behavioural parameters of reproduction in sheep, and sexual behaviour in sheep, mice and cynomolgus monkeys. In addition, GnIH gene expression (in situ hybridization) was examined in ewes, and effects of GnIH-3 on food intake and energy expenditure were measured in various species. GnIH-3 was infused (i.v.) into ewes after an i.m. injection of estradiol benzoate to determine whether the peptide blocks the surge in luteinizing hormone (LH) secretion. RESULTS: GnIH gene expression was reduced in the preovulatory period in ewes. Infusion (i.v.) of GnIH-3 blocked the estrogen-induced LH surge (in ewes). Intracerebroventricular infusion had no effect on female or male sexual behaviour in each of the three species, but increased food intake. There were no effects on energy expenditure in sheep or rats. GnIH increased fos protein (immunohistochemistry) was seen in orexigenic neurons (in sheep and rats), but also in anorexigenic neurons (in sheep). CONCLUSIONS: GnIH-3 reduces reproductive hormone levels and increases food intake in mammals without reducing energy expenditure. There is minimal effect on reproductive behaviour. The dual effect on reproduction and feeding suggests that GnIH-3 provides a molecular switch between these two functions. Blockade of the positive feedback effect of estrogen with parenteral infusion indicates that this peptide may have utility as a blocker of reproductive function in mammals.
Assuntos
Comportamento Alimentar/fisiologia , Glicoproteínas/fisiologia , Hormônios Hipotalâmicos/fisiologia , Reprodução , Animais , Avaliação Pré-Clínica de Medicamentos , Ingestão de Alimentos/efeitos dos fármacos , Ingestão de Alimentos/genética , Ingestão de Alimentos/fisiologia , Comportamento Alimentar/efeitos dos fármacos , Feminino , Genes de Troca/fisiologia , Glicoproteínas/genética , Glicoproteínas/farmacologia , Hormônios Hipotalâmicos/genética , Hormônios Hipotalâmicos/farmacologia , Macaca fascicularis , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neuropeptídeos/genética , Neuropeptídeos/farmacologia , Neuropeptídeos/fisiologia , Ratos , Reprodução/efeitos dos fármacos , Reprodução/genética , Comportamento Sexual Animal/efeitos dos fármacos , Comportamento Sexual Animal/fisiologia , OvinosRESUMO
Melioidosis, an infectious disease caused by Burkholderia pseudomallei, has recently gained importance as an emerging infectious disease in Indonesia. Reports of this infection in Indonesia are limited, although cases have been reported in Makassar, South Sulawesi. We report a case of cutaneous melioidosis caused by pan-drug-resistant, moderate biofilm-producer strain of B. pseudomallei in a diabetic patient. To the best of our knowledge, this is the first case of melioidosis caused by multidrug resistant and biofilm-former strain of B. pseudomallei being reported from Yogyakarta Province, Indonesia. The patient was successfully treated with abscess drainage and debridement, including total contact casting and no antibiotic treatment.
RESUMO
The present study aimed to examine the immunomodulatory effect of ethanolic extract of Typhonium flagelliforme (Lodd) Blume in cyclophosphamide-treated rats. The immunomodulatory effects were determined by lymphocytes proliferation, phagocytic activity of macrophages, plasma cytokines of tumor necrosis factor-α, interleukin-1α, interleukin-10 levels, and killer T cells (CD8+ T cells) counts. The results showed that the administration of ethanolic extract of T flagelliforme reduced immunosupessive effect on lymphocyte proliferation, increase the number and phagocytic activity of macrophages in cyclophosphamide-treated rats. Moreover, the ethanolic extract of T flagelliforme also significantly (P < .05) improved the immune system activities especially the proliferation of CD8+T cells and reduced the suppressive effects on cytokines such as tumor necrosis factor-α and interleukin-1α. In conclusion, the ethanolic extract of T flagelliforme has immunomodulatory properties in cyclophosphamide-treated rats. The results suggest that T flagelliforme can reduce immunosuppresive effect caused by a chemotherapeutic agent.