RESUMO
Phospholipase A2 (Pla2) is required for memory retrieval, and its inhibition in the hippocampus has been reported to impair memory acquisition in rats. Moreover, cognitive decline and memory deficits showed to be reduced in animal models after lithium treatment, prompting us to evaluate possible links between Pla2, lithium and memory. Here, we evaluated the possible modulation of Pla2 activity by a long-term treatment of rats with low doses of lithium and its impact in memory. Wistar rats were trained for the inhibitory avoidance task, treated with lithium for 100 days and tested for perdurability of long-term memory. Hippocampal samples were used for quantifying the expression of 19 brain-expressed Pla2 genes and for evaluating the enzymatic activity of Pla2 using group-specific radio-enzymatic assays. Our data pointed to a significant perdurability of long-term memory, which correlated with increased transcriptional and enzymatic activities of certain members of the Pla2 family (iPla2 and sPla2) after the chronic lithium treatment. Our data suggest new possible targets of lithium, add more information on its pharmacological activity and reinforce the possible use of low doses of lithium for the treatment of neurodegenerative conditions such as the Alzheimer's disease.
Assuntos
Comportamento Animal/efeitos dos fármacos , Hipocampo/enzimologia , Compostos de Lítio/farmacologia , Memória de Longo Prazo/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Fosfolipases A2/efeitos dos fármacos , Doença de Alzheimer/tratamento farmacológico , Animais , Hipocampo/efeitos dos fármacos , Compostos de Lítio/administração & dosagem , Masculino , Fármacos Neuroprotetores/administração & dosagem , Fosfolipases A2/genética , Ratos , Ratos WistarRESUMO
Rare earth elements (REEs) have been intentionally used in Chinese agriculture since the 1980s to improve crop yields. Around the world, REEs are also involuntarily applied to soils through phosphate fertilizers. These elements are known to alleviate damage in plants under abiotic stresses, yet there is no information on how these elements act in the physiology of plants. The REE mode of action falls within the scope of the hormesis effect, with low-dose stimulation and high-dose adverse reactions. This study aimed to verify how REEs affect rice plants' physiology to test the threshold dose at which REEs could act as biostimulants in these plants. In experiment 1, 0.411 kg ha-1 (foliar application) of a mixture of REE (containing 41.38% Ce, 23.95% La, 13.58% Pr, and 4.32% Nd) was applied, as well as two products containing 41.38% Ce and 23.95% La separately. The characteristics of chlorophyll a fluorescence, gas exchanges, SPAD index, and biomass (pot conditions) were evaluated. For experiment 2, increasing rates of the REE mix (0, 0.1, 0.225, 0.5, and 1 kg ha-1) (field conditions) were used to study their effect on rice grain yield and nutrient concentration of rice leaves. Adding REEs to plants increased biomass production (23% with Ce, 31% with La, and 63% with REE Mix application) due to improved photosynthetic rate (8% with Ce, 15% with La, and 27% with REE mix), favored by the higher electronic flow (photosynthetic electron transport chain) (increase of 17%) and by the higher Fv/Fm (increase of 14%) and quantum yield of photosystem II (increase of 20% with Ce and La, and 29% with REE Mix), as well as by increased stomatal conductance (increase of 36%) and SPAD index (increase of 10% with Ce, 12% with La, and 15% with REE mix). Moreover, adding REEs potentiated the photosynthetic process by increasing rice leaves' N, Mg, K, and Mn concentrations (24-46%). The dose for the higher rice grain yield (an increase of 113%) was estimated for the REE mix at 0.72 kg ha-1.
RESUMO
Information on (10)B distribution in normal tissues is crucial to any further development of boron neutron capture therapy (BNCT). The goal of this study was to investigate the in vitro and in vivo boron biodistribution in B16F10 murine melanoma and normal tissues as a model for human melanoma treatment by a simple and rapid colorimetric method, which was validated by HR-ICP-MS. The B16F10 melanoma cell line showed higher melanin content than human melanocytes, demonstrating a greater potential for boronophenylalanine uptake. The melanocytes showed a moderate viability decrease in the first few minutes after BNCT application, stabilizing after 75 min, whereas the B16F10 melanoma showed the greatest intracellular boron concentration at 150 min after application, indicating a different boron uptake of melanoma cells compared to normal melanocytes. Moreover, at this time, the increase in boron uptake in melanoma cells was approximately 1.6 times higher than that in normal melanocytes. The (10)B concentration in the blood of mice bearing B16F10 melanoma increased until 90 min after BNCT application and then decreased after 120 min, and remained low until the 240th minute. On the other hand, the (10)B concentration in tumors was increased from 90 min and maximal at 150 min after application, thus confirming the in vitro results. Therefore, the present in vitro and in vivo study of (10)B uptake in normal and tumor cells revealed important data that could enable BNCT to be possibly used as a treatment for melanoma, a chemoresistant cancer associated with high mortality.
Assuntos
Terapia por Captura de Nêutron de Boro , Boro/farmacocinética , Boro/uso terapêutico , Melanócitos/metabolismo , Melanócitos/efeitos da radiação , Melanoma Experimental/metabolismo , Melanoma Experimental/radioterapia , Animais , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos da radiação , Feminino , Humanos , Melaninas/biossíntese , Melanoma Experimental/patologia , Camundongos , Camundongos Endogâmicos C57BL , Reprodutibilidade dos Testes , Distribuição TecidualRESUMO
Total mercury and selenium concentrations were determined in hair samples collected from Wari (Pacaás Novos) Indians living in Doutor Tanajura village, Gujará-Mirim city, Rondjnia State. The mercury concentrations in some samples are much higher than the values determined in samples from individuals not exposed to mercury contamination, occupationally or environmentally. The selenium concentrations are in the normal range. A correlation was observed between the mercury and selenium concentration and the values of the molar ratio approach 1 at low Hg concentrations. This fact is related to the equimolar complex formed by [(Hg-Se)n]m-Seleprotein P, which can decrease the bioavailable mercury in the organism.
Assuntos
Exposição Ambiental , Indígenas Norte-Americanos , Mercúrio/análise , Selênio/análise , Adolescente , Adulto , Idoso , Disponibilidade Biológica , Brasil , Criança , Pré-Escolar , Feminino , Cabelo/química , Humanos , Masculino , Mercúrio/química , Mercúrio/farmacocinética , Pessoa de Meia-Idade , Valores de Referência , Selênio/química , Selênio/farmacocinéticaRESUMO
Mercury is a major public health concern because of its widespread occurrence in the environment and its toxic effects on humans, mainly through fish ingestion. On the other hand, selenium is known by its antioxidant effect. For this reason, the knowledge of the correlation between mercury and selenium concentrations in fish samples can bring important information allowing a better understanding of mercury equilibrium in the inhabitants of the Amazon region. In this paper, total mercury and selenium levels were measured in carnivorous (47), omnivorous (44), and herbivorous (4) fish species caught in rivers and in an artificial lake in Cachoeira do PiriáMunicipality, situated in ParáState, Amazon region. The mercury concentration ranged from 1.15 to 13.44 nmol g(-1) and selenium from 2.44 to 14.56 nmol g(-1) for carnivorous species. For noncarnivorous species, mercury concentration ranged from 0.08 to 2.03 nmol g(-1) and selenium from 1.27 to 15.32 nmol g(-1). The molar ratios between mercury and selenium contents obtained for carnivorous and noncarnivorous species were 0.65 and 0.14, respectively. Mercury and selenium levels were positively correlated with fish body mass (weight) only for Hoplias malabaricus (n=35, R2=0.565, P<0.005 and R2=0.608, P<0.005, respectively). Selenium and mercury concentrations were statistically positively correlated only for H. malabaricus (n=35, R2=0.787, P=0.005) and Leporinus sp. (n=38, R2=0.485, P<0.005) known locally as Traíra (carnivorous) and Aracu (omnivorous), respectively.
Assuntos
Peixes , Mercúrio/análise , Selênio/análise , Poluentes Químicos da Água/análise , Animais , BrasilRESUMO
Total mercury and selenium concentrations were determined in hair samples collected from Wari (Pacaas Novos) Indians living in Doutor Tanajura village, Gujara-Mirim city, Rondjnia State. The mercury concentrations in some samples are much higher than the values determined in samples from individuals not exposed to mercury contamination, occupationally or environmentally. The selenium concentrations are in the normal range. A correlation was observed between the mercury and selenium concentration and the values of the molar ratio approach 1 at low Hg concentrations. This fact is related to the equimolar complex formed by [(Hg-Se)n]m-Seleprotein P, which can decrease the bioavailable mercury in the organism...(AU)