Red Sm:KGd(WO4)2 laser at 649†nm.

We report on the first laser operation of a Sm3+-doped monoclinic KGd(WO4)2 double tungstate crystal in the red spectral range. Pumped by a frequency-doubled optically pumped semiconductor laser (2ω-OPSL) at 479.1 nm, the 0.8 at. % Sm:KGd(WO4)2 laser generated an output power up to 17.6 mW at 649.1 nm (the 4G5/2 → 6H9/2 transition) with a slope efficiency of 16.9%, a laser threshold down to 29 mW and a linear polarization. The laser exhibited a self-pulsing behavior, delivering µs-long pulses with a repetition rate of a few kHz. The polarized spectroscopic properties of Sm3+ ions were determined as well.

Watt-level europium laser at 703 nm.

We report on a watt-level highly efficient europium laser operating at the ${^5{\rm D}_0 \to {^7}{\rm F}_4}$ transition. It is based on the stoichiometric ${\rm KEu}{({\rm WO}_4)_2}$ crystal. Under pumping by a green laser at 532.1 nm, the ${\rm KEu}{({\rm WO}_4)_2}$ laser generated a maximum peak output power of 1.11 W at ${\sim}{703}\;{\rm nm}$ with a slope efficiency of 43.2% and a linear polarization ($E\|\;{N_m}$). A laser threshold as low as 64 mW was achieved. True continuous-wave operation was demonstrated. The polarized emission properties of monoclinic ${\rm KEu}{({\rm WO}_4)_2}$ were determined.

Cellular uptake and biocompatibility of bismuth ferrite harmonic advanced nanoparticles.

Bismuth Ferrite (BFO) nanoparticles (BFO-NP) display interesting optical (nonlinear response) and magnetic properties which make them amenable for bio-oriented diagnostic applications as intra- and extra membrane contrast agents. Due to the relatively recent availability of this material in well dispersed nanometric form, its biocompatibility was not known to date. In this study, we present a thorough assessment of the effects of in vitro exposure of human adenocarcinoma (A549), lung squamous carcinoma (NCI-H520), and acute monocytic leukemia (THP-1) cell lines to uncoated and poly(ethylene glycol)-coated BFO-NP in the form of cytotoxicity, haemolytic response and biocompatibility. Our results support the attractiveness of the functional-BFO towards biomedical applications focused on advanced diagnostic imaging. FROM THE CLINICAL EDITOR: Bismuth Ferrite nanoparticles (BFO-NP) have been recently successfully introduced as photodynamic tools and imaging probes. However, how these nanoparticles interact with various cells at the cellular level remains poorly understood. In this study, the authors performed in vitro experiments to assess the effects of uncoated and PEG-coated BFO-NP in the form of cytotoxicity, haemolytic response and biocompatibility.

Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples.

A biophotonics approach based on the nonlinear optical process of second harmonic generation is presented and demonstrated on malignant human cell lines labelled by harmonic nanoparticles. The method enables independent imaging and therapeutic action, selecting each modality by simply tuning the excitation laser wavelength from infrared to visible. In particular, the generation of deep ultraviolet radiation at 270 nm allows direct interaction with nuclear DNA in the absence of photosensitizing molecules.

Harmonic nanocrystals for biolabeling: a survey of optical properties and biocompatibility.

Nonlinear optical nanocrystals have been recently introduced as a promising alternative to fluorescent probes for multiphoton microscopy. We present for the first time a complete survey of the properties of five nanomaterials (KNbO(3), LiNbO(3), BaTiO(3), KTP, and ZnO), describing their preparation and stabilization and providing quantitative estimations of their nonlinear optical response. In the light of their prospective use as biological and clinical markers, we assess their biocompatibility on human healthy and cancerous cell lines. Finally, we demonstrate the great potential for cell imaging of these inherently nonlinear probes in terms of optical contrast, wavelength flexibility, and signal photostability.