RESUMO
Three new macrocyclic trichothecenes (1-3) and five known related compounds (4-8) were isolated from the MeOH extract of a plate culture of the fungus Podostroma cornu-damae, a deadly poisonous mushroom. Miophytocen D (1) is a rearranged macrocyclic type D trichothecene, featuring a bicyclo-[6.5]dodecahydrocyclopenta[ b]chromene scaffold, and the structures of new compounds (1-3) were delineated by the combination of 1D and 2D NMR spectroscopic experiments and HRESIMS, modified Mosher's esterification, and quantum chemical ECD calculations. The isolated compounds (1-8) were evaluated for cytotoxicity against four human breast cancer cell lines (Bt549, HCC70, MDA-MB-231, and MDA-MB-468). Compounds 4, 6, and 8 exhibited significant cytotoxic effects against the breast cancer cell lines, with IC50 values in the range of 0.02-80 nM, which is stronger than doxorubicin, the positive control, and a structure-activity relationship was suggested.
Assuntos
Agaricales/patogenicidade , Micotoxinas/isolamento & purificação , Tricotecenos/isolamento & purificação , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Humanos , Espectroscopia de Ressonância Magnética , Relação Estrutura-Atividade , Tricotecenos/química , Tricotecenos/farmacologiaRESUMO
A bacterial strain, designated T20R-70T, was isolated from tomato rhizosphere soil collected in Yecheon-gun, Gyeongsangbuk-do in South Korea. Growth was observed within the ranges 10-40 °C (optimally at 28-30 °C), pH 7.0-8.0 (optimally at pH 7.0) and 0-1â% NaCl (optimally at 0â%). The 16S rRNA gene sequence showed the highest similarities with those of Lysobacter hankyongensis KTCe-2T (98.7â%), Lysobacter brunescens KCTC 12130T (98.0â%), 'Lysobacter daecheongensis' Dae08 (97.2â%) and Lysobacter oligotrophicus 107-E2T (97.1â%). The phylogenetic tree showed that strain T20R-70T formed a clade with Lysobacterhankyongensis KTCe-2T and Lysobacterbrunescens KCTC 12130T. The dominant fatty acids (>10â%) were iso-C15â:â0, iso-C16â:â0, iso-C17â:â1ω9c and summed feature 3 (including iso-C15â:â0 2-OH and/or iso-C16â:â1ω7c). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was Q-8. DNA-DNA hybridization data revealed that strain T20R-70T had a hybridization value of 42±4â% (mean±sd) to the most closely related species of the genus Lysobacter. The DNA G+C content was 63.0 mol%. The physiological, biochemical and chemotaxonomic data allowed the discrimination of the new isolate from its phylogenetic relatives. Strain T20R-70T is thus considered to be a representative of a novel species of the genus Lysobacter, for which the name Lysobactersolanacearum sp. nov. is proposed. The type strain is T20R-70T (=KACC 18656T=NBRC 111881T).
Assuntos
Lysobacter/classificação , Filogenia , Rizosfera , Microbiologia do Solo , Solanum lycopersicum/microbiologia , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/química , Lysobacter/genética , Lysobacter/isolamento & purificação , Hibridização de Ácido Nucleico , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A bacterial strain, designated T16E-198T, was isolated from the rhizosphere of tomato plant collected from a farm on Buyeo-gun, Chungcheongnam-do, South Korea. The strain was aerobic, Gram-stain-negative, rod-shaped, non-flagellated and yellow-pigmented. Strain T16E-198T was mesophilic, catalase- and oxidase-positive and with flexirubin-type pigments. A phylogenetic tree based on 16S rRNA gene sequences showed that strain T16E-198T formed a lineage with Parafilimonas terrae 5GHs7-2T, sharing highest sequence similarity of 98.4â% with it and less than 93â% with all the other validly published species. The major fatty acids were iso-C15â:â0, iso-C17â:â0 3-OH and iso-C15â:â1 G. The predominant menaquinone was MK-7. The polar lipids were phosphatidylethanolamine, one unknown aminophospholipid, five unknown aminolipids and five unknown lipids. The DNA G+C content was 41.2 mol%. On the basis of the phenotypic, phylogenetic and chemotaxonomic data presented, strain T16E-198T is considered to represent a novel species of the genus Parafilimonas, for which the name Parafilimonas rhizosphaerae sp. nov. is proposed; the type strain is T16E-198T (=KACC 18786T=JCM 31601T).
Assuntos
Bacteroidetes/classificação , Filogenia , Rizosfera , Microbiologia do Solo , Solanum lycopersicum/microbiologia , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
An aerobic, Gram-stain-negative, non-spore-forming, non-flagellated, rod-shaped or filamentous bacterial strain, T16R-86T, was isolated from rhizosphere of a tomato plant collected from a farm on Buyeo-gun, Chungcheongnam-do, South Korea. It grew at the temperature range 10-37 °C (optimum, 28 °C) and pH range 6.0-9.0 (optimum, pH 7.0), and tolerated up to 2â% (w/v) NaCl. According to 16S rRNA gene sequence analysis, strain T16R-86T shared the highest similarity with Chitinophaga barathri YLT18T (96.8â%) and C. pinensis DSM 2588T (96.7â%), forming a subcluster with C. barathri YLT18T, C. cymbidii R156-2T and C. niabensis JS13-10T in the phylogenetic tree. The major fatty acids were iso-C15â:â0, C16â:â1ω5c and iso-C17â:â0 3-OH. The predominant respiratory quinone was menaquinone MK-7. Polar lipids were phosphatidylethanolamine, five unknown aminolipids, an unknown aminophospholipid, one unknown phospholipid and two unknown lipids. The DNA G+C content was 53.6 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed that strain T16R-86T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga rhizosphaerae sp. nov. is proposed. The type strain is T16R-86T (=KACC 18790T=JCM 31600T).
Assuntos
Bacteroidetes/classificação , Filogenia , Rizosfera , Microbiologia do Solo , Solanum lycopersicum/microbiologia , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A yellow, aerobic, Gram-stain-negative, non-motile, rod-shaped and non-flagellated bacterial strain, designated T16R-129T, was isolated from the rhizosphere of a tomato plant collected at a farm located on Buyeo-gun of Chungcheongnam-do, South Korea. Strain T16R-129T grew at 15-40 °C and pH 7.0-9.0, and did not require NaCl for growth. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T16R-129T clustered with members of the genus Terrimonas, and it shared highest similarity with Terrimonas arctica R9-86T (96.1â%), Terrimonas pekingensis QHT (95.9â%), Terrimonas lutea DYT (94.9â%), Terrimonas crocea M1-33108T (95.4â%) and Terrimonas rhizosphaerae CR94T (95.3â%). The major isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C15â:â0, iso-C17â:â0 3-OH and iso-C15â:â1 G. The polar lipids of strain T16R-129T were phosphatidylethanolamine, two unidentified aminolipids, two unidentified aminophospholipids and five unidentified polar lipids. The G+C content of the genomic DNA was 46.0 mol%. On the basis of data from this polyphasic taxonomic study, strain T16R-129T represents a novel species in the genus Terrimonas, for which the name Terrimonas terrae sp. nov. is proposed; the type strain is T16R-129T (=KACC 18787T=JCM 31603T).
Assuntos
Bacteroidetes/classificação , Filogenia , Rizosfera , Solanum lycopersicum/microbiologia , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
Calvatia species, also known as puffball mushrooms, are common sources of food worldwide. Calvatia nipponica (Agaricaceae) is one of the most rare species in the Calvatia genus. It was first validated in 2008. Due to its scarcity, C. nipponica has never been chemically investigated. Its recent discovery in Korea allowed to conduct this study, and using bioactivity-guided fractionation for antiangiogenic activity, chemical investigation of the MeOH extract of the fruiting bodies of C. nipponica led to the isolation of five alkaloids (1-5) and two phenolic compounds (6-7). This is the first study to report the chemical investigation of C. nipponica, and compound 1 was previously reported as chemically synthesized only until our report of its isolation and identification from natural sources. The structure of 1 was determined by spectroscopic analysis by 1D and 2D NMR spectra and HR-MS. All compounds (1-7) were tested for inhibition of angiogenesis using human umbilical vein endothelial cells (HUVECs). Compounds 2, 4 and 5 significantly inhibited the promotion of angiogenesis in HUVECs. Compound 5 showed the most potent inhibition via downregulation of VEGF, p38 and ERK signaling pathways. These results suggested that the rare mushroom C. nipponica might be beneficial in antiangiogenesis therapy for cancer treatment.
Assuntos
Agaricales/química , Inibidores da Angiogênese/farmacologia , Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos , Neovascularização Patológica/tratamento farmacológico , Inibidores da Angiogênese/química , Inibidores da Angiogênese/isolamento & purificação , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Humanos , Estrutura Molecular , Neovascularização Patológica/patologia , Relação Estrutura-AtividadeRESUMO
A rod-shaped, yellow, Gram-stain-negative, non-flagellated, aerobic bacterium, designated 5516J-17T, was isolated from an air sample collected from Jeju Island, Republic of Korea. It grew in the temperature range of 10-37 °C (optimum 28-30 °C), pH 6.0-11.0 (optimum, pH 7.0) and 0-1 % NaCl (w/v). Phylogenetic trees generated using 16S rRNA gene sequences revealed that strain 5516J-17T belongs to the genus Spirosoma, showing 96.9 % sequence similarity to the most closely related species, Spirosoma linguale DSM 74T. The cellular fatty acids comprised large amounts (>10 % of total fatty acids) of summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:1ω5c, and moderate amounts (5-10 % of total fatty acids) of iso-C17:0 3-OH, iso-C15:0 and C16:0. The DNA G+C content was 55.7 mol % and MK-7 was the predominant isoprenoid quinone. Polar lipids were phosphatidylethanolamine, two unknown aminophospholipids, one unknown aminolipid and one unknown lipid. On the basis of this phenotypic and polyphasic taxonomy study, it is suggested that strain 5516J-17T represents a novel species within the genus Spirosoma, with the proposed name Spirosoma aerophilum. The type strain is 5516J-17T (= KACC 17323T = DSM 28388T = JCM 19950T).
Assuntos
Movimentos do Ar , Cytophagaceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , Cytophagaceae/genética , Cytophagaceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Fosfatidiletanolaminas/química , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
Strain 5GH22-11T, which was isolated from greenhouse soil in the Yangpyeong region, Gyeonggi province, Republic of Korea, was characterized to be an aerobic, Gram-stain-negative, flagellated, rod-shaped bacterium. It could grow at temperatures from 10 to 33 °C (optimum of 28-30 °C), in the pH range of 6.0-10.0 (optimum of pH 7.0) and without NaCl. 16S rRNA gene sequence analysis showed that strain 5GH18-14T showed the highest sequence similarities with Lysobacter niabensis GH34-4T (98.6 %), Lysobacter yangpyeongensis GH19-3T (98.1 %), 'Lysobacter fragariae' THG-DN8.7 (97.9 %), Lysobacter terrae THG-A13T (97.3 %), 'Lysobacter rhizosphaerae' THG-DN8.3 (97.2 %), 'Lysobacter tyrosinelyticus' THG-DN8.2 (97.2 %) and Lysobacter oryzae YC6269T (97.2 %), revealing less than 95.5 % sequence similarities with all other species with validly published names. Phylogenetic trees also indicated that strain 5GH18-14T formed a compact subcluster with L. niabensis GH34-4T, L. yangpyeongensis GH19-3T, L. terrae THG-A13T and L. oryzae YC6269T within the genus Lysobacter. The predominant quinone of strain 5GH18-14T was Q-8. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylmonomethylethanolamine in large amounts, and moderate or small amounts of three unknown phospholipids and two unknown aminophospholipids. DNA-DNA hybridization values with closely related species were below 70 %. The DNA G+C content was 65.9âmol%. Based on the phylogenetic, physiological and chemotaxonomic data, it has been demonstrated that strain 5GH18-14T represents a novel species of the genus Lysobacter, for which the name Lysobacter terricola sp. nov. is proposed. The type strain is 5GH18-14T ( = KACC 16954T = JCM 30862T).
RESUMO
A Gram-stain-negative, rod-shaped, non-flagellated, mesophilic, yellow-pigmented, aerobic bacterium, designated strain KIS20-3T, was isolated from a soil sample of Baengnyeong Island in Onjin county, Republic of Korea. According to 16S rRNA gene sequence analysis, the isolate showed highest similarities with Flavitalea populi HY-50RT (94.5 %), Niastella populi THYL-44T (94.2 %) and Flavitalea gansuensis JCN-23T (93.7 %). The neighbour-joining tree based on 16S rRNA gene sequences showed that strain KIS20-3T formed a subcluster with members of the genus Flavitalea, and this subcluster was closely related to members of the genera Niastella, Segetibacter and Parasegetibacter within the family Chitinophagaceae. The major fatty acids of strain KIS20-3T were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G, and the predominant isoprenoid quinone was menaquinone 7. The polar lipid profile comprised large amounts of phosphatidylethanolamine and one unknown polar lipid, and moderate or small amounts of four unknown aminophospholipids, two unknown aminolipids, three unknown lipids and one unknown phospholipid. The G+C content of the DNA of strain KIS20-3T was 55.7âmol%. On the basis of the results of the polyphasic characterization presented in this study, it is concluded that strain KIS20-3T represents a novel species of the genus Flavitalea, for which the name Flavitalea soli sp. nov. is proposed. The type strain is KIS20-3T ( = KACC 17319T = JCM 19937T).
RESUMO
A bacterial strain, designated T16R-265T, was isolated from rhizosphere of tomato in Buyeo-gun, Chungcheongnam-do, South Korea. The strain was Gram-stain-negative, aerobic, non-flagellated and rod- or long-rod-shaped. It grew at the range of 10-40 °C (optimum 28-30 °C), pH 6.0-9.0 (optimum pH 7.0) and with 0-3 % (w/v) NaCl (optimum 0 %). 16S rRNA gene sequence analysis showed that strain T16R-265T had the highest sequence similarity (98.0 %) with Flavitalea soli KIS20-3T and low sequence similarities of below 95 % with all other taxa. The phylogenetic tree revealed that the strain formed a robust monophyletic line with Flavitalea soli KIS20-3T separable from other clades. The predominant quinone of strain T16R-265T was MK-7, the polar lipids consisted of large amounts of phosphatidylethanolamine and an unknown lipid, and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. DNA-DNA hybridization values with Flavitalea soli KACC 17319T were below 70 %. The DNA G+C content of strain T16R-265T is 46.2 mol%. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses, strain T16R-265T represents a novel species of a new genus, for which the name Pseudoflavitalea rhizosphaerae gen. nov., sp. nov. is proposed. The type strain of the type species is T16R-265T (=KACC 18655T=NBRC 111880T). We also propose the reclassification of Flavitalea soli as Pseudoflavitalea soli comb. nov. (type strain KIS20-3T=KACC 17319T=JCM 19937T).
Assuntos
Filogenia , Rizosfera , Solanum lycopersicum/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Hibridização de Ácido Nucleico , Fosfatidiletanolaminas/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A Gram-stain-negative, variably shaped, non-flagellated, yellow-pigmented, aerobic bacterium, designated strain SGM2-10(T), was isolated from a paddy soil sample from the Suwon region, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was related most closely to Parasegetibacter luojiensis RHYL-37(T). Strain SGM2-10(T) showed highest 16S rRNA gene sequence similarity to P. luojiensis RHYL-37(T) (95.1â%), Flavitalea populi HY-50R(T) (95.0â%) and Flavitalea gansuensis JCN-23(T) (94.4â%). No other species in the family Chitinophagaceae shared more than 94.1â% 16S rRNA gene sequence similarity with strain SGM2-10(T). The major fatty acids of strain SGM2-10(T) were iso-C15â:â0, iso-C15â:â1 G and iso-C17â:â0 3-OH. The only menaquinone was MK-7. The polar lipids comprised phosphatidylethanolamine, seven unknown lipids and ten unknown aminolipids. The G+C content of the DNA of strain SGM2-10(T) was 46.7 mol%. On the basis of the results of the polyphasic characterization presented in this study, it is concluded that strain SGM2-10(T) represents a novel species of the genus Parasegetibacter, for which the name Parasegetibacter terrae sp. nov. is proposed. The type strain is SGM2-10(T) (â=âKACC 17341(T)â=âJCM 19942(T)). The description of the genus Parasegetibacter has also been emended.
Assuntos
Bacteroidetes/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfatidiletanolaminas/química , Pigmentação , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
An aerobic, Gram-stain-negative, non-flagellated, long rod or rod-shaped bacterial strain, 5GH22-11(T), was isolated from a soil sample of a greenhouse in Gongju, Republic of Korea. According to 16S rRNA gene sequence analysis, strain 5GH22-11(T) showed the highest sequence similarities with Niastella populi THYL-44(T) (97.1%), Niastella koreensis GR20-10T (95.7%) and Niastella yeongjuensis GR20-13(T) (95.6%), and < 3% sequence similarity with all other species with validly published names. The phylogenetic tree also showed strain 5GH22-11(T) formed a compact cluster with members of the genus Niastella. DNA-DNA hybridization revealed strain 5GH22-11(T) is a novel species, showing far less than 70% DNA-DNA relatedness with Niastella populi THYL-44(T). Strain 5GH22-11(T) contained iso-C15 : 0 (34.3%), iso-C17 : 0 3-OH (24.8%) and iso-C15 : 1 G (18.2%) as the major fatty acids; phosphatidylethanolamine, six unknown aminolipids, two unknown lipids and one unknown phospholipid as the polar lipids; and MK-7 as the predominant isoprenoid quinone. The DNA G+C content was 41.4 mol%. Based on these phylogenetic, physiological and chemotaxonomic data, it was demonstrated that strain 5GH22-11(T) represents a novel species of the genus Niastella, for which the name Niastella gongjuensis sp. nov. is proposed. The type strain is 5GH22-11(T) ( = KACC 17339(T) [corrected] = JCM 19941(T)).
Assuntos
Bacteroidetes , Técnicas de Tipagem Bacteriana , Bacteroidetes/classificação , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Dados de Sequência Molecular , Hibridização de Ácido Nucleico/genética , Fosfolipídeos/análise , Filogenia , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Solo , Microbiologia do SoloRESUMO
A novel strain, designated 5GHs31-2(T), was isolated from a greenhouse soil sample collected from Yongin city, South Korea. Cells of strain 5GHs31-2(T) were Gram-stain-negative, rod-shaped, polar flagellated and yellow-pigmented. The isolate was aerobic, catalase-negative and oxidase-positive and grew optimally at 28-30 °C and pH 7.0. Strain 5GHs31-2(T) revealed the highest 16S rRNA gene sequence similarities with Fulvimonas soli LMG 19981(T) (97.6%), Dyella thiooxydans ATSB10(T) (97.5%) and Frateuria terrea VA24(T) (97.4%). Furthermore, the neighbour-joining tree showed that strain 5GHs31-2(T) was a representative of a member of the genus Fulvimonas. Strain 5GHs31-2(T) contained iso-C(16â:â0), summed feature 9 (iso-C(17â:â1)ω9c and/or C(16â:â0) 10-methyl), iso-C(15â:â0) and iso-C(17â:â0) as the major fatty acids, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an aminolipid as the main polar lipids and Q-8 as the predominant quinone. The genomic DNA G+C content of 5GHs31-2(T) was 73.0 mol%. According to DNA-DNA hybridization experiments, DNA-DNA relatedness values between strain 5GHs31-2(T) and its closest phylogenetic neighbours were below 70%. Based on the taxonomic data, strain 5GHs31-2(T) represents a novel species of the genus Fulvimonas, for which the name Fulvimonas yonginensis sp. nov. is proposed. The type strain is 5GHs31-2(T) (â=âKACC 16952(T)â=âDSM 28344(T)).
Assuntos
Filogenia , Microbiologia do Solo , Xanthomonadaceae/classificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/química , Xanthomonadaceae/genética , Xanthomonadaceae/isolamento & purificaçãoRESUMO
Two bacterial strains, designated JS4-4(T) and SHS5-24(T), were isolated from forest soil of Jeju Island and fresh water of Seoho lake in Suwon city, respectively, South Korea. Both strains were Gram-stain-negative, aerobic, motile rods. Strains JS4-4(T) and SHS5-24(T) showed high sequence similarities (97.6-95.8â%) and (96.5-95.6â%), respectively, to the members of the genus Undibacterium. The sequence similarity between strains JS4-4(T) and SHS5-24(T) was 97.0â%. A phylogenetic tree showed that these strains fell within the radius of the genus Undibacterium. The main fatty acids of strains JS4-4(T) and SHS5-24(T) were summed feature 3 (C16â:â1ω7c and/or C16â:â1ω6c) (50.1 and 58.7â%, respectively) and C16â:â0 (28.3 and 24.5â%, respectively). Both strains had ubiquinone 8 as the only respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. Strain JS4-4(T) showed <70â% DNA-DNA hybridization with members of the genus Undibacterium. Thus, based on the evidence of a polyphasic study, it is proposed that strains JS4-4(T) and SHS5-24(T) represent two novel species, for which the names Undibacterium jejuense sp. nov. (type strain JS4-4(T)â=âKACC 12607(T)â=âNBRC 108922(T)) and Undibacterium seohonense sp. nov. (type strain SHS5-24(T)â=âKACC 16656(T)â=âNBRC 108929(T)) are proposed.
Assuntos
Lagos/microbiologia , Oxalobacteraceae/classificação , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Oxalobacteraceae/genética , Oxalobacteraceae/isolamento & purificação , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A Gram-stain-negative, non-motile, aerobic rod, designated 8604S-37(T), was isolated from an outdoor air sample collected in Suwon region, Republic of Korea. The isolate was catalase-positive and oxidase-negative. 16S rRNA gene sequence analysis indicated that strain 8604S-37(T) was most closely related to Diaphorobacter nitroreducens NA10B(T) and Diaphorobacter oryzae RF3(T), with which it shared 97.0% 16S rRNA gene sequence similarity. Strain 8604S-37(T) grew at 10-37 °C (optimum 28 °C; no growth occurred at 4 or 40 °C) and pH 5-9 (optimum pH 7). The strain tolerated up to 3% NaCl (w/v) (optimum 0%). The only respiratory quinone was ubiquinone-8 (Q-8). The polar lipid profile comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and one unknown aminolipid. The major fatty acids were C16 : 0, summed feature 3 (comprising C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), summed feature 8 (C(18 : 1)ω6c and/or C(18 : 1)ω7c) and C(17 : 0) cyclo, and the predominant hydroxy fatty acid was C(10 : 0) 3-OH. Strain 8604S-37(T) exhibited less than 50% DNA-DNA relatedness with Diaphorobacter nitroreducens KACC 13856(T) and Diaphorobacter oryzae KACC 16857(T). The DNA G+C content was 65 mol%. Strain 8604S-37(T) represents a novel species of the genus Diaphorobacter, for which the name Diaphorobacter aerolatus sp. nov. is proposed. The type strain is 8604S-37(T) (â=âKACC 16536(T)â=âNBRC 108926(T)). An emended description of the genus Diaphorobacter is also proposed.
Assuntos
Microbiologia do Ar , Comamonadaceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , Comamonadaceae/genética , Comamonadaceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A Gram-stain-negative bacterium, strain 5410S-62T, was isolated from an air sample collected in Suwon, Republic of Korea. It was aerobic, motile, mesophilic and formed rod-shaped cells. Colonies on R2A agar were convex, circular and pale orange with entire margins. Growth occurred at pH 5-9 (optimally at pH 7) and at 10-40 °C (optimally at 28 °C). It did not grow in the presence of 1% NaCl. Comparative analyses of 16S rRNA gene sequences demonstrated that the novel strain was closely related to members of the genus Noviherbaspirillum. Strain 5410S-62T showed the highest sequence similarity (98.2%) to Glaciimonas singularis A2-57T. It also showed high 16S rRNA gene sequence similarity (98.1-95.6%) to members of the genus Noviherbaspirillum (98.1% to Noviherbaspirillum aurantiacum SUEMI08T, 97.8% to Noviherbaspirillum soli SUEMI10T and Noviherbaspirillum canariense SUEMI03T, 97.6% to Noviherbaspirillum psychrotolerans PB1T and 95.6% to Noviherbaspirillum malthae CC-AFH3T). The strain contained summed feature 3 (C16:1ω6c and/or C16:1ω7c), C16:0 and summed feature 8 (C18:1ω6c and/or C18:1ω7c) as major fatty acids, Q-8 as the only ubiquinone and large amounts of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. Strain 5410S-62T revealed less than 70% DNA-DNA relatedness with the type strains of closely related species of the genera Noviherbaspirillum and Herbaspirillum and Glaciimonas singularis. Based on the physiological, biochemical and chemotaxonomic data obtained in this study, it is proposed that strain 5410S-62T represents a novel species, Noviherbaspirillum suwonense sp. nov., with 5410S-62T (=KACC 16657T=âNBRC 108944T) as the type strain.
Assuntos
Microbiologia do Ar , Oxalobacteraceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , Oxalobacteraceae/genética , Oxalobacteraceae/isolamento & purificação , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/químicaRESUMO
A bacterial strain, designated RHs22(T), was isolated from a soil sample cultivated with rice in the Suwon region of South Korea. The cells were aerobic, Gram-stain-negative, non-spore-forming, non-flagellated rods or occasionally filaments. The strain grew at 10-37 °C (optimum, 28-30 °C), at pH 5.0-10.0 (optimum, 7.0) and in the presence of 0-1â% (w/v) NaCl (optimum, 0â%). Phylogenetically, the strain was closely related to members of the genus Spirosoma, as its 16S rRNA gene sequence had similarity of 90.3-92.1â% with respect to those of members of the genus Spirosoma, showing the highest sequence similarity with Spirosoma panaciterrae DSM 21099(T). Strain RHs22(T) revealed relatively low sequence similarities of less than 90â% with all the other species with validly published names. It contained MK-7 as the predominant menaquinone and summed feature 3 (C16â:â1ω6c and/or C16â:â1ω7c), C16â:â1ω5c, iso-C15â:â0 and iso-C17â:â0 3-OH as the main fatty acids. The polar lipids of strain RHs22(T) were phosphatidylethanolamine, one unknown aminolipid, two unknown aminophospholipids, one unknown phospholipid and five unknown lipids. The DNA G+C content was 57.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data obtained in this study indicate that strain RHs22(T) represents a novel species of the genus Spirosoma, for which the name Spirosoma oryzae sp. nov. is proposed. The type strain is RHs22(T) (â=âKACC 17324(T)â=âDSM 28354(T)). An emended description of the genus Spirosoma is also proposed.
Assuntos
Cytophagaceae/classificação , Oryza/microbiologia , Filogenia , Microbiologia do Solo , Técnicas de Tipagem Bacteriana , Composição de Bases , Cytophagaceae/genética , Cytophagaceae/isolamento & purificação , DNA Bacteriano/genética , Ácidos Graxos/química , Dados de Sequência Molecular , Fosfolipídeos/química , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMO
A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated KIS14-15(T), was isolated from forest soil of Baengnyeong Island in the Yellow Sea in Korea and its taxonomic position was investigated by using a polyphasic study. Strain KIS14-15(T) grew optimally at 30 °C, at pH 6.0-7.0 and without NaCl. In the neighbour-joining phylogenetic tree based on 16S rRNA gene sequences, strain KIS14-15(T) formed a cluster with the strains of Reyranella massiliensis with a bootstrap resampling value of 100%. Strain KIS14-15(T) exhibited 16S rRNA gene sequence similarity values of 98.0% to R. massiliensis 521(T) and of less than 89% to the type strains of other taxa. The mean level of DNA-DNA relatedness between strain KIS14-15(T) and R. massiliensis KACC 16548(T) was 21% (reciprocal, 24%). The predominant ubiquinone found in strain KIS14-15(T) and R. massiliensis KACC 16548(T) was ubiquinone-10 (Q-10). The predominant fatty acids of strain KIS14-15(T) and R. massiliensis KACC 16548(T) were C(18:1)ω7c, C(18:1) 2-OH and 11-methyl C(18:1)ω7c. Total polar lipids of strain KIS14-15(T) were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown aminolipid and one unknown lipid. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, demonstrated that strain KIS14-15(T) is distinguishable from R. massiliensis. On the basis of the data presented, strain KIS14-15(T) is considered to represent a novel species of the genus Reyranella, for which the name Reyranella soli sp. nov. is proposed. The type strain is KIS14-15(T) (â=âKACC 13034(T)â=NBRC 108950(T)).
Assuntos
Alphaproteobacteria/classificação , Filogenia , Microbiologia do Solo , Alphaproteobacteria/genética , Alphaproteobacteria/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Dados de Sequência Molecular , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Árvores/microbiologia , Ubiquinona/análiseRESUMO
An aerobic, Gram-stain-negative, motile, mesophilic bacterium, designated strain 7515T-07(T), was isolated from an air sample in the Taean region, Republic of Korea. The strain grew at 4-40 °C (optimum, 30 °C) and pH 5.0-9.0 (optimum, pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 7515T-07(T) was related to members of the genus Roseomonas and had highest 16S rRNA gene sequence similarity with Roseomonas ludipueritiae 170/96(T) (96.7%). 16S RNA gene sequence similarity between strain 7515T-07(T) and Roseomonas gilardii ATCC 49956(T) (the type species of the genus Roseomonas) was 93.4%. Strain 7515T-07(T) contained Q-10 as the ubiquinone and C18:1ω7c and C19:0 cyclo ω8c as the dominant fatty acids (>10%). The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and two unidentified aminolipids. The DNA G+C content was 73.0 mol%. Combined data from phenotypic, phylogenetic and chemotaxonomic studies demonstrated that strain 7515T-07(T) is a representative of a novel species of the genus Roseomonas, for which the name Roseomonas aerophila sp. nov. is proposed. The type strain is 7515T-07(T) (=KACC 16529(T)=NBRC 108923(T)).
Assuntos
Microbiologia do Ar , Methylobacteriaceae/classificação , Filogenia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/análise , Methylobacteriaceae/genética , Methylobacteriaceae/isolamento & purificação , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , República da Coreia , Análise de Sequência de DNA , Ubiquinona/análiseRESUMO
A new trimeric hispidin derivative, phellinstatin, was isolated from a culture broth of the medicinal fungus Phellinus linteus and its structure was established by various spectral analysis. Phellinstatin strongly inhibited Staphylococcus aureus enoyl-ACP reductase with an IC(50) of 6 µM and also showed antibacterial activity against S. aureus and MRSA.