Antibacterial effect of green tea infusion used as a mouthwash on saliva and bacterial plaque: a randomized controlled trial.

The objective of this study was to evaluate the antibacterial effect of a green tea infusion used as a mouthwash. In this double-blind, randomized controlled study, simple randomization was used to divide 28 students, 18 to 40 years of age, into 2 groups (n = 14): green tea (intervention) or distilled water (placebo). A 13% green tea infusion was prepared with 13 g of green tea (commercially fractionated) in 100 mL of saline solution at a temperature of approximately 90°C. The liquid was poured into sterile glass containers, as was the distilled water for the control group. The antibacterial effect was determined by counting the colony-forming units in cultures of plaque and saliva samples, which were obtained before and immediately after the students rinsed their mouth once with 10 mL of the green tea or control solution. The data were subjected to t tests to analyze differences before and after the intervention and between groups (green tea versus distilled water) (P < 0.05). The analysis showed statistically significant differences between the groups (P < 0.05), with participants in the green tea group demonstrating greater reductions in the numbers of colony-forming units. Green tea mouthwash had an antibacterial effect on saliva and bacterial plaque, suggesting that green tea mouthwash could be a beneficial addition to standard oral hygiene measures. Trial registration: ClinicalTrials.gov Identifier NCT04410666.

Antibiotic resistance of Staphylococcus aureus isolated from the oral cavity of paraguayan participants from Coronel Oviedo, year 2021

A cross-sectional study was carried out to identify the resistance profile of Staphylococcus aureus colonizing the oral cavity of patients. Sampling was carried out with sterile swabs. Mannitol-positive colonies were cultured on blood agar, identifying S. aureus by conventional biochemical tests that included positive results for catalase, coagulase, DNAse and latex agglutination test for S. aureus. The resistance to 11 antibiotics was tested. Forty-three subjects were included; 83.7% presented oral pathologies, and 6.9% had a history of hospitalization, 18.6% mentioned having used antibiotics in the last three months. S. aureus was isolated in 3 patients; the 3 isolates presented resistance to penicillin, while 2 of the three isolates were resistant to oxacillin and cefoxitin. Resistance to erythromycin and clindamycin was also present in one patient. Two of the cases presented resistance to various drugs and didn´t report any risk factors.

Se realizó un estudio transversal para identificar el perfil de resistenia de S. aureus que se encuentra colonizando la cavidad bucal de los pacientes. La toma de muestras se realizó con hisopos estériles. Se cultivaron colonias positivas a manitol en agar sangre, identificándose S. aureus mediante pruebas bioquímicas convencionales que incluyeron resultados positivos para catalasa, coagulasa, ADNasa y aglutinación en látex para S. aureus. Se probó la resistencia a 11 antibióticos. Se incluyeron cuarenta y tres sujetos; el 83,7% presentó patología bucal y el 6,9% tenía antecedentes de hospitalización, el 18,6% mencionó haber utilizado antibióticos en los últimos tres meses. Se aisló S. aureus en 3 pacientes; los 3 aislados presentaron resistencia a penicilina, mientras que 2 de los tres aislados fueron resistentes a oxacilina y cefoxitina. Un paciente también presentó resistencia a eritromicina y clindamicina. Dos de los casos presentaron resistencia a diversos fármacos, sin haber reportado factores de riesgo.

Surgical diagnosis and management of intestinal obstruction due to Ascaris lumbricoides.

BACKGROUND: Ascariasis continues to be one of the most important parasitic diseases in terms of its burden and complications in children in the developing world. METHODS: Case report and literature review (Medline, SCI, and LILACS). RESULTS: We report herein a case in which a Paraguayan infant presented with one of these complications: An intestinal obstruction due to Ascaris lumbricoides being diagnosed during surgery. The patient was managed with a conservative protocol for the extraction of the parasites using liquid petrolatum administered through a nasogastric tube followed with extensive water irrigation through the tube, in conjunction with the administration of piperazine as antiparasitic treatment. CONCLUSIONS: This case, as with others reported previously, shows that this complication can be managed successfully without major intestinal surgery. Early recognition of this condition, based on local prevalence, can prevent serious surgical complications, morbidity, and mortality associated with intestinal obstruction due to A. lumbricoides.