Isolation of chitosan and hydroxyapatite from waste edible white garden snail shells and their sensing applications towards industrial Congo red dye detection: Greener approach.

Biomaterials like chitosan, hydroxyapatite have been used in biomedical and pharmaceutical field, due to its valuable biochemical and physiological properties. In current work firstly our group has isolated a polysaccharide chitosan along with hydroxyapatite biomaterial from the same source by varying the process condition via greener approach. We have adapted greener approach for the isolation of chitosan within a short period of time and this is the very first report for the isolation of both chitosan and hydroxyapatite simultaneously from the same waste edible garden snail shells. Both these materials were thoroughly characterized by using UV, FT-IR, SEM techniques. Among synthetic colourants, congo red dye is recognized as carcinogens, which are usually used in the textile manufacturing. Interestingly, one of our biomaterial hydroxyapatite has shown good selectivity towards Congo red dye. The sensitivity range was obtained from 10 to 100 µM within the LOD of 101.52 nM. The developed sensor has been tested for various industrial effluents and shown good agreement with our results. Meanwhile these chitosan and hydroxyapatite have also been used as capping agent for the preparation of stable gold nanoparticles.

Simple enzyme based fluorimetric biosensor for urea in human biofluids.

As an important biomarker for renal related diseases, detection of urea is playing a vital role in human biofluids on clinical diagnosis concern. In this work, a synthetic salicyaldehyde based imine fluorophore was synthesized using sonication method and conjugated with urease which was used as fluorescent biosensor for the detection of urea in serum samples. This enzyme based biosensor has shown a good selectivity and sensitivity towards urea with the linear range from 2 to 80 mM and the detection limit of 73 µM. The sensing response obtain is highly agreeing with existing analytical technique for urea detection which strongly recommends this biosensor for clinical application.

Label free optical biosensor for insulin using naturally existing chromene mimic synthesized receptors: A greener approach.

We currently reporting a simple and first label free optical insulin biosensor using a novel cum naturally existing Chromene mimic receptors via one pot greener methodology. Among synthesized 4H- Chromene derivatives, 2-amino-5,7-dihydroxy-4-(4-nitrophenyl)-4H-chromene-3-carbonitrile (Compound 1c) is showed a very good colorimetric response for insulin within the linear range of 20 fM -500 pM along with theoretically measured LOD of 15.38 fM based on the increment in absorbance. Meanwhile 2-amino-5, 7-dihydroxy-4-(pyren-2-yl)-4H-chromene-3-carbonitrile (Compound 1a) is depicted an excellent quenching in fluorescence response for insulin with LOD of 7.07 fM and along with wide working range from 10 fM -600 pM. The developed biosensor is tested for the detection of insulin in human blood serum using direct as well as standard addition method and the recovery results are showed a very good agreement with standard clinical chemiluminescent assay method. Hence, this developed biosensor is more reliable for clinical detection of Insulin and so a good analytical method for Diabetes diagnosis within a short period of time.

Fluorimetric chemodosimeter for the detection of capsaicinoids in food matrices.

Capsaicin is a major pungent capsaicinoids in chili pepper and it causes duodenal, liver, stomach and gastric cancer in human. Hence, the detection of capsaicinoids becomes important on health issues concern. Here we are reporting, the first organic molecule based fluorimetric sensor for capsaicin detection using simple fluorophore 4-3-(pyren-2-yl-acryloyl) phenyboronic acid (PAPA), which was synthesized via greener microwave method. The probe has detected the capsaicin selectively in presence of other biomolecules in human biofluids through the intramolecular charge transfer mechanism and supported with DFT studies. The sensor has shown an excellent response towards capsaicin from 2 to 40 µM and the limit of detection of 12.84 nM. Real time analysis was done in various food matrices having capsaicinoids and the results have clearly shown good agreement with our optimized data and it also evinced that the developed sensor can be applied to detect the level of pungency of capsaicinoids.

Optical Immunosensor for the Detection of Listeria monocytogenes in Food Matrixes.

In this paper, simple imine-based organic fluorophore 4-amino-3-(anthracene-9 yl methyleneamino) phenyl (phenyl) methanone (APM) has been synthesized via a greener approach and the same was used to construct a fluorescent immunoassay for the detection of Listeria monocytogenes (LM). A monoclonal antibody of LM was tagged with APM via the conjugation of the amine group in APM and the acid group of anti-LM through EDC/NHS coupling. The designed immunoassay was optimized for the specific detection of LM in the presence of other interfering pathogens based on the aggregation-induced emission mechanism and the formation of aggregates and their morphology was confirmed with the help of scanning electron microscopy. Density functional theory studies were done to further support the sensing mechanism-based changes in the energy level distribution. All photophysical parameters were measured by using fluorescence spectroscopy techniques. Specific and competitive recognition of LM was done in the presence of other relevant pathogens. The immunoassay shows a linear appreciable range from 1.6 × 106-2.7024 × 108 cfu/mL using the standard plate count method. The LOD has been calculated from the linear equation and the value is found as 3.2 cfu/mL, and this is the lowest LOD value reported for the detection of LM so far. The practical applications of the immunoassay were demonstrated in various food samples, and their accuracy obtained was highly comparable with the standard existing ELISA method.

Optical detection of thiocyanate in human saliva based on the colorimetric response of (2-(2-hydroxyphenyl)-1H-benzo[d]imidazol-5-yl)(phenyl) methanone (HBPM)/Co2+ ions conjugate.

Simple colorimetric biosensor was designed for the quantification SCN- ions based on the principle of colorimetric reactions between Co2+ and SCN- ions reaction using synthesized chromophore (2-(2-hydroxyphenyl)-1H-benzo[d]imidazol-5-yl)(phenyl) methanone (HBPM)/Co2+ ions conjugate which was synthesized via greener ultrawave sonication method. The structural characterization of the HBPM chromophore was confirmed by using NMR, ESI-MASS spectral techniques and the photophysical properties, sensor studies were done by using UV-Vis Spectrophotometer. Our designed HBPM/Co2+ ions conjugates have selectively detected SCN- ions qualitatively and quantitatively in the presence of other human salivary interferents. As per clinical point of view, three different ranges of SCN- ions from 0.1 to 2 mM for normal, 3 to 10 mM for disease, and 100-600 µM for below normal ranges were tested successfully by our developed sensor and the LoD was calculated as 5.43 nM. The real potent application of the developed biosensor was tested in human salivary samples of both smokers and non-smokers under different ages and obtained results shown good agreement with existing clinical methods.