[Comparative proteomics of pancreatic cancer].

OBJECTIVE: To identify differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues by comparative proteomics analysis. METHODS: Six pairs pancreatic tumor tissues and the adjacent noncancerous tissues were obtained and selected for two-dimensional polyacrylamide gel electrophoresis (2-DE) analysis. The differentially expressed proteins were identified using the PDQuest 2-D analysis software. After cutting and enzymolysis, the differentially expressed proteins were analysised by ESI-Q-TOF mass spectrometer. The MS/MS data were acquired and searched in the Swiss-Prot database using MASCOT software. Altered expression of representative proteins was validated by immunohistochemical staining. RESULTS: A total of 97 points were identified as differentially expressed by two-dimensional gel electrophoresis, of which 31 were successfully identified by ESI-Q-TOF mass spectrometer. Among the proteins identified by mass spectrometer, 23 were upregulated and the other 8 were downregulated. These proteins were involved in different functional processes and protein families: glucolysis, hyaluronidase, I, II-phase metabolic enzymes, metalloproteinase, cytoskeleton, Ca2+ metabolism and so on. Immunohistochemical staining revealed an overexpression of HYAL1 and low expression of CYP2C8 and GSTM2 in pancreatic tumor. CONCLUSION: The differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues can be identified by comparative proteomics approach. The selected proteins will provide a valuable clue and help for further research.

Inflammatory colonic obstruction developed as a complication of acute gangrenous cholecystitis

Inflammatory colonic obstruction has rarely been reported as a complication of acute gangrenous cholecystitis. In this paper, we report a male presenting with inflammatory colonic obstruction, secondary to acute gangrenous cholecystitis. He was successfully treated with a laparotomy, adhesiolysis and cholecystotomy and went on to make a good recovery. The case highlights the importance of having a high index of suspicion for acute gangrenous cholecystitis accompanied by inflammatory colonic obstruction when reviewing patients presenting with cholecystolithiasis and colonic obstruction in the presence of raised inflammatory markers, as well as having an early surgery