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1.
J Cutan Pathol ; 44(3): 279-282, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-27718529

RESUMO

Blepharochalasis is a rare disorder of unknown etiology defined by loose, atrophic periorbital skin following recurrent episodes of eyelid edema. Characteristic histopathology shows complete absence of elastic fibers. The current case progressed after multiple episodes of crying, which may be related to matrix metalloproteinase dysregulation. This case offers further insights into the possible pathogenesis of blepharochalasis, paving the way for more targeted, disease-modifying therapies.


Assuntos
Choro , Edema/etiologia , Edema/patologia , Doenças Palpebrais/etiologia , Doenças Palpebrais/patologia , Feminino , Humanos , Adulto Jovem
2.
Am J Dermatopathol ; 38(9): e137-9, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27541173

RESUMO

Metastatic melanoma is traditionally diagnosed using classic morphologic features in addition to immunohistochemical studies. The authors report a case of metastatic malignant melanoma where both morphology and immunohistochemistry were altered after treatment. This 51-year-old patient presented with metastatic melanoma to the brain and axilla. Initially, both metastases showed classic morphology and diffuse staining with the pan-melanoma antibody cocktail. This cocktail is a combination of 3 antibodies commonly used to diagnose melanocytic neoplasms: Melan-A (MART-1), tyrosinase, and HMB-45. In combination, the cocktail is highly sensitive for detecting melanocytic neoplasms and is commonly used to diagnose metastatic melanoma. Her tumor was positive for the BRAF 1799T>A (V600E) mutation, and she was treated with BRAF inhibitor therapy (vemurafenib). However, the axillary tumor recurred after treatment with vemurafenib. The recurrent tumor showed a markedly different morphology and complete loss of staining with the pan-melanoma antibody cocktail. This loss of staining accompanied by the change in morphology was an observation not previously documented after therapy with vemurafenib. This case demonstrates a potential pitfall in the diagnosis of metastatic or recurrent malignant melanoma.


Assuntos
Antineoplásicos/uso terapêutico , Indóis/uso terapêutico , Melanoma/tratamento farmacológico , Melanoma/secundário , Sulfonamidas/uso terapêutico , Biomarcadores Tumorais/análise , Neoplasias Encefálicas/tratamento farmacológico , Neoplasias Encefálicas/secundário , Feminino , Humanos , Imuno-Histoquímica , Metástase Linfática/patologia , Pessoa de Meia-Idade , Neoplasias Cutâneas , Vemurafenib , Melanoma Maligno Cutâneo
3.
Am J Dermatopathol ; 35(6): 676-8, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-23872874

RESUMO

Erythema ab igne (EAI) is a reticulated, erythematous, or hyperpigmented dermatosis that is caused by chronic exposure to infrared radiation. Chronic lesions of EAI have significant potential for malignant transformation. We report a case of poorly differentiated carcinoma arising within a patch of long-standing EAI on the lower extremity of a 92-year-old female. This case highlights the importance of recognizing EAI early and counseling patients to avoid further exposure to the offending infrared source to reduce the risk of malignant transformation. EAI has been associated with several different types of cutaneous neoplasms including the Merkel cell carcinoma, squamous cell carcinoma, and cutaneous marginal zone lymphoma.


Assuntos
Carcinoma/patologia , Diferenciação Celular , Eritema/patologia , Hiperpigmentação/patologia , Raios Infravermelhos/efeitos adversos , Neoplasias Induzidas por Radiação/patologia , Lesões Pré-Cancerosas/patologia , Neoplasias Cutâneas/patologia , Idoso de 80 Anos ou mais , Biópsia , Carcinoma/etiologia , Eritema/etiologia , Feminino , Humanos , Hiperpigmentação/etiologia , Neoplasias Induzidas por Radiação/etiologia , Lesões Pré-Cancerosas/etiologia , Valor Preditivo dos Testes , Fatores de Risco , Neoplasias Cutâneas/etiologia
4.
Cell Rep ; 42(8): 112906, 2023 08 29.
Artigo em Inglês | MEDLINE | ID: mdl-37540599

RESUMO

Hippocampal CA1 neurons generate single spikes and stereotyped bursts of spikes. However, it is unclear how individual neurons dynamically switch between these output modes and whether these two spiking outputs relay distinct information. We performed extracellular recordings in spatially navigating rats and cellular voltage imaging and optogenetics in awake mice. We found that spike bursts are preferentially linked to cellular and network theta rhythms (3-12 Hz) and encode an animal's position via theta phase precession, particularly as animals are entering a place field. In contrast, single spikes exhibit additional coupling to gamma rhythms (30-100 Hz), particularly as animals leave a place field. Biophysical modeling suggests that intracellular properties alone are sufficient to explain the observed input frequency-dependent spike coding. Thus, hippocampal neurons regulate the generation of bursts and single spikes according to frequency-specific network and intracellular dynamics, suggesting that these spiking modes perform distinct computations to support spatial behavior.


Assuntos
Ritmo Gama , Navegação Espacial , Ratos , Camundongos , Animais , Potenciais de Ação/fisiologia , Hipocampo/fisiologia , Neurônios/fisiologia , Ritmo Teta/fisiologia
5.
J Surg Oncol ; 104(1): 104-5, 2011 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-21360531

RESUMO

Perineural invasion is a feature associated with significantly poorer outcomes when present in cutaneous squamous cell carcinoma (CSCC). The incidence of this subset of CSCC continues to rise in the US, as does the confusion surrounding exactly how it should be managed. While management typically involves excision, considerable debate exists as to the appropriate use of adjuvant radiotherapy (ART) in addition to excision. This article reviews the current relevant evidence for the use of ART.


Assuntos
Carcinoma de Células Escamosas/radioterapia , Neoplasias do Sistema Nervoso Periférico/radioterapia , Neoplasias Cutâneas/radioterapia , Carcinoma de Células Escamosas/patologia , Humanos , Invasividade Neoplásica , Neoplasias do Sistema Nervoso Periférico/patologia , Radioterapia Adjuvante , Neoplasias Cutâneas/patologia
6.
Dermatol Surg ; 37(6): 744-9, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21605234

RESUMO

Skin cancers are common in organ transplant recipients (OTRs). In this review, we discuss the epidemiology of and risk factors for cutaneous neoplasms, particularly squamous cell carcinoma (SCC) in OTRs. The pathogenesis of SCC is reviewed, as well as the potential mechanisms for tumor progression and metastasis associated with two commonly used immunosuppressive medications: tacrolimus and cyclosporine. Finally, we discuss the mechanism of action and potential preventative use of sirolimus, a member of a newer class of immunosuppressants, the mammalian target of rapamycin inhibitors. The authors have indicated no significant interest with commercial supporters.


Assuntos
Carcinoma de Células Escamosas/prevenção & controle , Imunossupressores/uso terapêutico , Transplante de Órgãos , Sirolimo/uso terapêutico , Neoplasias Cutâneas/prevenção & controle , Carcinoma de Células Escamosas/etiologia , Humanos , Imunossupressores/efeitos adversos , Transplante de Órgãos/efeitos adversos , Sirolimo/efeitos adversos , Neoplasias Cutâneas/etiologia
7.
Am J Dermatopathol ; 33(6): 621-3, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21712693

RESUMO

BACKGROUND: Nummular eczema is a chronic inflammatory condition characterized by pruritus and histologically characterized by spongiosis. The etiology is unknown, and the lesions frequently arise spontaneously. Neuropeptides contribute to mediating and maintaining eczematous conditions. Previous research indicates that the number of immunoreactive nerve fibers is increased in pruritic skin lesions. OBJECTIVE: We sought to determine if the number of immunoreactive nerve fibers is increased in nummular eczema, as in other inflammatory pruritic conditions. METHODS: Protein gene product 9.5 (PGP 9.5) was assessed by immunohistochemistry in 22 biopsies of nummular eczema and was compared with immunohistochemical expression of 8 skin biopsies uninvolved by nummular eczema. RESULTS: Nerve fiber distribution using PGP 9.5 stain showed that there was significantly reduced PGP9.5 stain amount in the epidermis of patients with nummular eczema compared with their respective healthy control (P = 0.0054). However, no statistical difference was seen in the papillary dermis. CONCLUSION: Pruritus of nummular eczema is not associated with an increase of epidermal nerve fiber density and sprouting.


Assuntos
Eczema/patologia , Epiderme/patologia , Fibras Nervosas/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia , Eczema/metabolismo , Epiderme/inervação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fibras Nervosas/metabolismo , Estudos Retrospectivos , Ubiquitina Tiolesterase/metabolismo , Adulto Jovem
8.
Cutis ; 87(6): 278-80, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21838083

RESUMO

Epidermolytic hyperkeratosis is a rare congenital ichthyosis. Platelike osteoma cutis also is a rare diagnosis and is associated with abnormal ossification of cutaneous or subcutaneous tissue. A 17-month-old Hispanic girl presented with a plate of subcutaneous bone since birth as well as considerable scaling and hyperkeratosis centered around the joints. Histologic examination confirmed the diagnosis of both epidermolytic hyperkeratosis and osteoma cutis. Although there have been some cases of epidermolytic hyperkeratosis with other dermatologic conditions, we report a rare case of epidermolytic hyperkeratosis and platelike osteoma cutis.


Assuntos
Neoplasias Ósseas/diagnóstico , Hiperceratose Epidermolítica/diagnóstico , Osteoma/diagnóstico , Neoplasias Cutâneas/diagnóstico , Biópsia , Neoplasias Ósseas/patologia , Diagnóstico Diferencial , Feminino , Humanos , Hiperceratose Epidermolítica/patologia , Lactente , Osteoma/patologia , Neoplasias Cutâneas/patologia
9.
Cutis ; 87(2): 89-92, 2011 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21416776

RESUMO

A pseudolymphomatous reaction is an unusual immune response that can be caused by a tattoo, most commonly by red ink. We present the case of a 40-year-old woman with discolored and pruritic plaques in the areas of red ink tattooed on her right ankle that developed shortly after tattoo application. The patient had 2 other tattoos with red ink for more than 20 years without any disfigurement. Histopathology, immunohistochemistry, and polymerase chain reaction analysis from biopsy of the affected area showed polyclonal pseudolymphomatous and lichenoid reaction, confirming the diagnosis of pseudolymphoma secondary to a tattoo. Total excision of the lesion was performed.


Assuntos
Corantes/efeitos adversos , Erupções Liquenoides/etiologia , Pseudolinfoma/etiologia , Dermatopatias/etiologia , Tatuagem/efeitos adversos , Adulto , Biópsia , Feminino , Humanos , Erupções Liquenoides/patologia , Erupções Liquenoides/cirurgia , Pseudolinfoma/patologia , Pseudolinfoma/cirurgia , Dermatopatias/patologia , Dermatopatias/cirurgia
10.
Skinmed ; 9(4): 263-4, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21980715

RESUMO

A 53-year-old woman presented with a 20-year history of pruritic dermatitis on the groin, axillae, inframammary folds, posterior aspect of the neck, and popliteal fossae. She was referred to our clinic by an outside facility after results from a punch biopsy diagnosed Hailey-Hailey disease (HHD). The patient had previously attempted treatment with many traditional noninvasive options with no success. Topical treatment modalities included corticosteroids, immunomodulators, antifungals, retinoids, and antibiotic preparations. Intralesional corticosteroids, as well as botulinum toxin and carbon dioxide laser, were also unsuccessful. Failed systemic treatment modalities included antibiotics, antihistamines, prednisone, azathioprine, mycophenolate mofetil, acitretin, isotretinoin, adalimumab, and etanercept. Of note, cyclosporine was successful in clearing the cutaneous involvement in our patient, but elevation ofcreatinine and exacerbated hypertension precluded continued use. The decision was made to treat the patient by dermabrasion with sandpaper. The patient was prepped in a sterile fashion, and a field block with 1% lidocaine with epinephrine was performed. This was followed by abrasion down to the superficial dermis with 3M Sandblaster fine sandpaper (3M, St. Paul, MN) and hyfrecation between rounds ofdermabrasion. The treated areas were then covered with petrolatum and sterile gauze, and antibiotics and pain medication were prescribed. This treatment was initially performed on the patient's posterior aspect of the neck and later to the bilateral popliteal fossae and axillae. Three months post-treatment, desirable functional and cosmetic results of the treated areas had been achieved (Figure 1 and Figure 2). While no recurrence of clinically active HHD has been seen in the dermabraded areas of the neck and popliteal fossae, the patient continues to have active disease in the axillae despite sandpaper dermabrasion. To quantify our results, we performed two biopsies in the dermabraded sites of the popliteal fossae as healing occurred: a shave biopsy from an obviously active area, and a punch biopsy from a peripheral inactive border. The biopsy from the active area showed diffuse epidermal acantholysis similar to that seen in untreated HHD, while the healing periphery showed only scattered acantholytic areas and a sparse perivascular infiltrate-a marked improvement from the untreated areas.


Assuntos
Dermabrasão , Pênfigo Familiar Benigno/terapia , Feminino , Humanos , Pessoa de Meia-Idade
14.
Am J Dermatopathol ; 32(7): 713-5, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20700041

RESUMO

Atypical fibroxanthoma (AFX) is a lesion that typically occurs on actinically damaged skin of the head and neck. Histopathology of these neoplasms reveals atypical cells, which often demonstrate positive staining for immunohistochemical markers such as vimentin and CD10. In the case reported, keloidal collagen was discovered on histopathology. To our knowledge, only rare reports in the literature describe an AFX with similar histopathologic findings, suggesting that this is a distinctive presentation of AFX.


Assuntos
Colágeno Tipo I/biossíntese , Histiocitoma Fibroso Benigno/patologia , Pró-Colágeno/biossíntese , Neoplasias Cutâneas/patologia , Biomarcadores Tumorais/análise , Diabetes Mellitus , Orelha/patologia , Histiocitoma Fibroso Benigno/metabolismo , Histiocitoma Fibroso Benigno/cirurgia , Humanos , Hipertensão/complicações , Imuno-Histoquímica , Falência Renal Crônica/complicações , Masculino , Pessoa de Meia-Idade , Neprilisina/biossíntese , Diálise Renal , Neoplasias Cutâneas/metabolismo , Neoplasias Cutâneas/cirurgia
15.
Physiol Genomics ; 38(3): 372-85, 2009 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-19531578

RESUMO

Lasp1 is an actin-binding, signaling pathway-regulated phosphoprotein that is overexpressed in several cancers. siRNA knockdown in cell lines retards cell migration, suggesting the possibility that Lasp1 upregulation influences cancer metastasis. Herein, we utilized a recently developed gene knockout model to assess the role of Lasp1 in modulating nontransformed cell functions. Wound healing and tumor initiation progressed more rapidly in Lasp1(-/-) mice compared with Lasp1(+/+) controls. Embryonic fibroblasts (MEFs) derived from Lasp1(-/-) mice also migrated more rapidly in vitro. These MEFs characteristically possessed increased focal adhesion numbers and displayed more rapid attachment compared with wild-type MEFs. Differential microarray analyses revealed alterations in message expression for proteins implicated in cell migration, adhesion, and cytoskeletal organization. Notably, the focal adhesion protein, lipoma preferred partner (LPP), a zyxin family member and putative Lasp1 binding protein, was increased about twofold. Because LPP gene disruption reduces cell migration, we hypothesize that LPP plays a role in enhancing the migratory capacity of Lasp1(-/-) MEFs, perhaps by modifying the subcellular localization of other motility-associated proteins. The striking contrast in the functional effects of loss of Lasp1 in innate cells compared with cell lines reveals distinct differences in mechanisms of motility and attachment in these models.


Assuntos
Movimento Celular/fisiologia , Proteínas de Homeodomínio/fisiologia , Proteínas de Neoplasias/fisiologia , Neoplasias Experimentais/patologia , 9,10-Dimetil-1,2-benzantraceno , Animais , Western Blotting , Adesão Celular , Proliferação de Células , Células Cultivadas , Proteínas do Citoesqueleto/genética , Proteínas do Citoesqueleto/metabolismo , Feminino , Fibroblastos/citologia , Fibroblastos/metabolismo , Adesões Focais/metabolismo , Perfilação da Expressão Gênica , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Proteínas com Domínio LIM , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microscopia Confocal , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Neoplasias Experimentais/induzido quimicamente , Neoplasias Experimentais/genética , Análise de Sequência com Séries de Oligonucleotídeos , Interferência de RNA , Reação em Cadeia da Polimerase Via Transcriptase Reversa
16.
Antimicrob Agents Chemother ; 53(4): 1639-41, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19139291

RESUMO

Experience with anidulafungin against Candida krusei is limited. Immunosuppressed mice were injected with 1.3 x 10(7) to 1.5 x 10(7) CFU of C. krusei. Animals were treated with saline, 40 mg/kg fluconazole, 1 mg/kg amphotericin B, or 10 and 20 mg/kg anidulafungin for 5 days. Anidulafungin improved survival and significantly reduced the number of CFU/g in kidneys and serum beta-glucan levels.


Assuntos
Antifúngicos/uso terapêutico , Candida/isolamento & purificação , Candidíase/tratamento farmacológico , Equinocandinas/uso terapêutico , beta-Glucanas/sangue , Anidulafungina , Animais , Candidíase/sangue , Candidíase/microbiologia , Candidíase/mortalidade , Modelos Animais de Doenças , Rim/microbiologia , Masculino , Camundongos
17.
Am J Dermatopathol ; 31(6): 591-3, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19590413

RESUMO

Sarcoidosis is a systemic inflammatory disorder characterized histologically by noncaseating granulomas in affected organs. Cutaneous manifestations of the disease such as papules, nodules, plaques, and ulcerations occur in approximately 25% of the patients. Sarcoidosis can present with multiple different morphologies including annular, psoriasiform, ichthyosiform, morpheaform, and verrucous. In this study, we report a 30-year-old African American man with a history of spinal tuberculosis as a child and slowly enlarging verrucous nodules that appeared at the age of 5 years. After an extensive infectious disease evaluation, the diagnosis of verrucous sarcoidosis was established with the presence of noncaseating granulomas and a completely negative workup for infectious etiologies.


Assuntos
Sarcoidose/patologia , Dermatopatias/patologia , Tuberculose da Coluna Vertebral/complicações , Adulto , Criança , Humanos , Masculino , Sarcoidose/complicações , Dermatopatias/complicações , Verrugas/patologia
19.
Diagn Microbiol Infect Dis ; 62(4): 474-7, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18990524

RESUMO

Anidulafungin, a newer echinocandin with potent antifungal activity, was tested in an 8-laboratory M23-style quality control (QC) study to determine disk diffusion (DD) method ranges. Two lots of 2-microg disks (MAST and BD) and 3 lots of Mueller-Hinton agar (with 0.5 microg/mL methylene blue + 2% glucose) were used against 4 QC strains. Proposed ranges are Candida albicans ATCC 90028 at 24 to 39 mm (95.0% in range), Candida krusei ATCC 6258 at 20 to 35 mm (94.7%), Candida parapsilosis ATCC 22019 at 15 to 27 mm (95.5%), and Candida tropicalis ATCC 750 at 21 to 38 mm (94.6%); however, the ranges were very wide (13-18 mm), indicating between observer interpretive or reagent variation and compromised reproducibility. Challenge strains with elevated echinocandin MIC values (> or = 4 microg/mL, nonsusceptible) were tested (DD and MIC) and compared with isolates with reproducible anidulafungin MIC results at 2 microg/mL (susceptible). Discrimination between these nonsusceptible and susceptible (MIC, < or = 2 microg/mL) yeast populations was very acceptable, for example, 97.6% categoric agreement by DD method.


Assuntos
Antifúngicos/farmacologia , Candida/efeitos dos fármacos , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/normas , Equinocandinas/farmacologia , Anidulafungina , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/métodos , Testes de Sensibilidade Microbiana , Controle de Qualidade
20.
Diagn Microbiol Infect Dis ; 60(2): 221-4, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17976944

RESUMO

Surveillance data for methicillin-resistant Staphylococcus aureus (MRSA) and multidrug resistance (MDR) among MRSA were analyzed by patient location and age. MRSA rates were 55.7% among inpatients (53.9% MDR) and 48.7% among outpatients (30.7% MDR), with MDR rates increasing with age. These findings could impact the guidelines for the management and treatment of staphylococcal infections.


Assuntos
Resistência a Meticilina , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Fatores Etários , Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla , Humanos , Pacientes Internados , Testes de Sensibilidade Microbiana , Pacientes Ambulatoriais , Prevalência , Infecções Estafilocócicas/epidemiologia , Staphylococcus aureus/isolamento & purificação , Estados Unidos/epidemiologia
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