Early Upper Paleolithic cultural variability in the Southern Levant: New evidence from Nahal Rahaf 2 Rockshelter, Judean Desert, Israel.

The Levantine Early Upper Paleolithic (ca. 45-30 ka) has been a focus of research because of its unique position as a conduit of human, flora, and fauna species between Africa and Eurasia. Studies have mainly focused on the Early Ahmarian and Levantine Aurignacian, two entities, the former endemic and the latter foreign, which are considered to have coinhabited the region during that period. However, other cultural entities, such as the Atlitian in the Mediterranean region and the Arkov-Divshon in the arid regions of the southern Levant received less attention, and accordingly, suffer from broad definitions and chronological insecurity. These cultures hold potential insights regarding nuanced adaptations, reciprocal influences, and diachronic assimilation processes. The recently discovered site of Nahal Rahaf 2 Rockshelter in the Judean Desert provides integral information on one of these entities-the Arkov-Divshon. Two excavation seasons revealed a sequence of archaeological layers, with lithic assemblages in which laterally carinated items were prominent. Alongside rich faunal assemblages, other components of the material culture include perforated marine shells and bone tools, marking the first association of these elements with Arkov-Divshon and implying some degree of contact with the Mediterranean regions of the Levant. Good preservation of organic materials allowed radiocarbon dating of the human occupation at the site to ca. 37.5-34.0 ka cal BP, indicating chronological overlap with the Levantine Aurignacian, and possibly also with the latest phases of the Early Ahmarian. Thus, challenging the validity of the widely accepted 'Two Tradition' Model of the Levantine Upper Paleolithic. Lithic analyses suggest the use of one main reduction sequence and the primary production of bladelets from carinated items. Faunal remains suggest targeted hunting of ibex and gazelle. Botanical remains and sedimentary analyses suggest roughly similar environmental conditions, with a possible woodier environment in the surroundings of the site.

Preliminary observations on the Levantine Aurignacian sequence of Manot Cave: Cultural affiliations and regional perspectives.

A well-preserved sequence of Early Upper Paleolithic (EUP) occupations has been revealed in the past decade in Manot Cave, the studies of which shed light on the cultural dynamics and subsistence patterns and paleoenvironment. Most intriguing is the series of overlying Levantine Aurignacian occupation layers, exposed near the entrance to the cave. Area E is considered the inner part of the main activity area in Manot Cave. Remains of intact combustion features, as well as numerous flint artifacts and faunal remains, were found, indicating a high level of preservation. Within a 2.5 m sequence, nine distinct occupation layers were defined. The presence of characteristic flint and osseous industries alongside a rich mollusk assemblage led to the initial association of the sequence as a whole to the Levantine Aurignacian. However, as research advanced and variability in the material culture became apparent, it became clear that a division of the sequence into two phases, early and late, is required. A preliminary study of the assemblage variability implies distinct changes in human behavior between the two phases. Most prominently, these are indicated by a change in bladelet production method and morphology alongside an increase in the significance of the bladelet component within the flint assemblage, the disappearance of composite osseous industries, and a steep decrease in mollusk shell representation in the late occupation phase. Radiocarbon dating indicates a short time span between the two phases. The earlier phase defined as, Levantine Aurignacian, was ascribed an age range of 38-34 ka cal BP with a more constrained age range of 37-35 ka cal BP suggested based on Bayesian models. In the late phase, which is temporarily referred to as "post-Levantine Aurignacian," an age range of 36-33 ka cal BP is suggested.

Personal ornaments from Hayonim and Manot caves (Israel) hint at symbolic ties between the Levantine and the European Aurignacian.

Situated at the crossroads of Africa and Eurasia, the Levant is a crucial region for understanding the origins and spread of Upper Paleolithic (UP) traditions associated with the spread of modern humans. Of the two local Early Upper Paleolithic technocomplexes, the Ahmarian and the Levantine Aurignacian, the latter appears to be unique in the endemic UP sequence, exhibiting greater similarity to the West European 'classic' Aurignacian than to the local preceding and proceeding UP entities. Previous publications have mostly focused on the similarities between the two lithic industries and less on studies conducted on Levantine Aurignacian bone tools and ornaments. Here, we present an archaeozoological, technological and use-wear study of ornaments on animal teeth from the Levantine Aurignacian layers at Manot and Hayonim caves (the Galilee, Israel). The selection of taxa, the choice of teeth, the mode of modification, and the use-wear analysis exhibit clear similarities with the European Aurignacian. This, with the technology of the osseous raw material exploitation, the presence of antler simple-base points, and some lithic typotechnological features, suggest a link between the symbolic spheres of the Levantine and the European Aurignacian cultural entities. Such similarity also supports some contribution of European Aurignacians groups to the local cultural entities, intermingling with the local material culture features.

Cultural Dynamics in the Levantine Upper Paleolithic, ca. 40-33 ky BP: Insights Based on Recent Advances in the Study of the Levantine Aurignacian, the Arkov-Divshon, and the Atlitian.

The chrono-cultural sequence of the Levantine Upper Paleolithic went through several major revisions during approximately a century of focused research, each revision contributing to shedding light on the mosaic of cultural entities and the complex social and cultural dynamics composing the Levantine Upper Paleolithic. The current state of research suggests the co-inhabitance of two cultural groups: the Early Ahmarian and the Levantine Aurignacian. Two other cultural entities, the Arkov-Divshon and the Atlitian, are regarded as younger manifestations and were tentatively suggested to relate to the Levantine Aurignacian. This paper presents a research synthesis of two case studies: Manot Cave, located in western Galilee, Israel, and Nahal Rahaf 2 Rockshelter in the Judean Desert. The application of high-resolution excavation methods, alongside detailed documentation of the stratigraphy and site-formation processes and wide-scale radiocarbon-based absolute dating, marked these sites as ideal for chrono-cultural study through the analyses of flint industries. The results indicate a clear distinction between the Levantine Aurignacian and the Arkov-Divshon/Atlitian industries and a chronological overlap between the Arkov-Divshon, Levantine Aurignacian, and possibly with the Early Ahmarian. Subsequently, we suggest another revision of the currently accepted chrono-cultural model: not two, but at least three cultural entities co-inhabited the Levant at ca. 40-30 ky cal BP. This study further suggests an evolvement of the Atlitian flint industries from the Arkov-Divshon and stresses the foreign cultural features of the Levantine Aurignacian. These results were used to construct an updated model of migration and possible interaction patterns.

Long-term outcomes and patterns of recurrence in patients with thin melanoma and a negative sentinel lymph node biopsy: a single-center experience.

The majority of patients diagnosed with melanoma have thin melanomas (≤1 mm). Data on the rate and pattern of recurrence after a negative sentinel lymph node biopsy (SLNB) are sparse. We retrospectively searched our institutional database and retrieved the records of patients with thin melanomas who underwent an SLNB with negative results. We analyzed patterns of recurrence, time to recurrence, and mode of diagnosis. Thirteen of the 198 patients with thin melanomas and negative SLNB results had tumor recurrence (6.5%): two local in transit (15.4%), three regional (21.3%), and eight distant (61.5%). Distant recurrences tended to occur later than local or regional ones [median disease-free survival = 50 months (95% confidence interval: 36.1-63.9) vs. 34 and 15 months (95% confidence interval: 5.4-24.6), P = 0.005, respectively]. The percentage of patients with tumor thickness ≥0.8 mm was higher among those who sustained recurrence (84.6 vs. 64.9% for no recurrence, P = 0.04). The majority of patients with recurrence were not being followed up when diagnosed (69%), and they are presented because of clinical symptoms. Patients with recurrence had lower survival compared with those without recurrence (median: 118 months vs. ongoing survival, P < 0.001, respectively). Melanoma recurrence in patients with thin melanomas and negative SLNBs is rare, tends to be distant, and negatively affects prognosis. Recurrence tends to occur in patients with melanoma thickness ≥0.8 mm. Further studies are needed to identify patients with high recurrence risk and determine optimal follow-up protocols.

Cervidae antlers exploited to manufacture prehistoric tools and hunting implements as a reliable source of ancient DNA.

Antler is one of the primary animal raw materials exploited for technical purposes by the hunter-gatherer groups of the Eurasian Upper Palaeolithic (UP) all over the ecological range of deers, and beyond. It was exhaustively employed to produce one of the most critical tools for the survival of the UP societies: hunting weapons. However, antler implements can be made from diverse deer taxa, with different ecological requirements and ethological behaviours. Identifying the antler's origin at a taxonomic level is thus essential in improving our knowledge of humans' functional, practical and symbolic choices, as well as the human-animal interface during Prehistoric times. Nevertheless, palaeogenetics analyses have focused mainly on bone and teeth, with genetic studies of antler generally focused on modern deer conservation. Here we present the results of the first whole mitochondrial genome ancient DNA (aDNA) analysis by means of in-solution hybridisation capture of antlers from pre-Holocene archaeological contexts. We analysed a set of 50 Palaeolithic and Neolithic (c. 34-8ka) antler and osseous objects from South-Western Europe, Central Europe, South-Western Asia and the Caucasus. We successfully obtained aDNA, allowing us to identify the exploited taxa and demonstrate the archaeological relevance of those finds. Moreover, as most of the antlers were sampled using a minimally-invasive method, further analyses (morphometric, technical, genetic, radiometric and more) remain possible on these objects.

Late Acheulian Jaljulia - Early human occupations in the paleo-landscape of the central coastal plain of Israel.

The Lower Paleolithic Late Acheulian in the Levant marks a fascinating chapter in human cultural and biological evolution. Nevertheless, many aspects of the Late Acheulian are still undeciphered, hindered by the complex nature of each site on the one hand, a scarcity of wide, multidisciplinary studies on the other, and by difficulties in obtaining absolute chronology for this timeframe. Therefore, subjects such as human subsistence strategies and modes of adaptation, regional diversity, and the possible existence and nature of interactions between hominin groups are largely understudied. The discovery and study of Jaljulia, a large-scale Late Acheulian site at the central Coastal Plain, Israel, add valuable insights to the research of this chapter in human history. Considered to represent recurrent occupations at a favored, water and flint-rich setting, the site has provided extensive lithic assemblages obtained from several localities. Absolute chronology places the human activity on-site at roughly 500-300 ky (and possibly even later), which is suggested to be divided into several main occupation phases. Geomorphological and sedimentological analyses show a change in environmental conditions, from aeolian sand deposition and overlying Hamra soil during the Middle Pleistocene to high energy fluvial regime which transported large gravels in a north-south paleo-channel. Wetland environments, correlating to the human activity on site, developed later due to higher sea levels and a coastline shifts to the eastward, which caused a blockage of the Yarkon stream corridor to the sea by marine sand. In this paper we present results of the study of the site, including geomorphological formation and post-depositional processes, absolute chronology, lithic and faunal analyses. The site's extensive lithic assemblages are currently under study and future investigations are expected to shed more light on the technological nature of Late Acheulian Jaljulia.