Sulfonyl Molecules Induced Oriented Lithium Deposition for Long-Term Lithium Metal Batteries.

Dendrites growth and unstable interfacial Li+ transport hinder the practical application of lithium metal batteries (LMBs). Herein, we report an active layer of 2,4,6-trihydroxy benzene sulfonyl fluorine on copper substrate that induces oriented Li+ deposition and generates highly crystalline solid-electrolyte interphase (SEI) to achieve high-performance LMBs. The lithiophilic -SO2 - groups of highly crystalline SEI accept the rapidly transported Li+ ions and form a dense inner layer of LiF and Li3 N, which regulate Li+ plating morphology along the (110) crystal surface toward dendrite-free Li anode. Thus, Li||Cu cells with lithiophilic SEI achieve an average deposition efficiency of 99.8 % after 700 cycles, and Li||Li cells operate well for 1100 h. Besides, Li||LiNi0.8 Co0.1 Mn0.1 O2 cells with modified SEI exhibit a capacity retention that is 14 times than that of conventional SEI. Even at -60 °C, Li||Cu cells reach stable deposition efficiency of 83.2 % after 100 cycles.

Metallo-beta-lactamase CphA evolving into more efficient hydrolases through gene mutation is a novel pathway for the resistance of super bacteria.

The evolution of metallo-beta-lactamase CphA in discontinuous gradient concentration of imipenem was investigated in this work. The results suggested that single-base mutations K218R, K249T, K249M, Q253H, and a frameshift mutation M1 were observed. Compared with wild type, the minimum inhibitory concentration (MICs) of K249T, K249M, and M1 increased by at least 128 times and that of K218R increased by 64 times. And the catalytic efficiency increased by 312% and 653%, respectively. It is speculated from the details of the structural changes revealed by molecular dynamics simulations that the carbon skeleton migration caused by the outward motion of the loop 3 in the mutant may have significantly increased the cavity volume of the binding pocket, which is more conducive to the entry and expulsion of imipenem and its hydrolytic product. And the conformational change of the TDRAGGN (71-77) is located at the bottom of the binding pocket from order α-helix to disorder random coil enabled the binding pocket to be more conducive to accommodate and hold the imipenem respectively. All these indicated that during the repeated drug resistance, the wild-type achieved gene mutations and conformational change and evolved to the mutant enzymes with a more delicate structure and stronger hydrolysis ability. KEY POINTS: • The mutation and evolution of CphA under the selective pressure of imipenem. • The CphA evolved to the mutants with stronger hydrolysis capacity. • A novel pathway for the resistance of super bacteria.

Nickel Nitride Particles Supported on 2D Activated Graphene-Black Phosphorus Heterostructure: An Efficient Electrocatalyst for the Oxygen Evolution Reaction.

Hydrogen is regarded as the most promising green clean energy in the 21st century. Developing the highly efficient and low-cost electrocatalysts for oxygen evolution reaction (OER) is of great concern for the hydrogen industry. In the water electrolyzed reaction, the overpotential and the kinetics are the main hurdles for OER. Therefore, an efficient and durable oxygen evolution reaction electrocatalyst is required. In this study, an activated graphene (AG)-black phosphorus (BP) nanosheets hybrid is fabricated for supporting Ni3 N particles (Ni3 N/BP-AG) in the application of OER. The Ni3 N particles are combined with the BP-AG heterostructure via facile mechanical ball milling under argon protection. The synthesized Ni3 N/BP-AG shows excellent catalytic performance toward the OER, demanding the overpotential of 233 mV for a current density of 10 mA cm-2 with a Tafel slope of 42 mV dec-1 . The Ni3 N/BP-AG catalysts also show remarkable stability with a retention rate of the current density of about 86.4% after measuring for 10 000 s in potentiostatic mode.

Boron-Doped Diamond for Hydroxyl Radical and Sulfate Radical Anion Electrogeneration, Transformation, and Voltage-Free Sustainable Oxidation.

Boron-doped diamond-based electrochemical advanced oxidation processes (BDD-EAOPs) have attracted much attention. However, few systematic studies concerning the radical mechanism in BDD-EAOPs have been published. In situ electron paramagnetic resonance spectrometry is used to confirm that SO4•- is directly electrogenerated from SO42- . Then, excess SO4•- dimerizes to form S2 O82- and accumulates in the BDD-EAOP system. But no S2 O82- accumulates at pH = 10 owing to the rapid transformation of SO4•- and S2 O82- . Above the overpotential of water oxidation, • OH is electrogenerated and cooperated with SO4•- . In the power-off phase, the accumulated S2 O82- can be reactivated to SO4•- via specific degradation intermediates to achieve sustainable degradation. Di-n-butyl phthalate (DnBP), a typical endocrine disruptor, is selected as a model contaminant. Surprisingly, 99.8% of DnBP (initial concentration of 1 mg L-1 ) is removed, using an intermittent power supply strategy with a periodic 10 min power-on phase at a duty ratio of 1:2, reducing the electrical energy consumption (1.8 kWh m-3 ) by more than 30% compared with continuous power supply consumption. These radical electrogeneration transformation mechanisms reveal an important new strategy for sustainable oxidation, especially for in situ water restoration, and are expected to provide a theoretical basis for BDD applications.

Probing the interaction of l-captopril with metallo-ß-lactamase CcrA by fluorescence spectra and molecular dynamic simulation.

Both the molecular recognition and interaction of metallo-ß-lactamase CcrA with l-captopril were studied by the combined use of fluorescence spectra and molecular dynamic simulation. The results showed that the binding constant was 8.89 × 104  L mol-1 at 296 K. Both Zn1 and Zn2 displayed tetrahedral coordination geometries in the CcrA-Lcap complex, the S atom in l-captopril displaced the nucleophilic hydroxide in apo CcrA and occupied the fourth coordination site for each ion, resulting in a competitively inhibited CcrA enzyme. Strong electrostatic interaction between the two zinc ions in CcrA and negatively charged l-captopril provided the main driving force for the binding affinity. Through a partly structural transformation from ß-sheet to random coil, loop 1 (residues 24-34) completely opened the binding pocket of CcrA to allow an induced fit of the newly introduced ligand. This study may provide some valuable information for designing and developing a more tightly binding inhibitor to resist superbugs.

Spatial-temporal evolution and intrinsic drivers of compound drought and heatwave events in Mainland China.

Given the devastating effects and potential rising trends of compound drought and heatwave (CDH) events under the specter of global warming, this study embarks on a comprehensive examination of their spatial and temporal evolution, as well as the intrinsic drivers. This study identified CDH events based on the non-stationary standardized precipitation evapotranspiration index (NSPEI) and the relative threshold method. The study also quantified the spatial and temporal patterns of frequency, intensity, and duration of CDH events across different climatic sub-regions, quantifying the contribution of drought-heatwave interdependence to these events and assessing the impact of single extreme climate events on their proliferation. The study yielded several key findings: 1) The frequency, intensity, and duration of CDH events exhibited high spatial heterogeneity and a significant increasing trend over the study period. 2) A notable positive interdependence was observed between the occurrences of droughts and heatwaves, significantly contributing to the rise in CDH events. 3) Droughts exacerbated the intensity and duration of CDH events compared to heatwaves under non-drought conditions (NDCH). 4) The spatial distribution characteristics and the change indices of heatwaves and CDH events were strikingly similar, indicating a consistent evolution. Notably, the increase in heatwaves had a more pronounced influence on the escalation of CDH events compared to droughts. 5) The West Pacific Subtropical High (WPSH) and the South Asian High (SAH) have had significant impacts on CDH events in mainland China. This research provides vital insights into the dynamics of CDH events, emphasizing their growing frequency and severity in the context of climate change. It offers a crucial perspective for policymakers and disaster management authorities in developing targeted strategies for climate adaptation and mitigation.

Effect of pyrolysis temperature on the binding characteristics of DOM derived from livestock manure biochar with Cu(II).

Biochar-derived dissolved organic matter (BDOM) has the potential to influence the environmental application of biochar and the behavior of heavy metals. In this study, the binding properties of BDOM derived from livestock manure biochar at different pyrolysis temperatures with Cu(II) were investigated based on a multi-analytical approach. The results showed that the DOC concentration, aromatics, and humification degree of BDOM were higher in the process of low pyrolysis of biochar. The pyrolysis temperature changed the composition of BDOM functional groups, which affected the binding mechanism of BDOM-Cu(II). Briefly, humic-like and protein-like substances dominated BDOM-Cu(II) binding at low and high pyrolysis temperatures, respectively. The higher binding capacity for Cu(II) was exhibited by BDOM derived from the lower pyrolysis temperature, due to the carboxyl as the main binding site in humic acid had high content and binding ability at low-temperature. The amide in proteins only participated in the BDOM-Cu(II) binding at high pyrolysis temperature, and polysaccharides also played an important role in the binding process. Moreover, the biochar underwent the secondary reaction at certain high temperatures, which led to condensation reaction of the aromatic structure and the conversion of large molecules into small molecules, affecting the BDOM-Cu(II) binding sites.

Identification of a new HIV-1 circulating recombinant form (CRF159_01103) derived from CRF103_01B and CRF01_AE in Hebei Province, China.

Recombinant HIV-1 genomes identified in three or more epidemiological unrelated individuals are defined as circulating recombinant forms (CRFs). CRFs can further recombine with other pure subtypes or recombinants to produce secondary recombinants. In this study, a new HIV-1 intersubtype CRF, designated CRF159_01103, isolated from three men who have sex with men with no epidemiological linkage, was identified in Baoding city, Hebei Province, China. CRF159_01103 was derived from CRF103_01B and CRF01_AE. Bayesian molecular clock analysis was performed on the CRF01-AE and CRF103_01B regions of CRF159_01103. The time of origin of CRF159_01103 was predicted to be 2018-2019, indicating that it is a recent recombinant virus. The emergence of CRF159_01103 has increased the complexity of the HIV-1 epidemic in Hebei Province.

HIV-1 env gene mutations outside the targeting probe affects IPDA efficiency.

The intact proviral DNA assay (IPDA) based on droplet digital PCR was developed to identify intact proviral DNA and quantify HIV-1 latency reservoirs in patients infected with HIV-1. However, the genetic characteristics of different HIV-1 subtypes are non-consistent due to their high mutation and recombination rates. Here, we identified that the IPDA based on the sequences features of an HIV-1 subtype could not effectively detect different HIV-1 subtypes due to the high diversity of HIV-1. Furthermore, we demonstrated that mutations in env gene outside the probe binding site affect the detection efficiency of IPDA. Since mutations in env gene outside the probe binding site may also lead to the formation of stop codons, thereby preventing the formation of viruses and ultimately overestimating the number of HIV-1 latency reservoirs, it is important to address the effect of mutations on the IPDA.

Treatment with Antiviral Drugs Will Significantly Inhibit the HIV-1 RNA POL Gene Expression and Viral Load in AIDS Patients.

Objective: This study is to investigate the difference in HIV-1 RNA pol gene expression in AIDS patients before and after antiviral treatment and its effect on the expression level of CD4+/CD8+ T cells in peripheral blood. Methods: The participants included 200 AIDS patients who had undergone antiviral medication, and the quantity of HIV-1 RNA pol gene was determined using nested polymerase chain reaction (nPCR). The levels of CD3+, CD4+, and CD8+ T lymphocytes in peripheral blood were measured by flow cytometry before and after therapy. The receiver operating characteristics (ROC) curve was used to assess the impact of HIV-1 RNA pol gene expression and the CD4+/CD8+ ratio on the prognosis of AIDS patients. Results: After three months of therapy, the levels of HIV-1 RNA and viral load in the patients showed a drastic decline, while the levels of CD4+/CD8+ were markedly elevated (P < 0.05). Logistic analysis revealed that patients' viral loads were positively correlated with HIV-1 RNA and negatively correlated with CD4+/CD8+ (P < 0.05). The alanine aminotransferase (ALT), white blood cell (WBC) count, Serum creatinine (Cr), total cholesterol (TC), triglyceride (TG), and platelet (PLT) levels significantly increased following a 24-month therapy, while no significant changes were observed in the level of aspartate aminotransferase (AST), red blood cell (RBC), and neutrophil (NEU) (%). (P > 0.05). Conclusion: Antiviral drugs significantly inhibit the HIV-1 RNA POL gene expression and viral load in AIDS patients but upregulate the expression level of CD4+/CD8+ T cells in peripheral blood.

Nano-semiconductor material stabilized Zn metal anode for long-life aqueous Zn-ion batteries.

The advantages of aqueous Zn-ion batteries lie in the affordability and environmental friendliness. Nonetheless, the use of aqueous Zn-ion batteries is severely hindered by key issues such dendrite formation and side reactions in Zn metal anodes. It is able to works well so as to create a stable interface layer, which controls the development of dendrites and adverse reactions. In this study, it is recommended that the coating formed by nano-semiconductor material graphitic carbon nitride (g-C3N4) should be applied to the surface of Zn metal to evenly disperse the electric field, as well as inhibit the development of tip effect, thus preventing Zn dendrite growth. Zn deposition occurs quickly and steadily as a result of Zn2+ ions being adsorbed and the Zn2+ ion flow being reallocated by the zincophilicity of N atoms in the coating. The Zn symmetrical battery can be stable cycled for 1,000 h at a current density of 0.5 mA cm-2, with its overall areal capacity of 0.5mAh cm-2, which is attributed to these benefits of the coating. It can be stable circulated for 500 h at a high current density of 5 mA cm-2, with its total areal capacity of 1mAh cm-2. The completely constructed Zn-g-C3N4//V2O5 according exhibits exceptional long-termcycle stability. Under the current density of 2 A/g, the initial capacity is 312.3 mAh g-1, which can cycle be stable circulated for 1,000 cycles.Under the high current density of 5 A/g, the whole battery's capacity keeping holdingrate is 70% after 2000 cycles, and the coulomb efficiency (CE) is extremely near to 100%.

Repurposing of spent lithium-ion battery separator as a green reductant for efficiently refining the cathode metals.

Developing green and high-efficient pyrometallurgy processes to recycle precious metals from spent lithium-ion batteries (LIBs) is of great importance for resource sustainability and environmental protection. Herein, a novel reduction roasting approach relying on spent LIB separator to refine the spent cathode is proposed. The efficiency of repurposing separator as a reductant for roasting the spent LiCoO2 cathode and the underlying mechanisms were investigated. After the separator-mediated roasting at 500 °C for 2 h, Li+ leaching efficiency of the cathode reached 93.2 %, >2.6 times higher than those after roasting without reductant (25.2 %) or with benchmark reductant graphite (26.1 %). Under the separator-added roasting condition, the cathode was converted to the desired products, CoO and Li2CO3. Based on the analysis of in-situ reaction using thermogravimetric/differential scanning calorimetry and pyrolysis gas species identification, the separator-mediated reduction roasting of cathode was composed of two stages, i.e., reducing gas generation due to separator pyrolysis, followed by the reducing gas mediated LiCoO2 reduction. During the process, the generated C2H4 and CO dominated the reduction. The use of co-existing separator to recover precious metals from spent LIBs is an effective and sustainable strategy to maximize the utilization of spent LIBs.

Modification of fluorescence staining method for small-sized microplastic quantification: Focus on the interference exclusion and exposure time optimization.

Microplastics are an emerging pollutant of global concern, and fluorescence staining as an efficient method for small-sized microplastic qualification often undergoes the serious interference from external environments. The key steps affecting the accuracy of fluorescent staining and the corresponding quality assurance measures were rarely known. Therefore, this study took the Nile Red/DAPI co-staining method as an example to explore the key factors affecting its accuracy and effective measures to avoid interference. High background microplastic contamination in typical lab waters (up to 1115 MP/L), glass fiber filter membrane and glassware were identified as dominant factors affecting microplastic quantification. The background microplastics in lab waters mainly originated from the process of water production and storage. A simple filtration process removed 99% of the background microplastic in the lab waters. After burning at 500 °C for 1 h, the microplastic contamination in the filter membrane and glassware was completely eliminated. H2O2 pretreatment and exposure time caused erroneous microplastic size assessment, and were suggested to be set at 48 h and 10 ms, respectively. During the extraction process, the residue in beakers reached ~ 20% and > 50% for 5 µm and 20 µm sized microplastics, respectively, greatly contributing to the microplastic loss. The comprehensive modified measures caused microplastic concentrations in the three typical samples detected by Nile Red/DAPI co-staining method to decrease by 65.7 - 92.2% and to approach the micro-Raman results. This study clarified the reasons for interfering with quantitative microplastics by fluorescent staining and the effective measures to avoid interference, which were conducive to improving the accuracy of quantitative methods of microplastics.

Li3Bi/Li2O layer with uniform built-in electric field distribution for dendrite free lithium metal batteries.

Lithium metal batteries have garnered significant attention as a promising energy storage technology, offering high energy density and potential applications across various industries. However, the formation of lithium dendrites during battery cycling poses a considerable challenge, leading to performance degradation and safety hazards. This study aims to address this issue by investigating the effectiveness of a protective layer on the lithium metal surface in inhibiting dendrite growth. The hypothesis is that continuous lithium consumption during battery cycling is a primary contributor to dendrite formation. To test this hypothesis, a protective layer of Li3Bi/Li2O was applied to the lithium foil through immersion in a BiN3O9 solution. Experimental techniques including kelvin probe force microscopy (KPFM) and density functional theory (DFT) calculations were employed to analyze the structural and electronic properties of the Li3Bi/Li2O layer. The findings demonstrate successful doping of Bi into the Li coating, forming Bi-Bi and Bi-O bonds. KPFM measurements reveal a higher work function of Li3Bi/Li2O, indicating its potential as an effective protective layer. DFT calculations further support this observation by revealing a greater adsorption energy of lithium on the Li3Bi/Li2O layer compared to the bulk material. Charge density analysis suggests that the adsorption of Li atoms onto the Li3Bi/Li2O layer induces a redistribution of charge, resulting in increased electron availability on the surface and preventing electrode-electrolyte contact. This study provides insights into the role of the Li3Bi/Li2O protective layer in inhibiting dendrite growth in lithium metal batteries. By mitigating dendrite formation, the protective layer holds promise for enhancing battery performance and longevity. These findings contribute to the development of strategies for improving the stability and reliability of lithium metal batteries, facilitating their wider adoption in energy storage applications.

High-level production of nervonic acid in the oleaginous yeast Yarrowia lipolytica by systematic metabolic engineering.

Nervonic acid benefits the treatment of neurological diseases and the health of brain. In this study, we employed the oleaginous yeast Yarrowia lipolytica to overproduce nervonic acid oil by systematic metabolic engineering. First, the production of nervonic acid was dramatically improved by iterative expression of the genes ecoding ß-ketoacyl-CoA synthase CgKCS, fatty acid elongase gELOVL6 and desaturase MaOLE2. Second, the biosynthesis of both nervonic acid and lipids were further enhanced by expression of glycerol-3-phosphate acyltransferases and diacylglycerol acyltransferases from Malania oleifera in endoplasmic reticulum (ER). Third, overexpression of a newly identified ER structure regulator gene YlINO2 led to a 39.3% increase in lipid production. Fourth, disruption of the AMP-activated S/T protein kinase gene SNF1 increased the ratio of nervonic acid to lignoceric acid by 61.6%. Next, pilot-scale fermentation using the strain YLNA9 exhibited a lipid titer of 96.7 g/L and a nervonic acid titer of 17.3 g/L (17.9% of total fatty acids), the highest reported titer to date. Finally, a proof-of-concept purification and separation of nervonic acid were performed and the purity of it reached 98.7%. This study suggested that oleaginous yeasts are attractive hosts for the cost-efficient production of nervonic acid and possibly other very long-chain fatty acids (VLCFAs).

Mutation and evolution of metallo-beta-lactamase CphA under the selective pressure of biapenem continuous concentration gradient.

One of the resistance mechanisms of superbugs is to hydrolyze antibiotics by producing metallo-ß-lactamases (MßLs). To verify how MßLs evolved to increase in activity in response to various ß-lactam antibiotics, the mutation and evolution of CphA from Aeromonas hydrophila (Zn2+-dependent MßL) was investigated in a medium with a continuous biapenem (BIA) concentration gradient. The results showed that a single-base mutation M1 and two frameshift mutations M3 and M4 were observed. Furthermore, a nonsense mutation M2 was observed. Compared with wild-type (WT), the minimum inhibitory concentrations (MICs) of the M3 and M4 increased by more than 128 times, and the catalytic efficiency of BIA by the M3 and M4 increased by 752% and 376% respectively. In the mutants, the carbon skeleton migration caused by the outward motion of the loop3 near the entrance of the binding pocket increased the cavity volume of the binding pocket and was more conducive to the entry and expulsion of BIA and its hydrolytic product in the binding pocket. The conformational change effect originated from mutations is transmitted to the binding pocket through the interactions between the side chain amino acid residues of the C-terminal and those of the loop3, thus affecting the binding and hydrolysis capability of the mutants to BIA in the binding pocket. All these indicated that during the repeated drug-endurance and -resistance, the CphA completed its mutation and conformational change and evolved to the mutants with a more delicate structure and stronger hydrolysis ability by a genetic mutation.

Identification of Three Novel HIV-1 Second-Generation Recombinant Forms (CRF01_AE/CRF07_BC) Among Men Who Have Sex with Men in Baoding, Hebei, China.

The number of the second-generation recombinants originated from these two subtypes is significantly increasing due to co-circulating of CRF01_AE and CRF07_BC in China, especially among men who have sex with men (MSM). In this study, we reported three new unique recombinant forms (URFs) among MSM in Baoding, China. Phylogenetic and recombinant analyses based on the near full-length genome revealed these three URFs were the second-generation recombinant strains originated from CRF01_AE and CRF07_BC. Nowadays, the MSM has become a main route that causes the viral recombination. Therefore, the further epidemiological surveillance should be conducted in the MSM population to strengthen our knowledge of HIV-1 evolution and genetic diversity.

Roles of microplastic-derived dissolved organic matter on the photodegradation of organic micropollutants.

Microplastic-derived dissolved organic matter (MP-DOM) is ubiquitous in water environment and exhibits photosensitivity. However, little is known about the effects of MP-DOM on the photodegradation of organic micropollutants in natural water. In this study, we investigated the effect of MP-DOM derived from two typical plastics, i.e., polystyrene (PS), and polyethylene (PE), on the photodegradation of a typical organic micropollutants sulfamethoxazole (SMX) in a simulative natural water system. MP-DOM exerted a significant inhibition on the SMX photodegradation, mainly attributed to the direct photolysis inhibition of SMX caused by the inner filter effect and the complexation effect. Despite the enhanced reactive oxygen species (ROS) generation with the increase of their steady-state concentration by 41.1 - 160.7 %, PS-DOM exhibited high oxidation resistance, causing an inhibition on the photodegradation of SMX probably through transferring electrons to the SMX intermediates. This study helps to deepen the understanding of microplastic photochemical behavior in natural water.

Identification of a New HIV-1 Circulating Recombinant Form CRF112_01B Strain in Baoding City, Hebei Province, China.

BACKGROUND: A large number of HIV-1 recombinants that originated from CRF01_AE and B strains are constantly emerging in men who have sex with men populations in China and deserve more attention and further monitoring. OBJECTIVE: To analyze the near-full-length genome structure and recombination characteristics of a new HIV-1 strain (BD226AJ) detected in Baoding City and determine its subtype. CASE REPRESENTATION: Viral RNA was extracted from a blood sample collected from an infected individual and reverse transcribed to cDNA. Two overlapping segments of the HIV-1 genome were amplified using a near-endpoint dilution method and sequenced. Recombinant breakpoints were determined using RIP, jpHMM, and SimPlot 3.5.1 software. MEGA v6.0 was used to construct a neighbor-joining phylogenetic tree to determine the homology relationships of this strain. RESULTS AND DISCUSSION: We obtained 8830 nucleotides (nt) of the HIV-1 genome sequence by amplification and sequencing, and four recombinant fragments were identified by recombination analysis, namely CRF01_AE (HXB2, 823-4224 nt), subtype B (HXB2, 4225-5991 nt), CRF01_AE (HXB2, 5992-9295 nt), and subtype B (HXB2, 9296-9406 nt). The BLAST results showed that 96% of the sequence was similar to CRF112_01B. The jpHMM results confirmed that BD226AJ was the CRF112_01B strain. CONCLUSION: Our results confirm the first epidemic of CRF112_01B in Hebei Province. This finding suggests that HIV-1 CRF112_01B may have been introduced into Hebei by men who have sex with men and indicates that the epidemic trend of this strain should be closely monitored.

Prevalence of resistance mutations associated with integrase inhibitors in therapy-naive HIV-positive patients in Baoding, Hebei province, China.

Antiretroviral therapy (ART) regimens containing integrase strand transfer inhibitors (INSTIs) are the recommended treatment for human immunodeficiency virus type 1 (HIV-1)-infected patients in the most recent guidelines in China. In this study, we investigated INSTI resistance mutations in newly diagnosed therapy-naive HIV-positive patients in Baoding City, Hebei Province (China) to provide guidance for implementing routine INSTI-associated HIV-1 genotypic resistance testing. Plasma samples were collected from HIV-1-infected patients without treatment at Baoding People's Hospital from January 2020 to December 2021. The part of HIV-1 pol gene encoding integrase was amplified, sequenced, and analyzed for INSTI resistance. Clinical data including demographic data, CD4+ T cell counts, HIV-RNA loads, and resistance mutations were collected. Treatment-naïve HIV-1 patients (n = 131) were enrolled. We identified ten genotypes, and the predominant genotype was CRF01_AE in 67 patients (51.15%), CRF07_ BC in 39 patients (29.77%), subtype B in 11 patients (8.40%), and other subtypes (CRF68_01B, 3.82%; CRF55_01B, 1.53%, CRF80_0107, 1.53%; URFs 1.53%; and CRF103_01B, CRF59_01B, and CRF65_cpx, 1.4% each). Four major (E138A, R263k, G140S, and S147G) and three accessory (H51Y, Q146QL, and S153F) INSTI-resistance mutations were observed (genotype CRF01_AE, three patients; genotype B, one patient; and genotype CRF07_BC, one patient), resulting in different degrees of resistance to the following five INSTIs: raltegravir, elvitegravir, dolutegravir, bictegravir, and cabotegravir. The overall resistance rate was 3.82% (5/131). All INSTI-resistant strains were cross-resistant. The primary INSTI drug resistance rate among newly diagnosed HIV-infected patients in Baoding was low, but monitoring and research on HIV INSTI resistance should be strengthened in Baoding because INSTI-based regimen prescriptions are anticipated to increase in the near future.