Exposure to prenatal excess or imbalanced micronutrients leads to long-term perturbations in one-carbon metabolism, trimethylamine-N-oxide and DNA methylation in Wistar rat offspring.

Prenatal multivitamins, including folic acid, are commonly consumed in excess, whereas choline, an essential nutrient and an important source of labile methyl groups, is underconsumed. Here, we characterized profiles of one-carbon metabolism and related pathways and patterns of DNA methylation in offspring exposed to excess or imbalanced micronutrients prenatally. Pregnant Wistar rats were fed either recommended 1× vitamins (RV), high 10× vitamins (HV), high 10× folic acid with recommended choline (HFolRC), or high 10× folic acid with no choline (HFolNC). Offspring were weaned to a high-fat diet for 12 weeks. Circulating metabolites were analyzed with a focus on the hypothalamus, an area known to be under epigenetic regulation. HV, HFolRC, and HFolNC males had higher body weight (BW) and lower plasma choline and methionine consistent with lower hypothalamic S-adenosylmethionine (SAM):S-adenosylhomocysteine (SAH) and global DNA methylation compared with RV. HV and HFolNC females had higher BW and lower plasma 5-methyltetrahydrofolate and methionine consistent with lower hypothalamic global DNA methylation compared with RV. Plasma dimethylglycine (DMG) and methionine were higher as with hypothalamic SAM:SAH and global DNA methylation in HFolRC females without changes in BW compared with RV. Plasma trimethylamine and trimethylamine-N-oxide were higher in males but lower in females from HFolRC compared with RV. Network modeling revealed a link between the folate-dependent pathway and SAH, with most connections through DMG. Final BW was negatively correlated with choline, DMG, and global DNA methylation. In conclusion, prenatal intake of excess or imbalanced micronutrients induces distinct metabolic and epigenetic perturbations in offspring that reflect long-term nutritional programming of health.

Beyond the Coagulation Cascade: Vitamin K and Its Multifaceted Impact on Human and Domesticated Animal Health.

Vitamin K (VK) is an essential micronutrient impacting many systems in the body. This lipid-soluble vitamin is found in various plant and animal products and is absorbed via the lymphatic system. This biomolecule's importance to human health includes but is not limited to its promotion of brain, cardiovascular, bone, and immune functions. These biological properties are also necessary for maintaining domesticated animal health. The synergistic impact of both VK and vitamin D (VD) maximizes these health benefits, specifically for the circulatory and skeletal systems. This manuscript reviews VK's properties, molecular structures, nutrikinetics, mechanisms of action, daily requirements, safety in supplemental form, biomarkers used for its detection, and impacts on various organs. The purpose of synthesizing this information is to evaluate the potential uses of VK for the treatment or prevention of diseases.

Postprandial Plasma and Whole Blood Amino Acids Are Largely Indicative of Dietary Amino Acids in Adult Dogs Consuming Diets with Increasing Whole Pulse Ingredient Inclusion.

BACKGROUND: Pulse ingredients often replace grains in grain-free dog diets owing to their high-protein content. However, research to ascertain the benefit of this modification is limited. OBJECTIVES: This study aimed to correlate food compounds in 1 corn-inclusive control diet and 3 grain-free diets with increasing inclusions of whole pulses (≤45%; Pulse15, Pulse30, and Pulse45), formulated to meet similar macronutrient and micronutrient targets with postprandial amino acids (AAs) in healthy dogs >20 wk. METHODS: Diets were analyzed for biochemical compounds using tandem mass spectrometry. Twenty-eight outdoor-housed, healthy, adult Siberian Huskies were allocated to diet, and meal responses were analyzed at baseline and weeks 2, 4, 8, 16, and 20 with samples collected at fasted and 15, 30, 60, 90, 120, and 180 min after meal presentation. Blood AAs were analyzed by ultra performance liquid chromatography and differences across week, treatment, and time postmeal were analyzed in SAS Studio. Partial least squares regression was performed in SAS Studio using biochemical compounds in the diet as predictor variables and blood AAs as response variables. RESULTS: In plasma, Pulse45 had ∼32% greater postprandial Asn than Pulse15, and the control diet had ∼34% greater postprandial Leu and ∼35% greater Pro than Pulse15 (P < 0.05). In whole blood, Pulse30 had ∼23% greater postprandial Lys than the control diet, and the control diet had ∼21% greater postprandial Met and ∼18% greater Pro than Pulse45 and Pulse30, respectively (P < 0.05). Several phospholipids were correlated with postprandial AAs. Compounds in the urea cycle and glycine and serine metabolism were more enriched (P < 0.05) in plasma and whole blood, respectively. CONCLUSIONS: In macronutrient-balanced and micronutrient-balanced canine diets that differ in their inclusion of corn-derived compared with pulse-derived ingredients, postprandial changes in circulating AAs are largely indicative of the dietary AAs. This helps further our understanding of AA metabolism in healthy dogs fed grain-free diets.

Beyond the Bowl: Understanding Amino Acid Requirements and Digestibility to Improve Protein Quality Metrics for Dog and Cat Foods.

The determination of amino acid (AA) requirements for mammals has traditionally been done through nitrogen (N) balance studies, but this technique underestimates AA requirements in adult animals. There has been a shift toward researchers using the indicator amino acid oxidation (IAAO) technique for the determination of AA requirements in humans, and recently in dogs. However, the determination of AA requirements specific to adult dogs and cats at maintenance is lacking and the current requirements outlined by the National Research Council are based on a dearth of data and are likely underreporting the requirements of indispensable AA (IAA) for the population. To ensure the physiological requirements of our cats and dogs are met, we need methods to accurately and precisely measure digestibility. In vivo methods, such as ileal cannulation, are most commonly used, however, due to ethical considerations, we are moving away from animal models and toward in vitro methods. Harmonized static digestion models have the potential to replace in vivo methods but work needs to be done to have these methods more accurately represent the gastrointestinal tract (GIT) of cats and dogs. The Digestible IAA Score (DIAAS) is one metric that can help define protein quality for individual ingredients or mixed diets that uses AA SID estimates and ideally those can be replaced with in vitro AA digestibility estimates. Finally, we need accurate and reliable laboratory AA analyses to measure the AA present in complete diets, especially those used to quantify methionine (Met) and cysteine (Cys), both often limiting AAs in cat and dog diets. Together, this will guide accurate feed formulation for our companion animals to satisfy requirements while avoiding over-supplying protein, which inevitably contributes to excess N excretion, affecting both the environment and feed sustainability.

The Pulse of It: Dietary Inclusion of Up to 45% Whole Pulse Ingredients with Chicken Meal and Pea Starch in a Complete and Balanced Diet Does Not Affect Cardiac Function, Fasted Sulfur Amino Acid Status, or Other Gross Measures of Health in Adult Dogs.

BACKGROUND: Pulses are an attractive alternative protein source for all mammals; however, recent reports suggest that these ingredients may be related to developing dilated cardiomyopathy in dogs. OBJECTIVES: The primary objective of this study was to quantify the effects of dietary pulse intake by adult dogs on cardiac function using echocardiographic measurements and cardiac biomarkers N-terminal pro-B-type natriuretic peptide and cardiac troponin I (cTnI). Second, to investigate the effects of pulse consumption on plasma sulfur amino acid (SAA) concentrations as pulses are generally low in SAA and may limit taurine synthesis. Last, to assess the general safety and efficacy of feeding pulse-containing diets on canine body composition and hematological and biochemical indices. METHODS: Twenty-eight privately-owned domestic Siberian Huskies (13 females; 4 intact, and 15 males; 6 intact) with a mean age of 5.3 ± 2.8 y (± SD) were randomly assigned to 1 of 4 dietary treatments (n = 7/treatment), with equal micronutrient supplementation and increasing whole pulse ingredient inclusion (0%, 15%, 30%, and 45%) with pea starch used to balance protein and energy. RESULTS: After 20 wks of feeding, there were no differences (P > 0.05) in echocardiographic parameters, N-terminal pro-B-type natriuretic peptide, and cTnI concentrations among treatments or across time within treatment (P > 0.05), indicating no differences in cardiac function among treatments. Concentrations of cTnI remained below the safe upper limit of 0.2 ng/mL for all dogs. Plasma SAA status, body composition, and hematological and biochemical indices were similar among treatments and over time (P > 0.05). CONCLUSIONS: The results from this study suggest that increasing the inclusion of pulses up to 45% with the removal of grains and equal micronutrient supplementation does not impact cardiac function concurrent with dilated cardiomyopathy, body composition, or SAA status and is safe for healthy adult dogs to consume when fed for 20 wks.

Dietary intake of amino acids and vitamins compared to NRC requirements in obese cats undergoing energy restriction for weight loss.

BACKGROUND: This study aimed to determine if obese cats undergoing energy restriction for weight loss would meet the National Research Council's (NRC) indispensable amino acid and vitamin recommendations when fed a purpose-formulated diet. Thirty cats were placed into one of two groups; obese (BCS 8 to 9/9; n = 16) and lean (BCS 4 to 5/9; n = 14) and included in a non-randomized retrospective observational study. Cats were fed a veterinary weight loss food during a 4-week period of weight maintenance. Obese cats (O-MAINT) refers to obese cats during this period, L-MAINT to lean cats. After this initial 4-week period, the lean cats finished the study at this time and the 16 obese cats continued and were energy restricted for a 10-week period (O-RESTRICT). Analysis for dietary concentrations of indispensable amino acid and vitamin contents were performed. Daily food intakes were used to determine minimum, maximum and average daily intakes of individual nutrients for all three groups and compared against NRC 2006 minimum requirements (MR), adequate intakes (AI) and recommended allowances (RA) for adult cats. RESULTS: Over 10 weeks, O-RESTRICT cats lost 672 g ± 303 g, representing a weight loss rate of 0.94 ± 0.28% per week. Daily intake of the majority of indispensable amino acids and vitamins was greater than the NRC 2006 recommended allowance (RA per kg ideal body weight ^0.67), except for arginine, choline, crude protein, phenylalanine plus tyrosine and threonine. All O-RESTRICT cats had minimum, average, and maximum arginine intakes less than the NRC AI. Minimum daily intake of choline was below NRC RA for all O-RESTRICT cats and below NRC MR for two. All, except one, O-RESTRICT cats had a maximum and average choline intake below RA. CONCLUSIONS: All cats remained clinically healthy and showed no clinical signs of deficiency. Dietary choline and arginine requirements of obese cats as well as health risks associated with low dietary intake during energy restriction warrant further investigation.

Assessment of dog owners' knowledge relating to the diagnosis and treatment of canine food allergies.

Canine food allergies are the result of an immune-mediated hypersensitivity reaction to dietary proteins and can manifest as a variety of dermatologic and/or gastrointestinal clinical signs. Food elimination trials followed by provocation tests are used to diagnose food allergies; however, no research has been conducted to determine whether elimination trials and provocation tests are being properly implemented by pet owners. The objectives of this study were to determine the level of knowledge of dog owners regarding food allergies, and to investigate how dog owners approach diagnosis and treatment with their veterinarians. This information will provide veterinary teams with insight on how to work with dog owners to obtain successful diagnosis and treatment. The results indicate that appropriate diet selection for the food elimination trial, owner education on compliance during the trial, and re-challenging with the previous diet should be the focal points for veterinarians suspecting food allergies in a canine patient.

Évaluation des connaissances des propriétaires de chiens portant sur le diagnostic et le traitement des allergies alimentaires canines. Les allergies alimentaires canines sont le résultat d'une réaction d'hypersensibilité à médiation immunitaire face aux protéines alimentaires et elles peuvent se manifester par divers signes cliniques dermatologiques et/ou gastro-intestinaux. Les essais d'élimination d'aliments suivis de tests de provocation sont utilisés pour diagnostiquer les allergies alimentaires. Cependant, aucune recherche n'a été réalisée pour déterminer si les essais d'élimination et les tests de provocation sont mis en place de façon adéquate par les propriétaires. Les objectifs de cette étude étaient de déterminer le niveau de connaissances des propriétaires de chiens concernant les allergies alimentaires et d'étudier la façon dont les propriétaires de chiens envisagent le diagnostic et le traitement avec leur médecin vétérinaire. Ces renseignements permettront aux équipes vétérinaires de constater comment travailler avec les propriétaires de chiens afin d'obtenir un diagnostic et un traitement réussi. Les résultats indiquent que le bon choix d'alimentation pour les essais d'élimination des aliments, l'éducation des propriétaires pour la conformité durant les essais et de nouveaux tests avec l'alimentation antérieure devraient être les principaux sujets pour les médecins vétérinaires soupçonnant des allergies alimentaires chez un patient canin.(Traduit par Isabelle Vallières).

Concentrations of calcium, phosphorus, and vitamin D in human foods are not different among 4 food databases.

Concentrations of calcium, phosphorus, and vitamin D in similar foods were compared over 4 international food databases and results demonstrated no differences (P > 0.05) among the values from the databases. Therefore, the use of different food databases for homemade dog and cat diet formulation should not affect the final diet nutrient density prediction for these key nutrients.

Le calcium, le phosphore et la vitamine D dans les aliments pour humains dans quatre bases de données alimentaires ne sont pas différents. Les concentrations de calcium, de phosphore et de vitamine D ont été comparées dans quatre bases de données alimentaires internationales et les résultats n'ont pas montré de différence (P > 0,05) entre les aliments similaires. Par conséquent, l'utilisation de bases de données alimentaires différentes pour la conception d'un régime alimentaire maison pour chiens et chats, ne devrait pas affecter la prédiction de la densité nutritionnelle du régime alimentaire final pour ces nutriments cléfs.(Traduit par les auteurs).

Many Canadian dog and cat foods fail to comply with the guaranteed analyses reported on packages.

We compared analyzed nutrient contents of Canadian-specific dry dog and cat foods to the guaranteed analyses on packages and to the Association of American Feed Control Officials (AAFCO) 2018 nutrient targets to assess compliance with the Consumer Packaging and Labelling Act. We also explored differences in macronutrient content between species (dog and cat) and life stage for adult pet foods (all life stages and senior). Extruded dog (n = 16) or cat (n = 11) foods advertised as all life stage or senior, sold only in Canada, and carrying an AAFCO nutritional adequacy statement were selected. Proximate analyses and amino acid analyses were completed on all diets. Of the 27 foods, 25 met or exceeded the AAFCO nutrient recommendations. Only 9 foods met all nutrient content claims listed in their guaranteed analyses. Nutrient content between species or life stages was not different (P > 0.10).

Bon nombre d'aliments pour chiens et chats ne sont pas conformes aux garanties analyses rapportées sur bag. Nous avons comparé a analysé la teneur en éléments nutritifs du sec spécifiques au Canada chien et chat aliments pour la garantie d'analyses sur l'emballage et à l'Association of American Feed Control Officials (AAFCO) 2018 objectifs d'éléments nutritifs. Nous avons également examiné les différences de teneur en macronutriments entre espèces (chien et chat) ou de la vie des aliments pour animaux adultes (tous les stades de la vie et les cadres supérieurs). Chien extrudé (n = 16) ou de la nourriture pour chats (n = 11) annoncé comme tous les stades de la vie ou senior, vendu uniquement au Canada, et la réalisation d'un état nutritionnel approprié de l'AAFCO ont été sélectionnés. L'analyse immédiate et en acides aminés ont été effectuées sur tous les régimes alimentaires. De l'alimentation 27, 25 ont atteint ou dépassé les recommandations nutritionnelles de l'AAFCO. Cependant, seuls les régimes alimentaires 9 satisfait à toutes les allégations relatives à la teneur en éléments nutritifs énumérés dans leurs garanties d'analyses. Teneur en éléments nutritifs entre les espèces ou étapes de la vie n'était pas différent (P > 0,10).(Traduit par les auteurs).