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1.
Euro Surveill ; 29(32)2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39119722

RESUMO

Monkeypox virus (MPXV) is an emerging zoonotic pathogen with complex epidemiology necessitating rapid diagnosis and distinguishing between clades and subclades. The emerging Clade Ib lacks the genomic region used in the Clade I-specific assay from the Centers for Disease Control and Prevention. We report an MPXV real-time PCR to specifically detect Clade Ib. The assay demonstrated proficient sensitivity and specificity in 92 samples and can be included along other TaqMan-based assays to detect MPXV and distinguish between clades and subclades.


Assuntos
Monkeypox virus , Mpox , Reação em Cadeia da Polimerase em Tempo Real , Sensibilidade e Especificidade , Monkeypox virus/genética , Monkeypox virus/isolamento & purificação , Monkeypox virus/classificação , Reação em Cadeia da Polimerase em Tempo Real/métodos , Mpox/virologia , Mpox/diagnóstico , Humanos , Animais , Filogenia , DNA Viral/genética , DNA Viral/análise
2.
Euro Surveill ; 29(11)2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38487886

RESUMO

Since the beginning of 2023, the number of people with suspected monkeypox virus (MPXV) infection have sharply increased in the Democratic Republic of the Congo (DRC). We report near-to-complete MPXV genome sequences derived from six cases from the South Kivu province. Phylogenetic analyses reveal that the MPXV affecting the cases belongs to a novel Clade I sub-lineage. The outbreak strain genome lacks the target sequence of the probe and primers of a commonly used Clade I-specific real-time PCR.


Assuntos
Monkeypox virus , Mpox , Humanos , Monkeypox virus/genética , Mpox/diagnóstico , Mpox/epidemiologia , República Democrática do Congo/epidemiologia , Filogenia , Surtos de Doenças
3.
J Infect Dev Ctries ; 18(4): 600-608, 2024 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-38728644

RESUMO

INTRODUCTION: Human Mpox (formerly monkeypox) infection is an emerging zoonotic disease caused by the Mpox virus (MPXV). We describe the complete genome annotation, phylogeny, and mutational profile of a novel, sustained Clade I Mpox outbreak in the city of Kamituga in Eastern Democratic Republic of the Congo (DRC). METHODOLOGY: A cross-sectional, observational, cohort study was performed among patients of all ages admitted to the Kamituga Hospital with Mpox infection symptoms between late September 2023 and late January 2024. DNA was isolated from Mpox swabbed lesions and sequenced followed by phylogenetic analysis, genome annotation, and mutational profiling. RESULTS: We describe an ongoing Clade I Mpox outbreak in the city of Kamituga, South Kivu Province, Democratic Republic of Congo. Whole-genome sequencing of the viral RNA samples revealed, on average, 201.5 snps, 28 insertions, 81 deletions, 2 indels, 312.5 total variants, 158.3 amino acid changes, 81.66 intergenic variants, 72.16 synonymous mutations, 106 missense variants, 41.16 frameshift variants, and 3.33 inframe deletions across six samples. By assigning mutations at the proteome level for Kamituga MPXV sequences, we observed that seven proteins, namely, C9L (OPG047), I4L (OPG080), L6R (OPG105), A17L (OPG143), A25R (OPG151), A28L (OPG153), and B21R (OPG210) have emerged as hot spot mutations based on the consensuses inframe deletions, frameshift variants, synonymous variants, and amino acids substitutions. Based on the outcome of the annotation, we found a deletion of the D14L (OPG032) gene in all six samples. Following phylogenetic analysis and whole genome assembly, we determined that this cluster of Mpox infections is genetically distinct from previously reported Clade I outbreaks, and thus propose that the Kamituga Mpox outbreak represents a novel subgroup (subgroup VI) of Clade I MPXV. CONCLUSIONS: Here we report the complete viral genome for the ongoing Clade I Mpox Kamituga outbreak for the first time. This outbreak presents a distinct mutational profile from previously sequenced Clade I MPXV oubtreaks, suggesting that this cluster of infections is a novel subgroup (we term this subgroup VI). These findings underscore the need for ongoing vigilance and continued sequencing of novel Mpox threats in endemic regions.


Assuntos
Genoma Viral , Monkeypox virus , Mpox , Filogenia , Sequenciamento Completo do Genoma , Humanos , República Democrática do Congo/epidemiologia , Estudos Transversais , Monkeypox virus/genética , Monkeypox virus/classificação , Masculino , Mpox/virologia , Mpox/epidemiologia , Feminino , Adulto , Surtos de Doenças , Mutação , Adolescente , Adulto Jovem , Criança , Pré-Escolar , Pessoa de Meia-Idade , Estudos de Coortes
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