Dazed, confused, and then hungry: pesticides alter predator-prey interactions of estuarine organisms.

Like predators, contaminant stressors such as pesticides may have large and interacting effects on natural communities by removing species or altering behaviors and species interactions. Yet, few studies in estuarine systems have evaluated the effects of a single, low-dose exposure to pesticides on key predators. Here, we investigated the effects of a common pyrethroid (resmethrin) + synergist (piperonyl butoxide; PBO) mixture used for mosquito abatement on two life stages (adult and juvenile) of an important invertebrate estuarine predator, prey, and fishery species: the blue crab (Callinectes sapidus). The effects of resmethrin with PBO (Res-PBO) were assessed using behavioral and mesocosm experiments to link effects on individuals with changes in predator-prey interactions: (1) In static non-renewal exposures, crabs exposed to 1:3, 10:30, or 100:300 µg l-1 Res-PBO or PBO-alone had increased mortality and reduced locomotor ability within 1-12 h, with higher effects in adults than juveniles. (2) In mesocosms, sublethal exposure to 1:3 µg l-1 Res-PBO altered abult and juvnile foraging ability by  lowering the ability of adult crabs to cannibalize juvenile crabs but increasing juvenile crab foraging rates. Juvenile crabs were also more vulnerable to predation following pesticide exposure. Thus, a single, sublethal exposure to low, environmentally occurring pesticide concentrations reduced blue crab survivorship and locomotor functioning, and altered predator-prey interactions by changing foraging rates and increasing vulnerability to predators. Pesticide stressors may therefore play an important but underestimated role in shaping coastal ecosystems in which invertebrate predators are important and may contribute to U.S. blue crab population declines.

Interferon Gamma ELISPOT Testing as a Risk-Stratifying Biomarker for Kidney Transplant Injury: Results From the CTOT-01 Multicenter Study.

Previous studies suggest that quantifying donor-reactive memory T cells prior to kidney transplantation by interferon gamma enzyme-linked immunosorbent spot assay (IFNγELISPOT) can assist in assessing risk of posttransplant allograft injury. Herein, we report an analysis of IFNγELISPOT results from the multicenter, Clinical Trials in Organ Transplantation-01 observational study of primary kidney transplant recipients treated with heterogeneous immunosuppression. Within the subset of 176 subjects with available IFNγELISPOT results, pretransplant IFNγELISPOT positivity surprisingly did not correlate with either the incidence of acute rejection (AR) or estimated glomerular filtration rate (eGFR) at 6- or 12-month. These unanticipated results prompted us to examine potential effect modifiers, including the use of T cell-depleting, rabbit anti-thymocyte globulin (ATG). Within the no-ATG subset, IFNγELISPOT(neg) subjects had higher 6- and 12-month eGFRs than IFNγELISPOT(pos) subjects, independent of biopsy-proven AR, peak PRA, human leukocyte antigen mismatches, African-American race, donor source, and recipient age or gender. In contrast, IFNγELISPOT status did not correlate with posttransplant eGFR in subjects given ATG. Our data confirm an association between pretransplant IFNγELISPOT positivity and lower posttransplant eGFR, but only in patients who do not receive ATG induction. Controlled studies are needed to test the hypothesis that ATG induction is preferentially beneficial to transplant candidates with high frequencies of donor-reactive memory T cells.

Multicenter validation of urinary CXCL9 as a risk-stratifying biomarker for kidney transplant injury.

Noninvasive biomarkers are needed to assess immune risk and ultimately guide therapeutic decision-making following kidney transplantation. A requisite step toward these goals is validation of markers that diagnose and/or predict relevant transplant endpoints. The Clinical Trials in Organ Transplantation-01 protocol is a multicenter observational study of biomarkers in 280 adult and pediatric first kidney transplant recipients. We compared and validated urinary mRNAs and proteins as biomarkers to diagnose biopsy-proven acute rejection (AR) and stratify patients into groups based on risk for developing AR or progressive renal dysfunction. Among markers tested for diagnosing AR, urinary CXCL9 mRNA (odds ratio [OR] 2.77, positive predictive value [PPV] 61.5%, negative predictive value [NPV] 83%) and CXCL9 protein (OR 3.40, PPV 67.6%, NPV 92%) were the most robust. Low urinary CXCL9 protein in 6-month posttransplant urines obtained from stable allograft recipients classified individuals least likely to develop future AR or a decrement in estimated glomerular filtration rate between 6 and 24 months (92.5-99.3% NPV). Our results support using urinary CXCL9 for clinical decision-making following kidney transplantation. In the context of acute dysfunction, low values can rule out infectious/immunological causes of injury. Absent urinary CXCL9 at 6 months posttransplant defines a subgroup at low risk for incipient immune injury.

Hemoglobin Polymerization in Red Blood Cells of Marine Fishes: A Case of Adaptive Phenotypic Plasticity?

We investigated the occurrence of the unusual phenomenon of hemoglobin polymerization in a 10-year survey of 47 species of fishes. Similar to human sickle cell disease, hemoglobin polymers in fish red blood cells can cause distortion or sickling under low oxygen and low pH. We sampled fish from three geographic areas, including the east and west coasts of the Atlantic Ocean and the Gulf of Mexico. Fifteen species spanning five orders and nine families exhibited hemoglobin polymerization in vitro, with a majority in or related to Gadiformes, as well as species within Notocanthiformes, Perciformes, and Scorpianiformes. Atlantic cod, Gadus morhua, also showed the trait in vivo. Light and transmission electron microscopy confirmed the presence of hemoglobin polymers at the cellular level, but the morphology of hemoglobin polymers and rates of polymerization varied across species. Hemoglobin polymerization in red blood cells in vitro was pH dependent and reversible. For two species, G. morhua and Opsanus tau, >60% and >40% of all red blood cells contained hemoglobin polymers at pH 7.6, while 100% and 90% of red blood cells polymerized at pH 6.96, respectively. In both species, recovery of 60%-70% of red blood cells occurred within 45 minutes when pH increased from 6.96 to 7.99. From these results we conclude that hemoglobin polymerization is present in a broad range of fish taxa occupying wide biogeographical ranges and habitats and that it is oxygen and pH sensitive. The physiology and adaptive significance of hemoglobin polymerization in fishes remain unclear, but as oceans and coastal environments become more hypoxic and hypercapnic, this trait may have the potential to affect fish survival.

The interaction of epidermal growth factor and radiation in human head and neck squamous cell carcinoma cell lines with vastly different radiosensitivities.

PURPOSE: This study was performed to characterize the interaction of epidermal growth factor and radiation in two human head and neck squamous cell cancer cell lines of vastly different radiosensitivities (UM-SCC-6 Radiosensitive; UM-SCC-1 radioresistant). METHODS AND MATERIALS: The two human head and neck squamous cell cancers (UM-SCC-1 and UM-SCC-6) were grown in medium and following the appropriate treatments, cell survival was assessed by a standard colony formation assay. Growth inhibition was assessed by monitoring cell counts following treatment and flow cytometry was used to assess cell cycle distributions. RESULTS AND CONCLUSION: It was determined that exposure to epidermal growth factor (10 ng/ml) for 24 h prior to radiation resulted in radiosensitization in both cell lines, however, the magnitude of radiosensitization was greater in the radiosensitive UM-SCC-6 cells compared to the radioresistant UM-SCC-1 cells. Treatment of the UM-SCC-6 cells with epidermal growth factor (EGF) (10 ng/ml) for 24 h resulted in a growth delay, however, cell growth returned to normal approximately 24 h following removal of EGF. Similar treatment of the UM-SCC-1 cells resulted in no growth inhibition. The 24 h pre-radiation exposures to EGF (10 ng/ml) did not affect the radiation-induced growth delay in either cell line. Additionally, the 24 h exposures to EGF (10 ng/ml) did not affect the radiation-induced growth delay in either cell line. Additionally, the 24 h exposures to EGF (10 ng/ml) did not cause the cells to enter a more radiosensitive cell cycle phase. Further work will be necessary to determine whether events associated with the EGF-induced growth delay in the UM-SCC-6 cells are associated with the enhanced EGF-induced radiosensitization in these cells compared to UM-SCC-1 cells.

Cleft palate induction: quantitative studies of 3H corticoids in A/Jax mouse tissues after maternal injections of 3H cortisol.

A/Jax mice were injected with 50 muCi of 3H cortisol and 10 mg of cortisone acetate on day 12 of gestation. At various intervals after injection, the maternal liver, placenta, yolk sac, and the fetal jaws were removed and analyzed by thin-layer chromatography in conjunction with oxidative combustion and liquid scintillation counting to identify and measure the amount of labeled corticoid metabolites. The results support the thesis that these corticoids may induce clefts by an individual or combined direct action on the fetal jaws and palatal shelf tissue.

The status of the 1990 objectives for physical fitness and exercise.

During the past 5 years, considerable progress has been made in clarifying the relationship between physical activity and health and in collecting previously unavailable information about the activity levels of children and adults. Several of the objectives are likely to be achieved by 1990. Nevertheless, important questions remain to be answered, and data necessary to know the status of some objectives are not available. The established benefits of regular physical activity include a reduced risk of coronary heart disease, desirable weight control, and reduced symptoms of anxiety and mild-to-moderate depression. Other health effects appear likely, but are less firmly established. The salubrious effect of regular physical activity on reducing the risk of coronary heart disease appears to exist even at low levels of physical activity. More information is needed about the type and intensity of physical activity necessary to achieve the various health benefits ascribed to it. Although most people appear aware of the characteristics of activities likely to produce moderate to high levels of physical fitness, only 10-20 percent of the adult population participates in such activity. The number and effect of worksite physical fitness programs are too poorly documented to determine if the pertinent objectives can be achieved. Fewer than two-thirds of the number of children recommended in the objectives participate in daily physical education classes.

Congestive heart failure associated with endoscopic cholecystectomy: a case report.

Endoscopic cholecystectomies or laparoscopic cholecystectomies are becoming relatively commonplace in major medical centers throughout the United States. Very few anesthesia complications have been attributed to the procedure. This is a case report of congestive heart failure occurring during the procedure itself, which could have implications for patient management and selection.