Immunological status of patients with denture stomatitis and yeast infection after treatment of maxillofacial tumors.

Therapeutic modalities employed in the treatment of maxillofacial malignancy, tumor resection, radiation and chemotherapy, can result in local and systemic changes in healthy oral tissues many years after anticancer therapy has been stopped. The immunologic status of a select group of nine individuals who underwent surgery of the maxillofacial region and after head and neck irradiation, with severe and persistent denture stomatitis was studied. A significant impairment of granulocyte and lymphocyte functions and increased concentrations of serum IgG, as compared to healthy controls, were found in the patients of the study group. It is concluded, that this group of patients show immunological abnormalities many years post therapy, which must be recognized by the clinician in order to effect a cure.

Mutagenic activity of some textile dyes in different test systems.

The Salmonella/microsome mammalian test, the micronucleus and the dominant lethal tests on mice were used to study mutagenic effects of three dyes widely used in the textile industry. Direct Black 19:1, Direct Red 81 and Acid Blue 62 increased the frequency of micronucleated polychromatic erythrocytes in bone marrow of mice. Out of all dyes tested, only Direct Black 19:1 appeared to be an indirect mutagen inducing reverse mutations in two strains of Salmonella typhimurium TA 1538 and TA 98. None of them produced dominant lethal mutations in germ cells of male mice.

Absence of mutagenicity of trioxane and dioxolane in Salmonella typhimurium.

The mutagenic activity of trioxane and dioxolane was investigated in 5 histidine-requiring strains of S. typhimurium: TA1535, TA1537, TA1538, TA98 and TA100 with and without activation by liver microsomes. They were not mutagenic under any of these conditions with any of the his- S. typhimurium tester strains (in the presence or absence of S9 mix from liver homogenate of rats induced with Aroclor 1254).

Genotoxicity evaluation of tetramethylbenzenes.

The three tetramethyl isomers of benzene (prenitene, 1,2,3,4-; izodurene, 1,2,3,5-; and durene, 1,2,4,5-tetramethylben- zene) were studied using in vitro mutagenicity and in vivo genotoxicity tests. Potency of mutate induction by these solvents was evaluated in Salmonella typhimurium cells with, and without S9-mix made from Aroclor 1254-induced rat liver S9. The potency of induction of micronuclei (MN) and sister chromatid exchanges (SCEs) by solvents was evaluated in bone marrow of mice. Izodurene displayed mutagenic potency in strains TA97a, TA98 and TA100 only in the absence of the S9-mix. In MN tests, all three tetramethylbenzenes demonstrated no clastogenic activity on the bone marrow cells. All the tested solvents were active as genotoxic compounds in the SCE tests, demonstrating a dose-response relationships.

Genotoxicity of Farbasol and its component: 4-ethyltoluene.

A combination of assays for gene mutations in Salmonella typhimurium TA97a, TA98, TA100 and TA102 strains with and without rat liver activation, and for micronucleus and sister chromatid exchange (SCE) in bone marrow cells of Imp:Balb/c mice was used to provide data on the mutagenic and genotoxic properties of the mixture of aromatic solvents, known under the trade name of Farbasol. In addition, 4-ethyltoluene (the main ethylmethylbenzenic component of Farbasol) was also tested for muta- and genotoxicity. The results revealed that neither Farbasol nor 4-ethyltoluene induced an increased reverse mutation in bacterial cells or the formation of micronucleated polychromatic erythrocytes in bone marrow. However, those compounds were found to be active as sister chromatid exchange (SCE) agents.

Genotoxicity evaluation of trimethylbenzenes.

The three trimethyl isomers of benzene (hemimellitene, 1,2,3-TMB; pseudocumene, 1,2,4-TMB and mesitylene, 1,3,5-TMB) were investigated for different genotoxicity endpoints: in vitro, in the Ames test with Salmonella typhimurium TA97a, TA98, TA100 and TA102 strains in the presence and absence of rat liver S9 metabolic activation; in vivo, in the micronucleus and sister chromatid exchange (SCE) tests with bone marrow cells of Imp:Balb/c mice. Only the isomer of benzene with the methyl-group at position 1, 2, 3 was found to have mutagenic effect on S. typhimurium cells. Increase in bacterial reversions was observed in four conventional strains used in this study, but most clearly in TA97a. The mutagenic responses of 1,2,3-TMB with the SalmonellaL tester strains were observed in the experiments performed in the absence of enzymatic activation. None of the compounds had an influence on the frequency of micronucleated polychromatic erythrocytes in bone marrow cells of mice. However, all the three compounds were observed to have a cytogenetic potential of increasing the SCE level in these cells. Significant responses in SCE induction, compared with the level of those changes in corresponding solvent-administered controls, were obtained at three test doses of 1,2,3-TMB (730, 1470, 2200 mg/kg) and 1,2,4-TMB (900, 1800, 2700 mg/kg) and at two doses of 1,3,5-TMB (1800, 2700 mg/kg). These data provided a limited evidence for the genotoxic activity of 1,2,3-TMB and inadequate evidence for genotoxic activity of 1,2,4-TMB and 1,3,5-TMB.

Genotoxicity assessment of new synthesized acridine derivative--3,6-diamino-10-methyl-9,10-dihydroacridine.

A new synthesized acridine derivative, 3,6-diamino-10-methyl-9, 10-dihydroacridine (AcrH), was tested for in vitro reverse mutations with Salmonella TA strains, chromosome aberrations and sister chromatid exchanges (SCE) in human lymphocytes, and for in vivo chromosome aberrations in bone marrow of mice. Using the classic plate incorporation method, mutagenicity of AcrH in bacterial cells (TA97a, TA98, TA100 and TA102) was observed in the experiments performed with, and without, rat liver S9 metabolic activation. The reverse mutation assay showed no difference in mutagenic activity between AcrH and acriflavine (Acr(+)) in the test with TA97. The results of in vitro chromosome aberrations assay revealed potential clastogenicity. The test using macroculture of human lymphocytes induced mainly chromatid gaps. The experiments with human lymphocytes revealed SCE-inducing effect of AcrH and Acr(+). In an in vivo study, AcrH given intraperitoneally to Balb/c mice did not cause any significant increase in the percentage of cells with aberrations compared to the negative control.

Non-mutagenicity of thiadiazole-1,3,4 derivatives in Salmonella typhimurium strains.

The Ames mutagenicity assay with Salmonella typhimurium strains (TA97a, TA98, TA100 and TA102) was used to investigate the ability of five dyes (thiadiazole-1,3,4 derivatives) to induce point mutations. The results did not reveal any mutagenic effects of the tested dyes on the S. typhimurium strains. No mutagenic activity was observed in the experimental series with and without exogenous activation.

Comparison of the mutagenicity of chemical agents released during coke production.

Benzene- and acetone-extracted samples of airborne particulate matter collected in two coke plants were analysed for their content of genotoxic agents (coal tar pitch volatiles (CTPVs), polycyclic aromatic hydrocarbons (PAHs): benzo/a/pyrene, pyrene, dibenzo/a,h/anthracene, anthracene and fluoranthene) following the identification and quantification of the compounds as well as the evaluation of their mutagenic activity using in vitro reverse mutation assay. Results of determinations at the top section of coke batteries were compared. Mean concentrations of total dust, CTPVs and B/a/P were as follows: 2.06 mg/m3, 0.63 micrograms/m3 in plant A, and 2.77 mg/m3, 0.89 mg/m3 and 0.35 micrograms/m3 in plant B, respectively. All the extracts of particulate matter samples increased the induction of bacterial mutation in both +S9 and -S9 tests. The exposures to mutagenic airborne particulate matter were in the range of 98-683 rev./m3 (-S9): 714-2214 rev./m3 (+S9) in plant A, and 21-496 rev./m3 (-S9); 68-850 rev./m3 (+S9) in plant B. The correlation between mutagenic potential and concentrations of tested agents was insignificant. The coke oven emission seemed to be higher in plant A with side charging of coal.

Cyanuric chloride has no genotoxic and mutagenic properties in bacteria and bone marrow cells.

Three short-term tests were used; Salmonella/microsome assay, in vivo sister chromatid exchange assay (SCE) and micronucleus assay to evaluate mutagenic and genotoxic properties of 2,4,6-trichlorotriazine; cyanuric chloride. Mutagenicity assays were carried out using the standard top agar overplay plate assay described by Maron and Ames (9). Tester strains TA97a, TA98, TA100 and TA102 were used. Compound was dissolved in 0.1 ml of DMSO and doses of 1, 10, 100 and 500 micrograms/plate were tested in the absence and in the presence of the S9-mix. From the results obtained it appeared that incubation of the test substance with the bacteria did not increase the number of His+ revertants with any of the strains of S. typhimurium, either in the absence or in the presence of the S9-mix. At the high dose level used i.e. 100 and 500 micrograms/plate, the test substance appeared to be slightly toxic for strain TA 97a (in the absence of the S9-mix), as was seen from a diminished number of revertant colonies. The SCE test was performed according to the GENE-TOX programme. No significant increase was noted in the incidence of SCE in the groups treated with all tested doses of cyanuric chloride. Thus, in this test cyanuric chloride did not induce chromosomal damage resulting in SCE formation in bone marrow cells of mice. The micronucleus assay in vivo was performed on mice bone marrow cells. The incidence of micronucleated polychromatic erythrocytes after administration of all doses of cyanuric chloride used were not statistically different (p > 0.05) as compared to negative controls.(ABSTRACT TRUNCATED AT 250 WORDS)

Mutagenic and genotoxic activity detected by the Ames, micronucleus and SCE tests under the influence of samples of dyes manufactured in Poland.

In this study, we evaluated the mutagenic and genotoxic potential of commercial samples of chemicals manufactured by Polish dyestuff industry for their ability to mutate Salmonella typhimurium strains (TA97a, TA98, TA100 and TA102), and to induce formation of micronuclei (MN) and sister chromatid exchanges (SCE) in mice bone marrow cells. This study involved five dyes selected from a list of colorants, considered to be representative of those which were most extensively used in the trade. According to criteria listed by IARC (1984), the results obtained in this work and earlier tests of Basic Blue 26, 44045 and Direct Red 83, 29225 provided no evidence of genetic activity (all 3 tests were negative); for Acid Violet 49, 42640 and Disperse Blue 37, the evidence of genetic activity was inadequate (1 test was positive) and the tests for Acid Black 194 provided limited evidence of genetic activity (2 positive tests).

Mutagenic activity of 3,6-diamino-10-methyl-9,10-dihydroacridine in Salmonella typhimurium cells.

Mutagenic evaluation of 3,6-diamino-10-methyl-9,10-dihydroacridine (AcrH), a new synthesized acridine derivative was undertaken using short-term in vitro test. The mutagenic potential of AcrH was evident from the reverse mutation induced in the presence and absence of S9-mix of Salmonella typhimurium TA97a, TA98, TA100 and TA102 cells. The levels of the mutagenic activity of AcrH in TA97a are comparable with that of acriflavine.

Genotoxicity assessment of Rokanol B2 and Rokamid R1.

Two surface-active compounds, Rokanol B2 and Rokamid R1, were examined with three types of screening tests: 1. standard Ames test in vitro using S. typhimurium TA97a, TA98, TA100 and TA102 strains; 2. micronucleus test in vivo; 3. sister chromatid exchange (SCE) on bone marrow cells of Balb C mice. Negative results of the testing, in terms of the effects of activity of both the technical preparations on S. typhimurium cells and bone marrow of Balb C mice, were found for both tested chemicals.

In vitro study on the inhibiting effect of different agents on the growth of Candida albicans on acrylic resin surfaces.

This study evaluated at the in vitro level the antifungal effectiveness of nystatin, chlorhexidine, and a homologous histidine polypeptide on the surface of acrylic resin disks. The agents were used in a way that simulated storage of a denture by a denture wearer. Results indicated that pretreatment with poly-L-histidine was not protective against C albicans adherence and growth regardless of whether disks were stored in water or in the open air for the 8-hour period following yeast contamination. Chlorhexidine was totally effective in preventing C albicans attachment to, and growth on, the acrylic resin, even after a period of 8 days of turbidimetric monitoring. Pretreatment with Nystatin, followed by drying, was protective, yielding results similar to those obtained with chlorhexidine.

Sensitivity of the replica method in the detection of candidal infection among denture wearers with clinically healthy oral mucosa.

To ascertain the role of Candida in denture stomatitis, the practitioner must conduct a mycologic examination of the acrylic resin denture surface, because it acts as a reservoir for continuous reinfection of the palate. Twenty-two patients were examined to compare the sensitivity of the standard technique of swabbing the denture to that of a newly developed cast agar replica technique for detecting Candida albicans. The dentures were swabbed and cast replicas of the tissue-fitting surface of the dentures were made of both study populations. The majority of cultures obtained by swabbing failed to detect the presence of Candida albicans, while all cast agar replicas grew Candida albicans. The replica method for the detection of Candida albicans in edentulous patients seemed to be a more sensitive method than currently available mycologic methods.

Experimental investigations on sensitivity to nickel present in alloys used in dentistry carried out on guinea pigs previously sensitized to this metal.

Experimental investigation on sensitivity to nickel present in dental alloys has been carried out on guinea pigs previously sensitized to this metal. The metal plates containing nickel have been sutured to the oral mucosa in such a way that condition of alloys contact with mucosa were possible similar to those present in the mouth of the patient wearing fixed prosthesis. The results of macro and micro examination has not shown any morphological differences between experimental and control material.

Potential carcinogenicity assessment of alpha-aescin and phenbendasol.

Potential carcinogenic activity of alpha-aescin and phenbendasole made by "Polfa" (Poland) as well as phenbendasole produced by "Hoechst" (Germany) was studied using Salmonella/microsome test, DNA repair test and micronucleus assay. None of tested preparations were mutagenic or genotoxic what suggest that none of them possess potential carcinogenic activity. Besides, it was established that alpha-aescin exhibits strong and phenbendasol weak acute systemic toxicity for mice. Alpha-aescin and phenbendasole produced in Poland have been found to be toxic for bone marrow cells of mice but only when administered at a high dose of 80% LD50.

A long-term follow-up of allergy to nickel among fixed prostheses wearers.

Skin allergic hypersensitivity to nickel is well known and well documented in the professional literature. Sixteen partially dentate patients with long-standing histories of recurrent hypersensitive skin reaction to nickel were entered into a clinical trial in 1979. All the patients received crowns or bridges made of an alloy containing 66% of nickel with porcelain fused to the metal framework. During the first three years, annual clinical dental and dermatological investigations were performed on all the subjects, including repeated patch tests. Follow-up examinations took place in 1988 and 1994. None of the subjects had any mucosal or systemic reaction following oral exposure to the nickel containing alloy, nor the exacerbations of skin lesions.

[Methods used in evaluating mutagenic and genotoxic properties of chemical compounds. II. The Ames test (an abridged version)]. / Metodyki stosowane do oceny wlasciwosci mutagennych i genotoksycznych zwiazków chemicznych. Czesc II. Plytkowy test Amesa (wersja skrócona).

The paper presents in vitro the technique developed by Ames et al (1.5), applied in screening tests for evaluations of mutagenic effects of chemical substances. As the experimental model in the test use histidine-dependent bacterial cells of Salmonella typhimurium series TA strains. Chemical substances biotransformation is carried out in the presence of microsomal activating system (fraction S9), mostly obtained from rat's liver. Mutagenic activity of chemical substances is measured by an induction of his- mutation reversion in S. typhimurium strains, expressed as number His+ of revertant colonies, as compared to number His+ of spontaneous revertant colonies of a given strain on Petri dishes containing minimal glucose agar after 48-72 hr incubation at 37 degrees C.

[Identification of potential carcinogenic dyes and intermediates on the basis of their genotoxicity]. / Identyfikacja potencjalnie rakotwórczych barwników i pólproduktów na podstawie oceny ich genotoksycznosci.

41 dyes, pigments and surface-active compounds were tested for mutagenic and genotoxic properties. It was found that 43% of the studied dyes of Polish make which belonged to the azo, triarylmethane and anthraquinone compounds were mutagenic or genotoxic. All the studied pigments and surface active compounds did not reveal potential carcinogenic activity except for NNO Dyspergator whose possible genotoxic activity needs confirmation in further study.