Performance Evaluation of Focal Plane Array (FPA)-FTIR and Synchrotron Radiation (SR)-FTIR Microspectroscopy to Classify Rice Components.

The development of biochemical analysis techniques to study heterogeneous biological samples is increasing. These techniques include synchrotron radiation Fourier transform infrared (SR-FTIR) microspectroscopy. This method has been applied to analyze biological tissue with multivariate statistical analysis to classify the components revealed by the spectral data. This study aims to compare the efficiencies of SR-FTIR microspectroscopy and focal plane array (FPA)-FTIR microspectroscopy when classifying rice tissue components. Spectral data were acquired for mapping the same sample areas from both techniques. Principal component analysis and cluster imaging were used to investigate the biochemical variations of the tissue types. The classification was based on the functional groups of pectin, protein, and polysaccharide. Four layers from SR-FTIR microspectroscopy including pericarp, aleurone layer, sub-aleurone layer, and endosperm were classified using cluster imaging, while FPA-FTIR microspectroscopy could classify only three layers of pericarp, aleurone layer, and endosperm. Moreover, SR-FTIR microspectroscopy increased the image contrast of the biochemical distribution in rice tissue more efficiently than FPA-FTIR microspectroscopy. We have demonstrated the capability of the high-resolution synchrotron technique and its ability to clarify small structures in rice tissue. The use of this technique might increase in future studies of tissue characterization.

Using synchrotron based ATR-FTIR, EXAFS, and XRF to characterize the chemical compositions of TSP in industrial estate area.

In Thailand, the Map Ta Phut Industrial Estate (MTPIE), a prominent industrial hub, has substantial environmental and health issues caused by industrial pollution. This study uses advanced synchrotron-based techniques, such as Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR), Extended X-ray Absorption Fine Structure (EXAFS), and X-ray Fluorescence (XRF), to fully examine the chemical make-up of total suspended particulate (TSP) in the given area. Notable findings include the detection of remarkably high enrichment factors for magnesium and sulfur, indicating the presence of industrial operations. Additionally, we found that magnetite, which accounts for an average of 40 % of the total iron oxides in the samples, is the main iron oxide. The study also highlights about how calcium carbonate and different organic functional groups are found in large amounts, which shows that industrial emissions and natural sources are connected in a complex way. The findings underscore the susceptibility of children to TSP exposure, revealing increased rates of inhalation and significant health hazards. In order to safeguard public health in industrial areas such as MTPIE, it is imperative to implement more sophisticated pollution control techniques and maintain ongoing environmental monitoring.