RESUMO
Helicobacterpylori, a common infectious bacterium, has been linked to chronic gastritis, peptic ulcer and gastric cancer. Gastric biopsy specimens were obtained from 58 northern Thai patients with gastritis, 28 with gastric ulcer, 45 with duodenal ulcer and 4 with gastric cancer. cagA, vacA s1 and iceA gene was found in 88, 98, and 89% of the specimens, respectively. For vacA, the frequency of subtype s1a, s1c and combined sla and s1c was 40, 16, and 41%, respectively. The frequency of subtype s1a/m1 and s1a/s1c/m1 was 27 and 20%, respectively. Fifty-three patients (39%) were infected with multiple vacA genotypes but there was no association with clinical outcome. cagA positive and mixed vacA s1a and s1c strains were found in significantly more cases of duodenal ulcer than gastritis (p < 0.05). For iceA, subtype iceA1 reached a frequency of 60%, whereas subtype iceA2 was only 24%.
Assuntos
Antígenos de Bactérias/genética , Proteínas da Membrana Bacteriana Externa/genética , Proteínas de Bactérias/genética , Gastroenteropatias/microbiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/genética , Gastroenteropatias/genética , Genótipo , Infecções por Helicobacter/genética , Helicobacter pylori/isolamento & purificação , Helicobacter pylori/patogenicidade , Humanos , Reação em Cadeia da Polimerase , Fatores de Risco , Tailândia , VirulênciaRESUMO
The STR loci D10S2325, D16S539 and D19S253 were analyzed in 203 unrelated Northern Thai. A power of discrimination of 0.954, 0.923 and 0.921 and a power of exclusion of 0.690, 0.542 and 0.632, respectively were found. The combined power of discrimination and exclusion reached 0.99972 and 0.9478, respectively. No deviation from the Hardy-Weinberg equilibrium was observed for the three loci.
Assuntos
Frequência do Gene , Genética Populacional , Sequências de Repetição em Tandem , Impressões Digitais de DNA , Humanos , Reação em Cadeia da Polimerase , TailândiaRESUMO
Nine STR loci (D3S1358, D5S818, D7S820, D8S1179, D13S317, TH01, VWA, TPOX, LPL) were studied in a large Northern Thai population sample. All loci meet Hardy-Weinberg expectations. The combined power of discrimination and exclusion is 0.9999999979 and 0.99798 respectively. Mutation rates for STR loci did not exceed 1-4 per 1000 parent/child pairs as derived from disputed paternity cases. Similar mutation rates were also reported from other populations. No mutations were found for D5S818, D7S820, TH01, and TPOX.
Assuntos
Frequência do Gene , Genética Populacional , Sequências de Repetição em Tandem , Impressões Digitais de DNA , Humanos , Reação em Cadeia da Polimerase , TailândiaRESUMO
We designed new primers that give shorter amplicons for Penta D and Penta E for easy separation on native polyacrylamide gels. 112 unrelated Northern Thai men were typed. A total of nine and eight-teen alleles was found for Penta D and Penta E, respectively. Both loci showed a high power of discrimination (Penta D: 0.927; Penta E: 0.966; both loci combined: 0.9975) as reported in other populations. Penta D met Hardy-Weinberg expectations, whereas Penta E did not, maybe because of the small sample size in relation to the large number of alleles of this locus.
Assuntos
Povo Asiático/genética , Sequências de Repetição em Tandem , Alelos , Sequência de Bases , Cromossomos Humanos Par 15/genética , Cromossomos Humanos Par 21/genética , Primers do DNA , Eletroforese em Gel de Poliacrilamida , Medicina Legal , Genética Populacional , Humanos , Masculino , Dados de Sequência Molecular , TailândiaRESUMO
Primers currently used for amplification of locus DYS385 on the Y chromosome give quite long amplicons that are difficult to resolve on native polyacrylamide gels. We therefore designed new primers that give much shorter products for easy separation. With this system, two allele variants that were characterized by sequencing as 14.1 (GAAA --> GAAAA) and 14.2 (GA insertion), were identified in two unrelated Northern Thai men due to their decreased mobility. Interestingly, initial capillary electrophoresis mistyped the 14.1 variant amplified with the traditional primers as allele 14. Forty different haplotypes were found in 147 Northern Thais with a haplotype diversity of 0.9430. Haplotype 13-18 (frequency 0.136) is the most frequent one: for comparison: haplotype 14-18 (frequency 0.074) was found to be the most common in a population from Bangkok (n = 95) in an earlier study. This striking difference within Thailand's population probably results from genetic differences in the founding populations.
Assuntos
Alelos , Primers do DNA , Variação Genética , Genética Populacional , Haplótipos , Cromossomos Humanos Y , Impressões Digitais de DNA/métodos , Humanos , Masculino , Reação em Cadeia da Polimerase , Sequências de Repetição em Tandem , TailândiaRESUMO
Helicobacterpylori, a common infectious bacterium, has been linked to chronic gastritis, peptic ulcer and gastric cancer. Gastric biopsy specimens were obtained from 58 northern Thai patients with gastritis, 28 with gastric ulcer, 45 with duodenal ulcer and 4 with gastric cancer. cagA, vacA s1 and iceA gene was found in 88, 98, and 89% of the specimens, respectively. For vacA, the frequency of subtype s1a, s1c and combined sla and s1c was 40, 16, and 41%, respectively. The frequency of subtype s1a/m1 and s1a/s1c/m1 was 27 and 20%, respectively. Fifty-three patients (39%) were infected with multiple vacA genotypes but there was no association with clinical outcome. cagA positive and mixed vacA s1a and s1c strains were found in significantly more cases of duodenal ulcer than gastritis (p < 0.05). For iceA, subtype iceA1 reached a frequency of 60%, whereas subtype iceA2 was only 24%.