RESUMO
Although some bacteria require an atmosphere with high CO(2) levels for their growth, CO(2) is not generally supplied to conventional screening cultures. Here, we isolated 84 bacterial strains exhibiting high-CO(2) dependence. Their phylogenetic affiliations imply that high-CO(2) culture has potential as an effective method to isolate unknown microorganisms.
Assuntos
Actinobacteria/crescimento & desenvolvimento , Actinobacteria/isolamento & purificação , Dióxido de Carbono/metabolismo , Proteobactérias/crescimento & desenvolvimento , Proteobactérias/isolamento & purificação , Actinobacteria/classificação , Actinobacteria/metabolismo , Biodiversidade , Contagem de Colônia Microbiana , DNA Bacteriano/isolamento & purificação , Concentração de Íons de Hidrogênio , Japão , Filogenia , Proteobactérias/classificação , Proteobactérias/metabolismo , RNA Ribossômico 16S/análise , Análise de Sequência de DNA , Microbiologia do Solo , Microbiologia da ÁguaRESUMO
A novel xylan-degrading bacterium, YT-1101(T), was isolated from fresh water. The isolate was a Gram-reaction-negative, aerobic, motile, endospore-forming and rod-shaped bacterium. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain YT-1101(T) belonged to the genus Cohnella, sharing sequence similarities of less than 94 % with the type species. The genomic G+C content was 58.6 mol%. The predominant menaquinone was MK-7. The major fatty acids were anteiso-C(15 : 0), iso-C(16 : 0) and iso-C(15 : 0). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. On the basis of morphological, physiological and phylogenetic properties, strain YT-1101(T) represents a novel species of the genus Cohnella, for which the name Cohnella fontinalis sp. nov. is proposed. The type strain is YT-1101(T) (=NBRC 104957(T) =DSM 21753(T)).
Assuntos
Paenibacillus/genética , Aerobiose , Dados de Sequência Molecular , Paenibacillus/classificação , Paenibacillus/crescimento & desenvolvimento , Paenibacillus/isolamento & purificação , Paenibacillus/fisiologia , Filogenia , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Microbiologia da Água , Xilanos/metabolismoRESUMO
A Gram-negative, strictly aerobic, motile, filamentous and viscous exopolymer-producing bacterium, designated strain YT21(T), was isolated from fresh water. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain YT21(T) lies within a cluster containing the established genera Segetibacter, Terrimonas, Niastella and Chitinophaga in the phylum Bacteroidetes. However, the isolate represented a lineage distinct from these genera, with sequence similarities ranging from 88.9 to 91.8 %. The genomic G+C content was 45.2 mol%. The predominant isoprenoid quinone was MK-7. The major fatty acids were 15 : 0 iso, 17 : 0 iso 3-OH and 15 : 1 iso G. On the basis of morphological features and phenotypic and phylogenetic data, strain YT21(T) represents a novel genus and species, for which the name Filimonas lacunae gen. nov., sp. nov. is proposed. The type strain of Filimonas lacunae is strain YT21(T) (=NBRC 104114(T) =DSM 21054(T)).