Acoustic detection range of right whale upcalls identified in near-real time from a moored buoy and a Slocum glider.

The goal of this study was to characterize the detection range of a near real-time baleen whale detection system, the digital acoustic monitoring instrument/low-frequency detection and classification system (DMON/LFDCS), equipped on a Slocum glider and a moored buoy. As a reference, a hydrophone array was deployed alongside the glider and buoy at a shallow-water site southwest of Martha's Vineyard (Massachusetts, USA) over a four-week period in spring 2017. A call-by-call comparison between North Atlantic right whale upcalls localized with the array (n = 541) and those detected by the glider or buoy was used to estimate the detection function for each DMON/LFDCS platform. The probability of detection was influenced by range, ambient noise level, platform depth, detection process, review protocol, and calling rate. The conservative analysis of near real-time pitch tracks suggested that, under typical conditions, a 0.33 probability of detection of a single call occurred at 6.2 km for the buoy and 8.6-13.4 km for the glider (depending on glider depth), while a 0.10 probability of detection of a single call occurred at 14.4 m for the buoy and 22.6-27.5 km for the glider. Probability of detection is predicted to increase substantially at all ranges if more than one call is available for detection.

Variation in egg mass within two Atlantic herring Clupea harengus stocks.

The main objective of this study was to investigate if egg size (mass) at spawning is invariant for Scotia-Fundy summer and autumn (SFSH) and Icelandic summer (ISSH) spawning herring Clupea harengus. Oocyte dry mass measurements for SFSH females collected in 2001 and ISSH females collected in 1999 and 2000 showed a large variation. Difference in egg dry mass among fish was found to vary by as much as twofold in each stock. For ISSH, variation in egg mass was also apparent from oocyte volume measurements made jointly with a histological examination of the ovaries. Approximately 20% of the variation in egg mass could be explained by maternal whole-body mass or total length, indicating that length or age composition in the stocks can potentially influence the recruitment success. This implies that fisheries management strategies should aim to maintain a broad range in age composition.

Measuring abnormal movements in free-swimming fish with accelerometers: implications for quantifying tag and parasite load.

Animal-borne data loggers allow movement, associated behaviours and energy expenditure in fish to be quantified without direct observations. As with any tagging, tags that are attached externally may adversely affect fish behaviour, swimming efficiency and survival. We report on free-swimming wild Atlantic cod (Gadus morhua) held in a large mesocosm that exhibited distinctly aberrant rotational swimming (scouring) when externally tagged with accelerometer data loggers. To quantify the phenomenon, the cod were tagged with two sizes of loggers (18 and 6 g; <2% body mass) that measured tri-axial acceleration at 50 Hz. An automated algorithm, based on body angular rotation, was designed to extract the scouring movements from the acceleration signal (98% accuracy). The algorithm also identified the frequency pattern and associated energy expenditure of scouring in relation to tag load (% body weight). The average per cent time spent scouring (5%) was independent of tag load. The vector of the dynamic body acceleration (VeDBA), used as a proxy for energy expenditure, increased with tag load (r(2)=0.51), and suggests that fish with large tags spent more energy when scouring than fish with small tags. The information allowed us to determine potential detrimental effects of an external tag on fish behaviour and how these effects may be mitigated by tag size. The algorithm can potentially identify similar rotational movements associated with spawning, courtship, feeding and parasite-load shedding in the wild. The results infer a more careful interpretation of data derived from external tags and the careful consideration of tag type, drag, buoyancy and placement, as well as animal buoyancy and species.

Accelerometer tags: detecting and identifying activities in fish and the effect of sampling frequency.

Monitoring and measuring the behaviour and movement of aquatic animals in the wild is typically challenging, though micro-accelerometer (archival or telemetry) tags now provide the means to remotely identify and quantify behavioural states and rates such as resting, swimming and migrating, and to estimate activity and energy budgets. Most studies use low-frequency (≤32 Hz) accelerometer sampling because of battery and data-archiving constraints. In this study we assessed the effect of sampling frequency (aliasing) on activity detection probability using the great sculpin (Myoxocephalus polyacanthoceaphalus) as a model species. Feeding strikes and escape responses (fast-start activities) and spontaneous movements among seven different great sculpin were triggered, observed and recorded using video records and a tri-axial accelerometer sampling at 100 Hz. We demonstrate that multiple parameters in the time and probability domains can statistically differentiate between activities with high detection (90%) and identification (80%) probabilities. Detection probability for feeding and escape activities decreased by 50% when sampling at <10 Hz. Our analyses illustrate additional problems associated with aliasing and how activity and energy-budget estimates can be compromised and misinterpreted. We recommend that high-frequency (>30 Hz) accelerometer sampling be used in similar laboratory and field studies. If battery and/or data storage is limited, we also recommend archiving the events via an on-board algorithm that determines the highest likelihood and subsequent archiving of the various event classes of interest.

Absolute probability estimates of lethal vessel strikes to North Atlantic right whales in Roseway Basin, Scotian Shelf.

Vessel strikes are the primary source of known mortality for the endangered North Atlantic right whale (Eubalaena glacialis). Multi-institutional efforts to reduce mortality associated with vessel strikes include vessel-routing amendments such as the International Maritime Organization voluntary "area to be avoided" (ATBA) in the Roseway Basin right whale feeding habitat on the southwestern Scotian Shelf. Though relative probabilities of lethal vessel strikes have been estimated and published, absolute probabilities remain unknown. We used a modeling approach to determine the regional effect of the ATBA, by estimating reductions in the expected number of lethal vessel strikes. This analysis differs from others in that it explicitly includes a spatiotemporal analysis of real-time transits of vessels through a population of simulated, swimming right whales. Combining automatic identification system (AIS) vessel navigation data and an observationally based whale movement model allowed us to determine the spatial and temporal intersection of vessels and whales, from which various probability estimates of lethal vessel strikes are derived. We estimate one lethal vessel strike every 0.775-2.07 years prior to ATBA implementation, consistent with and more constrained than previous estimates of every 2-16 years. Following implementation, a lethal vessel strike is expected every 41 years. When whale abundance is held constant across years, we estimate that voluntary vessel compliance with the ATBA results in an 82% reduction in the per capita rate of lethal strikes; very similar to a previously published estimate of 82% reduction in the relative risk of a lethal vessel strike. The models we developed can inform decision-making and policy design, based on their ability to provide absolute, population-corrected, time-varying estimates of lethal vessel strikes, and they are easily transported to other regions and situations.

Efficacy of a voluntary area to be avoided to reduce risk of lethal vessel strikes to endangered whales.

Ocean-going vessels pose a threat to large whales worldwide and are responsible for the majority of reported deaths diagnosed among endangered North Atlantic right whales (Eubalaena glacialis). Various conservation policies have been implemented to reduce vessel-strike mortality in this species. The International Maritime Organization adopted the Roseway Basin Area to be avoided on the Scotian Shelf as a voluntary conservation initiative to reduce the risk of lethal vessel strikes to right whales. We initiated the Vessel Avoidance & Conservation Area Transit Experiment to evaluate the efficacy of this initiative because the effectiveness of the avoidance scheme in reducing risk without the imposition of vessel-speed restrictions depends entirely on vessel-operator compliance. Using a network of automatic identification system receivers, we collected static, dynamic, and voyage-related vessel data in near real time from the Roseway Basin region for 12 months before and 6 months after the implementation of the area to be avoided. Using pre- and post-implementation vessel navigation and speed data, along with right whale sightings per unit effort data, all resolved at 3'N latitude by 3'W longitude, we estimated the post-implementation change in risk of lethal vessel strikes. Estimates of vessel-operator voluntary compliance ranged from 57% to 87% and stabilized at 71% within the first 5 months of implementation. Our estimates showed an 82% reduction in the risk of lethal vessel strikes to right whales due to vessel-operator compliance. We conclude that the high level of compliance achieved with this voluntary conservation initiative occurred because the area to be avoided was adopted by the International Maritime Organization. Our results demonstrate that international shipping interests are able and willing to voluntarily alter course to protect endangered whales.

Scaling in Free-Swimming Fish and Implications for Measuring Size-at-Time in the Wild.

This study was motivated by the need to measure size-at-age, and thus growth rate, in fish in the wild. We postulated that this could be achieved using accelerometer tags based first on early isometric scaling models that hypothesize that similar animals should move at the same speed with a stroke frequency that scales with length-1, and second on observations that the speed of primarily air-breathing free-swimming animals, presumably swimming 'efficiently', is independent of size, confirming that stroke frequency scales as length-1. However, such scaling relations between size and swimming parameters for fish remain mostly theoretical. Based on free-swimming saithe and sturgeon tagged with accelerometers, we introduce a species-specific scaling relationship between dominant tail beat frequency (TBF) and fork length. Dominant TBF was proportional to length-1 (r2 = 0.73, n = 40), and estimated swimming speed within species was independent of length. Similar scaling relations accrued in relation to body mass-0.29. We demonstrate that the dominant TBF can be used to estimate size-at-time and that accelerometer tags with onboard processing may be able to provide size-at-time estimates among free-swimming fish and thus the estimation of growth rate (change in size-at-time) in the wild.

Genetic structure of capelin (Mallotus villosus) in the northwest Atlantic Ocean.

Capelin (Mallotus villosus) is a commercially exploited, key forage-fish species found in the boreal waters of the North Pacific and North Atlantic Oceans. We examined the population structure of capelin throughout their range in the Canadian northwest Atlantic Ocean using genetic-based methods. Capelin collected at ten beach and five demersal spawning locations over the period 2002 through 2008 (N = 3,433 fish) were genotyped using six polymorphic microsatellite loci. Temporally distinct samples were identified at three beach spawning locations: Chance Cove, Little Lawn and Straitsview, Newfoundland. Four capelin stocks are assumed for fisheries management in the northwest Atlantic Ocean based on meristics, morphometrics, tag returns, and seasonal distribution patterns. Our results suggested groupings that were somewhat different than the assumed structure, and indicate at least seven genetically defined populations arising from two ancestral populations. The spatial mosaic of capelin from each of the two basal cluster groups explains much of the observed geographic variability amongst neighbouring samples. The genetic-defined populations were resolved at Jost's Dest ≥ 0.01 and were composed of fish collected 1) in the Gulf of St. Lawrence, 2) along the south and east coasts of Newfoundland, 3) along coastal northern Newfoundland and southern Labrador, 4) along coastal northern Labrador, 5) near the Saguenay River, and at two nearshore demersal spawning sites, 6) one at Grebes Nest off Bellevue Beach on the east coast of Newfoundland, and 7) one off the coast of Labrador at Domino Run. Moreover, the offshore demersal spawners on the Scotian Shelf and Southeast Shoal appeared to be related to the inshore demersal spawners at Grebes Nest and in Domino Run and to beach spawners from the Gulf of St. Lawrence.

Genomic islands of divergence and their consequences for the resolution of spatial structure in an exploited marine fish.

As populations diverge, genomic regions associated with adaptation display elevated differentiation. These genomic islands of adaptive divergence can inform conservation efforts in exploited species, by refining the delineation of management units, and providing genomic tools for more precise and effective population monitoring and the successful assignment of individuals and products. We explored heterogeneity in genomic divergence and its impact on the resolution of spatial population structure in exploited populations of Atlantic cod, Gadus morhua, using genome wide expressed sequence derived single nucleotide polymorphisms in 466 individuals sampled across the range. Outlier tests identified elevated divergence at 5.2% of SNPs, consistent with directional selection in one-third of linkage groups. Genomic regions of elevated divergence ranged in size from a single position to several cM. Structuring at neutral loci was associated with geographic features, whereas outlier SNPs revealed genetic discontinuities in both the eastern and western Atlantic. This fine-scale geographic differentiation enhanced assignment to region of origin, and through the identification of adaptive diversity, fundamentally changes how these populations should be conserved. This work demonstrates the utility of genome scans for adaptive divergence in the delineation of stock structure, the traceability of individuals and products, and ultimately a role for population genomics in fisheries conservation.