RESUMO
An example of zeolite selectivity (MFI â MOR) driven by synthesis aging time has been studied. Using N,N,N',N'-tetramethyl-N,N'-dipropyl-ethylenediammonium diiodide (TMDP) as an organic structure-directing agent (OSDA), the zeolite phases obtained at 2 h (MFI 97%), 8 h (MFI 84%, MOR 16%) and 24 h (MFI 43%, MOR 57%) have been characterized by powder X-ray diffraction. The results suggest that at intermediate aging time, namely 8 h and 24 h, the dominant phase (MFI) is displaced by MOR. Different techniques (FT-IR, Raman, 13C MAS NMR, TGA/DTG and HC microanalysis) have been employed to verify the OSDA integrity and occlusion inside the zeolite micropores as well as to quantify the water and OSDA loading. The 1H MAS NMR of the as-made occluded zeolite was compared with the spectra of TMDP and the recovered OSDA from the sample by extraction with water. The comparison indicated that TMDP was not structurally intact, indicating the chemical transformation of TMDP to imidazolinium homologues through the Hofmann degradation process. Furthermore, careful acidic breakdown of the aluminosilicate shell, covered on the zeolite samples by hydrofluoric acid, revealed that the remaining OSDA had been partially degraded to lower molecular weight ammonium salt, confirmed by 1H NMR and mass spectrometry measurements. A computational study was performed by using a force field based methodology, including accurate loading of water and OSDA in the zeolite (MFI and MOR) unit cells. The results show an important contribution of the presence of water. The samples with larger aging time (8 h and 24 h) incorporate less water and show partial TMDP degradation, whilst at the shortest aging time (2 h), there is a larger water content and TMDP remains intact. The larger accessible volume of MFI justifies that this is the dominant phase at short aging times (large water content) since it can accommodate a larger number of water molecules than MOR. The OSDA partial degradation also plays a role. At longer aging times the partial OSDA decomposition has been considered in the models by including TMDP + Imidaz, which is more stabilized by MOR, whilst at shorter aging times the only OSDA present, TMDP, is better stabilized by MFI.
RESUMO
Management of superficial aspergillosis is a major challenge owing to the frequent relapses and treatment failure, which may pose a potential risk, thereby gradually developing resistant species. Therefore, necessitating the development of new antifungals with higher potency should be considered as alternative strategies for efficient management of infections. We aimed to investigate the susceptibility of Aspergillus isolates toward a novel triazole, efinaconazole, in comparison with various classes of antifungal drugs. Antifungal susceptibility testing was performed according to the Clinical and Laboratory Standards Institute M38-A2 guidelines. Efinaconazole exhibited poor activity against mutant A. fumigatus strains, A. niger sensu stricto, and A. tubingensis with GM MIC values of 3.62, 1.62, and 2 µg/ml, respectively; however, surprisingly, it efficiently inhibited the growth of A. terreus sensu stricto, followed by wild-type A. fumigatus and A. flavus with GM MIC values of 0.29, 0.42, and 0.52 µg/ml, respectively. Presumably, efinaconazole is inefficient in aspergillosis treatment due to the low susceptibility of A. niger sensu stricto, A. tubingensis, and mutant A. fumigatus; however, it may be effective in treating superficial aspergillosis caused by wild-type A. fumigatus, A. terreus sensu stricto, and A. flavus. Further studies are needed to determine how these findings may translate into in vivo efficacy.
Assuntos
Aspergillus/isolamento & purificação , Triazóis/farmacologia , Antifúngicos/farmacologia , Aspergilose/tratamento farmacológico , Aspergillus/efeitos dos fármacos , Testes de Sensibilidade MicrobianaRESUMO
Kinetic hydrate inhibitor laboratory testing before field application is one of the key priorities in the oil and gas industry. The common induction-time-based technique is often used to evaluate and screen for kinetic hydrate inhibitors (KHIs). However, the main challenge relates to the stochastic nature of hydrate nucleation observed in fresh systems, which often results in scattered data on hydrate formation with unacceptable uncertainties. A much more precise KHI evaluation method, called crystal growth inhibition (CGI), provides comprehensive insights into the inhibitory behavior of a kinetic hydrate inhibitor, including both hydrate formation and decomposition. Given that industry does not require this much information, it is not feasible to expend either much time or cash on this strategy. This study aims to provide a cost-effective technique that presents maximum data accuracy and precision with relatively little time and cost expenditure. Hence, the impact of water-hydrate memory on improving the accuracy and repeatability of the results of the induction-time-based technique (IT method) was examined. First, the concept of water-hydrate memory, which contains information about how it is created, was reviewed, and then, the factors influencing it were identified and experimentally investigated, like the heating rate of hydrate dissociation and the water-hydrate memory target temperature during heating. Finally, a procedure was developed based on the background information in the earlier sections to compare the consistency of the results, originating from the conjunction of water-hydrate memory with the IT technique. The results of replications at KHI evaluation target temperatures of 12.3-12.4°C and 11.5-11.7°C showed that more repeatable data were obtained by applying water-hydrate memory, and a more conclusive decision was made in evaluating KHI performance than with an IT method. It seems that combining the IT method with water-hydrate memory, introduced as the "HME method", can lead to more definitive evaluations of KHIs. This approach is expected to gain in popularity, even surpassing the accurate but complex and time-consuming CGI method.
RESUMO
A systematic change of HZSM-5 (HZ5) as a catalyst of the methanol to aromatics (MTA) reaction was undertaken by employing a fixed-bed tubular-type reactor under ambient pressure, applying a weight hourly space velocity (WHSV) of 2 h-1 at 375 °C, as the first report on the application of low-Si/Al-ratio alkaline-[Mo,Na]-HZSM-5 in the MTA process. To characterize the surface and textural properties of the catalysts, powder X-ray diffraction (PXRD), nitrogen adsorption/desorption, temperature-programmed desorption of ammonia (NH3-TPD), pyridine-infrared spectroscopy (Py-IR), thermogravimetric analysis (TGA), and energy-dispersive X-ray (EDX) methods were employed. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) measurements demonstrated a selectivity of up to 86 wt % (65.7 wt % for benzene, toluene, and xylene (BTX)) over 2[Mo]HZ5. NH3-TPD and Py-IR results indicated a sensible decrease of strong acid sites on the impregnated samples, while the surface analyses revealed the highest Lewis acid sites (LAS) together with the largest mesopore surface area for 2[Mo]alk-HZ5, supporting the migration of Mo species to the bulk of the catalysts. Mo impregnation had a minor effect on the observed coke formation in the promoted catalyst.
RESUMO
Two new macrocyclic ligands containing 17- and 19-membered O2N3-donor aza-crowns anchored to [60]Fullerene were synthesized and characterized by employing HPLC, electrospray ionization mass (ESI-MS), 1H and 13C NMR, UV-vis, IR spectroscopies, as well as powder X-ray diffraction (PXRD) and thermogravimetric analysis (TGA) in solid state. TGA measurements revealed that upon linking each of these macrocycle rings to [60]Fullerene, the decomposition point measured for [60]Fullerene moiety was increased, indicating on the promoted stability of [60]Fullerene backbone during binding to these macrocyclic ligands. Moreover, the ground state non-covalent interactions of [60]Fullerene derivatives of O2Nx (x=2, and 3) aza-crown macrocyclic ligands namely, L1-L4 with zinc phthalocyanine (ZnPc) were also investigated by UV-vis absorption, steady state and time resolved fluorescence spectrophotometry in N-methyl-2-pyrrolidone (NMP). The calculation of Stern-Volmer constants (KSV) indicated on existence of an efficient quenching mechanism comprising of the excited singlet state of ZnPc in the presence of L1-L4. The observation of an appropriate correlation between decrease in fluorescence intensity and lifetime parameters led us to propose the occurrence of a static mechanism for the fluorescence quenching of ZnPc in the presence of L1-L3. The binding constants (KBH) of L1-L4/ZnPc were also determined applying the fluorescence quenching experiments. Meanwhile, the incompatibility of both KSV and KBH values found for L4 was also described in terms of structural features using DFT calculations using the B3LYP functional and 6-31G* basis set.