Detalhe da pesquisa
1.
LC-HRMS-based targeted metabolomics for high-throughput and quantitative analysis of 21 growth inhibition-related metabolites in Chinese hamster ovary cell fed-batch cultures.
Biomed Chromatogr
; 36(5): e5348, 2022 May.
Artigo
em Inglês
| MEDLINE | ID: mdl-35083760
2.
Tailoring translational strength using Kozak sequence variants improves bispecific antibody assembly and reduces product-related impurities in CHO cells.
Biotechnol Bioeng
; 117(7): 1946-1960, 2020 07.
Artigo
em Inglês
| MEDLINE | ID: mdl-32246763
3.
FX knockout CHO hosts can express desired ratios of fucosylated or afucosylated antibodies with high titers and comparable product quality.
Biotechnol Bioeng
; 114(3): 632-644, 2017 03.
Artigo
em Inglês
| MEDLINE | ID: mdl-27666939
4.
Combining regulated and constitutive protein expression significantly boosts protein expression by increasing productivity without affecting CHO cell growth.
Biotechnol Prog
; 39(3): e3337, 2023.
Artigo
em Inglês
| MEDLINE | ID: mdl-36878664
5.
Activation of the PERK branch of the unfolded protein response during production reduces specific productivity in CHO cells via downregulation of PDGFRa and IRE1a signaling.
Biotechnol Prog
; 39(5): e3354, 2023.
Artigo
em Inglês
| MEDLINE | ID: mdl-37161726
6.
The role of GRASP65 in Golgi cisternal stacking and cell cycle progression.
Traffic
; 11(6): 827-42, 2010 Jun.
Artigo
em Inglês
| MEDLINE | ID: mdl-20214750
7.
Bax and Bak knockout apoptosis-resistant Chinese hamster ovary cell lines significantly improve culture viability and titer in intensified fed-batch culture process.
Biotechnol Prog
; 38(2): e3228, 2022 03.
Artigo
em Inglês
| MEDLINE | ID: mdl-34951158
8.
Expressing antigen binding fragments with high titers in a targeted integration CHO host by optimizing expression vector gene copy numbers and position: A case study.
Biotechnol Prog
; 38(6): e3290, 2022 11.
Artigo
em Inglês
| MEDLINE | ID: mdl-36537257
9.
A ribonucleoprotein-based decaplex CRISPR/Cas9 knockout strategy for CHO host engineering.
Biotechnol Prog
; 38(1): e3212, 2022 01.
Artigo
em Inglês
| MEDLINE | ID: mdl-34538022
10.
Preventing pyruvate kinase muscle expression in Chinese hamster ovary cells curbs lactogenic behavior by altering glycolysis, gating pyruvate generation, and increasing pyruvate flux into the TCA cycle.
Biotechnol Prog
; 37(5): e3193, 2021 09.
Artigo
em Inglês
| MEDLINE | ID: mdl-34288605
11.
Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost-effective method for expression of complex molecules.
Biotechnol J
; 16(4): e2000230, 2021 Apr.
Artigo
em Inglês
| MEDLINE | ID: mdl-33259700
12.
Direct selection of monoclonal phosphospecific antibodies without prior phosphoamino acid mapping.
J Biol Chem
; 284(31): 20791-5, 2009 Jul 31.
Artigo
em Inglês
| MEDLINE | ID: mdl-19473967
13.
Insulin degrading enzyme (IDE) expressed by Chinese hamster ovary (CHO) cells is responsible for degradation of insulin in culture media.
J Biotechnol
; 320: 44-49, 2020 Aug 20.
Artigo
em Inglês
| MEDLINE | ID: mdl-32526262
14.
UBR E3 ligases and the PDIA3 protease control degradation of unfolded antibody heavy chain by ERAD.
J Cell Biol
; 219(7)2020 07 06.
Artigo
em Inglês
| MEDLINE | ID: mdl-32558906
15.
Endothelial intercellular cell adhesion molecule 1 contributes to cell aggregate formation in CHO cells cultured in serum-free media.
Biotechnol Prog
; 36(3): e2951, 2020 05.
Artigo
em Inglês
| MEDLINE | ID: mdl-31846224
16.
DjA1 maintains Golgi integrity via interaction with GRASP65.
Mol Biol Cell
; 30(4): 478-490, 2019 02 15.
Artigo
em Inglês
| MEDLINE | ID: mdl-30566031
17.
Utilizing a regulated targeted integration cell line development approach to systematically investigate what makes an antibody difficult to express.
Biotechnol Prog
; 35(2): e2772, 2019 03.
Artigo
em Inglês
| MEDLINE | ID: mdl-30592567
18.
Pyruvate Kinase Muscle-1 Expression Appears to Drive Lactogenic Behavior in CHO Cell Lines, Triggering Lower Viability and Productivity: A Case Study.
Biotechnol J
; 14(4): e1800332, 2019 Apr.
Artigo
em Inglês
| MEDLINE | ID: mdl-30179303
19.
Supplementation of Nucleosides During Selection can Reduce Sequence Variant Levels in CHO Cells Using GS/MSX Selection System.
Biotechnol J
; 13(1)2018 Jan.
Artigo
em Inglês
| MEDLINE | ID: mdl-28745430
20.
Beating the odds: The poisson distribution of all input cells during limiting dilution grossly underestimates whether a cell line is clonally-derived or not.
Biotechnol Prog
; 34(3): 559-569, 2018 05.
Artigo
em Inglês
| MEDLINE | ID: mdl-28941017