Diesel degradation capability and environmental robustness of strain Pseudomonas aeruginosa WS02.

Petroleum hydrocarbon (PHC) degrading bacteria have been frequently discovered. However, in practical application, a single species of PHC degrading bacterium with weak competitiveness may face environmental pressure and competitive exclusion due to the interspecific competition between petroleum-degrading bacteria as well as indigenous microbiota in soil, leading to a reduced efficacy or even malfunction. In this study, the diesel degradation ability and environmental robustness of an endophytic strain Pseudomonas aeruginosa WS02, were investigated. The results show that the cell membrane surface of WS02 was highly hydrophobic, and the strain secreted glycolipid surfactants. Genetic analysis results revealed that WS02 contained multiple metabolic systems and PHC degradation-related genes, indicating that this strain theoretically possesses the capability of oxidizing both alkanes and aromatic hydrocarbons. Gene annotation also showed many targets which coded for heavy metal resistant and metal transporter proteins. The gene annotation-based inference was confirmed by the experimental results: GC-MS analysis revealed that short chain PHCs (C10-C14) were completely degraded, and the degradation of PHCs ranging from C15-C22 were above 90% after 14 d in diesel-exposed culture; Heavy metal (Mn2+, Pb2+ and Zn2+) exposure was found to affect the growth of WS02 to some extent, but not its ability to degrade diesel, and the degradation efficiency was still maintained at 39-59%. WS02 also showed a environmental robustness along with PHC-degradation performance in the co-culture system with other bacterial strains as well as in the co-cultured system with the indigenous microbiota in soil fluid extracted from a PHC-contaminated site. It can be concluded that the broad-spectrum diesel degradation efficacy and great environmental robustness give P. aeruginosa WS02 great potential for application in the remediation of PHC-contaminated soil.

Transport and retention of n-hexadecane in cadmium-/naphthalene-contaminated calcareous soil sampled in a karst area.

Studying the transport of petroleum hydrocarbons in cadmium-/naphthalene-contaminated calcareous soils is crucial to comprehensive assessment of environmental risks and developing appropriate strategies to remediate petroleum hydrocarbons pollution in karst areas. In this study, n-hexadecane was selected as a model petroleum hydrocarbon. Batch experiments were conducted to explore the adsorption behavior of n-hexadecane on cadmium-/naphthalene-contaminated calcareous soils at various pH, and column experiments were performed to investigate the transport and retention of n-hexadecane under various flow velocity. The results showed that Freundlich model better described the adsorption behavior of n-hexadecane in all cases (R2 > 0.9). Under the condition of pH = 5, it was advantageous for soil samples to adsorb more n-hexadecane, and the maximum adsorption content followed the order of: cadmium/naphthalene-contaminated > uncontaminated soils. The transport of n-hexadecane in cadmium/naphthalene-contaminated soils at various flow velocity was well described by two kinetic sites model of Hydrus-1D with R2 > 0.9. Due to the increased electrostatic repulsion between n-hexadecane and soil particles, n-hexadecane was more easily able to breakthrough cadmium/naphthalene-contaminated soils. Compared to low flow velocity (1 mL/min), a higher concentration of n-hexadecane was determined at high flow velocity, with 67, 63, and 45% n-hexadecane in effluent from cadmium-contaminated soils, naphthalene-contaminated soils, and uncontaminated soils, respectively. These findings have important implications for the government of groundwater in calcareous soils from karst areas.

Emerging Pollutants - Part I: Occurrence, Fate and Transport.

Emerging Pollutants-Part I: Occurrence, Fate, and Transport is a sequel of Emerging Pollutants (EPs). The aims of this paper is to collect the relevant literature published in 2016 worldwide and analyze the knowledge and technologies developed in the aspects of screening, monitoring, occurrence, transport, fate, environmental toxicity and management of EPs that are relevant to water and wastewater.

Effect of competitive adsorption on zinc removal from aqueous solution and zinc smelting effluent by eucalyptus leaf-based magnetic biosorbent.

The objectives of this study were to investigate the competitive sorption behaviors and mechanisms of heavy metals onto ELMB, a novel eucalyptus-leaf-based magnetic biosorbent, and to study the potential application of ELMB in the treatment of actual zinc smelting effluent after a necessary pretreatment process. ELMB and ELMB-metals systems were characterized using several techniques. Competitive sorption of Zn2+ with Pb2+, Cu2+ and Cd2+ onto ELMB was studied by batch experiments and the used sorbent was separated under a magnetic field. The results show that the ELMB can be considered as paramagnetic material with various functional groups on its surface. The presence of Pb2+, Cu2+ and Cd2+ significantly decreases the sorption of Zn2+ in either the binary system or multimetal systems. The order of adsorption preference is Pb2+ > Cu2+ > Cd2+ > Zn2+ in multimetal systems and the sequence of competitive ability to zinc is: Pb2+ > Cu2+ > Cd2+. Non-competitive Langmuir multicomponent isotherm model fits to the adsorption data of Pb2+ and Cu2+ well in aqueous solution. The co-existent Ca2+ and SO42- decrease the removal efficiencies of heavy metals while the presence of Na+ and Cl- shows little effect in the multimetal solution. In the case of actual zinc smelting effluent, "pretreatment + ELMB sorption" is successfully applied to remove heavy metals and the contents of Zn2+ and its associated metals are well below discharge limits.

In situ phytoextraction of Mn and NH4+-N from aqueous electrolytic manganese residue solution by Pistia stratiotes: Effects of Fe/Co presence and rhizospheric microbe synergistic involvement.

The electrolytic manganese industry produces a large amount of electrolytic manganese residue (EMR). Soluble Mn, NH4+-N, and other pollutants may be released from the open-air stacked EMR and transported to the environment along with rainfall or surface runoff. Aqueous EMR solution (AES) generally contains various elements required for plant growth, and phytoremediation can be applied to remove these pollutants from AES. Since the contents of Fe and Co vary greatly in AES depending on the ore sources as well as the pre-treatment processes, the presence of bioavailable Fe and Co at different levels may affect plant growth, the rhizosphere microbes, and pollutant removal. The present study investigated the in-situ removal of Mn(II) and NH4+-N from AES solution using free floating aquatic plant Pistia stratiotes, focusing especially on the effects of Fe/Co presence and rhizospheric microbe synergistic involvement on contaminant removal. The results showed that 69.08% of Mn and 94.99% of NH4+-N were removed by P. stratiotes in 24 d. Both the presence of Fe(II) and Co(II) facilitated the Mn(II) immobilization and increased Mn(II) removal by 19-31% due to the enhanced peroxidase activity and the increased Mn accumulating in roots The complete removal of Mn from AES was found in the presence of Fe(II) at 2 mg L-1 or Co(II) at 0.5 mg L-1 and more than 51% accumulated Mn in the roots was stored in the vacuole and cytoplasm. BioMnOx was found on the surface of the roots, revealing that rhizofiltration, rhizospheric plaque/biofilm formation, and Mn biogeochemical cycle exert synergic effects on Mn(II) immobilization. The findings of the present study demonstrate the feasibility of using P. stratiotes in the treatment of aqueous EMR solutions and the presence of an appropriate amount of bio-available Fe and Co can promote the removal of Mn(II) and NH4+-N.

[Indirect determination of Cr(III) and Cr(VI) in milks by hydride generation-fluorescence spectrometry].

In the medium of 3 mol x L(-1) hydrochloric acid, Cr(VI) oxidized As(III) to be As(V), the As(III) content, which was left over, was determined by hydride generation-fluorescence spectrometry, giving that it was inversely proportional to Cr(VI) content. Based on the same principle and combined with potassium permanganate oxidation of Cr(III) to be Cr(VI), the Cr(III) was, therefore, determined. The sample digestion procedures and co-existing ions which might have interference were investigated. The instrumental operation parameters, reaction acidity and other influence factors were studied. Under optimum operation conditions, a good linear relationship was obtained in the range of 4.0-20 microg x L(-1) Cr. The detection limit of the method was 2.5 microg x L(-1). When the method was applied to milk analysis, a relative standard deviation (RSD, n = 6) of 1.6%-2.7% was found. The recovery was 96.5%-104.2%.

[Indirect determination of iodine with methyl isobutyl ketone (MIBK) extraction by atomic fluorescence spectrometer].

In this paper, a new method was developed for indirect determination of iodine with methyl isobutyl ketone (MIBK) extraction by atomic fluorescence spectrometer (AFS). Based upon the complex, which can be extacted perfectly by MIBK, formed between I- and Hg2+ in 0.1 mol x L(-1) of nitric acid, indirectly measuring the iodine in organic phase becomes possible. The effects of extraction conditions and other influence factors were investigated. Under the optimized experimental conditions, the results showed that the linearity between its absorbance and the concentration of iodine was 0-12 microg x L(-1), the detection limit is 0.14 microg x L(-1), and the relative standard deviation is 3.3%. The method has been applied to the determination of iodine in eggs, and the recoveries are in the range of 101.8%-110.4%.

[Butanol extraction combined with dilute hydrochloric acid dissolution-atomic fluorescence spectrometric method for indirect determination of molybdenum in Chinese herbal medicine].

A method for indirectly determining the molybdenum in Chinese herbal medicine by butanol extraction and dilute hydrochloric acid dissolution was established for atomic fluorescence spectrometry. The molybdoarsenate heteropoly acid, formed in the presence of As(V) and ammonium molybdate in 0.3 mol x L(-1) sulphuric acid medium, was separated and enriched in the organic solvent, then the evaporation of organic reagent was implemented and the left residue was dissolved in dilute hydrochloric acid in which the arsenic content was determined on behalf of molybdenum. In the optimum experimental conditions, molybdenum content in 0-15 microg x L(-1) range depicts a good linear relationship, the detection limit and relative standard deviation of 0.44 microg x L(-1) and 1.1% were obtained, respectively. Spiked Chinese herbal medicine samples were determined with the proposed method, and recoveries of 95.6%-101.3% were achieved.

A manganese-oxidizing bacterium-Enterobacter hormaechei strain DS02Eh01: Capabilities of Mn(II) immobilization, plant growth promotion and biofilm formation.

While biogenic Mn oxides (BioMnOx) generated by Mn(II)-oxidizing bacteria (MOB) have attracted increasing attention, a MOB strain isolated from Mn-polluted sediments was identified and assigned as Enterobacter hormaechei DS02Eh01. Its Mn(II) immobilization activity, plant growth-promoting traits, and biofilm formation capability were investigated. The results showed that strain DS02Eh01 was found to be able to tolerate Mn(II) up to 122 mM. The strain immobilized Mn(II) in aquatic media mainly through extracellular adsorption, bio-oxidation and pH-induced precipitation as well as manganese oxidation. DS02Eh01-derived BioMnOx are negatively charged and have a larger specific surface area (86.70 m2/g) compared to the previously reported BioMnOx. The strain can immobilize Mn(II) at extreme levels, for instance, when it was exposed to 20 mM Mn(II), about 59% of Mn(II) were found immobilized and 17% of Mn(II) were converted to MnOx. The SEM and TEM observation revealed that the DS02Eh01-derived BioMnOx were aggregates doped with granules and microbial pellets. The precipitated Mn(II) and the Mn(III)/Mn(IV) oxides co-existed in BioMnOx, in which Mn(II) and Mn(IV) were found dominant with Mn(II) accounting for 49.6% and Mn(IV) accounting for 41.3%. DS02Eh01 possesses plant growth-promoting traits and biofilm formation capacity even under Mn(II) exposure. Mn(II) exposure at 5 mM was found to stimulate strain DS02Eh01 to form biofilms, from which, the extracted EPS was mainly composed of aromatic proteins. This study reveals that E. hormaechei strain DS02Eh01 possesses the potential in environmental ecoremediation via coupling processes of macrophytes extraction, biochemical immobilization and biosorption.

Coupling suspect and non-target analytical methods for screening organic contaminants of concern in agricultural & urban impacted waters: Optimization and application.

Long-term exposure of contaminants to emerging concern (CECs) may pose risks to human health and ecosystems, even at low concentrations. Rivers impacted by both agricultural and urban activities experience distinctive environmental pressures due to receiving wastewaters that contain complex organics and their transformation products (TPs). In this study, we developed a regional database composed of 1200 CECs of high concern in Guangxi (South China). Further, we optimized a comprehensive analytical method for simultaneously screening for CECs and their TPs. The optimized screening method was applied to surface waters sampled from 10 different cross sections of a river that is impacted by both agricultural and urban activities. The best results of method optimization were achieved when the screening detection limit (SDL) ranged from 0.05 to 2 ng L-1, and over 90% of the analytes had acceptable recovery rates ranging between 64.7% and 95.6% (RSD < 11%). Of the 1200 CECs contained in the regional database, 168 were detected in at least one sampling site of the studied river via suspect screening, and among them, 36 contaminants were found at all sampling sites. Also, 58 additional contaminants and 39 TPs were tentatively identified via non-target screening, among which 4 TPs were reported for the first time in the aquatic environment. Triazine herbicides and their TPs were identified at most of the sampling sites, with ametryn and atrazine posing relatively high risks in the river ecosystems. Furthermore, 31 known analytes were selected as standards in order to confirm the combined screening method; one false positive occurred in the non-target screening method. According to these results, the suspect screening strategy provides valuable confirmation for the identification of a wide range of CECs in water, while non-target screening can provide a reference for researchers and supplement the regional database, particularly in the study of TPs.

Chemical behaviors and toxic effects of ametryn during the UV/chlorine process.

Ametryn (AMT), one of the most widely used herbicides in agriculture, has been frequently detected as a micropollutant in many aquatic environments. AMT residue not only pollutes water but also acts as a precursor for the production of disinfection by-products (DBPs). This study systematically investigated the fate of AMT during the UV/chlorine process. It was observed that the combination of UV irradiation and chlorination degraded AMT synergistically. The results of the radical quenching experiments suggested that AMT degradation by the UV/chlorine process involved the participation of UV photolysis, hydroxyl radical (OH) reactions, and reactive chlorine species (RCS) reactions, which accounted for 45.4%, 36.4%, and 14.5% of the degradation, respectively. Moreover, we found that Cl- 2 was an important reactive radical for AMT degradation. The chlorine dose, pH, coexisting anions (Cl- and HCO3-), and natural organic matter (NOM) were found to affect AMT degradation during the UV/chlorine process. Nineteen predominant intermediates/products of AMT degradation during UV/chlorine process were identified, including atrazine. Moreover, the corresponding transformation pathways were proposed, including electron transfer, bond cleavage (C-S, C-N), radical (OH, Cl and Cl- 2) reactions, and subsequent hydroxylation. The toxicity tests with Vibrio fischeri on AMT degradation suggested that more DBPs were generated by UV/chlorine-treated AMT, which possessed higher acute toxicity than AMT did. Although the UV/chlorine process evidently promoted the AMT degradation, optimization of process parameters may reduce the DBP production and merits further investigation.

Significance of manganese resistant bacillus cereus strain WSE01 as a bioinoculant for promotion of plant growth and manganese accumulation in Myriophyllum verticillatum.

Endophytic bacteria are generally helpful for plant growth and protection. Strain WSE01, which was identified as bacillus cereus, was isolated from the stem of Myriophyllum verticillatum and it displayed a high tolerance to Mn (1500 mg/L). The strain was found to be able to produce indole-3-acetic acid (IAA) and siderophores, fix the atmospheric nitrogen and dissolve potassium from insoluble K-bearing minerals. In hydroponic culture experiments, the inoculation of strain WSE01 significantly promoted the growth and increased the leaf enzyme activity in the inoculated plant M. verticillatum. Furthermore, the manganese content was increased by 36.4% in stems and by 54.7% in leaves of the inoculated plant under Mn stress at 400 mg/L, compared to the non-inoculated group. This study suggests that the strain WSE01 has the potential to be used as biocontrol and/or biofertilizing agents for application in macrophyte M. verticillatum and conduces to achieving more effective phytoremediation of metal-contaminated waters.

Contaminants of emerging concern in aquatic environment: Occurrence, monitoring, fate, and risk assessment.

The present work provides a review focusing on contaminants of emerging concern (CECs) in aquatic environment, with an emphasis on their occurrence, monitoring, fate, and risk assessment in the research published in the scientific literature in 2019. Several studies revealed that these organic contaminants were detected in many water bodies and suspect, nontarget, and target screening provided an efficient detection for the co-existing organic substances with complex components. Wastewater resource recovery facilities were concurrently considered as a central source, and several specific chemicals have been found to be used as chemical markers to track the source of CECs in some urban watersheds. Reliable monitoring, reliable fate/toxicity assessment, and effective removal that consider CECs as a heterogeneous group rather than single substances will be the challenges for the research community in the future.

Characteristics and disinfection byproducts formation potential of dissolved organic matter released from fast-growing Eucalyptus urophylla leaves.

Every year, the harvesting of Eucalyptus generates a large amount of abandoned Eucalyptus leaves (ELs), which may release dissolved organic matter (DOM) when immersed in water. If these substances are carried by surface runoff directly to the source of drinking water, some components in the DOM tend to form disinfection byproducts (DBPs) within the water-supply system, posing risk to human health. In this study, the characteristics of DOM released from leaves of Eucalyptus urophylla were studied and the potential of DBPs formation of the EL-released DOM during the chlorination process was investigated. The results showed that the EL-released DOM was mainly composed of small molecules and hydrophobic substances. Of the total EL-released DOM, the proportion with molecular weight less than 10 kDa accounted for over 80% and the hydrophobic substances took up over 62%. The DOM showed strong absorbance at UV254 and the fluorescence response corresponding to humic acid-like (HA-like) fractions, soluble microbial byproduct-like, aromatic protein and fulvic acid-like (FA-like) material, which have been considered to be related to the potential precursors of chlorinated DBPs. Non-targeted screening demonstrated the presence of phenolics, carbohydrates, and amino acids. The analysis of products generated in chlorination process revealed the formation of trichloromethane (TCM) and the total organic halogen (TOX). The present study fully confirms that the DOM released from Eucalyptus urophylla leaves has great potential for the generation of chlorinated DBPs.

Nitrofurazone degradation in the self-biased bio-photoelectrochemical system: g-C3N4/CdS photocathode characterization, degradation performance, mechanism and pathways.

In this study, a self-biased bio-photoelectrochemical system (SB-BPES) was constructed using a bioanode and the g-C3N4/CdS heterojunction photocathode for nitrofurazone (NFZ) degradation under solar irradiation. The physio-chemical properties and optical performance of photocatalysts were characterized, and photo-electrochemical properties of various photocathodes were analyzed. Results showed that g-C3N4/CdS exhibited the broadest visible light absorption range (to 594 nm) and the most efficient e--h+ separation; and its corresponding photocathode showed the highest photocurrent (9.8 µA), and the lowest charge transfer resistance (5.43 ☓ 103 Ω). In the solar-illuminated SB-BPES with g-C3N4/CdS photocathode, about 80% of NFZ removal rate was achieved within 10 h. More importantly, TOC removal of 62.6% was achieved in 24 h, which was 1.8 times of that from the open circuit SB-BPES, and 4.3 folds of that from microbial degradation; also, about 1.5 times of those from SB-BPES with g-C3N4 and CdS photocathodes. Besides, reproducible current generations (∼1.0 mA) were produced. These verified that it was a self-sustained system for spontaneously pollutants degradation and electricity generation. Moreover, possible degradation mechanism and pathways were proposed according to the identified intermediates. This study provides inspiration for synchronic improving refractory organics degradation and net energy recovery.

Emerging pollutants in water environment: Occurrence, monitoring, fate, and risk assessment.

The occurrence of emerging pollutants (EPs) is continuously reported worldwide. Nevertheless, only few of these compounds are toxicologically evaluated due to their vast numbers. Reliable analytical methods and toxicity assessment methods are the basis of either the management or the elimination of EPs. In this paper, literature published in 2018 on EPs were reviewed with special regard to their occurrence, detection methods, fate in the environment, and ecological toxicity assessment. Particular focus was placed on practical considerations, novel processes, and new solution strategies. PRACTITIONER POINTS: Literature published in 2018 on emerging pollutants were reviewed. This review article is with special regard to the occurrence, detection methods, fate and toxicity assessment of emerging pollutants. Particular focus was placed on practical considerations, novel processes and new solution strategies.

A novel manganese oxidizing bacterium-Aeromonas hydrophila strain DS02: Mn(II) oxidization and biogenic Mn oxides generation.

The extensive applications of biogenic manganese oxides (BioMnOx) generated by manganese oxidizing bacteria (MOB) have attracted considerable attentions. In this study, we report on a novel MOB that has been isolated from sediments and identified as Aeromonas hydrophila strain DS02. The Mn(II) oxidation activity of strain DS02 under Mn(II) stress and the application of the associated BioMnOx products were investigated. Nearly 90.0% (495 mg L-1) of the soluble Mn(II) were removed and 45.6% (240 mg L-1) was converted to Mn(III/IV). Fitting the XPS data showed that Mn(IV)-oxide is the major component (82.0%) of the flake-shaped BioMnOx, corresponding to an average Mn oxidation number of 3.71. When the BioMnOx were coupled with the PMS activation, a 99.5% catalytic degradation of 2,4-dimethylaniline was observed after 80 min, revealing a high degradation efficiency.

Occurrence of microcystin-LR in vegetated lagoons used for urban runoff management.

Phytoremediation with aquatic macrophyte has been considered as an eco-friendly technique for controlling harmful cyanobacteria outbreak and proven to be effective. The conventional water quality parameters are frequently measured to evaluate the effectiveness of phytoremediation. However, the concentration of microcystin-leucine-arginine (MC-LR) in different vegetated water still remains uncertain. In this study, the contents of MC-LR in four macrophyte-vegetated lagoons were determined by solid phase extraction and ultra-high-performance liquid chromatography with tandem mass spectrometry technology. Results indicated that MC-LR was found in Nymphaea tetragona lagoon (lagoon-S), Vallisneria spiralis lagoon (lagoon-B) and another Vallisneria spiralis lagoon (lagoon-J). Only in lagoon dominated by Pistia stratiotes L. (lagoon-D), MC-LR concentration was undiscovered regardless of seasonal variation. The levels of MC-LR varied seasonally and were affected by the different vegetated aquatic macrophytes. The results suggest that in addition to conventional physicochemical parameters and indicators of water quality, MC-LR levels should be taken into consideration when the effectiveness of phytoremediation is assessed.

Regeneration of magnetic biochar derived from eucalyptus leaf residue for lead(II) removal.

Regeneration of Pb-loaded magnetic biochar prepared with eucalypts leaf residue was studied by using EDTA-2Na as a regenerant. The desorption efficiency was found to be 84.1% in 120 min with iron leaching amount of 1.1 mg g(-1). Higher SBET and pore volume were observed in regenerated magnetic biochars and no significant band shifts occurred in FTIR spectra during 6 regeneration cycles. The decrease of Pb(II) adsorption capacity (from 52.4 to 41.5 mg g(-1)) was only found in the first regeneration cycle. Magnetic separation performance of adsorbents was not significantly affected by multiple regeneration cycles.

Combined performance of biochar sorption and magnetic separation processes for treatment of chromium-contained electroplating wastewater.

Magnetic biochar was prepared with eucalyptus leaf residue remained after essential oil being extracted. Batch experiments were conducted to examine the capacity of the magnetic biochar to remove Cr (VI) from electroplating wastewater and to be separated by an external magnetic field. The results show that the initial solution pH plays an important role on both sorption and separation. The removal rates of Cr (VI), total Cr, Cu (II), and Ni (II) were 97.11%, 97.63%, 100% and 100%, respectively. The turbidity of the sorption-treated solution was reduced to 21.8NTU from 4075NTU after 10min magnetic separation. The study also confirms that the magnetic biochar still retains the original magnetic separation performance after the sorption process.