The stress granule protein G3BP1 alleviates spinocerebellar ataxia-associated deficits.

Polyglutamine diseases are a group of neurodegenerative disorders caused by an abnormal expansion of CAG repeat tracts in the codifying regions of nine, otherwise unrelated, genes. While the protein products of these genes are suggested to play diverse cellular roles, the pathogenic mutant proteins bearing an expanded polyglutamine sequence share a tendency to self-assemble, aggregate and engage in abnormal molecular interactions. Understanding the shared paths that link polyglutamine protein expansion to the nervous system dysfunction and the degeneration that takes place in these disorders is instrumental to the identification of targets for therapeutic intervention. Among polyglutamine diseases, spinocerebellar ataxias (SCAs) share many common aspects, including the fact that they involve dysfunction of the cerebellum, resulting in ataxia. Our work aimed at exploring a putative new therapeutic target for the two forms of SCA with higher worldwide prevalence, SCA type 2 (SCA2) and type 3 (SCA3), which are caused by expanded forms of ataxin-2 (ATXN2) and ataxin-3 (ATXN3), respectively. The pathophysiology of polyglutamine diseases has been described to involve an inability to properly respond to cell stress. We evaluated the ability of GTPase-activating protein-binding protein 1 (G3BP1), an RNA-binding protein involved in RNA metabolism regulation and stress responses, to counteract SCA2 and SCA3 pathology, using both in vitro and in vivo disease models. Our results indicate that G3BP1 overexpression in cell models leads to a reduction of ATXN2 and ATXN3 aggregation, associated with a decrease in protein expression. This protective effect of G3BP1 against polyglutamine protein aggregation was reinforced by the fact that silencing G3bp1 in the mouse brain increases human expanded ATXN2 and ATXN3 aggregation. Moreover, a decrease of G3BP1 levels was detected in cells derived from patients with SCA2 and SCA3, suggesting that G3BP1 function is compromised in the context of these diseases. In lentiviral mouse models of SCA2 and SCA3, G3BP1 overexpression not only decreased protein aggregation but also contributed to the preservation of neuronal cells. Finally, in an SCA3 transgenic mouse model with a severe ataxic phenotype, G3BP1 lentiviral delivery to the cerebellum led to amelioration of several motor behavioural deficits. Overall, our results indicate that a decrease in G3BP1 levels may be a contributing factor to SCA2 and SCA3 pathophysiology, and that administration of this protein through viral vector-mediated delivery may constitute a putative approach to therapy for these diseases, and possibly other polyglutamine disorders.

Giant benign lymphangioendothelioma with positive expression of Wilms tumor 1: A case report.

Benign lymphangioendothelioma (BL, acquired progressive lymphangioma) is a rare, slow-growing lymphatic tumor, first described 40 years ago, with fewer than 50 published cases. Clinically, it presents as a skin-colored or erythematous patch. Definitive diagnosis requires histopathological examination. The immunohistochemical staining profile is still controversial regarding Wilms tumor 1 (WT1) expression, a marker of proliferative and neoplastic, rather than malformative nature. Here, we report a case of a 60-cm-long BL on the breast of an adult female. Biopsy revealed irregular vascular spaces dissecting the collagen bundles lined by swollen endothelial cells but without cellular atypia. Positivity for podoplanin (D2-40), CD31, and WT1 was observed, supporting the neoplastic nature of this lesion. Dermatologists and pathologists must be aware of this entity for early diagnosis and treatment.

Set to fail: Affective dynamics in a resistance training program designed to reach muscle concentric failure.

Grounded in hedonic assumptions, evidence suggests that people tend to engage in activities they consider pleasurable and enjoyable, while trying to avoid pain and displeasure. This suggests that the dynamic between positive and negative affect can influence current behavior and the intentions to continue performing. Regarding resistance training (RT), research focusing on how to promote a better affective response is still scarce and much needed. Given existing limitations and theoretical suggestions, a RT program was developed and applied to recreational exercisers in a quasi-experimental design aiming to (1) explore the affective response dynamic through an assessment after the last set of each exercise; and (2) analyze possible differences of preference and tolerance profiles in affective variables (core affect and enjoyment). For that purpose, 43 participants (21 male and 22 female; Mage  = 34.69 ± 6.71 years; Mexperience  = 8.32 ± 4.54 years; MBMI  = 24.26 ± 2.64 kg/m2 ) accepted to participate in this study. Descriptive statistics, correlational, and group comparisons analyses were performed to provide evidence for proposed objectives. The present study showed that measures of affective valence/arousal applied immediately after a set represents a feasible and ecologically valid approach to tap core affect. Results presented evidence that recreationally trained exercisers in a common RT program would need a minimum of one measurement to assess the affective response. However, additional assessments could refine the understanding of exercise pleasurable experience. Results also suggest that exercisers with distinct profiles of preference/tolerance depicted differentiated patterns for the affective response, possibly justifying a distinct approach when promoting affective regulation.

Understanding the Associations across Fibromyalgia-Related Fatigue, Depression, Anxiety, Self-Esteem Satisfaction with Life and Physical Activity in Portuguese and Brazilian Patients: A Structural Equation Modeling Analysis.

Background and Objectives: Fibromyalgia are heterogeneous and differ from patient to patient; however, the most reported are general myalgia and at specific points associated with fatigue and certain psychological adversities. Physical activity can mitigate the effects of the symptoms. However, the associations between fibromyalgia-related fatigue, self-esteem, anxiety, depression, satisfaction with life and physical activity are unclear. Therefore, the aim of the present study was to understand the associations between these symptoms and whether there are differences between these associations across two distinct cultures. Materials and Methods: A total of 473 women aged between 28 and 75 years (M = 49.27; SD ± 8.28) completed five questionnaires about fibromyalgia-related fatigue, physical activity, anxiety, depression, self-esteem, and satisfaction with life. Results: Fibromyalgia-related fatigue was positively associated with depression and anxiety, depression and anxiety were negatively associated with self-esteem, self-esteem was positively associated with satisfaction with life, satisfaction with life was positively associated with physical activity and there were no differences in terms of the perceptions and associations of these variables between Portuguese and Brazilian patients. Conclusions: Our results showed the significant role of associations between these variables and a similarity in the perception and relationship of the variables between the two cultures.

Trainer-exerciser relationship: The congruency effect on exerciser psychological needs using response surface analysis.

Perceptions of fitness trainers' need-supportive and need-thwarting behaviors have been shown to impact exercisers' psychological need satisfaction and frustration. Currently, it is unknown whether an agreement or disagreement between exercisers' and fitness trainers' reported perceptions of these behaviors leads to the satisfaction and/or frustration of psychological needs. Based on self-determination theory, the present study examined the effect of congruency between fitness trainers' and exercisers' perceptions of need-supportive and need-thwarting interpersonal behaviors on basic psychological need satisfaction and frustration. A sample of 130 fitness trainers (43 females; Mage  = 31.58 ± 7.65) and a total of 640 gym exercisers (350 females; Mage  = 34.23 ± 11.59) participated in this study. Findings suggested that the majority of fitness trainers tended to over-report their use of need-supportive behavior and under-report their need-thwarting behaviors. Results showed that when there was congruency between fitness trainers' reported use and exercisers' perception of interpersonal behaviors, basic need satisfaction tended to increase. This effect was greater for exercisers that rated their respective fitness trainer high on relatedness support. Fitness trainers should be self-aware of their interpersonal behaviors when engaging with exercisers and interventions based on self-determination theory could serve as a promising avenue to improve the quality of exercisers' experience.

Differences between Portuguese and Brazilian Patients with Fibromyalgia Syndrome: Exploring the Associations across Age, Time of Diagnosis, and Fatigue-Related Symptoms.

Background and Objectives: The current literature demonstrates that different cultures have different perceptions of the symptoms of Fibromyalgia Syndrome (FM). The aim of the study was to explore the differences between Brazilian and Portuguese patients with FM in their fatigue experience and to measure the differences in the perception of fatigue according to age and duration of diagnosis. Materials and Methods: In total, 209 Portuguese women aged between 21 and 75 years old (M = 47.44; SD = 10.73) and 429 Brazilian women aged between 18 and 77 years old (M = 46.51; SD = 9.24) were recruited to participate in the present study. Participants filled out the items in the Multidimensional Daily Fatigue-Fibromyalgia-17 Diary (MDF-Fibro-17), a specific tool to measure the level of five components of FM-related fatigue. Results: The results showed a greater perception of all of the components of fatigue in the Brazilian sample. No significant differences were found related to the age and duration of FM diagnosis. Conclusions: Overall, there are significant differences in fatigue symptoms between Portuguese and Brazilian women with FM, suggesting that cultural and geographical differences should be considered when describing fatigue-related symptoms in women with FM.

The bright and dark sides of motivation as predictors of enjoyment, intention, and exercise persistence.

OBJECTIVE: The present study aimed to examine the bright and dark sides of motivation, based on self-determination theory, as predictors of enjoyment, intention to continue exercising, and exercise persistence. In addition, we analyzed the mediation role of each variable and tested the model invariance between male and female exercisers. METHODS: A total of 575 gym exercisers (female = 230) aged between 18 and 65 years (M = 34.07; SD = 11.47) participated in this study. All participants had at least 6 months of regular exercise practice; exercise frequency of the participants ranged from 2 to 7 sessions per week (M = 3.52; SD = 1.28) and training sessions lasted approximately 30 and 120 minutes (M = 61.54; SD = 17.64). Participants completed a multisection questionnaire assessing interpersonal behaviors, basic psychological needs satisfaction and frustration, behavioral regulations, enjoyment, and intentions to continue exercising. Exercise persistence was assessed 6 months after initial assessment using gym computer system attendance records. Several analyses, namely, confirmatory factor analysis, structural equation modeling, serial mediation analysis, and structural invariance testing, were performed to increase theoretical and empirical knowledge. RESULTS: Positive associations were observed among the bright side of motivation, enjoyment, intention to continue exercising, and persistence. On the other hand, the dark side of motivation was negatively associated with the bright side constructs, enjoyment, intention, and exercise persistence. Both measurement [χ2 (370) = 1496.708, χ2 /df = 4.05; B-S P < .001, CFI = 0.907, TLI = 0.901, SRMR = 0.056, RMSEA = 0.073 (CI 90% = 0.063, 0.083)] and structural models [χ2 (393) = 2484.994, χ2 /df = 6.323; B-S P < .001, CFI = 0.908, TLI = 0.900, SRMR = 0.075, RMSEA = 0.071 (CI 90% = 0.067, 0.075)] displayed acceptable fit to the data. The results also showed that basic psychological needs satisfaction, autonomous motivation, enjoyment, and intention to continue exercising were significant mediators. However, enjoyment had the highest effect on exercise persistence. In addition, structural model invariance was confirmed between male and female exercisers. CONCLUSION: Motivational determinants have implications for predicting emotional, cognitive, and behavior-related outcomes. Individuals should perceive exercise instructors as supportive and exercising as a fun activity to endorse long-term exercise persistence.

Exergames for Children and Adolescents with Autism Spectrum Disorder: An Overview.

Autistic Spectrum Disorder (ASD) is a complex neurodevelopmental disorder associated with various etiologies and characterized by deficits in social interaction, emotional reciprocity, communication, motor skills and cognitive functions. Studies have proposed that limited levels of physical activity and late motor skills and fitness, particularly in children and adolescents with ASD, may accentuate social and emotional deficits. In view of this, exergames, which are active video-games, can be considered a low-cost and safe type of exercise for children and adolescents with ASD, since they are more enjoyable than ordinary physical activities, influencing on treatment adherence. Thus, our study aims to evidence the effects of exergames on physical fitness, cognitive functions, and repetitive behaviors in children and adolescents with ASD. Despite the small number of studies investigating the effects of exergames as new strategy in children and adolescents with ASD, results suggest exergames as potential tool for the treatment of children and adolescents with ASD for improvement in physical fitness, cognitive functions and repetitive behavior. Our review pointed towards the importance of exergames for children and adolescents with ASD. Despite few studies conducted about this issue, we can consider exergames a potential tool to increase physical fitness, cognitive functions and to decrease repetitive behavior in children and adolescents with ASD. Moreover, health professionals should be careful when attempting to help this population, because the current literature is unclear yet about the improvement of ASD features through exergames.

Autosomal recessive hyper-IgE syndrome successfully treated with hematopoietic stem cell transplantation.

Autosomal recessive hyper-IgE syndrome is a primary immunodeficiency that results from a mutation in the DOCK8 gene. We report a case of a patient presenting with severe eczema, atopy, and recurrent skin infections since the first months of life. The diagnosis of autosomal recessive hyper-IgE syndrome was made at the age of 7 by a positive DOCK8 genetic test. The patient underwent hematopoietic stem cell transplantation, with complete remission of the various manifestations.

The role of dark-side of motivation and intention to continue in exercise: A self-determination theory approach.

Motivation plays an important role when it comes to regular physical exercise. Hence, low levels of intentions to continue exercising in the future may ultimately lead to higher rates of exercise dropout. The first objective of the present research was to test a theoretical model considering the dark side of motivational determinants on intentions towards exercising in the future. The second objective consisted in comparing groups with different characteristics, as a way to identify individuals with weaker intention, which is believed to be an indicator of a higher dropout risk. In total, 544 (294 female; 250 male) gym exercisers aged between 18 and 58 years (M = 35.00; SD = 11.57) partook in the present research. Participants were engaged in fitness group classes (n = 273) or in cardio/resistance workouts (n = 271), and their exercise experience ranged from 3 to 120 months (M = 47.41; SD = 7.54). Results showed that the measurement and the structural model had an excellent fit. In addition, the structural model was invariant between gender, fitness activities, and exercise experience. Each group displayed different explained variance in intention to continue exercising. Overall, male, young adults and more experienced exercisers had stronger intentions towards exercising. Fitness instructors should be aware of their own thwarting behaviors, especially when interacting with female, young and less experienced exercisers, since they have weaker intentions to exercise in the future, possibly representing an increased risk of withdrawal.

Feasibility and Usefulness of Repetitions-In-Reserve Scales for Selecting Exercise Intensity: A Scoping Review.

The intensity of resistance training (RT) exercise is an important consideration for determining relevant health and performance-related outcomes. Yet, current objective exercise intensity measures present concerns in terms of viability or cost. In response to these concerns, repetition-in-reserve (RIR) scales may represent an adequate method of measuring and regulating intensity. However, no recent review has focused on how RIR scales have been used for this purpose in prior research. We prepared the present scoping review to analyze the feasibility and usefulness of RIR scales in selecting RT intensity. We conducted a systematic search in PubMed, SPORTDiscus, PsycINFO, and ClinicalTrials.gov databases (last search date April 2023) for experimental and non-experimental studies that utilized an RIR scale to measure proximity to failure in RT activities with apparently healthy individuals of any age. We qualitatively analyzed 31 studies (N = 855 mostly male adult participants) published between 2012-2023. RIR scales appeared to be contextually feasible and useful in prescribing and adjusting RT intensity. The most common trend in this research was to prescribe a target RIR and adjust the exercise load for a desired proximity to muscle failure. Additionally, when measuring proximity to failure as an outcome of interest, the literature suggests that the RIR prediction should be made close to task failure to increase its accuracy. Future research should further explore the impact of sex, RT experience, exercise selection, and muscle conditioning on the overall RIR approach.

Corrigendum: Enjoyment as a predictor of exercise habit, intention to continue exercising, and exercise frequency: the intensity traits discrepancy moderation role.

[This corrects the article DOI: 10.3389/fpsyg.2022.780059.].

Validation of the Portuguese Version of the Perceived Physical Literacy Instrument.

BACKGROUND: The Perceived Physical Literacy Instrument (PPLI) has been the most adopted physical literacy instrument designed for adults to date, having been already translated to 5 languages. Still, despite its popularity, there is limited evidence of its psychometric properties. The aim of this study was to translate and adapt cross-culturally the PPLI to the Portuguese language and to analyze the psychometric properties of the Portuguese version in a sample of Portuguese-speaking adults. METHODS: The study sample was composed of 434 participants (70% females) with a median age of 25 (18-39) years. Confirmatory factor analysis was used to assess psychometric properties of the scale. In addition, instrument assumptions between sexes through invariance testing were examined. Finally, concurrent validity, test-retest reliability, and test-retest agreement analyses were performed to gather additional evidence. RESULTS: The results of the present study generally supported the measurement model. The 9-item, 3-factor model presented a good fit to the data and adequate construct validity (χ2 = 82.859 [24], P < .001, comparative-fit index = .948, Tucker-Lewis index = .922, standardized root mean square residual = .049, root mean square error of approximation = .075). In addition, the scale presented concurrent validity. Some issues arose with measurement invariance and overtime consistency. CONCLUSIONS: Considering the lack of validated instruments, we suggest a fair use of Portuguese version of PPLI to access physical literacy in adults. This study contributes to the validation of the PPLI by providing evidence of its psychometric properties in the Portuguese-speaking adult population and facilitates comparative analysis with previous and future studies utilizing the PPLI instrument.

Analysis of the Effect of Different Physical Exercise Protocols on Depression in Adults: Systematic Review and Meta-analysis of Randomized Controlled Trials.

CONTEXT: Physical exercise (PE) is an effective treatment for depression, alone or as an adjunct. OBJECTIVE: There is a lack of indicators regarding the frequency, intensity, duration, and type of physical exercise (PE). This study aims to synthesize and analyze the dose-effect of different PE protocols in adult subjects in the treatment of depression, based on the analysis of randomized controlled trials (RCTs). DATA SOURCES: The search was conducted using Web of Science, PubMed, and Cochrane Library electronic databases. STUDY SELECTION: Studies with an exercise-based intervention published by December 31, 2021 were identified. RCTs and meta-analyses involving adults with depression were also included; 10 studies were selected, including a total of 956 subjects. STUDY DESIGN: Systematic review and meta-analysis. LEVEL OF EVIDENCE: Level 1. RESULTS: Effect sizes were summarized using standardized mean differences (95% confidence interval) by effected randomized models. The results reinforce that exercise appears to be beneficial in improving depression among adults aged 18 to 65 years. Interventions lasting above 150 minutes per week of moderate intensity and group interventions seem to have a more significant effect on reducing depression. Studies have revealed that aerobic exercise, compared with resistance or flexibility, has a more positive effect on depression. CONCLUSION: PE can be a way to reduce depression and can be used as a possible adjunctive tool for pharmacological and/or alternative treatments. Considering the findings of this study, it is important that health professionals (eg, exercise physiologists, physicians, nurses, psychologists) promote the practice of PE as a complementary alternative and act early to prevent the worsening of depression. PROSPERO REGISTRATION NUMBER: CRD42020188909.

Testing Assumptions of the Physical Activity Adoption and Maintenance Model: A Longitudinal Perspective of the Relationships Between Intentions and Habits on Exercise Adherence.

In this study, we aimed to examine empirically the Physical Activity Adoption and Maintenance model (PAAM). We collected data on these variables at baseline (T0) and 6-months later (T1). We recruited 119 participants (42 male, 77 female) aged 18-81 years old (Mage = 44.89, SD = 12.95). who reported, at baseline, that they exercised an average of 3.76 days per week (SD = 1.33) in training periods lasting 15-60 minutes (M = 38.69; SD = 23.28). We conducted hierarchical multiple regression analysis to test the association between each determinant (intentions, habits, and frequency) and future exercise adherence. We tested four models by entering blocks of predictors according to PAAM assumptions. The variance change (R2) between the first and fourth models (ΔR2 = .391) was statistically significant, showing that the fourth model accounted for 51.2% of variance for future exercise adherence, F (6, 112) = 21.631, p < .001, R2 = .73, adjusted R2 = .512. Exercise intention at T1 maintained its significant association (p = .021) with exercise frequency at T1 in all tested models. Exercise frequency at T0 was the most significant predictor (p < .01) of future exercise adherence, with past experience the second most significant predictor (p = .013). Interestingly, exercise habits at T1 and T0 did not predict exercise frequency at T1 in the fourth model. Among the variables we studied, having constantly high exercise intentions and high regular exercise frequency are significantly associated with maintaining or increasing regular future exercise behavior.

Assessing Affective Valence and Activation in Stretching Activities with the Feeling Scale and the Felt Arousal Scale: A Systematic Review.

Affective responses have been considered key determinants for exercise adherence, but research on affective responses to stretching activities is scarce. Given the role of these responses in exercise adherence, our aim in this review was to explore (a) the utility and feasibility of core affect in stretching-related activities as measured by the Feeling Scale (FS) and/or the Felt Arousal Scale (FAS); (b) the timing of administering these scales; and (c) the scales' applicability and interpretability in this context. Inclusion criteria for studies in this review were experimental and non-experimental studies written in English that based affect assessment on the FS and/or FAS and that applied these scales to participants engaged in physical activity, individually or in groups. We also considered studies that focused on stretching activities that were either isolated or components of a class/activity and studies that used healthy participants of any age. Exclusion criteria were populations with mental health problems, cancer, diabetes, hypertension, cardiovascular disease, or diseases likely to alter pain perception or be associated with chronic pain, instrument validation studies, gray literature, and systematic reviews. We searched PubMed, SPORTDiscus and PsycINFO databases, and we added studies retrieved manually from reference sections while following PRISMA guidelines. We used the Effective Public Health Practice Project tool for judging methodological quality of research articles. Our final analyses were based on 12 empirical studies published between 2003 and 2021with a total of 718 participants. Both scales were found to be useful and feasible in the most usual places for exercise, but core affect results cannot be properly interpreted due to variability of study protocols and the absence of guidelines for adequate baseline assessment. Most studies recorded affect responses pre-session, during session, and post-session. We observed no standardized timing or frequency of assessment, and there was high heterogeneity among stretching protocols. Currently, research in core affect assessment of stretching-related activities lacks sufficient methodological quality to draw generalizable conclusions.

Using passive Wi-Fi for community crowd sensing during the COVID-19 pandemic.

Sensing passersby and detecting crowded locations is a growing area of research and development in the last decades. The COVID-19 pandemic compelled authorities and public and private institutions to monitor access and occupancy of crowded spaces. This work addresses the detection of crowds in points of interest (POI) by using a territory grid analysis categorizing POIs by the services available in each location and comparing data gathered from a community passive Wi-Fi infrastructure against mobile cellular tower association data from telecom companies. In Madeira islands (Portugal), we used data from the telecom provider NOS for the timespan of 4 months as ground truth and found a strong correlation with sparse passive Wi-Fi. An official regional mobile application shows the occupancy data to end-users based on the territory categorization and the passive Wi-Fi infrastructure in POIs. Occupancy data shows historical hourly trends of each location, and the real-time occupation, helping visitors and locals plan their commutes better to avoid crowded spaces.

Assessing affective valence and activation in resistance training with the feeling scale and the felt arousal scale: A systematic review.

Evidence suggests affective responses to exercise can influence exercise adherence. However, there is a limited understanding of how and when to measure core affect in resistance training. As such, the objective of this systematic review was to analyze how the Feeling Scale and/or the Felt Arousal Scale have been used in resistance training to assess core affect. Focus was given to the contextual feasibility, timing, and frequency of assessment. A search in PubMed, SPORTDiscus, and PsycINFO databases was conducted (last search date July, 2022) with the purpose of including experimental and non-experimental studies, utilizing the Feeling Scale and/or the Felt Arousal Scale in resistance training, and focused on apparently healthy individuals of any age. Twenty-seven studies (N = 718 participants) published between 2009-2022 were qualitatively analyzed. Both scales appeared to be able to detect core affect within a wide array of intensities, ages, and equipment. As for the timing and frequency of measurement, no apparent standardization was evident. The use of the Feeling Scale, the Felt Arousal Scale, or both, to measure core affect appears to be feasible in resistance training practices. However, a lack of methodological background raises concerns regarding the quality of previous studies' assessments and comparisons of results across studies.

Exploring the impact of individualized pleasure-oriented exercise sessions in a health club setting: Protocol for a randomized controlled trial.

Research on the relationship between exercise-induced affect and exercise or physical activity behavior has gained momentum in recent years, yielding several observational and longitudinal studies. However, experimental tests demonstrating a causal role of affective responses on exercise adherence are lacking. Given the need to devise exercise prescriptions that can facilitate adherence and promote regular physical activity, a Randomized Controlled Trial targeting individual pleasurable responses in a health-club setting will be conducted. The experimental protocol will compare two individualized evaluation, prescription, and supervision methods, adjusted for intensity, with the aim to explore their impact on behavioral, affective, and motivational outcomes. The planned study will be a pragmatic randomized, single-blinded, controlled superiority trial with two parallel groups and an allocation ratio of 1:1. Apparently healthy volunteer participants (N = 46) between 18 and 45 years old, who are not regularly active, will be randomly allocated to a control or experimental group. The control group will follow a general American College of Sports Medicine exercise prescription; the experimental group will follow the same prescription but with enhancements aimed at promoting pleasure: (1) individualization based on individual differences in preference for and tolerance of exercise intensity; (2) self-regulation of intensity; and (3) repeated assessments of core affect. The primary outcome will be post-intervention exercise-session attendance. Affective and motivational variables will be examined as secondary outcomes. The results are expected to advance exercise prescriptions by promoting affective regulation, thus helping to improve exercise adherence and related outcomes. TRIAL REGISTRATION: ClinicalTrials.gov; ID: NCT05416593.

Affective responses to stretching exercises: Exploring the timing of assessments.

Affective responses during exercise have been identified as a predictor of exercise adherence. However, research has been mostly limited to aerobic and resistance exercise. Considering that stretching activities are also an important component of physical fitness, this quasi-experimental study was designed to: 1) compare affective responses during and immediately after stretching exercises in apparently healthy adults, and 2) assess the consistency and repeatability of affect ratings obtained one week apart. For this purpose, we analyzed the Feeling Scale (FS) and Felt Arousal Scale (FAS) ratings using Time (during and after stretching) x Intensity (light, moderate, vigorous) x Stretched Muscle Group (quadriceps, hamstrings, glutes, latissimus dorsi, triceps) with repeated measures analysis of variance (ANCOVA) in 34 participants (21 males; aged 32.8 ± 8.6 years). The repeatability of FS and FAS ratings was assessed using two-way random-effects models, Intraclass Correlation Coefficients (ICC), and Bland-Altman plots. FS scores were higher following the stretching exercises, whereas FAS scores were lower, particularly in the vigorous intensity. In general, the inter-day repeatability for FS and FAS measurements was good across muscle groups. ICC tended to be higher at vigorous intensities. Ratings of core affect can be collected during static passive stretches using the FAS and FAS in ecologically valid settings. These results suggest that an adequate assessment of core affective responses to stretching activities should be performed during the exercises.