RESUMO
The basal ganglia are a highly interconnected network of nuclei essential for the modulation and execution of voluntary behavior. The neostriatum is the principal input and one of the principal controllers of the output of the basal ganglia. Neostriatal projection neurons seem to be dynamically and powerfully controlled by GABAergic inputs, but the source(s) and physiological properties of these inputs remain unclear. Here we use paired whole-cell recordings to show that this inhibition derives from small populations of GABAergic interneurons that are themselves interconnected through functional electrotonic synapses. Inhibitory synaptic potentials generated from single interneurons are sufficiently powerful to delay or entirely block the generation of action potentials in a large number of projection neurons simultaneously.
Assuntos
Interneurônios/fisiologia , Neostriado/fisiologia , Inibição Neural , Neurônios/fisiologia , Ácido gama-Aminobutírico/fisiologia , Potenciais de Ação/fisiologia , Animais , Vias Neurais/fisiologia , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-DawleyRESUMO
This is the introductory chapter to an edited volume comprising 18 chapters written by 38 specially selected authors covering the anatomy, physiology, biochemistry/pharmacology and behavioral aspects of GABA in the basal ganglia. In this chapter the various nuclei of the basal ganglia are defined and their cellular structure, connections and function reviewed in brief in order to provide an orientation for the subsequent 17 chapters.
Assuntos
Gânglios da Base/anatomia & histologia , Gânglios da Base/fisiologia , Vias Neurais/anatomia & histologia , Vias Neurais/fisiologia , Neurotransmissores/fisiologia , Animais , Corpo Estriado/anatomia & histologia , Corpo Estriado/fisiologia , Humanos , Inibição Neural/fisiologia , Substância Negra/anatomia & histologia , Substância Negra/fisiologia , Núcleo Subtalâmico/anatomia & histologia , Núcleo Subtalâmico/fisiologia , Transmissão Sináptica/fisiologia , Ácido gama-Aminobutírico/fisiologiaRESUMO
Spontaneous firing rates and changes in firing rate in response to an intravenously administered dose of apomorphine were measured after various electroconvulsive shock (ECS) treatment regimens from dopaminergic cells of the substantia nigra in urethane-anesthetized rats. Similar measurements were obtained from noradrenergic neurons of the locus coeruleus before and after intravenous injection of clonidine. A significant decrement in the inhibition of spontaneous firing in response to intravenous administration of these agonists was observed following multiple or single ECS treatment in both substantia nigra and locus coeruleus cells. There was a consistent but nonsignificant tendency for cells in both areas of the brain from treated animals to display higher rates of spontaneous firing than their respective sham-shocked controls. Both the effects on base-line rates of spontaneous activity and on the depression of firing rate in response to drug administration were found to be independent of repeated treatment. A significant negative correlation was obtained between base-line firing rate and percentage depression to the autoreceptor agonist, but this correlation alone was insufficient to account for the observed differences in the drug response. These results are discussed with respect to possible mechanisms of action of electroconvulsive therapy in the treatment of depression.
Assuntos
Catecolaminas/metabolismo , Eletrochoque , Locus Cerúleo/efeitos dos fármacos , Receptores Colinérgicos/efeitos dos fármacos , Substância Negra/efeitos dos fármacos , Transmissão Sináptica/efeitos dos fármacos , Animais , Apomorfina/farmacologia , Clonidina/farmacologia , Relação Dose-Resposta a Droga , Potenciais Evocados/efeitos dos fármacos , Masculino , Neurônios/efeitos dos fármacos , Ratos , Ratos Endogâmicos , Receptores Adrenérgicos/efeitos dos fármacos , Receptores Dopaminérgicos/efeitos dos fármacosRESUMO
Cholinergic neurons in the basal forebrain are the focus of considerable interest because they are severely affected in Alzheimer's disease. However, both cholinergic and noncholinergic neurons are intermingled in this region. The goal of the present study was to characterize the morphology and in vivo electrophysiology of noncholinergic basal forebrain neurons. Neurons in the ventral pallidum and substantia innominata were recorded extracellularly, labeled juxtacellularly with biocytin and characterized for the presence of choline acetyltransferase immunoreactivity. Two types of ventral pallidal cells were observed. Type I ventral pallidal neurons had axons that rarely branched near the cell body and tended to have smaller somata and lower spontaneous firing rates than did type II ventral pallidal neurons, which displayed extensive local axonal arborizations. Subtypes of substantia innominata neurons could not be distinguished based on axonal morphology. These noncholineregic neurons exhibited local axon arborizations along a continuum that varied from no local collaterals to quite extensive arbors. Substantia innominata neurons had lower spontaneous firing rates, more variable interspike intervals, and different spontaneous firing patterns than did type II ventral pallidal neurons and could be antidromically activated from cortex or substantia nigra, indicating that they were projection neurons. Ventral pallidal neurons resemble, both morphologically and electrophysiologically, previously described neurons in the globus pallidus, whereas the substantia innominata neurons bore similarities to isodendritic neurons of the reticular formation. These results demonstrate the heterogeneous nature of noncholinergic neurons in the basal forebrain.
Assuntos
Mapeamento Encefálico/métodos , Globo Pálido/fisiologia , Neurônios/fisiologia , Substância Inominada/fisiologia , Acetilcolina , Animais , Globo Pálido/citologia , Processamento de Imagem Assistida por Computador , Masculino , Ratos , Ratos Sprague-Dawley , Substância Inominada/citologiaRESUMO
The connectivity of a region surrounding the established thalamic auditory nuclei, n. ovoidalis (Ov) and n. semilunaris parovoidalis (SPO), was explored in the ring dove by using the anterograde tracers, Phaseolus vulgaris leucoagglutinin (PHAL) and biocytin, and the retrograde tracer, fluorogold. The Ov-SPO surround received a projection from a cell group along the interface of the auditory midbrain and the n. intercollicularis, as revealed with PHAL and biocytin, and was composed of neurons exhibiting a common morphology. These features and the presence of overlapping projections from different portions of the Ov-SPO surround suggest that this region comprises a functionally discrete area, which we term the Ov shell. Single unit recording within the shell established the existence of acoustically responsive units. Both PHAL and fluorogold labeling revealed a robust projection from the Ov shell to the caudomedial hypothalamus. Major telencephalic projections of the shell terminated within the ventral paleostriatal complex, "end-zones" of the field L, the caudomedial hyperstriatum ventrale, and regions immediately dorsal and lateral to the auditory neostriatum. Except for a portion of the shell bordering medial ovoidalis, PHAL injections into the shell also labeled fibers within the caudolateral neostriatum and along the lateral neostriatal rim. The connectivity of the Ov shell suggests that this region may integrate auditory pathways with brain regions associated with endocrine mediated behavior. In addition, the shell may constitute a source of converging input to several levels of central auditory pathways.
Assuntos
Columbidae/fisiologia , Audição/fisiologia , Estilbamidinas , Núcleos Talâmicos/anatomia & histologia , Estimulação Acústica , Animais , Vias Auditivas/anatomia & histologia , Eletrofisiologia , Feminino , Corantes Fluorescentes , Histocitoquímica , Hipotálamo/anatomia & histologia , Hipotálamo/fisiologia , Lisina/análogos & derivados , Masculino , Fito-HemaglutininasRESUMO
The distribution and density of asymmetric synapses including biocytin-labelled corticostriatal synapses of the rat neostriatum were examined at postnatal day 10 (P10), P15, P21 and in adults. The density of asymmetric synapses in the adult neostriatum (28.0 synapses/100 microm2) was significantly greater than that in neonates at P15 (14.4 synapses/100 microm2) and P10 (11.5 synapses/100 microm2), but not at P21 (24.2+/-1.5 synapses/100 microm2). The increased density of asymmetric synapses in the adult neostriatum was due primarily to an increase in the number of axospinous synapses. The density of axospinous synapses was greatest in adults (22.3 synapses/100 microm2) and significantly less at P21 (15.3 synapses/100 microm2), P15 (5.9 synapses/100 microm2), and P10 (2.0 synapses/100 microm2). The density of axodendritic synapses, however, remained similar at all ages (adult, 3.9+/-1.1 synapses/100 microm2; P21, 6.0+/-1.2 synapses/100 microm2; P15, 5.7+/-0.8 synapses/100 microm2 or P10, 7.2+/-1.3 synapses/100 microm2). Iontophoretic injection of biocytin into the lateral frontal agranular cortex produced labelling of corticostriatal afferents which formed asymmetric synapses in the neostriatum. The distribution of termination sites of biocytin-labelled corticostriatal boutons showed a pattern of development similar to the unlabelled asymmetric synapses. The present study shows that the increase in the overall number of asymmetric synapses over the first three postnatal weeks can be attributed to an increase in the density of asymmetric axospinous synapses. During the same period little change is noted in the number or density of asymmetric axodendritic synapses. These changes in excitatory synaptic input to medium spiny neurons may explain some of the previously described electrophysiological differences noted between the neonatal and adult neostriatum.
Assuntos
Neostriado/crescimento & desenvolvimento , Neostriado/ultraestrutura , Sinapses/fisiologia , Sinapses/ultraestrutura , Envelhecimento/fisiologia , Animais , Axônios/fisiologia , Axônios/ultraestrutura , Imuno-Histoquímica , Lisina/análogos & derivados , Lisina/metabolismo , Microscopia Eletrônica , Neostriado/fisiologia , Fibras Nervosas/fisiologia , Fibras Nervosas/ultraestrutura , Neurônios Aferentes/fisiologia , Neurônios Aferentes/ultraestrutura , Ratos , Ratos Sprague-DawleyRESUMO
A subpopulation of inactive or "silent" dopaminergic neurons has been reported to exist in vivo in rat substantia nigra, comprising up to 50% of nigral dopaminergic neurons. The existence of this large proportion of silent neurons has been inferred from various experimental manipulations, but never demonstrated directly. In the present study, striatal or medial forebrain bundle stimulation was used to activate antidromically substantia nigra dopaminergic neurons in vivo. Antidromic spikes of dopaminergic neurons observed by extracellular single-unit recordings in the absence of spontaneous activity were employed as indicators of the presence of a silent cell. A total of 312 dopamine neurons were recorded, including 190 neurons that could be antidromically activated from the striatum and/or the medial forebrain bundle. All neurons exhibited spontaneous activity. The firing rates were unimodally distributed about the mean of 4 spikes/s, and very few cells were observed to fire at less than 0.5 spikes/s. The numbers of spontaneously active and antidromically activated dopaminergic neurons per track were recorded and compared with the number of antidromically responding silent dopaminergic neurons per track after systemic apomorphine administration. Under control conditions, 0.80 +/- 0.10 or 1.36 +/- 0.13 spontaneously active neurons per track could be antidromically activated at 1.0 mA by striatal or medial forebrain bundle stimulation, respectively. After apomorphine completely suppressed spontaneous activity, 0.69 +/- 0.08 and 1.39 +/- 0.14 antidromic neurons per track were detected by stimulating the striatum or medial forebrain bundle respectively at 1.0 mA, demonstrating that silent dopaminergic neurons can be reliably identified through antidromic activation. In sharp contrast to previous reports, these data suggest that silent neurons do not comprise a substantial proportion of the total number of dopaminergic neurons in the substantia nigra. Reverse chi2 analysis revealed that, if they exist at all, silent dopaminergic neurons make up less than 2% of the dopaminergic cells in the substantia nigra. These findings are related to current theories of the mechanisms of action of antipsychotic drugs and the maintenance of near-normal levels of dopamine in the striatum following large-scale loss of nigral dopaminergic neurons.
Assuntos
Dopamina/fisiologia , Neurônios/fisiologia , Substância Negra/citologia , Substância Negra/fisiologia , Animais , Apomorfina/farmacologia , Contagem de Células , Agonistas de Dopamina/farmacologia , Estimulação Elétrica , Eletrofisiologia , Masculino , Potenciais da Membrana/efeitos dos fármacos , Potenciais da Membrana/fisiologia , Neurônios/efeitos dos fármacos , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-Dawley , Substância Negra/efeitos dos fármacosRESUMO
Significant changes in the neurophysiology and neuropharmacology of nigral dopaminergic neurons take place in the first postnatal month. In order to correlate these changes with the postnatal development of dopaminergic neuron morphology and substantia nigra cytoarchitecture, brains from Sprague-Dawley rat pups of age postnatal days 1, 7, 14, 21 and 28 and adult rats were sectioned and processed for tyrosine hydroxylase immunocytochemistry. At postnatal day 1, pars compacta and pars reticulata were not clearly delineated; tyrosine hydroxylase positive neurons and a dense plexus of fibers were scattered throughout the substantia nigra. By day 7 the density of tyrosine hydroxylase positive neurons decreased markedly in ventral substantia nigra, and a dopaminergic pars compacta and a non-dopaminergic pars reticulata could be more clearly distinguished. By day 14 the substantia nigra appeared essentially as it does in the adult. Cell counts during development revealed that the number of tyrosine hydroxylase positive neurons/section in both pars compacta and pars reticulata decreased significantly from postnatal day 1 to postnatal day 14, while those in pars lateralis did not change. Tyrosine hydroxylase-positive somatic size increased modestly but significantly from postnatal day 1 to day 14 as did the diameter of the proximal and distal dendrites. However, even at day 1, the morphology of tyrosine hydroxylase positive neurons appeared essentially the same as in adults. Dendritic arborizations were well developed. The dendrites were non-varicose and modestly branched, with some of the longer ventrally directed dendrites passing through pars reticulata into the crus cerebri.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Envelhecimento/fisiologia , Neurônios/fisiologia , Substância Negra/fisiologia , Tirosina 3-Mono-Oxigenase/metabolismo , Animais , Animais Recém-Nascidos , Feminino , Imuno-Histoquímica , Masculino , Fibras Nervosas/enzimologia , Fibras Nervosas/ultraestrutura , Neurônios/citologia , Neurônios/enzimologia , Especificidade de Órgãos , Ratos , Ratos Sprague-Dawley , Substância Negra/citologia , Substância Negra/crescimento & desenvolvimento , Tirosina 3-Mono-Oxigenase/análiseRESUMO
Dopaminergic neurons express both GABA(A) and GABA(B) receptors and GABAergic inputs play a significant role in the afferent modulation of these neurons. Electrical stimulation of GABAergic pathways originating in neostriatum, globus pallidus or substantia nigra pars reticulata produces inhibition of dopaminergic neurons in vivo. Despite a number of prior studies, the identity of the GABAergic receptor subtype(s) mediating the inhibition evoked by electrical stimulation of neostriatum, globus pallidus, or the axon collaterals of the projection neurons from substantia nigra pars reticulata in vivo remain uncertain. Single-unit extracellular recordings were obtained from substantia nigra dopaminergic neurons in urethane anesthetized rats. The effects of local pressure application of the selective GABA(A) antagonists, bicuculline and picrotoxin, and the GABA(B) antagonists, saclofen and CGP-55845A, on the inhibition of dopaminergic neurons elicited by single-pulse electrical stimulation of striatum, globus pallidus, and the thalamic axon terminals of the substantia nigra pars reticulata projection neurons were recorded in vivo. Striatal, pallidal, and thalamic induced inhibition of dopaminergic neurons was always attenuated or completely abolished by local application of the GABA(A) antagonists. In contrast, the GABA(B) antagonists, saclofen or CGP-55845A, did not block or attenuate the stimulus-induced inhibition and at times even increased the magnitude and/or duration of the evoked inhibition. Train stimulation of globus pallidus and striatum also produced an inhibition of firing in dopaminergic neurons of longer duration. However this inhibition was largely insensitive to either GABA(A) or GABA(B) antagonists although the GABA(A) antagonists consistently blocked the early portion of the inhibitory period indicating the presence of a GABA(A) component. These data demonstrate that dopaminergic neurons of the substantia nigra pars compacta are inhibited by electrical stimulation of striatum, globus pallidus, and the projection neurons of substantia nigra pars reticulata in vivo. This inhibition appears to be mediated via the GABA(A) receptor subtype, and all three GABAergic afferents studied appear to possess inhibitory presynaptic GABA(B) autoreceptors that are active under physiological conditions in vivo.
Assuntos
Corpo Estriado/fisiologia , Dopamina/fisiologia , Antagonistas GABAérgicos/farmacologia , Globo Pálido/fisiologia , Neurônios/efeitos dos fármacos , Receptores de GABA-A/fisiologia , Substância Negra/fisiologia , Ácido gama-Aminobutírico/fisiologia , Vias Aferentes/efeitos dos fármacos , Vias Aferentes/fisiologia , Animais , Baclofeno/análogos & derivados , Baclofeno/farmacologia , Bicuculina/farmacologia , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/lesões , Estimulação Elétrica , Antagonistas de Receptores de GABA-A , Globo Pálido/efeitos dos fármacos , Masculino , Degeneração Neural , Neurônios/fisiologia , Ácidos Fosfínicos/farmacologia , Picrotoxina/farmacologia , Propanolaminas/farmacologia , Ratos , Ratos Sprague-Dawley , Receptores de GABA-B/efeitos dos fármacos , Receptores Pré-Sinápticos/efeitos dos fármacos , Receptores Pré-Sinápticos/fisiologia , Substância Negra/efeitos dos fármacos , Substância Negra/lesõesRESUMO
Dopaminergic neurons in vivo fire spontaneously in three distinct patterns or modes. It has previously been shown that the firing pattern of substantia nigra dopaminergic neurons can be differentially modulated by local application of GABA(A) and GABA(B) receptor antagonists. The GABA(A) antagonists, bicuculline or picrotoxin, greatly increase burst firing in dopaminergic neurons whereas GABA(B) antagonists cause a modest shift away from burst firing towards pacemaker-like firing. The three principal GABAergic inputs to nigral dopaminergic neurons arise from striatum, globus pallidus and from the axon collaterals of nigral pars reticulata projection neurons, each of which appear to act in vivo primarily on GABA(A) receptors (see preceding paper). In this study we attempted to determine on which afferent pathway(s) GABA(A) antagonists were acting to cause burst firing. Substantia nigra dopaminergic neurons were studied by single unit extracellular recordings in urethane anesthetized rats during pharmacologically induced inhibition and excitation of globus pallidus. Muscimol-induced inhibition of pallidal neurons produced an increase in the regularity of firing of nigral dopaminergic neurons together with a slight decrease in firing rate. Bicuculline-induced excitation of globus pallidus neurons produced a marked increase in burst firing together with a modest increase in firing rate. These changes in firing rate were in the opposite direction to what would be expected for a monosynaptic GABAergic pallidonigral input. Examination of the response of pars reticulata GABAergic neurons to similar manipulations of globus pallidus revealed that the firing rates of these neurons were much more sensitive to changes in globus pallidus neuron firing rate than dopaminergic neurons and that they responded in the opposite direction. Pallidal inhibition produced a dramatic increase in the firing rate of pars reticulata GABAergic neurons while pallidal excitation suppressed the spontaneous activity of pars reticulata GABAergic neurons. These data suggest that globus pallidus exerts significant control over the firing rate and pattern of substantia nigra dopaminergic neurons through a disynaptic pathway involving nigral pars reticulata GABAergic neurons and that at least one important way in which local application of bicuculline induces burst firing of dopaminergic neurons is by disinhibition of this tonic inhibitory input.
Assuntos
Dopamina/fisiologia , Agonistas GABAérgicos/farmacologia , Antagonistas GABAérgicos/farmacologia , Globo Pálido/fisiologia , Neurônios/fisiologia , Substância Negra/fisiologia , Ácido gama-Aminobutírico/fisiologia , Potenciais de Ação/efeitos dos fármacos , Vias Aferentes/efeitos dos fármacos , Vias Aferentes/fisiologia , Animais , Bicuculina/farmacologia , Relógios Biológicos/fisiologia , Estimulação Elétrica , Globo Pálido/efeitos dos fármacos , Masculino , Muscimol/farmacologia , Neurônios/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Substância Negra/efeitos dos fármacosRESUMO
The morphology and synaptic organization of neurons in the ventroanterior-ventrolateral nucleus of rats was examined using in vivo intracellular staining techniques. Neurons were characterized electrophysiologically based on intrinsic membrane properties and synaptic responses to stimulation of motor cortex and cerebellar nuclei, as described in the companion paper. Cerebellar-responsive neurons were stained intracellularly with either horseradish peroxidase or biocytin. All stained ventroanterior-ventrolateral nucleus neurons were identified as thalamocortical neurons on anatomical (and often electrophysiological) grounds, consistent with previous findings that rat ventroanterior-ventrolateral nucleus is interneuron-sparse. Ventroanterior-ventrolateral nucleus neurons had three to eight thick primary dendrites. Proximal dendrites often exhibited a tufted branching pattern, from which many thinner, higher order dendrites arose. Dendrites branched to form a funnel-like infiltration of the neuropil that resulted in a spherical, roughly homogeneous dendritic field. The axon originated from the cell body or a proximal dendrite and coursed laterally and dorsally to innervate motor cortex. One to five axon collaterals were emitted in the rostral dorsolateral sector of the thalamic reticular nucleus; collaterals were not observed in the ventroanterior-ventrolateral nucleus or other nuclei in dorsal thalamus. The synaptic organization of the ventroanterior-ventrolateral nucleus was examined with electron microscopy, including two intracellularly labeled ventroanterior-ventrolateral nucleus neurons that were shown electrophysiologically to receive monosynaptic inputs from the cerebellum. The neuropil of rat ventroanterior-ventrolateral nucleus lacked the complexity and diversity found in corresponding thalamic nuclei of felines and primates, due to the paucity of interneurons. Vesicle-containing dendrites, dendrodendritic synapses and glomeruli were not observed. Three broad classes of presynaptic terminals were identified. (1) Small round boutons: small boutons containing densely-packed, small round vesicles that formed asymmetric synapses predominantly with the distal dendrites of thalamocortical neurons. These were the most prevalent type of bouton in the ventroanterior-ventrolateral nucleus (78% of presynaptic elements) and likely arose from the cerebral cortex. (2) Large round boutons: large terminals with loosely packed small round vesicles that made multiple asymmetric synapses with proximal and intermediate dendrites. Large round boutons comprised 8% of the neuropil, and likely arose from the cerebellar nuclei. (3) Medium size boutons with pleomorphic vesicles: medium-sized profiles containing pleomorphic vesicles that formed symmetric synapses with proximal, intermediate and distal dendrites and, less frequently, with cell bodies.(ABSTRACT TRUNCATED AT 400 WORDS)
Assuntos
Cerebelo/fisiologia , Neurônios/fisiologia , Núcleos Talâmicos/fisiologia , Animais , Axônios/ultraestrutura , Cerebelo/citologia , Cerebelo/ultraestrutura , Dendritos/ultraestrutura , Estimulação Elétrica , Eletrofisiologia , Histocitoquímica , Peroxidase do Rábano Silvestre , Lisina/análogos & derivados , Masculino , Microscopia Eletrônica , Córtex Motor/citologia , Córtex Motor/fisiologia , Neurônios/ultraestrutura , Ratos , Ratos Sprague-Dawley , Sinapses/fisiologia , Sinapses/ultraestrutura , Núcleos Talâmicos/citologia , Núcleos Talâmicos/ultraestruturaRESUMO
The firing patterns of dopaminergic neurons in vivo are strongly modulated by afferent input. The principal GABAergic inputs to the dopaminergic neurons of the substantia nigra originate from neurons of the neostriatum, globus pallidus and substantia nigra pars reticulata. It has previously been shown that the firing pattern of nigral dopaminergic neurons can be manipulated by pharmacologically induced excitation or inhibition of the globus pallidus with relatively little effect on firing rate. We used this technique to explore the relation between the firing pattern of dopaminergic neurons and extracellular dopamine levels in the neostriatum in vivo. Specifically, we tested whether an increase in burst firing in dopaminergic neurons produced by increased pallidal activity led to increased extracellular dopamine levels in the neostriatum. Single unit extracellular recording combined with simultaneous microdialysis was used to measure the firing rates and patterns of dopaminergic neurons and extracellular striatal dopamine levels, respectively, during bicuculline-induced excitation of the globus pallidus. Pallidal excitation resulted in a marked increase in burst firing in dopaminergic neurons along with only a slight increase in firing rate, but produced a significant elevation (approximately 45%) in neostriatal dopamine levels. These data suggest that afferent-induced burst firing in dopaminergic neurons leads to an increase in extracellular dopamine levels in the neostriatum when compared with less bursty patterns with similar overall firing rates.
Assuntos
Dopamina/fisiologia , Espaço Extracelular/fisiologia , Globo Pálido/fisiologia , Neostriado/fisiologia , Neurônios/fisiologia , Substância Negra/fisiologia , Animais , Bicuculina/farmacologia , Dopamina/metabolismo , Eletrofisiologia , Espaço Extracelular/efeitos dos fármacos , Espaço Extracelular/metabolismo , Antagonistas GABAérgicos/farmacologia , Globo Pálido/citologia , Globo Pálido/efeitos dos fármacos , Masculino , Microdiálise , Neostriado/efeitos dos fármacos , Neostriado/metabolismo , Neurônios/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Substância Negra/citologia , Substância Negra/efeitos dos fármacos , Sinapses/efeitos dos fármacos , Sinapses/fisiologiaRESUMO
The effects of amphetamine upon the terminal excitability of noradrenergic neurons of the nucleus locus coeruleus were studied in urethane anesthetized rats. Terminal excitability was measured by determining the stimulus currents necessary to evoke antidromic responses in locus coeruleus neurons from terminals in the frontal cortex. In most cases, terminal excitability was decreased following local infusion of amphetamine into the frontal cortex, while intravenous administration of the drug tended to increase terminal excitability. The decreased terminal excitability induced by local infusion of amphetamine appeared to be due to activation of alpha-adrenergic receptors located on the terminals of locus coeruleus neurons, since this effect mimics that of clonidine, a direct acting alpha-adrenergic agonist, and since the effect was abolished by pretreatment with alpha-methyl-p-tyrosine which disrupts the catecholamine liberating properties of amphetamine. Phentolamine, a direct acting alpha-adrenergic receptor antagonist was also found to block or reverse the effect of amphetamine. The changes in terminal excitability following intravenous injection of amphetamine appeared to be related to changes in the spontaneous activity of locus coeruleus neurons. A large decrease in spontaneous activity following intravenous administration of amphetamine was associated with increased terminal excitability, whereas when smaller changes in spontaneous activity occurred, terminal excitability was found to be decreased. These results are discussed with respect to the pharmacological properties of catecholaminergic neurons and the mechanisms of action of amphetamine.
Assuntos
Dextroanfetamina/farmacologia , Lobo Frontal/efeitos dos fármacos , Locus Cerúleo/efeitos dos fármacos , Norepinefrina/fisiologia , Receptores Adrenérgicos/efeitos dos fármacos , Transmissão Sináptica/efeitos dos fármacos , Animais , Relação Dose-Resposta a Droga , Masculino , Neurônios/efeitos dos fármacos , Ratos , Ratos Endogâmicos , Tempo de Reação/efeitos dos fármacosRESUMO
In vivo intracellular recordings were obtained from identified thalamocortical neurons in the ventroanterior-ventrolateral complex in urethane-anesthetized rats. This thalamic nucleus has few interneurons. Neurons that responded to cerebellar stimulation were injected intracellularly with horseradish peroxidase or biocytin and examined with light and electron microscopy (see companion paper). Intrinsic membrane properties and voltage-dependent rhythmic activity of cerebellar-responsive ventroanterior-ventrolateral neurons were similar to those described previously for thalamic neurons. Thus, in addition to conventional "fast" Na(+)-dependent spikes, rat ventroanterior-ventrolateral neurons had "slow" Ca(2+)-mediated low-threshold spikes and membrane conductances that supported rhythmic oscillations. Two modes of spontaneous activity were observed: (i) a tonic firing pattern that consisted of irregularly occurring fast spikes that predominated when the membrane potential was more positive than about -60 mV, and (ii) a rhythmic firing pattern, observed when the membrane potential was more negative than about -65 mV, composed of periodic (4-8 Hz) membrane hyperpolarizations and ramp depolarizations that often produced a low-threshold spike and a burst of fast spikes. In some neurons, spontaneous fast prepotentials were also observed, often with a relatively constant rate (up to 70 Hz). Cerebellar stimulation elicited excitatory postsynaptic potentials that in some cases appeared to be all-or-none and were similar in form to fast prepotentials. Stimulation of ipsilateral motor cortex elicited a short-latency antidromic response followed by a monosynaptic excitatory postsynaptic potential, which had a slower rise time than excitatory postsynaptic potentials evoked from cerebellum, suggesting that cortical inputs were electrotonically distal to cerebellar inputs. In the presence of moderate membrane hyperpolarization, the cortically evoked excitatory postsynaptic potential was followed by a long-lasting hyperpolarization (100-400 ms duration), a rebound depolarization and one or two cycles resembling spontaneous rhythmic activity. Membrane conductance was increased during the initial component of the long hyperpolarization, much of which was probably due to an inhibitory postsynaptic potential. In contrast, membrane conductance was unchanged or slightly decreased during the latter three-quarters of the long hyperpolarization. The amplitude of this component of the long hyperpolarization usually decreased when the membrane was hyperpolarized with intracellular current injection. Thus, both disfacilitation and an inhibitory postsynaptic potential may have contributed to the latter portion of the cortically-evoked long hyperpolarization. The cortically-evoked inhibitory postsynaptic potentials likely originated predominantly from feedforward activation of GABAergic neurons in the thalamic reticular nuclei.(ABSTRACT TRUNCATED AT 400 WORDS)
Assuntos
Cerebelo/fisiologia , Neurônios/fisiologia , Núcleos Talâmicos/fisiologia , Animais , Cerebelo/citologia , Córtex Cerebral/citologia , Córtex Cerebral/fisiologia , Estimulação Elétrica , Eletrofisiologia , Interneurônios/fisiologia , Masculino , Potenciais da Membrana/fisiologia , Córtex Motor/fisiologia , Neurônios/ultraestrutura , Ratos , Ratos Sprague-Dawley , Sinapses/fisiologia , Núcleos Talâmicos/citologia , Ácido gama-Aminobutírico/fisiologiaRESUMO
Both spontaneous and evoked extracellular electrophysiological activity of neurons within fetal mesencephalon suspension grafts to the dopamine-depleted striatum of rats were examined. In some cases, extracellular recording was combined with intracellular labeling to identify recorded neurons. Grafted rats displaying a complete cessation of ipsilateral rotations following amphetamine administration were examined at post-implantation time intervals of two, four, five, eight and nine months. Four separate classes of neurons were distinguished within the transplanted striatum based on electrophysiological properties. The first of these groups, the type I cells, appeared to be non-grafted striatal neurons. When spontaneously active, these striatal-like cells fired bursts of action potentials separated by periods of decreased activity. Evoked responses in these cells were characteristic of striatal cells. Type I cells which were intracellularly labeled were found outside the grafts and displayed the characteristic morphology of the medium spiny neuron of the neostriatum. The other three cell classes displayed electrophysiological properties similar to neurons recorded in situ within the reticular formation, substantia nigra pars compacta and substantia nigra pars reticulata. Neurons from these three groups which were labeled with an intracellular marker were found to lie within the suspension grafts. The spontaneous activity of the pars compacta dopaminergic-like neurons was predominantly irregular, with some cells also firing in a regular or pacemaker-like pattern. Infrequently, irregular firing dopaminergic-like neurons displayed episodes of doublet bursting. Many of the grafted neurons responded to electrical stimulation of prefrontal cortex and striatum, indicating that the graft was receiving functional inputs from host neurons. Comparison of the firing rate and pattern of grafted neurons to in situ mesencephalic neurons as a function of time following grafting suggested that the grafted neurons and/or the neuronal circuitry is slowly developing within the host environment. A prolonged time-course for the maturation of the graft may be reflected in the time required to achieve improvements in some behavioral deficits following transplantation. However, the relatively rapid recovery of drug-induced rotational asymmetry following grafting suggests that this form of recovery may not require mature functioning of the grafted neurons.
Assuntos
Transplante de Tecido Fetal , Mesencéfalo/fisiologia , Neurônios/fisiologia , Animais , Corpo Estriado/fisiologia , Estimulação Elétrica , Eletrofisiologia , Feminino , Mesencéfalo/citologia , Ratos , Ratos EndogâmicosRESUMO
The cochleae from 3 lines of mice, selectively bred for differential susceptibility to priming-induced audiogenic seizures, were examined following acoustic priming and retest or kanamycin treatment, and the degree of cochlear damage was assessed. After 60 sec of acoustic priming, animals from the high and unselected lines which had subsequently developed audiogenic seizure susceptibility exhibited severe cochlear damage limited to the outer hair cells. Low line mice, which had been selected for resistance to acoustic priming-induced audiogenic seizures and were not seizure susceptible, exhibited no cochlear pathology following acoustic priming. Following kanamycin treatment, all 3 lines developed subsequent audiogenic seizure susceptibility. Histological examination of cochleae from mice so treated revealed a pattern of damage similar to that caused by acoustic priming, except that the cochleae of priming-induced audiogenic seizure resistant low line mice revealed a significant amount of outer hair cell damage. The results are discussed with respect to the physiological mechanism underlying a selectively bred behavioral phenotype in terms of a possible instance of damage/disuse-supersensitivity in the central nervous system.
Assuntos
Cóclea/efeitos dos fármacos , Canamicina/toxicidade , Convulsões/induzido quimicamente , Estimulação Acústica , Animais , Células Ciliadas Auditivas/efeitos dos fármacos , Células Ciliadas Auditivas/ultraestrutura , Camundongos , Degeneração Neural/efeitos dos fármacos , Convulsões/genética , Seleção GenéticaRESUMO
The effect of medial forebrain bundle (MFB) stimulation on neostriatal dopamine levels was examined using in vivo microdialysis in urethane-anesthetized and awake, freely-moving rats in conjunction with single unit extracellular recordings from antidromically identified nigral dopaminergic neurons. Dialysis samples were collected during baseline periods or while stimulating the MFB with trains of 5 or 10 pulses at different frequencies within a physiologically relevant range. When the perfusion solution contained 1.2 mM Ca2+, even intense, high frequency stimulation was ineffective at producing significant elevations in neostriatal dopamine levels whereas cocaine or amphetamine reliably caused several-fold elevations in dopamine levels. When the perfusate contained 2.4 mM Ca2+, modest MFB stimulation within the range of spontaneous nigral cell firing produced large and reliable increases in dopamine levels. There was a significant correlation between the proportion of dopaminergic neurons that could be antidromically activated from the MFB and the increase in neostriatal dopamine. There was no effect of stimulus pattern on the increase in dopamine levels, and results obtained in awake, freely-moving animals did not differ from those obtained in anesthetized animals. These data provide good evidence that in vivo microdialysis is sensitive to neostriatal dopamine overflow evoked by stimulation within the normal rate of firing of nigrostriatal neurons and that Ringer's Ca2+ concentration is a critical variable in the detection of stimulus-induced release of dopamine.
Assuntos
Corpo Estriado/metabolismo , Dopamina/metabolismo , Anestesia , Animais , Cálcio/metabolismo , Corpo Estriado/fisiologia , Diálise , Estimulação Elétrica , Eletrofisiologia , Masculino , Feixe Prosencefálico Mediano/fisiologia , Microeletrodos , Neurônios/fisiologia , Ratos , Ratos Endogâmicos , Substância Negra/citologia , Substância Negra/metabolismoRESUMO
The effects of the GABAA agonist, isoguvacine, on NMDA-induced burst firing of substantia nigra dopaminergic neurons were studied with intracellular and whole cell recordings in vitro. NMDA application caused the neurons to fire in rhythmic bursts. Although the NMDA-induced bursty firing pattern was insensitive to hyperpolarization by current injection, it was reversibly abolished by the selective GABAA agonist, isoguvacine. The block of the rhythmic burst pattern by isoguvacine application occurred regardless of whether the chloride reversal potential was hyperpolarizing (ECl-=-70 mV) or depolarizing (ECl-=-40 mV). In either case, the input resistance of the dopaminergic neurons was dramatically decreased by application of isoguvacine. It is concluded that GABAA receptor activation by isoguvacine disrupts NMDA receptor-mediated burst firing by increasing the input conductance and thereby shunting the effects of NMDA acting at a distally located generator of rhythmic burst firing.
Assuntos
Antagonistas de Aminoácidos Excitatórios/farmacologia , Agonistas GABAérgicos/farmacologia , Agonistas de Receptores de GABA-A , Ácidos Isonicotínicos/farmacologia , Neurônios/efeitos dos fármacos , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Animais , Dopamina/fisiologia , Condutividade Elétrica , Impedância Elétrica , Técnicas In Vitro , Potenciais da Membrana/efeitos dos fármacos , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-Dawley , Estimulação QuímicaRESUMO
Experiments were carried out to explore the view that activation of presynaptic receptors on the terminals of noradrenergic neurons is accompanied by alterations in their excitability to direct electrical stimulation. Antidromic action potentials evoked from frontal cortex of urethane anesthetized rats were recorded extracellularly from nucleus locus coeruleus. The threshold current necessary to evoke antidromic action potentials varied as a result of infusion of adrenergic agonists and antagonists into frontal cortex within 50 micrometer of the stimulating electrode. Local infusion of the alpha-adrenergic agonist clonidine produced a marked decrease in terminal excitability, while the alpha-antagonist phentolamine produced an increase in terminal excitability and was shown to reverse the effect of the agonist. Infusion of the beta-adrenergic agonist isoproterenol was without effect, although the beta-antagonist propranolol resulted in a decrease in terminal excitability. Infusions of potassium increased excitability of locus coeruleus terminals. Terminal excitability was seen to vary inversely with the rate of spontaneous or high frequency stimulation-induced firing of locus coeruleus neurons. From these observations, it may be inferred that activation or blockade of alpha-adrenergic presynaptic receptors results in changes in polarization and/or conductance of the noradrenergic synaptic endings. These results are discussed with respect to phenomena associated with the possible presynaptic regulation of neurotransmitter release.
Assuntos
Lobo Frontal/fisiologia , Locus Cerúleo/fisiologia , Norepinefrina/fisiologia , Receptores Adrenérgicos/fisiologia , Animais , Clonidina/farmacologia , Estimulação Elétrica , Potenciais Evocados/efeitos dos fármacos , Isoproterenol/farmacologia , Masculino , Neurônios/fisiologia , Fentolamina/farmacologia , Potássio/farmacologia , Propranolol/farmacologia , Ratos , Ratos Endogâmicos , Transmissão Sináptica/efeitos dos fármacosRESUMO
The effect of spontaneous and stimulation-induced alterations in impulse flow on the antidromic excitability of nigrostriatal dopaminergic neurons were investigated in urethane-anesthetized rats. Terminal excitability was found to be inversely related to the rate of spontaneous activity of nigral neurons. Conditioning stimulation applied to dopaminergic axons in the medial forebrain bundle was found to decrease terminal excitability, but axonal conditioning stimulation was without effect on antidromic responses evoked from the medial forebrain bundle. Decreases in terminal excitability induced by medial forebrain bundle stimulation could be blocked by local infusions of haloperidol into the region of the terminal fields, suggesting that the effect was receptor-mediated. These results are consistent with the proposal that nigrostriatal dopaminergic neurons may modulate the impulse-dependent release of dopamine from striatal nerve terminals as a function of firing rate by autoreceptor-mediated alterations in the electrical properties of the terminal membrane.