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Objective: To investigate the association between long-term fasting blood glucose (FPG) variability and all-cause mortality in patients with type 2 diabetes. Methods: A total of 7 174 type 2 diabetic patients included in National Basic Public Health Service Program in Changshu of Jiangsu Province were recruited as participants. Long-term glucose variability was assessed using standard deviation (SD), coefficient of variation (CV), average real variability (ARV), and variability independent of the mean (VIM) across FPG measurements at the more than three visits. Death information were mainly obtained from the death registry system in Jiangsu. Then Cox proportional hazards regression models were used to estimate the associations of four variability indicators and all-cause mortality's hazard ratios (HRs) and their 95%CIs. Results: Among 55 058.50 person-years of the follow-up, the mean follow-up time was 7.67 years, and 898 deaths occurred during the follow-up period. After adjustment, compared with T1 group, the Cox regression model showed that HRs of T3 group in SD, CV, ARV and VIM were 1.24 (95%CI: 1.03-1.49), 1.20 (95%CI: 1.01-1.43), 1.28 (95%CI: 1.07-1.55) and 1.20 (95%CI:1.01-1.41), respectively. HRs of per 1 SD higher SD, CV, ARV and VIM were 1.13 (95%CI: 1.06-1.21), 1.08 (95%CI: 1.01-1.15), 1.05 (95%CI: 1.00-1.12) and 1.09 (95%CI: 1.02-1.16) for all-cause mortality, respectively. In the stratified analysis, age, gender, hypoglycemic agent and insulin uses had no effect on the above associations (all P for interaction >0.05). Conclusion: Long-term FPG glycemic variability was positively associated with the risk of all-cause mortality in type 2 diabetes patients.
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ObjectiveTo study the anti-tumor activity and mechanism of Lycopus lucidus polysaccharide （LLP） in vitro. MethodCell counting kit-8 （CCK-8） assay was used to detect the inhibitory effect of LLP （0， 5， 10， 15， 20 g·L-1） on the proliferation of A549 cells at different time points （24，48，72 h）. The migration and invasion abilities of A549 cells were detected by wound healing assay and transwell assay after LLP （10， 20 g·L-1） treatment for 24，48 h. Propidium iodide （PI） single staining was applied to determine the effect of LLP of different concentrations （10，20 g·L-1） on the cell cycle of A549. The apoptosis of A549 cells induced by LLP （10， 20 g·L-1） was detected by Annexin V-FITC/PI kit. Real-time fluorescence-based quantitative polymerase chain reaction （Real-time PCR） was adopted to measure effect of LLP （10， 20 g·L-1） on gene expression of cysteine aspartate protease-3 （Caspase-3），cysteine aspartate protease-8 （Caspase-8），cysteine aspartate protease-9 （Caspase-9），cyclin-dependent kinase-1 （CDK-1）， and Cyclin B1 in A549 cells. Western blot was used to detect the effect of LLP on protein expression of Caspase-3，Caspase-8，Caspase-9，B-cell lymphoma 2 （Bcl-2）-associated X protein （Bax），CDK-1，cyclin-dependent kinase-4 （CDK-4），cyclin-dependent kinase-6 （CDK-6），Cyclin B1，and Cyclin D1 in A549 cells. ResultCompared with the blank group， the LLP group showed decreased proliferation， migration， and invasion of A549 cells （P<0.05， P<0.01）， increased proportion of G0/G1 phase （P<0.05）， enhanced apoptosis rate （P<0.05， P<0.01）， elevated mRNA expression of Caspase-3，Caspase-8，and Caspase-9 （P<0.05，P<0.01）， reduced mRNA expression of CDK-1 and Cyclin B1 （P<0.05，P<0.01）， up-regulated protein expression of Caspase-3，Caspase-8，Caspase-9， and Bax （P<0.05， P<0.01）， and down-regulated protein expression of Bcl-2， CDK-1， CDK-4， CDK-6， Cyclin B1， and Cyclin D1 （P<0.05， P<0.01）. ConclusionLLP can inhibit the proliferation of A549 cells， block the cell cycle in the G0/G1 phase （also G2/M phase）， and induce cell apoptosis via the mitochondrial apoptosis pathway and death receptor pathway.
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Nelumbinis Folium is a Chinese herbal medicine with the effect of regulating lipid and losing weight recorded in “Compendium of Materia Medica”. The alkaloids extracted from lotus leaves have bacteriostatic, lipid-lowering, antispasmodic and other biological activities. In recent years, much attention has been paid to the health problems caused by obesity, so the effect of alkaloids in Nelumbinis Folium on lowering fat and losing weight has become a research hotspot. The chemical constituents, lipid-lowering mechanisms and application in medicine of alkaloids in Nelumbinis Folium were reviewed, which provides a theoretical reference for the development of slimming products and lipid-lowering mechanism of alkaloids in Nelumbinis Folium.
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Objective To learn of the epidemiological characteristics of pesticide poisoning in western Anhui Province, and to provide the basis for control and prevention. Methods The pesticide poisoning report cards in western Anhui Province during 2006-2016 were collected from Occupational Disease and Occupational Health Information Monitoring System. Descriptive analysis was conducted on the general situation of pesticide poisoning, the distribution of three cases and the types of pesticide poisoning. Results There were totally 2993 cases with pesticide poisoning in western Anhui Province from 2006 to 2016, of which 280 cases (9.36％) were industrial poisoning and 2713 cases (90.64％) were non-industrial poisoning. The average annual incidence rate was 4.63/10000, and showed an increasing trend year by year (P<0.01) . One hundred cases died due to pesticide poisoning, and the fatality rate was 3.34％. Both the industrial and non-industrial poisoning cases were the highest in the third quarter (from July to September), accounting for 78.21％ and 28.86％ respectively. The cases of industrial poisoning were mainly distributed in 45-<55 years old group, and the non-industrial poisoning cases were mainly distributed in 35-<45 years old, accounting for 29.64％ and 20.94％ respectively. The number of cases of industrial poisoning and non-industrial poisoning was 2.33:1 and 1:1.57 respectively. The types of pesticide poisoning involve seven kinds of insecticides, fungicides and rodenticide, and organic phosphorus poisoning was accounted for 30.84％. The highest mortality rate of pesticide poisoning was bisultap (10.93％) . Conclusion The non-industrial pesticide poisoning is the main type in western Anhui Province of pesticide poisoning, and organ phosphorus insecticides are the main types of pesticide poisoning.
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To treat respiratory distress syndrome, surfactant is currently delivered via less invasive surfactant administration (LISA) or INtubation SURfactant Extubation (INSURE). The aim of this study was to compare the effect of the two delivery methods of surfactant on cerebral autoregulation. Near infrared spectroscopy monitoring was carried out to detect cerebral oxygen saturation (ScO), and the mean arterial blood pressure (MABP) was simultaneously recorded. Of 44 preterm infants included, the surfactant was administrated to 22 via LISA and 22 via INSURE. The clinical characteristics, treatments and outcomes of the infants showed no significant differences between the two groups. The correlation coefficient of ScOand MABP (r) 5 min before administration was similar in the two groups. During surfactant administration, rincreased in both groups (0.44±0.10 to 0.54±0.12 in LISA, 0.45±0.11 to 0.69±0.09 in INSURE). In the first and second 5 min after instillation, rwas not significantly different from baseline in the LISA group, but increased in the first 5 min after instillation (0.59±0.13, P=0.000 compared with the baseline in the same group) and recovered in the second 5 min after instillation (0.48±0.10, P=0.321) in the INSURE group. There were significant differences in the change rates of rbetween the two groups during and after surfactant administration. Our results suggest that cerebral autoregulation may be affected transiently by surfactant administration. The effect duration of LISA is shorter than that of INSURE (<5 min in LISA vs. 5-10 min in INSURE).
Assuntos
Feminino , Humanos , Recém-Nascido , Masculino , Administração Intranasal , Encéfalo , Metabolismo , Homeostase , Recém-Nascido Prematuro , Intubação , Consumo de Oxigênio , Surfactantes Pulmonares , Usos Terapêuticos , Síndrome do Desconforto Respiratório do Recém-Nascido , Tratamento Farmacológico , TerapêuticaRESUMO
<p><b>OBJECTIVE</b>To explore the different expression of proteins between human clear-cell renal cell carcinoma (ccRCC) cell line RLC-310 and normal renal proximal tubule epithelial cell line HK-2, and to search new differentially expressed proteins of RCC.</p><p><b>METHODS</b>RLC-310 and HK-2 cells were cultured in vitro. The total proteins were separated by ProteomeLab PF 2D protein fractionation system and the differential expression protein fractions of the two cell lines were analyzed and identified by capillary liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS). RT-PCR and Western blot were used to confirm the representative differential expression at mRNA and protein levels.</p><p><b>RESULTS</b>One hundred and ninty-six differentially expressed proteins were identified. These differentially expressed proteins involved in many aspects, including cell proliferation and anti-apoptosis, energy metabolism, mitochondria reduction and oxidation, oxidative stress and resistance, cell signaling, invasion and adhesion, cytoskeleton and motion, neovascularization, etc. Except for previously reported RCC associated proteins: annexin A2, fatty acid-binding protein, vimentin, fibronectin, and so on, Septin-9 was firstly found highly expressed in RLC-310 cells when compared with that in the HK-2 cells. Moreover, the overexpression of Septin-9 was confirmed by RT-PCR and Western blot analysis at both mRNA and protein levels (P < 0.05).</p><p><b>CONCLUSIONS</b>The human ccRCC cell line RLC-310 cells display differential protein profiles compared with those of the normal renal cell line HK-2 cells. The identified differential expression proteins are involved in many aspects of RCC development. It is worth further study and elucidate the molecular mechanisms of RCC. The representative differential protein Septin-9 deserves further study its role in the angiogenesis of ccRCC.</p>