RESUMO
In this study we examined by QRT-PCR the mRNA expression of TGF-beta 1, IGF-1, EGF, FGF-2 and YY1 in human brain tumors. Our findings introduce YY1, for the first time, as a novel gene implicated in brain gliomatogenesis and meningioma establishment. We present a positive correlation between the autocrine expression of YY1 and TGF-beta 1, IGF-1 and FGF-2, known to be involved in the progression of gliomas and meningiomas. We suggest that mRNA profiling of the above genes in the early stages of disease development could be useful for prognostic purposes, and these genes can be considered as potential targets for therapeutic approaches against brain tumors.
Assuntos
Neoplasias Encefálicas/metabolismo , Fator 2 de Crescimento de Fibroblastos/genética , Glioma/metabolismo , Fator de Crescimento Insulin-Like I/genética , Meningioma/metabolismo , RNA Mensageiro/análise , Fator de Crescimento Transformador beta1/genética , Fator de Transcrição YY1/genética , Adulto , Idoso , Neoplasias Encefálicas/etiologia , Feminino , Glioma/etiologia , Humanos , Masculino , Meningioma/etiologia , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Major Glioblastoma's hallmarks include proliferation, invasion and heterogeneity. Biological 3D tumor spheroid models can serve as intermediate systems between traditional 2D cell culture and complex in vivo models. Tumor spheroids have been shown to more accurately reproduce the spatial organization and microenvironmental factors of in vivo micro-tumors, such as relevant gradients of nutrients and other molecular agents, while they maintain cell-to-cell and cell-to-matrix interactions. In vitro 3D assays are useful to monitor these properties. Here, we test the suitability of the well-known T98â¯G Glioblastoma cell line in such a 3D assay. The doubling time and death rate parameters of T98â¯G are estimated, as well as their spheroidal growth-expansion curves with and without the presence of basement membrane substrate. The T98â¯G invasive profile is characterized by collective morphology and proliferation-associated invasion. We show that the T98â¯G secondary GB cell line exhibits both invasive and proliferative capabilities in 3D and thus, can serve as control cell line for the 3D in vitro study of primary GB cell cultures.
Assuntos
Glioblastoma , Modelos Biológicos , Esferoides Celulares , Linhagem Celular Tumoral , Glioblastoma/metabolismo , Glioblastoma/patologia , Humanos , Esferoides Celulares/metabolismo , Esferoides Celulares/patologiaRESUMO
We report on a case of a 13-month-old female infant who was admitted to our department with the chief complaints of drowsiness and vomiting. A history of an unspecified viral infection was reported. Clinical examination was negative for focal neurological signs or signs of central nervous system infection. Initial CT scan revealed obstructive hydrocephalus, and shunting was scheduled. Dexamethasone treatment was started. Eight hours after admission the child almost restored his baseline mental status and the operation was postponed. The dexamethasone treatment was discontinued 3 days later. Follow up CT and MRI scans were normal. We discuss the case and the possible causes of transient hydrocephalus in children.