RESUMO
The purpose of our approach was to take into account the nested spatial scales driving stream functioning in the description of pressures/ecological status links by analysing the results of a hierarchical model. The development of this model has allowed us to answer the following questions: Does the consideration of the indirect links between anthropogenic pressures and stream ecological status modify the hierarchy of pressure types impacting benthic invertebrates? Do the different nested scales play different roles in the anthropogenic pressures/ecological status relationship? Does this model lead to better understanding of the specific role of hydromorphology in the evaluation of stream ecological status? To achieve that goal, we used the Partial Least Square (PLS) path modelling method to develop a structural model linking variables describing (i) land use and hydromorphological alterations at the watershed scale, (ii) hydromorphological alterations at the reach scale, (iii) nutrients-organic matter contamination levels at the site scale, and (iv) substrate characteristics at the sampling site scale, to explain variation in values of a macroinvertebrate-based multimetric index: the French I2M2. We have highlighted the importance of land use effects exerted on both hydromorphological and chemical characteristics of streams observed at finer scales and their subsequent indirect impact on stream ecological status. Hydromorphological alterations have an effect on the substrate mosaic structure and on the concentrations of nutrients and organic matter at site scale. This result implies that stream hydromorphology can have a major indirect effect on macroinvertebrate assemblages and that the hierarchy of impacts of anthropogenic pressures on stream ecological status generally described in the literature - often determining strategic restoration priorities - has to be re-examined. Finally, the effects of nutrients and organic matter on macroinvertebrate assemblages are lower than expected when all the indirect effects of land use and hydromorphological alterations are taken into account.
Assuntos
Ecossistema , Monitoramento Ambiental , Invertebrados , Rios , Animais , Ecologia , Análise dos Mínimos Quadrados , Modelos TeóricosRESUMO
Stimulation of rat thymocytes with the lectin ConA produced an early peak of ornithine decarboxylase (ODC) activity within 10 min. This ODC induction appeared as early as the well-known inositol phosphate accumulation following mitogenic stimulation, and may be part of the signal transduction mechanism. The distribution of counts among the inositol phosphates was constant during the overall time of Concanavalin A (ConA) stimulation. We conclude that early induction of pre-existing ODC may be independent of protein kinase C action.
Assuntos
Fosfatos de Inositol/metabolismo , Ornitina Descarboxilase/biossíntese , Linfócitos T/metabolismo , Animais , Concanavalina A/farmacologia , Ativação Enzimática , Indução Enzimática , Ativação Linfocitária , Masculino , Proteína Quinase C/metabolismo , Ratos , Ratos Endogâmicos , Transdução de Sinais , Linfócitos T/efeitos dos fármacosRESUMO
The discovery of the role played by somatic antigens of the Pasteurella multocida A and D capsular types in the immunization of susceptible species makes it necessary to type the somatic antigens of the isolated strains prior to any action to induce active or passive immunity. Only immunoprecipitation in gel was chosen out of the various typing techniques proposed, and a correlation was established between the capsular types (according to Carter), somatic types (according to Namioka) and types determined by immunoprecipitation in gel (according to Heddleston). Parallel to this, the same technique was developed for strains of Pasteurella multocida B and E capsular types, and the immunogenicity of these capsular antigens was proven.
Assuntos
Antígenos de Bactérias/imunologia , Vacinas Bacterianas/normas , Infecções por Pasteurella/prevenção & controle , Pasteurella/imunologia , Testes de Aglutinação , Animais , Antígenos de Bactérias/análise , Antígenos de Bactérias/classificação , Bovinos , Epitopos , Testes de Hemaglutinação , Ácido Hialurônico/análise , Imunidade , Imunodifusão , Mamíferos , Aves Domésticas , VacinaçãoRESUMO
Blackleg (Clostridium chauvoei) infection in Charollais cattle appears to have undergone some etiological and pathogenic changes which are reflected in the apparent failure of vaccination. Two methods have been used to determine the quality of the vaccines in order to meet the different requirements specified by pharmacopeias. The two methods differ in the vaccination schedules of the guinea pigs and the test strain used which may be either a virulent culture or a suspension of spore in calcium chloride. Both methods appear to be efficient in selecting vaccines which provide good protection. It follows, therefore, that the vaccination failures would appear to be the result of intensive selection of beef cattle with reduced immunological response. If this is the case, the remedy for these vaccination failures might lie in an adaptation of the vaccination schedule to the early developing breeds of cattle obtained by intensive selective breeding.
Assuntos
Vacinas Bacterianas/normas , Doenças dos Bovinos/prevenção & controle , Infecções por Clostridium/veterinária , Clostridium/imunologia , Vacinação/veterinária , Animais , Vacinas Bacterianas/administração & dosagem , Bovinos , Infecções por Clostridium/prevenção & controle , Cobaias , MétodosRESUMO
The activity of the antibrucellic vaccines was tested on mice by vaccination at varying doses. The vaccinated mice and the controls were then challenged with a fixed quantity of virulent brucella. The animals were then sacrificed, the spleens were weighed and a count was taken of the virulent brucella which they contained. These two indications, weight of the spleen and intensity of infection, allow to compare the activity of the vaccines and to have an indispensible routine test for production control.
Assuntos
Vacina contra Brucelose/normas , Brucella abortus/crescimento & desenvolvimento , Brucelose/prevenção & controle , Animais , Brucella abortus/imunologia , Brucelose/microbiologia , Tamanho do Órgão , Baço/anatomia & histologia , Baço/microbiologia , Fatores de TempoRESUMO
Judged from its biochemical properties and antigenic structure, Mycobacterium paratuberculosis is related to M. avium. When M. paratuberculosis was compared to B.C.G., several essential differences, were found that allowed their clear separation, within Runyon's Group III, M. paratuberculosis is differentiated by its biochemical properties as shown in the following table : (formula: see text). The allergic response of a guinea pig infected with M. paratuberculosis was stronger when an avium sensitine was used that when a bovine tuberculine was used.
Assuntos
Antígenos de Bactérias , Mycobacterium/metabolismo , Animais , Cobaias , Mycobacterium/crescimento & desenvolvimento , Mycobacterium/imunologia , Testes Cutâneos , Teste TuberculínicoRESUMO
The early biochemical responses to concanavalin A (Con A) of thymocytes from rats fed a saturated (coconut oil), (n-6) (sunflower oil) or (n-3) (fish oil) fatty acid-enriched diet for 3 wk were investigated. Fish oil feeding resulted in greater (n-3) polyunsaturated fatty acid level (PUFA) at the expense of (n-6) PUFA in total and individual thymocyte phospholipids. Such alterations of the fatty acid composition did not affect basal ornithine decarboxylase (ODC), cyclic nucleotide phosphodiesterase (PDE) or gamma-glutamyl transferase activities. However, the fish oil-enriched diet impaired some of the early thymocyte responses to Con A, such as the rapid induction (30 min) of soluble ODC and PDE activities. Synthesis of [3H]20:4(n-6) oxygenated metabolites was not different between the dietary groups; however, the uptake of [3H]20:4(n-6) into phospholipid classes was significantly lower in phosphatidylcholine and greater in phosphatidylethanolamine and phosphatidylinositol after fish oil feeding. Similarly, the Con A-induced remodeling of the [3H]20:4(n-6) esterification in phospholipids differed in sunflower oil- vs. fish oil-fed rats, suggesting a modulation of acyl CoA synthase and/or acyl CoA transferase activities. Thus, the modulation of Con A-induced ODC and PDE stimulation upon in vivo changes of membrane phospholipid fatty acid composition is not related to eicosanoid formation, but rather to the modification of the fatty acid acylation processes, altering phospholipid composition and signal transduction.
Assuntos
Concanavalina A/farmacologia , Gorduras na Dieta/farmacologia , Ácidos Graxos Insaturados/fisiologia , Ativação Linfocitária/efeitos dos fármacos , Linfócitos T/imunologia , 2',3'-Nucleotídeo Cíclico Fosfodiesterases/efeitos dos fármacos , Animais , Óleo de Coco , Gorduras na Dieta/imunologia , Ácidos Graxos Insaturados/imunologia , Óleos de Peixe/farmacologia , Interfase , Ornitina Descarboxilase/efeitos dos fármacos , Fosfolipídeos/imunologia , Óleos de Plantas/farmacologia , Ratos , Ratos Endogâmicos , Óleo de Girassol , Linfócitos T/efeitos dos fármacos , Linfócitos T/enzimologia , gama-Glutamiltransferase/efeitos dos fármacosRESUMO
Pretreatment of rat thymic lymphocytes with Concanavalin A induced a very early (30 min) and substantial increase (+90%) of the soluble cAMP phosphodiesterase activity. The crude cytosolic phosphodiesterase activity of rat thymocytes could reproducively be resolved by Mono-Q ion exchange high performance liquid chromatography into four separate phosphodiesterase peaks: a cGMP-stimulated, two cAMP-specific Rolipram-sensitive and a cGMP-inhibited cardiotrope-sensitive peaks. Concanavalin A stimulated very specifically the activity of the two predominant cAMP-specific Rolipram sensitive peaks whereas it only slightly modified the cGMP-stimulated and the cGMP-inhibited forms. The present results strongly suggest that the Rolipram-sensitive cAMP PDE activity may play a key role in the control and regulation of mitogen-induced thymocyte proliferation.
Assuntos
3',5'-AMP Cíclico Fosfodiesterases/metabolismo , 3',5'-GMP Cíclico Fosfodiesterases/metabolismo , Concanavalina A/farmacologia , Linfócitos/enzimologia , Pirrolidinonas/farmacologia , 3',5'-AMP Cíclico Fosfodiesterases/antagonistas & inibidores , 3',5'-AMP Cíclico Fosfodiesterases/isolamento & purificação , 3',5'-GMP Cíclico Fosfodiesterases/antagonistas & inibidores , 3',5'-GMP Cíclico Fosfodiesterases/isolamento & purificação , Animais , AMP Cíclico/metabolismo , GMP Cíclico/metabolismo , Citosol/enzimologia , Isoenzimas/isolamento & purificação , Isoenzimas/metabolismo , Cinética , Ativação Linfocitária , Milrinona , Piridonas/farmacologia , Ratos , Ratos Endogâmicos , Rolipram , Timo/citologiaRESUMO
Combined vaccines are used for many human and animal diseases. However, we have very few examples of combinations containing anti-foot-and-mouth valence which would allow easy immunization against differnet diseases without additional handling. Until now foot-and-mouth vaccination has been applied simultaneously with anti-brucellic vaccination (Baldoni, Renoux), andi-anthrax (Nobili) and vaccination against swine fever and Aujeszky's disease (Lysenko). During the last ten years we have particularly studied the association of anti-foot-and-mouth disease vaccines with anti-brucella and anti-rabies vaccines. We have shown the possiblity of combining these different antigens, either in classical aqueous vaccines in aluminum hydroxide, or in emulsified vaccines containing oily adjuvants. The anti-brucella valences may be agglutinogenic (H 38) or non-agglutinogenic (MacEwen 45/20). The combined vaccines "rabies + foot-and-mouth disease" and "brucellosis + foot-and-mouth disease" can be produced on an industrial scale. For many years millions of animals have been successfully vaccinated in France by means of a combined "rabies + foot-and-mouth disease" vaccine.
Assuntos
Aphthovirus/imunologia , Vacinas Virais , Adjuvantes Imunológicos/administração & dosagem , Hidróxido de Alumínio/administração & dosagem , Animais , Vacina contra Brucelose/administração & dosagem , Brucella abortus/imunologia , Brucelose Bovina/prevenção & controle , Bovinos , Doenças dos Bovinos/prevenção & controle , Febre Aftosa/prevenção & controle , Óleos/administração & dosagem , Raiva/prevenção & controle , Raiva/veterinária , Vacina Antirrábica/administração & dosagem , Saponinas/administração & dosagem , Vacinas Virais/administração & dosagemRESUMO
The B 19 brucellosis strain has been widely used in the production of live vaccine against Bovine Brucellosis in the field for the last 30 years. Many attempts have been made to stabilize this vaccine but it remains a problem for laboratories which prepare this vaccine. In order to improve our knowledge of the influence of residual moisture and the nature of the conditioning atomosphere, several batches of vaccine were prepared in the same freeze-drying apparatus, either with a different atmosphere, or with a different level of residual moisture. After freeze-drying, the percentage of live bacteria and the stability of the vaccine at 37 degrees and 4 degrees C were investigated. It is thus possible to determine under specific conditions the level of residual moisture which gives: a) the best stability and b) the correct percentage of live bacteria after freeze-drying. Furthermore, by using nitrogen, argon, or a vacuum in the freeze-drying procedure, it was possible to select the most favourable atmosphere for conditioning the vaccine.
Assuntos
Vacina contra Brucelose , Brucella abortus/imunologia , Liofilização , Vacina contra Brucelose/normas , Estabilidade de Medicamentos , Liofilização/métodos , ÁguaRESUMO
Medical and sanitary prevention of a growing number of major infectious diseases of bovines has led IFFA-Mérieux to investigate the association of vaccines and to define methods of utilization. Care has been taken to limit the number of interventions and at the same time to retain in the combined valences the efficacy they had when in a state of single constituent. The work which has been carried out for the last ten years, including the study of adjuvants as well as of incompatibility of associations, has permitted the improvement of formulas, reduced in volume (5 ml for a plurivalent dose), in aqueous excipient (aluminum hydroxide and saponin) or oily excipient (oil in water type). These formulas combine 3 foot-and-mouth disease valences with a rabies valence (fixed strain grown on cell culture) or with a brucellosis valence (strain B. abortus MacEwen 45/20 and B. melitensis H 38). The innocuity of these associations allows them to be used in sanitary compaigns; their efficacy is equal to that of monovalent vaccines. A logical vaccination calendar is proposed.